FastQCFastQC Report
Thu 26 May 2016
SRR1926741_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926741_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861930
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT26090.30269279407840544No Hit
GTCCTAAAGTGTGTATTTCTCATTT20690.24004269488241506No Hit
CTGTAGGACGTGGAATATGGCAAGA20080.2329655540473124No Hit
GTCCTACAGTGTGCATTTCTCATTT19080.2213636838258327No Hit
CTTTAGGACGTGAAATATGGCGAGG14740.17101156706461082No Hit
CTGTAGGACCTGGAATATGGCGAGA13400.15546506096782803No Hit
GTATCAACGCAGAGTACTTTTTTTT9530.11056582321070157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT250.00596594819.0351226
TAGGACC6100.014.05048754
TCCAACG1152.5465852E-1114.01718618
TTACACC500.001471406513.3323524
CTAGTAC603.9626312E-412.7137773
TGTAGGA13350.012.214512
CCGGTTT550.003111226512.06757212
GGCGAGG4750.011.97753419
GTAGGAC12950.011.928373
AGGACCT9850.011.7882485
CCAACGA907.606668E-611.58937419
CTGTAGG13950.011.4894461
TCTAGTA752.0004406E-411.4430682
TAGTACC752.0255278E-411.4277294
GGACCTG9250.011.4210736
GTCCTAA6750.011.3070741
GTCCTAC14000.011.2439761
GACAGTA600.005791862511.1038217
TGGCGAG11900.011.07586418
TGCCGGT600.00595195811.06322510
Produced by FastQC (version 0.11.5)