##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926742_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 758481 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.011200016875836 31.0 30.0 33.0 26.0 34.0 2 30.314696083356075 31.0 30.0 34.0 26.0 34.0 3 30.388326141327205 31.0 30.0 34.0 26.0 34.0 4 33.96555220236235 37.0 35.0 37.0 30.0 37.0 5 33.95539374091111 37.0 35.0 37.0 30.0 37.0 6 33.90443399373221 37.0 35.0 37.0 30.0 37.0 7 33.885167855226435 37.0 35.0 37.0 30.0 37.0 8 33.90666740498444 37.0 35.0 37.0 30.0 37.0 9 35.458827577750796 39.0 35.0 39.0 30.0 39.0 10 35.352400389726306 39.0 35.0 39.0 30.0 39.0 11 35.3989658277531 39.0 35.0 39.0 30.0 39.0 12 35.2870276249504 39.0 35.0 39.0 30.0 39.0 13 35.31841535911908 39.0 35.0 39.0 30.0 39.0 14 36.42806477683686 40.0 37.0 41.0 30.0 41.0 15 36.41709943953771 40.0 37.0 41.0 30.0 41.0 16 36.39351018680758 40.0 37.0 41.0 30.0 41.0 17 36.367978894659196 40.0 37.0 41.0 30.0 41.0 18 36.343025072480394 40.0 37.0 41.0 30.0 41.0 19 36.40979273047051 40.0 37.0 41.0 30.0 41.0 20 36.36366896468073 40.0 37.0 41.0 29.0 41.0 21 36.31778119689221 40.0 37.0 41.0 29.0 41.0 22 36.23084032427971 40.0 37.0 41.0 29.0 41.0 23 36.15815030303989 40.0 37.0 41.0 29.0 41.0 24 36.09117169711568 40.0 37.0 41.0 29.0 41.0 25 35.97891179871348 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3902601401286958 1101 2 -0.3398928206453249 1101 3 -0.021096301662065997 1101 4 -0.11955492462369932 1101 5 -0.061017832790305704 1101 6 -0.2442957315520431 1101 7 -0.2962522806821468 1101 8 -0.29576866501587773 1101 9 -0.2606869756324244 1101 10 -0.23132299280090507 1101 11 -0.25649278916794316 1101 12 -0.08304617380249368 1101 13 -0.16913411492148356 1101 14 -0.22746281176841165 1101 15 -0.14516769960889064 1101 16 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0.03589593178644179 2211 12 0.08518766794935573 2211 13 -0.02945843363298195 2211 14 -0.2510108192446978 2211 15 -0.06761849223413208 2211 16 -0.2259396684588566 2211 17 -0.08019913308124416 2211 18 -0.2699331171503232 2211 19 -0.18655425490534583 2211 20 -0.2580154793601679 2211 21 -0.258731092070569 2211 22 -0.18822314867205137 2211 23 -0.13744275985732202 2211 24 -0.2612296712762898 2211 25 -0.19206903280321797 2212 1 -0.20475607693453313 2212 2 -0.06161110584998042 2212 3 -0.1083164896713491 2212 4 -0.10375499885247308 2212 5 -0.03494636267292606 2212 6 0.017295589221490104 2212 7 -0.03551680653203704 2212 8 -0.041478352978074895 2212 9 0.051692887321323155 2212 10 -0.10104873411918902 2212 11 0.0483907990728909 2212 12 -0.01938808037395745 2212 13 -0.06539664102619014 2212 14 0.04838431020496614 2212 15 -0.025338093409125406 2212 16 0.07761690720523973 2212 17 0.035734389065758876 2212 18 0.033805077608519696 2212 19 0.029423750759065115 2212 20 0.11483009703040636 2212 21 0.003934865667460485 2212 22 0.029757931356165557 2212 23 0.09501125629361695 2212 24 0.09070996801239772 2212 25 0.03519041402186929 2213 1 0.12584996440092056 2213 2 0.3198744267414355 2213 3 0.34149463910448574 2213 4 0.34324786283274733 2213 5 0.17721770727938235 2213 6 0.31921228238523724 2213 7 0.27615886914364296 2213 8 0.1854787217437064 2213 9 0.3828572752386563 2213 10 0.15403171103184832 2213 11 0.3006667927135922 2213 12 0.29166184013478613 2213 13 0.35753626835377617 2213 14 0.22337063770893906 2213 15 0.06655634220295781 2213 16 0.11314377524975328 2213 17 0.03268828413730063 2213 18 0.13531986298212928 2213 19 0.2514523676112148 2213 20 0.23957922262658826 2213 21 0.34674440461817113 2213 22 0.2665808248378809 2213 23 0.37190028650029205 2213 24 0.3199716532270216 2213 25 0.37777864931598515 2214 1 0.5475463958045701 2214 2 0.41127046163437697 2214 3 0.4096462647983792 2214 4 0.4247679262744555 2214 5 0.45696330601055024 2214 6 0.3529233053828591 2214 7 0.07671715621739139 2214 8 0.16033677091103726 2214 9 0.17660699768116217 2214 10 0.03004249612304477 2214 11 6.077919205722537E-4 2214 12 0.1957747663837992 2214 13 0.1585329376638498 2214 14 0.07027182724105785 2214 15 0.12924119549399649 2214 16 0.17493790689130861 2214 17 0.22193808588194486 2214 18 0.0622863974785588 2214 19 0.20204396158425197 2214 20 0.17710118931969276 2214 21 0.03570174006623006 2214 22 0.12193816028593574 2214 23 0.17527157912519442 2214 24 0.015696950610049498 2214 25 0.08825287612010868 2215 1 0.25199488294242656 2215 2 0.2159718666173056 2215 3 0.2518288516723679 2215 4 0.259185178581383 2215 5 0.3879243015384972 2215 6 0.24874711558927487 2215 7 0.08042263950826367 2215 8 0.09199850452105096 2215 9 0.04135347384222143 2215 10 0.22077368155162702 2215 11 0.1717718353823301 2215 12 0.08687797667474229 2215 13 0.1569025988425281 2215 14 0.010785377817555286 2215 15 0.022673021799541004 2215 16 0.039441370284663435 2215 17 0.2115525199790369 2215 18 0.06932700029787497 2215 19 -0.033183784444645426 2215 20 -0.011045447660450236 2215 21 -0.05637056822899211 2215 22 0.017453128949597385 2215 23 0.11659291644598824 2215 24 0.21785404267620834 2215 25 0.16404086809022544 2216 1 -0.13410193433325546 2216 2 -0.08249139604337685 2216 3 -1.9822863735537446 2216 4 -1.4606103650153557 2216 5 -0.5605822927206177 2216 6 -0.2030737935641298 2216 7 -0.2198094852867385 2216 8 -0.15383773395249278 2216 9 -0.21647690197653446 2216 10 -0.18046955479093896 2216 11 -0.08729649842565124 2216 12 -0.10590651429559017 2216 13 -0.2277776556968547 2216 14 -0.104147083810048 2216 15 -0.18131201222742277 2216 16 -0.07472204753505451 2216 17 -0.27391298168548417 2216 18 -0.019335833220402776 2216 19 -0.22188307542676 2216 20 -0.0678853343354362 2216 21 -0.19929610171094225 2216 22 -0.07587613718743569 2216 23 -0.08662123248704745 2216 24 -0.2757321442413314 2216 25 -0.007670717772619184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17042.0 3 21.0 4 269.0 5 1799.0 6 2514.0 7 1910.0 8 1460.0 9 1249.0 10 1107.0 11 1046.0 12 931.0 13 868.0 14 869.0 15 918.0 16 896.0 17 989.0 18 1173.0 19 1352.0 20 1720.0 21 2257.0 22 2772.0 23 3348.0 24 4137.0 25 4900.0 26 5782.0 27 6406.0 28 7510.0 29 8964.0 30 11613.0 31 15145.0 32 19774.0 33 26117.0 34 35513.0 35 49900.0 36 73478.0 37 128166.0 38 314566.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.4461533181805 18.890094428033272 9.848682838490314 28.81506941529592 2 14.156695288317483 20.834361820785787 40.18374175835055 24.825201132546184 3 18.890292565466567 24.130519064360183 29.06573162465747 27.91345674551578 4 11.859492854797944 17.025739603233305 38.12211512219818 32.99265241977057 5 12.75373440345138 38.69170289324795 35.6764910254331 12.87807167786757 6 32.02386622215167 38.18807218076943 17.58678626236969 12.201275334709214 7 28.45130788555754 31.546186460235926 21.3679553202292 18.63455033397733 8 24.82795904508864 38.81183288391933 19.61115249968684 16.749055571305192 9 26.12903651155738 15.715001516370206 19.453711151254634 38.70225082081779 10 13.84955758046419 30.29495954211457 34.7530401098039 21.102442767617344 11 34.836088809895195 22.702322827845453 24.14675599166171 18.314832370597646 12 23.081973697221397 25.114077589900784 32.00421899205643 19.799729720821386 13 29.25609548684129 22.153121213599466 25.578037921027963 23.01274537853128 14 20.05613851896092 22.78751873810946 27.630224092627238 29.52611865030238 15 22.503905966735008 32.05410999993408 22.971962740043907 22.47002129328701 16 21.391400880453226 28.897139383999164 28.321509137437985 21.38995059810962 17 21.013266040676196 29.335854171116367 28.111032884276142 21.539846903931295 18 20.942444961857625 28.459775813014026 30.005642909315284 20.592136315813068 19 22.98289862448037 27.451326803581388 27.423244211755925 22.142530360182313 20 22.786806139696285 28.279677352157094 28.36656233437138 20.566954173775237 21 22.253195001925178 27.411085852325773 27.223974513826988 23.111744631922065 22 22.22586986358261 29.074426386422335 27.35454151125737 21.34516223873769 23 21.56929441871319 28.60032090454474 27.861212080460817 21.969172596281254 24 22.565423696634976 27.501222842029904 28.64416813122051 21.28918533011461 25 22.09977822958117 28.08586549795502 28.19477296879656 21.619583303667255 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 245.0 1 245.0 2 253.5 3 262.0 4 262.0 5 262.0 6 397.5 7 533.0 8 533.0 9 533.0 10 911.0 11 1289.0 12 1289.0 13 1289.0 14 2144.5 15 3000.0 16 3000.0 17 3000.0 18 5389.5 19 7779.0 20 7779.0 21 7779.0 22 12772.5 23 17766.0 24 17766.0 25 17766.0 26 26095.0 27 34424.0 28 34424.0 29 34424.0 30 46532.5 31 58641.0 32 58641.0 33 58641.0 34 67351.5 35 76062.0 36 76062.0 37 76062.0 38 82173.0 39 88284.0 40 88284.0 41 88284.0 42 93210.5 43 98137.0 44 98137.0 45 98137.0 46 100667.0 47 103197.0 48 103197.0 49 103197.0 50 98692.0 51 94187.0 52 94187.0 53 94187.0 54 82106.0 55 70025.0 56 70025.0 57 70025.0 58 59336.5 59 48648.0 60 48648.0 61 48648.0 62 38967.0 63 29286.0 64 29286.0 65 29286.0 66 22456.0 67 15626.0 68 15626.0 69 15626.0 70 11301.5 71 6977.0 72 6977.0 73 6977.0 74 4852.0 75 2727.0 76 2727.0 77 2727.0 78 1854.0 79 981.0 80 981.0 81 981.0 82 632.5 83 284.0 84 284.0 85 284.0 86 190.0 87 96.0 88 96.0 89 96.0 90 59.0 91 22.0 92 22.0 93 22.0 94 12.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11430741178750686 2 0.11813084309297134 3 0.02043558111541357 4 0.0 5 0.007910547528547189 6 0.34292223536252064 7 0.025841121926587482 8 0.011338451457584303 9 0.011997663751629903 10 0.005010013434746552 11 0.00685580785807423 12 7.910547528547189E-4 13 0.001318424588091198 14 0.0 15 0.003427903929037115 16 0.0010547396704729585 17 3.9552737642735945E-4 18 9.228972116638386E-4 19 5.273698352364792E-4 20 9.228972116638386E-4 21 0.01437082801019406 22 0.0 23 0.0 24 2.636849176182396E-4 25 0.005141855893555673 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 758481.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.94532434038334 #Duplication Level Percentage of deduplicated Percentage of total 1 92.47934475545617 77.63208590282898 2 5.820534731620651 9.772133517607234 3 0.8719104917718737 2.1957842708271924 4 0.2918851821198414 0.9800958513280777 5 0.14513631732162122 0.6091757615566145 6 0.08577178290073656 0.4320084080913166 7 0.054273574871708176 0.31892089920023353 8 0.03975489858133364 0.2669790284423278 9 0.029634611744357685 0.22389183851232952 >10 0.15046212452181537 2.322379662014024 >50 0.01651117883944925 0.9735110600696835 >100 0.012421783742066128 2.2947114193625313 >500 0.0014151399049997112 0.8654830428813629 >1k 9.434266033331407E-4 1.112839337278141 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1818 0.2396895901149798 No Hit CTGTAGGACGTGGAATATGGCAAGA 1681 0.2216271732581304 No Hit GTATCAACGCAGAGTACTTTTTTTT 1393 0.18365654512110388 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1284 0.16928571711090984 No Hit CTTTAGGACGTGAAATATGGCGAGG 1190 0.15689252598285258 No Hit GTCCTACAGTGTGCATTTCTCATTT 1065 0.14041221863171258 No Hit CTGTAGGACCTGGAATATGGCGAGA 880 0.11602136375202543 No Hit GTACATGGGAAGCAGTGGTATCAAC 850 0.11206608998775183 No Hit GGTATCAACGCAGAGTACTTTTTTT 807 0.10639686425895968 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACT 65 3.2957905E-6 14.645352 4 CTTGGAC 80 1.2516102E-7 14.281107 3 CTAGGAC 105 1.3224053E-9 13.601054 3 GGCGAGG 305 0.0 13.37843 19 GATATAC 215 0.0 12.858087 1 TTGGACA 90 5.260372E-7 12.692638 4 CTCGAAC 60 4.126585E-4 12.65247 18 TCGAACT 60 4.126585E-4 12.65247 19 GTCCTAC 905 0.0 12.534737 1 TAGGACC 380 0.0 12.52563 4 GTTCTAT 130 1.8553692E-10 12.465864 1 GTAGGAC 885 0.0 12.048844 3 TCCTACA 940 0.0 11.965813 2 GCTAGAC 65 7.869769E-4 11.717832 3 CTGTAGG 840 0.0 11.68893 1 GTCCAAG 90 7.1666163E-6 11.651101 1 TGTAGGA 925 0.0 11.644605 2 AGGACGT 795 0.0 11.614962 5 CCTACAG 920 0.0 11.590464 3 GCCATAT 215 0.0 11.52794 1 >>END_MODULE