##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926741_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 861930 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.056001067372062 31.0 30.0 33.0 25.0 34.0 2 29.123914935087537 31.0 30.0 33.0 25.0 34.0 3 28.846663882217815 31.0 30.0 33.0 22.0 34.0 4 32.72100750641003 35.0 33.0 37.0 26.0 37.0 5 32.66024039075099 35.0 33.0 37.0 25.0 37.0 6 32.226548559627815 35.0 33.0 37.0 21.0 37.0 7 32.23349807989048 35.0 33.0 37.0 22.0 37.0 8 32.0771292332324 35.0 33.0 37.0 18.0 37.0 9 34.25630735674591 38.0 35.0 39.0 26.0 39.0 10 34.380403281008896 39.0 35.0 39.0 27.0 39.0 11 34.540134349657166 39.0 35.0 39.0 27.0 39.0 12 34.40398756279512 39.0 35.0 39.0 27.0 39.0 13 34.52133699952432 39.0 35.0 39.0 27.0 39.0 14 35.62087872565058 40.0 36.0 41.0 27.0 41.0 15 35.22530715951412 40.0 36.0 41.0 25.0 41.0 16 35.31319480700289 39.0 36.0 41.0 26.0 41.0 17 35.548596753796716 40.0 36.0 41.0 27.0 41.0 18 35.60220783590315 40.0 36.0 41.0 27.0 41.0 19 35.00569767846577 40.0 35.0 41.0 22.0 41.0 20 35.283938370865386 40.0 36.0 41.0 25.0 41.0 21 35.27357326000951 40.0 36.0 41.0 25.0 41.0 22 35.30749828872414 40.0 36.0 41.0 25.0 41.0 23 35.32660192823083 40.0 36.0 41.0 26.0 41.0 24 34.07607810379033 39.0 34.0 41.0 11.0 41.0 25 34.65536876544499 39.0 35.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.08506654831971616 1101 2 0.03642870342901361 1101 3 -0.9730163245080199 1101 4 -0.0807598927805202 1101 5 -0.24259458572745984 1101 6 -1.926999476983518 1101 7 -0.522008538415097 1101 8 0.05837661528334337 1101 9 -0.09559432194185291 1101 10 -9.039751742287677 1101 11 -3.8527782977389045 1101 12 -1.367619073999883 1101 13 -0.5252790974023327 1101 14 -0.29459392970693443 1101 15 -1.552851388101054 1101 16 -0.25590132703442237 1101 17 -0.008141262355039203 1101 18 -0.08612357465926834 1101 19 -0.7465037420206855 1101 20 -0.17029524617503 1101 21 -0.1371511799079741 1101 22 0.1597645270299708 1101 23 0.019289928167751214 1101 24 -0.005488931128219576 1101 25 0.07373332038427094 1102 1 -0.3384861382291824 1102 2 -0.45131319607463993 1102 3 -0.6082792769367735 1102 4 -0.3468725840228757 1102 5 -0.446435234138967 1102 6 -0.9443400231512342 1102 7 -0.2756400518090345 1102 8 0.1529316689148601 1102 9 0.07485164686449508 1102 10 0.061161015877885916 1102 11 -0.13381486383486418 1102 12 -0.01817955584696307 1102 13 -0.24564605964648933 1102 14 -0.45705744726497954 1102 15 -0.002311355011251237 1102 16 -0.043204507745663534 1102 17 -0.10785869770908363 1102 18 -0.3248795466258798 1102 19 0.08849523554145122 1102 20 -0.11887963447833982 1102 21 -0.12800686745095646 1102 22 -0.370295146161709 1102 23 -0.490703937205069 1102 24 -3.455121782986268 1102 25 -1.7752414532851688 1103 1 0.26670571778718966 1103 2 0.1069271475174709 1103 3 0.3856884942144774 1103 4 0.31541753637486636 1103 5 0.09579131716156297 1103 6 0.7355721041540448 1103 7 0.46574118999087233 1103 8 0.7862891159738368 1103 9 0.6164497223233667 1103 10 0.749771172882923 1103 11 0.5290847452189809 1103 12 0.34876679206283256 1103 13 0.43369455337378326 1103 14 0.5619639152174827 1103 15 0.9534845783133505 1103 16 0.8590114866796981 1103 17 0.5276575923467561 1103 18 0.45391214686019055 1103 19 -0.9954072386787018 1103 20 0.18099827791858303 1103 21 0.2757214563033372 1103 22 0.4686175716450407 1103 23 0.5091479627476545 1103 24 1.9594653121832053 1103 25 1.1418491022988704 1104 1 -0.11835364623672007 1104 2 -0.07058106605441239 1104 3 -1.279641799621956 1104 4 -0.33222651621407095 1104 5 -0.1429698731976643 1104 6 -1.4810582860312493 1104 7 0.08161403647726928 1104 8 0.4501959488928904 1104 9 0.49142373388890803 1104 10 0.38988455911920283 1104 11 0.42942346881427085 1104 12 0.32329536646150814 1104 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-3.139801809815271 2216 12 -2.8041012317293976 2216 13 -2.4736966770038435 2216 14 -2.679576952322087 2216 15 -3.615533174250075 2216 16 -2.5105909291870248 2216 17 -3.0345074613428054 2216 18 -2.7317094546003275 2216 19 -2.204361388250426 2216 20 -3.2295943684762705 2216 21 -5.696206852917133 2216 22 -4.26068201022418 2216 23 -4.274886508503403 2216 24 -4.587736352487688 2216 25 -3.7755651612609356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35127.0 3 2.0 4 281.0 5 1746.0 6 2205.0 7 1842.0 8 1387.0 9 1353.0 10 1267.0 11 1217.0 12 1237.0 13 1264.0 14 1269.0 15 1338.0 16 1341.0 17 1526.0 18 1713.0 19 2187.0 20 2867.0 21 3600.0 22 4584.0 23 5684.0 24 6626.0 25 7777.0 26 8832.0 27 9608.0 28 10837.0 29 12994.0 30 15796.0 31 19062.0 32 25037.0 33 33579.0 34 46039.0 35 65040.0 36 97650.0 37 163668.0 38 264348.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19130082889118 18.646911891937147 12.173932256053883 25.987855023117785 2 14.039243752054958 19.947764844206556 43.095281524950536 22.91770987878795 3 19.039397706544598 24.288901023573562 29.22877624286926 27.44292502701258 4 11.24507876872564 16.831626842087545 39.4181482122187 32.50514617696811 5 12.085217587064417 38.73352619736419 36.88855313682554 12.29270307874585 6 30.94095626230779 38.18840941292787 18.896061623369164 11.974572701395182 7 27.331761485698593 31.250712801093 23.278512625598463 18.139013087609946 8 23.702633134579848 39.05145248302217 20.93195369555807 16.313960686839913 9 25.977133401641485 15.431878864816003 20.58179514284952 38.009192590693 10 13.609632960974901 30.017466995502684 35.9466125054403 20.426287538082114 11 34.25136457308356 22.38352394036723 25.362609769229337 18.002501717319866 12 22.958074813442146 24.898787069344635 33.09619318702664 19.046944930186573 13 29.10034798813197 22.617952196015136 25.810299474014602 22.47140034183829 14 19.451680616200797 23.23667870739861 28.21905854859091 29.09258212780968 15 22.00464599254135 33.251800264092886 22.967378263159972 21.776175480205797 16 20.699230426468134 28.720920517628713 29.89593583483411 20.683913221069048 17 20.526489236281943 29.730071572458993 29.03500181019838 20.708437381060683 18 20.37527819227815 28.28266888214858 31.42789511425899 19.914157811314272 19 23.164295495160953 27.378211525518136 27.624805625826816 21.832687353494094 20 22.61568809468554 28.47006265954978 29.05407287073567 19.86017637502901 21 21.730226103423227 27.605364473705585 27.594457298408713 23.06995212446247 22 21.849218265912587 29.73037890373891 27.890977277843405 20.529425552505103 23 20.837713710173382 29.232488355739978 28.6185393792977 21.311258554788942 24 22.59888120099743 27.674900820011672 29.21791981360312 20.508298165387778 25 21.500620201272426 28.420882855641043 29.092023016487257 20.986473926599267 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 199.0 1 199.0 2 289.5 3 380.0 4 380.0 5 380.0 6 565.0 7 750.0 8 750.0 9 750.0 10 1163.0 11 1576.0 12 1576.0 13 1576.0 14 2854.0 15 4132.0 16 4132.0 17 4132.0 18 7407.0 19 10682.0 20 10682.0 21 10682.0 22 16961.0 23 23240.0 24 23240.0 25 23240.0 26 34184.0 27 45128.0 28 45128.0 29 45128.0 30 59978.5 31 74829.0 32 74829.0 33 74829.0 34 84486.5 35 94144.0 36 94144.0 37 94144.0 38 100553.5 39 106963.0 40 106963.0 41 106963.0 42 110319.5 43 113676.0 44 113676.0 45 113676.0 46 114377.5 47 115079.0 48 115079.0 49 115079.0 50 107373.0 51 99667.0 52 99667.0 53 99667.0 54 85507.0 55 71347.0 56 71347.0 57 71347.0 58 59669.5 59 47992.0 60 47992.0 61 47992.0 62 37967.5 63 27943.0 64 27943.0 65 27943.0 66 21200.0 67 14457.0 68 14457.0 69 14457.0 70 10295.5 71 6134.0 72 6134.0 73 6134.0 74 4280.5 75 2427.0 76 2427.0 77 2427.0 78 1630.5 79 834.0 80 834.0 81 834.0 82 550.0 83 266.0 84 266.0 85 266.0 86 166.0 87 66.0 88 66.0 89 66.0 90 38.5 91 11.0 92 11.0 93 11.0 94 7.5 95 4.0 96 4.0 97 4.0 98 4.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23041314259858692 2 0.14003457357326002 3 0.14154281670205238 4 0.07088742705324098 5 0.021231422505307858 6 0.020767347696448668 7 0.3067534486559233 8 0.2986321395008876 9 0.017286786630004755 10 0.035385704175513094 11 0.014154281670205238 12 0.015430487394568003 13 0.013806225563560846 14 0.015430487394568003 15 0.012994094648057267 16 0.01786688014107874 17 0.016938730523360365 18 0.013458169456916455 19 0.022043553420811435 20 0.015082431287923614 21 0.012994094648057267 22 0.014850393883494017 23 0.015314468692353207 24 0.013110113350272063 25 0.013110113350272063 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 861930.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.89095580764835 #Duplication Level Percentage of deduplicated Percentage of total 1 93.10355108707978 78.1054588978134 2 5.2379897697021285 8.788399365819908 3 0.8503651977832193 2.1401384768278264 4 0.27428438926627385 0.9203991831465919 5 0.13668747997138278 0.5733421670869046 6 0.08564358247070336 0.43108331953550777 7 0.052315223121088564 0.30721418496429526 8 0.03758315968970008 0.2522309750912336 9 0.03098617500643586 0.23395138543016716 >10 0.15655296887903725 2.516731848272522 >50 0.017714329508733133 1.0357958289867097 >100 0.014112856333592985 2.5836609479089394 >500 0.0013836132424166422 0.7874367719482794 >1k 8.301679454499855E-4 1.3241566471677437 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2609 0.30269279407840544 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2069 0.24004269488241506 No Hit CTGTAGGACGTGGAATATGGCAAGA 2008 0.2329655540473124 No Hit GTCCTACAGTGTGCATTTCTCATTT 1908 0.2213636838258327 No Hit CTTTAGGACGTGAAATATGGCGAGG 1474 0.17101156706461082 No Hit CTGTAGGACCTGGAATATGGCGAGA 1340 0.15546506096782803 No Hit GTATCAACGCAGAGTACTTTTTTTT 953 0.11056582321070157 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1601870221479702E-4 0.0 10 0.0 0.0 0.0 1.1601870221479702E-4 0.0 11 0.0 0.0 0.0 1.1601870221479702E-4 0.0 12 0.0 0.0 0.0 3.480561066443911E-4 0.0 13 0.0 0.0 0.0 3.480561066443911E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACCGT 25 0.005965948 19.035122 6 TAGGACC 610 0.0 14.0504875 4 TCCAACG 115 2.5465852E-11 14.017186 18 TTACACC 50 0.0014714065 13.332352 4 CTAGTAC 60 3.9626312E-4 12.713777 3 TGTAGGA 1335 0.0 12.21451 2 CCGGTTT 55 0.0031112265 12.067572 12 GGCGAGG 475 0.0 11.977534 19 GTAGGAC 1295 0.0 11.92837 3 AGGACCT 985 0.0 11.788248 5 CCAACGA 90 7.606668E-6 11.589374 19 CTGTAGG 1395 0.0 11.489446 1 TCTAGTA 75 2.0004406E-4 11.443068 2 TAGTACC 75 2.0255278E-4 11.427729 4 GGACCTG 925 0.0 11.421073 6 GTCCTAA 675 0.0 11.307074 1 GTCCTAC 1400 0.0 11.243976 1 GACAGTA 60 0.0057918625 11.103821 7 TGGCGAG 1190 0.0 11.075864 18 TGCCGGT 60 0.005951958 11.063225 10 >>END_MODULE