FastQCFastQC Report
Thu 26 May 2016
SRR1926740_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926740_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783395
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT24520.3129966364350041No Hit
CTGTAGGACGTGGAATATGGCAAGA22780.29078561900446137No Hit
GTCCTAAAGTGTGTATTTCTCATTT19630.2505760184836513No Hit
CTTTAGGACGTGAAATATGGCGAGG18120.23130094013875505No Hit
GTCCTACAGTGTGCATTTCTCATTT17170.21917423521978058No Hit
CTGTAGGACCTGGAATATGGCGAGA13210.16862502313647648No Hit
GTATCAACGCAGAGTACTTTTTTTT12910.1647955373725898No Hit
CTGAAGGACCTGGAATATGGCGAGA10070.1285430721411293No Hit
GTACATGGGAAGCAGTGGTATCAAC9430.12037350251150442No Hit
CCCATGTACTCTGCGTTGATACCAC8170.10428966230318039No Hit
GGTATCAACGCAGAGTACTTTTTTT7930.10122607369207105No Hit
TATCAACGCAGAGTACTTTTTTTTT7860.10033252701383082No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGA456.8212237E-414.75935910
TAGGACC5050.014.1418754
CCCCTAT551.9093929E-413.85934451
TCTAGGC655.3277672E-513.1845793
GGCGAGG3800.012.48601419
TTAGCTG701.06607986E-412.2428235
TAGGACT1103.680907E-812.1191594
ATTTAGA2600.012.0936111
TAATACT1156.8277586E-811.5922384
AGGACCT8550.011.5825555
TAGTACA752.0268813E-411.4266364
TGTAGGA13500.011.3626512
GGACCTG8400.011.3352216
GTCCTAC12400.011.2188041
GTCCAAT855.156133E-511.20976451
TATGAAC855.189912E-511.2025833
CTAGCAC855.189912E-511.2025833
TTTAGAA3350.011.0919362
CCTACAG13500.011.0739613
CTGTAGG13600.011.0696421