##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926742_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	758481
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.646028048164688	31.0	30.0	33.0	23.0	34.0
2	28.695292301323303	31.0	30.0	31.0	23.0	34.0
3	28.412067012884965	31.0	28.0	33.0	16.0	34.0
4	32.27521058536733	35.0	33.0	37.0	23.0	37.0
5	32.19578473290696	35.0	33.0	37.0	22.0	37.0
6	31.735467335371617	35.0	32.0	37.0	17.0	37.0
7	31.75021127754024	35.0	32.0	37.0	17.0	37.0
8	31.590137393026325	35.0	32.0	37.0	17.0	37.0
9	33.7676527164161	38.0	34.0	39.0	22.0	39.0
10	33.905276467044	38.0	34.0	39.0	23.0	39.0
11	34.058014637149775	38.0	35.0	39.0	25.0	39.0
12	33.923499731700595	38.0	35.0	39.0	23.0	39.0
13	34.03363828494056	38.0	35.0	39.0	25.0	39.0
14	35.09845599296489	39.0	36.0	41.0	25.0	41.0
15	34.6786089565856	39.0	34.0	41.0	19.0	41.0
16	34.7820011312083	39.0	34.0	41.0	22.0	41.0
17	35.01440115177572	39.0	35.0	41.0	25.0	41.0
18	35.06164162319162	39.0	36.0	41.0	25.0	41.0
19	34.44547193667343	39.0	34.0	41.0	18.0	41.0
20	34.720702298409584	39.0	34.0	41.0	19.0	41.0
21	34.717222975921615	39.0	35.0	41.0	19.0	41.0
22	34.74011478204464	39.0	35.0	41.0	18.0	41.0
23	34.74999373748321	39.0	35.0	41.0	19.0	41.0
24	33.46971908327301	39.0	33.0	41.0	2.0	41.0
25	34.0580897873513	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.27918255476861376
1101	2	-0.43985782887613567
1101	3	-1.0702468071321256
1101	4	-0.5806061125927506
1101	5	-0.5335252290139394
1101	6	-2.2309762306469914
1101	7	-0.8030391145716287
1101	8	-0.3774207866563266
1101	9	-0.2845896782303754
1101	10	-9.445676488472692
1101	11	-4.127242870819625
1101	12	-1.822359987462164
1101	13	-0.9900330830212454
1101	14	-0.6586932119178144
1101	15	-2.065154736738819
1101	16	-0.6076563010572045
1101	17	-0.37810319686079197
1101	18	-0.3401276005964462
1101	19	-0.9039665045688778
1101	20	-0.3451375935550445
1101	21	-0.3778457074928383
1101	22	-0.2687410126313452
1101	23	-0.471750833250951
1101	24	-0.6701257481202632
1101	25	-0.4704893436624218
1102	1	-0.04469381058374822
1102	2	0.04301727505054842
1102	3	-0.2695527990210813
1102	4	0.13169468137537876
1102	5	0.09217211109763213
1102	6	-0.6849628899515245
1102	7	0.3545184973042552
1102	8	0.6076328109522464
1102	9	0.5440740943558282
1102	10	0.5556705917428388
1102	11	0.3146264132127925
1102	12	0.4397494544753471
1102	13	0.37708514905100543
1102	14	0.28230499197523073
1102	15	0.832616313571215
1102	16	0.6128412142223993
1102	17	0.2055030431828726
1102	18	0.13146312819140604
1102	19	0.5755274121501515
1102	20	0.5007415151189463
1102	21	0.2039533189283418
1102	22	0.3476759126620266
1102	23	-0.31990998228975087
1102	24	-3.233774891637456
1102	25	-1.7442972808050285
1103	1	-0.13338173503798956
1103	2	-0.3191112286483957
1103	3	-0.059489006194493044
1103	4	0.12024262818062681
1103	5	-0.22719589711611832
1103	6	0.6726402644926424
1103	7	0.29179537244516496
1103	8	0.42738279757084285
1103	9	0.2741248463910182
1103	10	0.4196303210004402
1103	11	0.08432100890771466
1103	12	0.14103990890799878
1103	13	0.12176380870419479
1103	14	0.22920350574673165
1103	15	0.7134528381678678
1103	16	0.4142075777393188
1103	17	0.1333837361614414
1103	18	0.032448810894678104
1103	19	-1.6018808765379333
1103	20	-0.34123053152297445
1103	21	-0.003484473739291616
1103	22	0.226729060374943
1103	23	0.2596477661949024
1103	24	1.621999470231529
1103	25	0.8515760548315328
1104	1	-0.16680099110335433
1104	2	0.07076629240267351
1104	3	-0.7843644182330465
1104	4	-0.23080172357141748
1104	5	-0.1585756599693795
1104	6	-1.2800256835475352
1104	7	-0.09002181522570396
1104	8	0.2806267712628632
1104	9	0.41479598103975235
1104	10	0.4008819808713966
1104	11	0.23141291498635752
1104	12	0.1339973693444847
1104	13	0.016822470496698827
1104	14	-0.18950499747927552
1104	15	0.2710210686282579
1104	16	-0.388553666421835
1104	17	-0.15216640365827772
1104	18	-0.13117228547938709
1104	19	-1.5403195823853721
1104	20	-0.3790638726674658
1104	21	0.08707574247354444
1104	22	0.03298892196123404
1104	23	0.09259600946559488
1104	24	1.3863813733419335
1104	25	0.8023329559891437
1105	1	0.17151341054996294
1105	2	0.1190767719549477
1105	3	0.5235205133564591
1105	4	0.377406027103639
1105	5	0.24445868858472153
1105	6	0.040170119804351145
1105	7	0.47752883267521895
1105	8	0.69903952714224
1105	9	-0.14104870375908973
1105	10	0.3259110195040975
1105	11	0.2573214093521088
1105	12	0.27709070026421756
1105	13	0.14192844264977822
1105	14	-0.02177559228098147
1105	15	0.49647399587328067
1105	16	0.1074495617690161
1105	17	0.13140678123861704
1105	18	0.10901944492142235
1105	19	-0.5381413078081252
1105	20	0.21700120403978218
1105	21	0.18307897066439693
1105	22	0.1453194042288075
1105	23	-0.2709050691121959
1105	24	-4.952184193627943
1105	25	-2.525869967484631
1106	1	0.1377273454448229
1106	2	-0.6863431041252959
1106	3	-0.3950928098928266
1106	4	-0.5863266615708653
1106	5	-0.04280914945224623
1106	6	0.2140681898794874
1106	7	-2.5116255628145687
1106	8	-0.2809604428457071
1106	9	-0.7339134889703516
1106	10	0.1250208621058988
1106	11	-0.1298895558504256
1106	12	-0.05507216312543051
1106	13	-0.2970105413523001
1106	14	-0.2634326175766404
1106	15	0.2710669949624176
1106	16	0.3412479318145927
1106	17	-0.09678383729472273
1106	18	-0.1572722211825166
1106	19	0.30732439667222167
1106	20	0.01858929165695855
1106	21	-0.15642108860939885
1106	22	-0.08899142156774786
1106	23	-0.2984621332734534
1106	24	-3.470645355784548
1106	25	-1.6919078817071878
1107	1	-0.23180324031612898
1107	2	-0.47131647862746817
1107	3	-0.15741884918221416
1107	4	-0.17965462505589613
1107	5	-0.40700121102650755
1107	6	-0.4457026965929707
1107	7	-2.9094999978617793
1107	8	-2.02355676449962
1107	9	-1.2417042438815216
1107	10	-0.7039763187237398
1107	11	-1.0671026045817982
1107	12	-0.7620440161705844
1107	13	-0.9560520234304306
1107	14	-0.8831847095800427
1107	15	-2.208241189581365
1107	16	-1.1887560955716339
1107	17	-0.978369732493185
1107	18	-1.2401916287092547
1107	19	-0.12493496700075468
1107	20	-0.31534367473673797
1107	21	-0.7866309601805312
1107	22	-0.9011541279712887
1107	23	-0.9798511340341847
1107	24	-4.8424760265681
1107	25	-2.626726873070684
1108	1	0.5363765957208493
1108	2	0.012191283451723933
1108	3	0.5529073325725378
1108	4	0.5254997443841916
1108	5	0.6126917943807797
1108	6	-2.719320348782052
1108	7	-1.3034124346313583
1108	8	0.3235913255056104
1108	9	0.20261373972217456
1108	10	0.37382326264727084
1108	11	0.2626069715563517
1108	12	0.41032210976696604
1108	13	0.23798251346125454
1108	14	0.07637398569293907
1108	15	-0.9472478178737092
1108	16	0.07838152870884585
1108	17	0.05260445205242803
1108	18	0.10652911086862815
1108	19	-0.027224344290132763
1108	20	-1.178159826838602
1108	21	-0.34683940253182755
1108	22	0.06807664125948065
1108	23	0.23297373950497757
1108	24	-0.5310640258703856
1108	25	-0.010932350133302293
1109	1	0.33563222762185063
1109	2	0.19821493914515642
1109	3	-1.1428674597564523
1109	4	0.21569391250369563
1109	5	0.4578529744306934
1109	6	0.5483530522366493
1109	7	0.7052704355016175
1109	8	0.9806179552529883
1109	9	0.7332302576861451
1109	10	0.8043731207814773
1109	11	0.4621719734364973
1109	12	-0.1566752922088952
1109	13	0.33769878187816715
1109	14	0.2271704851972629
1109	15	-1.1205420099223886
1109	16	-0.009848738272317803
1109	17	-0.12853508919120316
1109	18	0.008530614987861895
1109	19	-1.2609225974607483
1109	20	-0.31340663913135813
1109	21	-0.07911045165499075
1109	22	0.32352052735662795
1109	23	0.38709041129932587
1109	24	-0.5653553806032292
1109	25	-0.22497022659042187
1110	1	0.4999274393609028
1110	2	0.5686273532694237
1110	3	0.9311397655289575
1110	4	0.5505794325880515
1110	5	0.6537219533010159
1110	6	0.7656957029026614
1110	7	0.7366939683657137
1110	8	1.10520288043773
1110	9	0.7512983854161561
1110	10	0.7840542871610126
1110	11	0.3863738714428777
1110	12	0.5933847312950462
1110	13	0.6155107250811866
1110	14	0.4294925172631565
1110	15	1.123883526533291
1110	16	0.8366055224136204
1110	17	0.27460452618098685
1110	18	0.383320883738719
1110	19	-1.8131332174789705
1110	20	0.013875201757912237
1110	21	0.11508488939785622
1110	22	0.12493609920862525
1110	23	0.2522649849218368
1110	24	-1.1530276059377869
1110	25	-0.7271724924601415
1111	1	0.6730287813475755
1111	2	0.18399366777178727
1111	3	0.8701431377857141
1111	4	0.8078295079727909
1111	5	0.8133889049081588
1111	6	0.8056005106508337
1111	7	0.8217812071818003
1111	8	1.0628484144798165
1111	9	0.6012212025092651
1111	10	0.4399561514463528
1111	11	0.17891161222449625
1111	12	0.3707265685801957
1111	13	0.40392512813087933
1111	14	0.2926297949473309
1111	15	-0.5155865395009727
1111	16	-0.28605236365998365
1111	17	-0.056769495546411974
1111	18	0.39281590281312617
1111	19	1.0222216717400414
1111	20	0.13292153639976334
1111	21	0.47357044457532993
1111	22	0.707515585153871
1111	23	0.4582465218852576
1111	24	1.0886655491279313
1111	25	0.7802207447680232
1112	1	-0.03196801037425345
1112	2	0.14608183721219348
1112	3	-1.2464486690569885
1112	4	-0.24284639636907457
1112	5	-0.1982522715146473
1112	6	0.8344526780660999
1112	7	0.6066593873706196
1112	8	1.060400241032447
1112	9	-0.38480248189362953
1112	10	0.26787534615499453
1112	11	0.1263872252492959
1112	12	0.20875522378369737
1112	13	-0.1690618465304894
1112	14	0.14082544306013745
1112	15	0.37413262109432566
1112	16	0.30885649145850635
1112	17	-0.2882061543886749
1112	18	0.05355131597708862
1112	19	0.7445587331738324
1112	20	0.3870191980116999
1112	21	0.4968731029789879
1112	22	0.30857431694993664
1112	23	0.19690857512913595
1112	24	1.4340901654205709
1112	25	0.5167710199760194
1113	1	-0.5450494009726476
1113	2	-0.040050091185779735
1113	3	-4.564961328836247
1113	4	-1.280420791160708
1113	5	0.17898986739484712
1113	6	0.6051843534972647
1113	7	0.8648044935741837
1113	8	1.142834082369312
1113	9	0.6209817642228117
1113	10	0.6367735025041696
1113	11	0.3737698032228849
1113	12	0.3739079026498615
1113	13	0.07697484346098094
1113	14	-0.4473962395286222
1113	15	0.29971331717791827
1113	16	0.650696351052126
1113	17	0.3832742897086092
1113	18	0.26063242550125665
1113	19	1.2347136181458396
1113	20	0.5501141520756718
1113	21	0.6856883097483006
1113	22	0.6211974911930795
1113	23	0.4720710915932216
1113	24	1.9475297622424677
1113	25	1.290380764754083
1114	1	0.6927139475859256
1114	2	0.5866849539874721
1114	3	0.8407194468405415
1114	4	0.7558526666759704
1114	5	0.9643548355126157
1114	6	1.2309060395763822
1114	7	1.325213792100179
1114	8	1.197018645986283
1114	9	0.5115584323266376
1114	10	0.44425342820413505
1114	11	0.2611546819342365
1114	12	-0.2972418352577435
1114	13	0.2563011509243722
1114	14	0.3573930654159625
1114	15	-2.1799303538661405
1114	16	-0.11293877483974768
1114	17	0.08701036250448624
1114	18	0.3605504101938948
1114	19	0.6288694601966753
1114	20	0.6649064069779484
1114	21	0.45170609377898074
1114	22	0.44385640800751247
1114	23	-0.026550208398020914
1114	24	1.172035806414037
1114	25	1.0105510194553773
1115	1	-3.7598349206705493
1115	2	-1.1691577842927323
1115	3	-0.10178388376729686
1115	4	0.469069592861473
1115	5	0.6753217363331174
1115	6	1.1958161214539658
1115	7	1.0849663887806429
1115	8	1.2807125603075988
1115	9	0.7142211944400003
1115	10	-0.5257555175200537
1115	11	0.062086787932777554
1115	12	-0.19507224846532267
1115	13	0.2560538006558346
1115	14	0.36961866742871763
1115	15	0.738635704239293
1115	16	0.5581563237124243
1115	17	0.18914745556190127
1115	18	0.23907821853125455
1115	19	-1.9202706826298694
1115	20	-0.4558343440782622
1115	21	-0.19588011906294156
1115	22	0.09700899659770101
1115	23	-0.13455407846817735
1115	24	-2.0600026184223204
1115	25	-1.034097361859267
1116	1	0.11147709046420218
1116	2	-0.5852886175996055
1116	3	-0.14858462784799187
1116	4	-0.6428289507302196
1116	5	0.3713884548631583
1116	6	0.8819474310571565
1116	7	0.6374502740884935
1116	8	-1.8212757122185685
1116	9	-0.6069487216260043
1116	10	-0.51532330507073
1116	11	-0.0368287118079067
1116	12	0.06148939486282501
1116	13	-0.00591049828890533
1116	14	-0.07483195122728148
1116	15	-0.6017005080406861
1116	16	-0.35981420412974074
1116	17	-0.1593235009469538
1116	18	-0.2956431216607456
1116	19	0.41189903233324543
1116	20	0.013707829314768105
1116	21	0.021010009960441778
1116	22	-0.07288656220372758
1116	23	0.22246044327739867
1116	24	0.989460092868022
1116	25	0.38934594300213377
1201	1	0.441846082785748
1201	2	0.11007350203258781
1201	3	0.5062937966472205
1201	4	0.4180713715752091
1201	5	0.3947378244716546
1201	6	0.6864905157097283
1201	7	0.7370687213346798
1201	8	0.9922255675235832
1201	9	0.4927348976744028
1201	10	0.5301430898482238
1201	11	0.6484317394196992
1201	12	0.6467166675900984
1201	13	0.5585979859276122
1201	14	0.6115482743865215
1201	15	1.3126397137812873
1201	16	0.6474080444246226
1201	17	0.42681618053944703
1201	18	0.45673269595229726
1201	19	-0.08861641936833564
1201	20	0.6207440412779874
1201	21	0.8142783292324083
1201	22	0.7210571765392828
1201	23	0.731999262122784
1201	24	-0.2889963958986854
1201	25	-0.035623248390415085
1202	1	0.03985412933027277
1202	2	-0.011838986791659067
1202	3	-0.291805323970884
1202	4	-0.454468294019474
1202	5	0.04210268122180594
1202	6	0.43681578025822887
1202	7	0.2727544123633834
1202	8	0.7136906924918947
1202	9	0.6185388849173634
1202	10	0.5922736226815886
1202	11	0.39844841965295785
1202	12	0.7100164847747408
1202	13	0.3084161713851188
1202	14	0.40177599293093635
1202	15	1.0531019564052855
1202	16	0.8786277698012626
1202	17	0.6260865871368111
1202	18	0.6104310372699047
1202	19	0.16620083199610747
1202	20	0.6625747302383402
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2211	5	0.8010618443875401
2211	6	-2.0775219075512545
2211	7	0.35275731803792354
2211	8	0.6546101568792224
2211	9	0.9072585115381813
2211	10	0.7748740803253327
2211	11	0.7692745392456786
2211	12	0.8867828927556616
2211	13	0.664673057228427
2211	14	0.6558272901935496
2211	15	1.2913824024263292
2211	16	0.4392579985020646
2211	17	0.6957713214491932
2211	18	0.6594022468780025
2211	19	1.0453868193806386
2211	20	0.760880358772063
2211	21	0.3824370762716143
2211	22	-2.6369454337957094
2211	23	-0.859718966716585
2211	24	1.2204902382193552
2211	25	0.8054786513533827
2212	1	-0.8553598285785995
2212	2	-0.35403358487398506
2212	3	-3.171960993136125
2212	4	-1.940251420946808
2212	5	-0.5850629832059262
2212	6	0.1671558633244139
2212	7	0.18678905136844648
2212	8	-0.007703354528565853
2212	9	-0.004876012935646656
2212	10	0.014534952728702422
2212	11	-0.1329498998408596
2212	12	-0.041876337179374445
2212	13	-0.03392084247571603
2212	14	-0.5046989840354925
2212	15	-1.4721942829462265
2212	16	-0.46253213405355353
2212	17	-0.277593843482002
2212	18	-0.4223013402307316
2212	19	0.4230084515308974
2212	20	-0.7972087977783318
2212	21	-0.7318947760325756
2212	22	-0.06811837728847792
2212	23	-0.1063984958756592
2212	24	1.096766836204175
2212	25	0.4730801737951893
2213	1	-0.3805553770682657
2213	2	-0.5037951111061005
2213	3	0.10091277474941052
2213	4	-7.19393154813282
2213	5	-1.5226388178641166
2213	6	-3.193429192319467
2213	7	-1.2878042713660527
2213	8	-2.8243977901406474
2213	9	-0.8492461242234057
2213	10	-0.18565837000579677
2213	11	-0.2618923481715427
2213	12	0.049716667590097074
2213	13	-0.16074945932786733
2213	14	-0.1278137694091015
2213	15	-2.7898070015471816
2213	16	-0.7761817365972732
2213	17	-0.5372962282196809
2213	18	-0.18586000477763065
2213	19	0.4343470842812991
2213	20	-1.2502428200358864
2213	21	-0.29575232770189785
2213	22	-0.2100566920738558
2213	23	-0.2141759203589686
2213	24	-0.4763905564826274
2213	25	-0.18886704401085552
2214	1	0.60583003133776
2214	2	0.482564920614756
2214	3	0.37063648608961586
2214	4	0.6108291180051637
2214	5	0.7219264477980545
2214	6	0.5178888092665801
2214	7	0.9235778063500462
2214	8	-2.223345292123202
2214	9	-1.0496432693463262
2214	10	-0.4056945317821601
2214	11	0.11769781836295579
2214	12	-1.0821691373266376
2214	13	-0.2521397844666424
2214	14	-0.10812082729966477
2214	15	-1.3133870174076065
2214	16	-0.22232733532844406
2214	17	-0.1081961489175427
2214	18	0.009037378251711914
2214	19	0.8102487496262611
2214	20	-0.9588981729304109
2214	21	-0.30213012310237275
2214	22	0.1506830382988653
2214	23	-0.24991898142161517
2214	24	-1.4041907150869122
2214	25	-0.5933054262471771
2215	1	0.31825610470054855
2215	2	0.009160668568064523
2215	3	0.05681533487353718
2215	4	0.44358342471434753
2215	5	0.4572240215462813
2215	6	1.027383841039729
2215	7	0.45438905679531416
2215	8	-0.7547059359901418
2215	9	-0.38527343221409893
2215	10	-0.5960651970034689
2215	11	-0.8981244660924119
2215	12	-0.8766541625107536
2215	13	-0.6163870078150637
2215	14	-0.5976297507900199
2215	15	-0.48514105895602455
2215	16	-0.30844736887035396
2215	17	-0.5900000296161139
2215	18	-1.547602130456447
2215	19	-0.403831348612421
2215	20	-4.0725124864439515
2215	21	-2.411250853691037
2215	22	-1.5216570023919331
2215	23	-1.532661568147951
2215	24	-1.2854239156757998
2215	25	-1.0053474163925458
2216	1	-0.7943465163087708
2216	2	-1.2496811870554652
2216	3	-0.47858216195945147
2216	4	-0.8802631937024401
2216	5	-0.7375729950793044
2216	6	-2.00962539485111
2216	7	-10.145346043517954
2216	8	-11.367552220520395
2216	9	-6.101318276122139
2216	10	-3.7361128003855413
2216	11	-2.5117999431082474
2216	12	-2.281289661523825
2216	13	-1.9416330036316651
2216	14	-2.0108935162445434
2216	15	-2.968624723066167
2216	16	-2.2569969264706913
2216	17	-2.496564582650059
2216	18	-2.189835954144719
2216	19	-1.5345706372376924
2216	20	-2.4299466175042355
2216	21	-5.575354859347463
2216	22	-3.8570288439725893
2216	23	-3.4900683254222606
2216	24	-3.727137391925666
2216	25	-3.116654385783008
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36887.0
3	2.0
4	287.0
5	1749.0
6	2225.0
7	1790.0
8	1398.0
9	1349.0
10	1280.0
11	1282.0
12	1209.0
13	1212.0
14	1201.0
15	1298.0
16	1310.0
17	1503.0
18	1730.0
19	2095.0
20	2696.0
21	3592.0
22	4373.0
23	5401.0
24	6419.0
25	7465.0
26	8350.0
27	9080.0
28	10083.0
29	12217.0
30	14574.0
31	17915.0
32	22943.0
33	30766.0
34	41847.0
35	58983.0
36	86638.0
37	141913.0
38	213419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78060737722031	18.227923706528603	12.010116787047975	25.981352129203106
2	14.446357416537056	19.782097221818795	42.175225309677025	23.59632005196712
3	19.329935700233698	23.773353226211068	29.025601077384174	27.87110999617106
4	11.459122152007282	16.663676301798176	38.83151492763757	33.04568661855697
5	12.474615607774876	38.530606324340006	36.359944088403616	12.634833979481499
6	31.559632301648733	37.61180685682788	18.663289109487973	12.165271732035409
7	27.80303390933181	30.946959479115428	22.912997254883294	18.33700935666947
8	24.266137051219665	38.188244441153444	20.74901622696596	16.796602280660927
9	25.861796143773784	15.464464013038699	20.083918149475576	38.58982169371194
10	13.514247806051527	29.9342408951468	35.68625020112843	20.86526109767324
11	34.79044008570958	22.531201582330638	24.456634250865335	18.221724081094447
12	22.702907981818683	24.905025443027792	32.660830396077856	19.731236179075665
13	29.125762967983366	22.090172959188383	25.715498815248676	23.068565257579575
14	19.827498678087636	22.535012546629552	28.091659623060483	29.545829152222332
15	22.607297934146384	32.05930016522082	22.99760410032451	22.33579780030829
16	21.298345091316676	28.728423551130746	28.767851256016353	21.20538010153623
17	21.188381755870243	29.144112484984024	28.38259979772113	21.284905961424602
18	20.88343457971653	28.332460860701943	30.266449185831394	20.51765537375013
19	23.201753344885155	27.355397838912758	27.355661579118106	22.08718723708398
20	22.72750648436171	28.22975277011141	28.546619494885693	20.496121250641185
21	22.32806158374012	27.421405043896087	27.395824267981045	22.85470910438275
22	22.04013602840826	28.93267279471393	27.82754087379396	21.199650303083846
23	21.588673393498567	28.424644889735163	27.977361555786462	22.009320160979804
24	22.65796729560412	27.467235606940683	28.740758126980403	21.134038970474794
25	22.115254022735396	27.824156059585142	28.468690374312512	21.591899543366956
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	179.0
1	179.0
2	238.5
3	298.0
4	298.0
5	298.0
6	505.5
7	713.0
8	713.0
9	713.0
10	1055.5
11	1398.0
12	1398.0
13	1398.0
14	2392.0
15	3386.0
16	3386.0
17	3386.0
18	6059.5
19	8733.0
20	8733.0
21	8733.0
22	13813.0
23	18893.0
24	18893.0
25	18893.0
26	27736.5
27	36580.0
28	36580.0
29	36580.0
30	48576.5
31	60573.0
32	60573.0
33	60573.0
34	68701.0
35	76829.0
36	76829.0
37	76829.0
38	83281.0
39	89733.0
40	89733.0
41	89733.0
42	93818.0
43	97903.0
44	97903.0
45	97903.0
46	99995.0
47	102087.0
48	102087.0
49	102087.0
50	96605.5
51	91124.0
52	91124.0
53	91124.0
54	79635.5
55	68147.0
56	68147.0
57	68147.0
58	57915.5
59	47684.0
60	47684.0
61	47684.0
62	38174.0
63	28664.0
64	28664.0
65	28664.0
66	21873.0
67	15082.0
68	15082.0
69	15082.0
70	10805.0
71	6528.0
72	6528.0
73	6528.0
74	4585.0
75	2642.0
76	2642.0
77	2642.0
78	1778.5
79	915.0
80	915.0
81	915.0
82	599.0
83	283.0
84	283.0
85	283.0
86	179.0
87	75.0
88	75.0
89	75.0
90	51.0
91	27.0
92	27.0
93	27.0
94	16.0
95	5.0
96	5.0
97	5.0
98	2.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.22663718669287694
2	0.14225801305504027
3	0.1438401225607497
4	0.06473464727527782
5	0.018589786692085893
6	0.018721629150895012
7	0.29387684068552805
8	0.29123999150934565
9	0.016216622433521736
10	0.03414719683156203
11	0.013975300633766698
12	0.015821095057094378
13	0.012393191128057262
14	0.014502670469003179
15	0.01344793079853022
16	0.017271362103994693
17	0.016084779974712617
18	0.013184245880911981
19	0.02096295095065005
20	0.015557410139476136
21	0.01305240342210286
22	0.014502670469003179
23	0.016480307351139974
24	0.014766355386621417
25	0.01371161571614846
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	758481.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.33339932148493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.97816829107661	81.13454730575229
2	4.631369880797826	7.996838106488335
3	0.6916451186885662	1.7913622266148743
4	0.22663433502861022	0.7826445018393682
5	0.11899717628169343	0.5136715369028287
6	0.07443114807231724	0.3855536417090362
7	0.05061145796118559	0.30586214472839074
8	0.034375251802809294	0.23741858725348236
9	0.02919378806912788	0.22683590667709375
>10	0.13737677857331004	2.2143646391099567
>50	0.013751843579798818	0.8186547692287204
>100	0.01145874724133413	2.069162558455801
>500	0.0013750496493357108	0.7817891499044552
>1k	6.11133177482538E-4	0.7412949253354206
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	1764	0.23257009733928732	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1378	0.1816789082389671	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1313	0.17310914841637431	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1165	0.15359646451262457	No Hit
CTTTAGGACGTGAAATATGGCGAGG	939	0.12380006882176349	No Hit
GTATCAACGCAGAGTACTTTTTTTT	818	0.10784713130586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	1.318424588091198E-4
8	0.0	0.0	0.0	0.0	1.318424588091198E-4
9	0.0	0.0	0.0	0.0	1.318424588091198E-4
10	0.0	0.0	0.0	1.318424588091198E-4	1.318424588091198E-4
11	0.0	0.0	0.0	1.318424588091198E-4	1.318424588091198E-4
12	0.0	0.0	0.0	1.318424588091198E-4	1.318424588091198E-4
13	0.0	0.0	0.0	1.318424588091198E-4	1.318424588091198E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTCTAT	60	2.45124E-5	14.318594	1
TAGGACC	280	0.0	13.940666	4
AGAACTG	130	1.9099389E-10	12.444881	5
CTATACT	115	5.136826E-9	12.417984	4
CAGGACT	215	0.0	11.955919	4
AGGACGT	665	0.0	11.734846	5
GTAGGAC	765	0.0	11.713843	3
TGTAGGA	775	0.0	11.687254	2
AGGACTG	180	0.0	11.63149	5
GGACGTG	655	0.0	11.623419	6
ATAGGAC	115	6.719165E-8	11.605483	3
GTCCTAC	875	0.0	11.454875	1
TAGGACT	85	5.199077E-5	11.200534	4
CTGTAGG	785	0.0	11.18735	1
GACGTGA	350	0.0	11.147367	7
CCTTAAG	60	0.005664732	11.136684	1
GCTATAA	60	0.005664732	11.136684	1
GTCCTAA	390	0.0	11.014302	1
TAGGACG	720	0.0	10.974967	4
GTCTAAT	70	0.0014313454	10.909405	1
>>END_MODULE