FastQCFastQC Report
Thu 26 May 2016
SRR1926741_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926741_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861930
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT26240.3044330746116274No Hit
CTGTAGGACGTGGAATATGGCAAGA23310.27043959486269187No Hit
GTCCTACAGTGTGCATTTCTCATTT19410.225192300998921No Hit
GTCCTAAAGTGTGTATTTCTCATTT19160.22229183344355108No Hit
CTTTAGGACGTGAAATATGGCGAGG18480.2144025616929449No Hit
CTGTAGGACCTGGAATATGGCGAGA15860.18400566171266808No Hit
GTATCAACGCAGAGTACTTTTTTTT14830.172055735384544No Hit
CTGAAGGACCTGGAATATGGCGAGA10260.11903518847238174No Hit
GTACATGGGAAGCAGTGGTATCAAC9080.1053449816110357No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCCG551.1391192E-515.52812911
TAGGACC5400.014.4534524
CCAACGA1051.3897079E-913.55630319
TCCAACG1550.013.46884318
GGACCTA1009.782525E-913.3253776
GCCGGTT655.5042747E-513.13918711
GATATAC2650.012.5901581
GGCGAGG5800.012.43440219
AAAGGCG550.003023058412.1139785
AAGGCGT550.003023058412.1139786
TAGAGTG1501.2732926E-1112.05629355
GACCTAG951.0520507E-611.9866267
AGGACCT8900.011.9778665
TGTAGGA13400.011.9505592
GTAGGAC12850.011.8534323
GTCCTAG1950.011.7323681
CTGTAGG12900.011.5277341
TTCCCGT752.0933384E-411.38729612
TGGCGAG13700.011.35958818
GTCCTAC13600.011.3549471
Produced by FastQC (version 0.11.5)