FastQCFastQC Report
Thu 26 May 2016
SRR1926742_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926742_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences758481
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18180.2396895901149798No Hit
CTGTAGGACGTGGAATATGGCAAGA16810.2216271732581304No Hit
GTATCAACGCAGAGTACTTTTTTTT13930.18365654512110388No Hit
GTCCTAAAGTGTGTATTTCTCATTT12840.16928571711090984No Hit
CTTTAGGACGTGAAATATGGCGAGG11900.15689252598285258No Hit
GTCCTACAGTGTGCATTTCTCATTT10650.14041221863171258No Hit
CTGTAGGACCTGGAATATGGCGAGA8800.11602136375202543No Hit
GTACATGGGAAGCAGTGGTATCAAC8500.11206608998775183No Hit
GGTATCAACGCAGAGTACTTTTTTT8070.10639686425895968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACT653.2957905E-614.6453524
CTTGGAC801.2516102E-714.2811073
CTAGGAC1051.3224053E-913.6010543
GGCGAGG3050.013.3784319
GATATAC2150.012.8580871
TTGGACA905.260372E-712.6926384
CTCGAAC604.126585E-412.6524718
TCGAACT604.126585E-412.6524719
GTCCTAC9050.012.5347371
TAGGACC3800.012.525634
GTTCTAT1301.8553692E-1012.4658641
GTAGGAC8850.012.0488443
TCCTACA9400.011.9658132
GCTAGAC657.869769E-411.7178323
CTGTAGG8400.011.688931
GTCCAAG907.1666163E-611.6511011
TGTAGGA9250.011.6446052
AGGACGT7950.011.6149625
CCTACAG9200.011.5904643
GCCATAT2150.011.527941
Produced by FastQC (version 0.11.5)