##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926740_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 783395 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.741097402970404 31.0 30.0 33.0 23.0 34.0 2 28.787460987113782 31.0 30.0 31.0 23.0 34.0 3 28.480867250875995 31.0 28.0 33.0 16.0 34.0 4 32.366569865776526 35.0 33.0 37.0 24.0 37.0 5 32.271666273080626 35.0 33.0 37.0 22.0 37.0 6 31.827553150071164 35.0 32.0 37.0 17.0 37.0 7 31.875176635030858 35.0 32.0 37.0 18.0 37.0 8 31.729352370132563 35.0 32.0 37.0 17.0 37.0 9 33.88302325136106 38.0 34.0 39.0 24.0 39.0 10 34.01445758525393 38.0 34.0 39.0 25.0 39.0 11 34.18567134076679 38.0 35.0 39.0 26.0 39.0 12 34.0599518761289 38.0 35.0 39.0 25.0 39.0 13 34.15634130930118 38.0 35.0 39.0 25.0 39.0 14 35.21228754332106 39.0 36.0 41.0 25.0 41.0 15 34.815534947248835 39.0 35.0 41.0 21.0 41.0 16 34.89680174113953 39.0 35.0 41.0 23.0 41.0 17 35.126777679203975 39.0 36.0 41.0 25.0 41.0 18 35.18600195303774 40.0 36.0 41.0 25.0 41.0 19 34.53776702685108 39.0 34.0 41.0 18.0 41.0 20 34.847702627665484 39.0 35.0 41.0 22.0 41.0 21 34.854969715150084 39.0 35.0 41.0 22.0 41.0 22 34.87827979499486 39.0 35.0 41.0 22.0 41.0 23 34.89046649519081 40.0 36.0 41.0 22.0 41.0 24 33.58067003235915 39.0 33.0 41.0 8.0 41.0 25 34.19287205049815 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.024533165238157295 1101 2 -0.18319606033685787 1101 3 -1.2800263581362046 1101 4 -0.4034845787137442 1101 5 -0.16815252353343624 1101 6 -2.297332546922412 1101 7 -0.6092055213463858 1101 8 0.008419060354817987 1101 9 -0.055070019646692 1101 10 -9.394023630991754 1101 11 -4.065740645763459 1101 12 -1.52468543073239 1101 13 -0.9146492645280659 1101 14 -0.5387081055655543 1101 15 -1.9598611262190389 1101 16 -0.4955682092434799 1101 17 -0.37476821808292016 1101 18 -0.171860859417869 1101 19 -0.8757482535315333 1101 20 -0.2606336390059809 1101 21 -0.47400107359914756 1101 22 -0.01256233438972032 1101 23 -0.12866102629910614 1101 24 -0.46190227612136425 1101 25 -0.31541977582472924 1102 1 -0.08878158890664167 1102 2 -0.1925342585307277 1102 3 -0.37537716698219725 1102 4 -0.18634315337367724 1102 5 0.035267523765085684 1102 6 -0.7614793714338965 1102 7 0.011504917623756938 1102 8 0.39715391058187066 1102 9 0.26897637272513464 1102 10 0.3919961877086706 1102 11 0.12109127971218925 1102 12 0.19155842097402598 1102 13 0.04167202221152877 1102 14 -0.1560321347959075 1102 15 0.3876491795738275 1102 16 0.3012932005821227 1102 17 0.18041488350593937 1102 18 -0.06832582658692843 1102 19 0.4172266452983635 1102 20 0.2791963197022014 1102 21 0.18380335488843258 1102 22 -0.06271936687056012 1102 23 -0.29337042181845163 1102 24 -3.4177382209420344 1102 25 -1.677809552752791 1103 1 0.2483055723660712 1103 2 0.1467904117683787 1103 3 0.4147316334489979 1103 4 0.2913912823601308 1103 5 -0.054448434207330365 1103 6 0.82662686532883 1103 7 0.7391977196581045 1103 8 0.7602875288440387 1103 9 0.528259305672421 1103 10 0.5810090371466998 1103 11 0.3006959495966868 1103 12 0.12516631790211363 1103 13 0.2435681694531695 1103 14 0.40439615677127705 1103 15 0.6606384100932345 1103 16 0.5859757997687964 1103 17 0.15191601158916512 1103 18 0.0708378370404219 1103 19 -1.5203656484939003 1103 20 -0.13041156340557336 1103 21 0.14947161895319283 1103 22 0.16719608735137825 1103 23 0.23637400600323133 1103 24 1.6825447500111181 1103 25 0.9022912664463902 1104 1 -0.3911853291875005 1104 2 -0.23520191899182663 1104 3 -1.2571145003381154 1104 4 -0.3265528665849331 1104 5 -0.4583479116693283 1104 6 -1.2052108436612947 1104 7 -0.19611454945398066 1104 8 0.0181150679006592 1104 9 -0.12051425116320047 1104 10 0.07923214048084759 1104 11 -0.05643654370861384 1104 12 -0.12868618797351417 1104 13 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0.2955566000086307 2215 2 -0.07244760439104425 2215 3 0.1263575669955408 2215 4 0.38422812328882117 2215 5 0.6115050036619252 2215 6 1.059661068375494 2215 7 0.6098174075102527 2215 8 -0.6956664108706399 2215 9 -0.0830659768437414 2215 10 -0.2105882455884469 2215 11 -0.2784701658003925 2215 12 -0.35879972084947553 2215 13 -0.11402406745436622 2215 14 -0.1674338909952766 2215 15 -0.34940306058759063 2215 16 0.025742943492765846 2215 17 -0.6327694910090216 2215 18 -1.1461692481044992 2215 19 0.07436223723134106 2215 20 -3.8566822588264635 2215 21 -2.076331447778557 2215 22 -1.1918241278946837 2215 23 -1.2220663496683102 2215 24 -1.064547968211805 2215 25 -0.778630851706275 2216 1 -1.0974436349636854 2216 2 -1.69787537450377 2216 3 -0.96810130381186 2216 4 -1.0435623235803284 2216 5 -1.0098800376389825 2216 6 -2.4156962100426043 2216 7 -10.638822464279212 2216 8 -11.927807761780706 2216 9 -6.63898938791877 2216 10 -4.041120488007579 2216 11 -2.990232940160695 2216 12 -2.7862610558537213 2216 13 -2.4831339441240274 2216 14 -2.824016580163054 2216 15 -3.757253244649238 2216 16 -2.5301322681329452 2216 17 -3.203692721336118 2216 18 -2.7090401579293513 2216 19 -2.2882023048437716 2216 20 -3.371855294849528 2216 21 -6.123172275122105 2216 22 -4.479218616287657 2216 23 -4.384884842592026 2216 24 -4.843792230971101 2216 25 -4.301008952286473 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36419.0 3 6.0 4 304.0 5 1719.0 6 2166.0 7 1812.0 8 1487.0 9 1274.0 10 1333.0 11 1264.0 12 1201.0 13 1224.0 14 1201.0 15 1313.0 16 1304.0 17 1432.0 18 1732.0 19 2201.0 20 2767.0 21 3532.0 22 4407.0 23 5542.0 24 6353.0 25 7394.0 26 8268.0 27 9296.0 28 10434.0 29 12377.0 30 14825.0 31 18531.0 32 24065.0 33 31505.0 34 43832.0 35 61486.0 36 91175.0 37 150015.0 38 218199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83696725631565 18.971917902848634 12.268775553019799 25.92233928781591 2 13.96047374246836 20.00457586347055 43.184519777188676 22.850430616872412 3 18.840696415538332 24.357415935854714 29.403629326553748 27.398258322053202 4 11.294461468111386 17.096118646514164 39.246542009996205 32.362877875378246 5 12.10635173954676 38.81634216406001 36.746632620491546 12.330673475901692 6 30.78047578496348 38.148082420367366 19.03433501052661 12.037106784142544 7 27.393222316612594 31.20784418083375 23.454116045566668 17.944817456986993 8 23.638754943265198 38.86893742347615 21.12512618040247 16.367181452856187 9 25.930871945980382 15.44842078922876 20.668963870742054 37.951743394048805 10 13.568262700014941 29.869153102490138 36.078730324122844 20.483853873372077 11 33.99277911970626 22.455712104421895 25.52152220650492 18.029986569366923 12 23.039427841165153 24.824232489317342 33.081826526690996 19.0545131428265 13 28.888205164868275 22.594256037339843 25.863151928016627 22.654386869775255 14 19.336586312464732 23.303356900835205 28.213593571663846 29.146463215036217 15 22.063121019189598 33.22349732984888 23.045006376956653 21.66837527400487 16 20.54630995014395 28.79585830923518 29.93546163126951 20.72237010935136 17 20.58848312454996 29.711924909481812 29.08468110531772 20.614910860650507 18 20.39372390813109 28.197602420559438 31.468932323915794 19.939741347393685 19 23.127757803562673 27.30442576401373 27.804022307566594 21.763794124857004 20 22.731282707306395 28.363035621041448 29.07415422209115 19.83152744956101 21 21.826326970411905 27.606448713627685 27.53304020998662 23.034184105973793 22 21.950101624978295 29.70347465503682 27.88839636805605 20.45802735192883 23 20.71824438795768 29.28667089679051 28.734243958987378 21.260840756264436 24 22.64037728429863 27.580232917391186 29.37320147788408 20.406188320426104 25 21.510907185196817 28.381533810550614 29.027526168435486 21.080032835817082 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 164.0 1 164.0 2 228.5 3 293.0 4 293.0 5 293.0 6 501.0 7 709.0 8 709.0 9 709.0 10 1055.0 11 1401.0 12 1401.0 13 1401.0 14 2588.0 15 3775.0 16 3775.0 17 3775.0 18 6813.0 19 9851.0 20 9851.0 21 9851.0 22 15839.5 23 21828.0 24 21828.0 25 21828.0 26 31485.0 27 41142.0 28 41142.0 29 41142.0 30 54241.0 31 67340.0 32 67340.0 33 67340.0 34 76585.5 35 85831.0 36 85831.0 37 85831.0 38 91366.0 39 96901.0 40 96901.0 41 96901.0 42 100593.5 43 104286.0 44 104286.0 45 104286.0 46 104900.5 47 105515.0 48 105515.0 49 105515.0 50 98071.0 51 90627.0 52 90627.0 53 90627.0 54 77586.5 55 64546.0 56 64546.0 57 64546.0 58 53707.5 59 42869.0 60 42869.0 61 42869.0 62 33920.5 63 24972.0 64 24972.0 65 24972.0 66 18826.5 67 12681.0 68 12681.0 69 12681.0 70 9121.0 71 5561.0 72 5561.0 73 5561.0 74 3819.0 75 2077.0 76 2077.0 77 2077.0 78 1412.5 79 748.0 80 748.0 81 748.0 82 470.0 83 192.0 84 192.0 85 192.0 86 131.0 87 70.0 88 70.0 89 70.0 90 40.0 91 10.0 92 10.0 93 10.0 94 6.5 95 3.0 96 3.0 97 3.0 98 3.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2153447494558939 2 0.1313513617013129 3 0.133521403634182 4 0.06356946368051877 5 0.018509181192118918 6 0.01812623261573025 7 0.22606730959477658 8 0.22670555722209104 9 0.01582854115739825 10 0.0339547737731285 11 0.01416909732638069 12 0.014807344953695133 13 0.013147901122677576 14 0.014424396377306466 15 0.013530849699066243 16 0.017870933564804473 17 0.016211489733786916 18 0.013403200173603354 19 0.020806872650450923 20 0.01557324210647247 21 0.013658499224529134 22 0.015700891631935356 23 0.01582854115739825 24 0.014424396377306466 25 0.013275550648140467 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 783395.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.34139123120995 #Duplication Level Percentage of deduplicated Percentage of total 1 93.42027134463244 78.79195654403436 2 5.03495603256519 8.493103931490428 3 0.7663834345250176 1.9391353525317865 4 0.25833445005698763 0.8715314768302346 5 0.13538911124178363 0.5709452999844539 6 0.07840790775300466 0.3967819214050072 7 0.04439902872019149 0.2621273096102778 8 0.038500565312762546 0.25977529933331656 9 0.028368881629077303 0.2153403849842904 >10 0.1616452219014495 2.5790578643457844 >50 0.016824496967187838 1.000947275069429 >100 0.014549306188928744 2.6589911916271602 >500 0.001060886887902499 0.6291488565574069 >1k 9.093316182021419E-4 1.331157292196025 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2542 0.32448509372666406 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1947 0.24853362607624505 No Hit CTGTAGGACGTGGAATATGGCAAGA 1880 0.2399811078702315 No Hit GTCCTACAGTGTGCATTTCTCATTT 1666 0.21266410942117325 No Hit CTTTAGGACGTGAAATATGGCGAGG 1356 0.1730927565276776 No Hit CTGTAGGACCTGGAATATGGCGAGA 1023 0.13058546454853553 No Hit GTACATGGGAAGCAGTGGTATCAAC 853 0.10888504521984439 No Hit CTGAAGGACCTGGAATATGGCGAGA 802 0.10237491942123705 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.276495254628891E-4 13 0.0 0.0 0.0 0.0 1.276495254628891E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACTC 45 6.6948636E-4 14.795797 5 AATCCCG 115 2.5465852E-11 14.022909 19 CACGTTT 80 2.028908E-6 13.043367 18 ACGTTTT 85 4.001293E-6 12.276111 19 GGCGAGG 415 0.0 12.114759 19 GTCCTAT 95 9.919931E-7 12.044696 1 GTCCTAC 1190 0.0 12.019392 1 TAGGACC 450 0.0 11.844976 4 TGTAGGA 1330 0.0 11.822026 2 GATATAC 220 0.0 11.7025175 1 CTGTAGG 1220 0.0 11.645675 1 TCCTACA 1280 0.0 11.539351 2 GTAGGAC 1215 0.0 11.450076 3 GACGTGG 550 0.0 11.413901 7 AGGACCT 700 0.0 11.278021 5 TAATACT 110 4.833819E-7 11.248882 4 CTATACT 110 4.833819E-7 11.248882 4 CTTTTAG 85 5.1172203E-5 11.2181 1 CCTACAG 1245 0.0 11.1741705 3 AGGACGT 1025 0.0 11.135512 5 >>END_MODULE