FastQCFastQC Report
Thu 26 May 2016
SRR1926740_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926740_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783395
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT25420.32448509372666406No Hit
GTCCTAAAGTGTGTATTTCTCATTT19470.24853362607624505No Hit
CTGTAGGACGTGGAATATGGCAAGA18800.2399811078702315No Hit
GTCCTACAGTGTGCATTTCTCATTT16660.21266410942117325No Hit
CTTTAGGACGTGAAATATGGCGAGG13560.1730927565276776No Hit
CTGTAGGACCTGGAATATGGCGAGA10230.13058546454853553No Hit
GTACATGGGAAGCAGTGGTATCAAC8530.10888504521984439No Hit
CTGAAGGACCTGGAATATGGCGAGA8020.10237491942123705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTC456.6948636E-414.7957975
AATCCCG1152.5465852E-1114.02290919
CACGTTT802.028908E-613.04336718
ACGTTTT854.001293E-612.27611119
GGCGAGG4150.012.11475919
GTCCTAT959.919931E-712.0446961
GTCCTAC11900.012.0193921
TAGGACC4500.011.8449764
TGTAGGA13300.011.8220262
GATATAC2200.011.70251751
CTGTAGG12200.011.6456751
TCCTACA12800.011.5393512
GTAGGAC12150.011.4500763
GACGTGG5500.011.4139017
AGGACCT7000.011.2780215
TAATACT1104.833819E-711.2488824
CTATACT1104.833819E-711.2488824
CTTTTAG855.1172203E-511.21811
CCTACAG12450.011.17417053
AGGACGT10250.011.1355125
Produced by FastQC (version 0.11.5)