##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926740_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 783395 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.783697879103133 31.0 30.0 33.0 25.0 34.0 2 30.156041332916345 31.0 30.0 33.0 26.0 34.0 3 30.225792863115032 31.0 30.0 34.0 26.0 34.0 4 33.81252752442893 35.0 35.0 37.0 28.0 37.0 5 33.77170137670013 37.0 35.0 37.0 28.0 37.0 6 33.7600010211962 37.0 35.0 37.0 29.0 37.0 7 33.757650993432435 37.0 35.0 37.0 29.0 37.0 8 33.78020538808647 37.0 35.0 37.0 29.0 37.0 9 35.31380593442644 39.0 35.0 39.0 30.0 39.0 10 35.19095986060672 39.0 35.0 39.0 29.0 39.0 11 35.21505498503309 39.0 35.0 39.0 29.0 39.0 12 35.11111252943917 39.0 35.0 39.0 29.0 39.0 13 35.127721009197145 39.0 35.0 39.0 29.0 39.0 14 36.213954646123604 40.0 36.0 41.0 29.0 41.0 15 36.21649742467083 40.0 36.0 41.0 29.0 41.0 16 36.20009318415359 40.0 36.0 41.0 29.0 41.0 17 36.18246478468716 40.0 36.0 41.0 29.0 41.0 18 36.15160040592549 40.0 36.0 41.0 29.0 41.0 19 36.21992736742001 40.0 37.0 41.0 29.0 41.0 20 36.187352485017136 40.0 37.0 41.0 29.0 41.0 21 36.1278180228365 40.0 37.0 41.0 29.0 41.0 22 36.04823492618666 40.0 36.0 41.0 28.0 41.0 23 35.97363782000141 40.0 36.0 41.0 28.0 41.0 24 35.9046943112989 40.0 36.0 41.0 28.0 41.0 25 35.78017985818138 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12838010185495463 1101 2 0.14295617840938135 1101 3 0.22663443149080464 1101 4 0.34382051101565025 1101 5 0.08828054384583339 1101 6 0.16173345065723765 1101 7 0.13055712099577477 1101 8 0.15258684357949193 1101 9 0.3889418534317244 1101 10 0.3392382340422486 1101 11 0.17205443411144472 1101 12 0.23983340255077223 1101 13 0.18167346674793095 1101 14 0.12366831527936029 1101 15 0.14919001194274273 1101 16 0.1752467650952525 1101 17 0.16993637310183374 1101 18 0.2997815672604034 1101 19 0.09994940114751216 1101 20 0.24386005401355249 1101 21 0.37958468868867357 1101 22 0.19945604016397311 1101 23 0.28913987217890735 1101 24 0.2176745119634802 1101 25 0.13873086960536085 1102 1 -0.29842491357370093 1102 2 -0.2871123666975528 1102 3 0.07259500689900378 1102 4 0.009316956761360018 1102 5 -0.3842893999657164 1102 6 -0.1803021708309842 1102 7 -0.13321282556169223 1102 8 -0.06735542338601874 1102 9 0.0036510773308648936 1102 10 0.026934782073595898 1102 11 0.07608167366921492 1102 12 0.15114488977708618 1102 13 0.1398269707175075 1102 14 0.023557448631684963 1102 15 0.014212785942973483 1102 16 -0.054175801587774686 1102 17 0.16061053156219174 1102 18 0.14528425998130245 1102 19 0.05273599829655495 1102 20 0.06173120843397584 1102 21 -2.574893775744158E-4 1102 22 -0.0306159373469157 1102 23 0.058003849442663125 1102 24 0.2355216395562323 1102 25 0.096458069004008 1103 1 -0.34479137996096654 1103 2 -0.279540907030416 1103 3 -0.18021310839006688 1103 4 -0.22588269496669966 1103 5 -0.3305802001274145 1103 6 -0.2582314730087205 1103 7 -0.3489928614254083 1103 8 -0.31695100641329077 1103 9 -0.310871655360053 1103 10 -0.1956372476815389 1103 11 -0.09705854379344458 1103 12 -0.3018629290662247 1103 13 -0.23726585688442725 1103 14 -0.2142431864117924 1103 15 -0.189455125703752 1103 16 -0.03206398832787016 1103 17 -0.09049422988189093 1103 18 -0.10941862051788576 1103 19 -0.14817061437874912 1103 20 -0.18186082968245643 1103 21 -0.04978811692353702 1103 22 -0.03091445615464039 1103 23 -0.019477674523486144 1103 24 -0.18438237835489701 1103 25 -0.04252869458913722 1104 1 -0.07241234433276844 1104 2 -0.0025596588900569373 1104 3 0.21399049755677169 1104 4 0.25298744614865143 1104 5 0.19879124984032615 1104 6 0.15376182727177223 1104 7 0.0523952241732033 1104 8 0.20782625465846394 1104 9 0.0617744998233718 1104 10 0.2902248062973385 1104 11 0.35569138349253393 1104 12 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0.23924275058234912 2212 23 0.010051821864443866 2212 24 0.033438799630971516 2212 25 0.1303565190447955 2213 1 0.10483211495184364 2213 2 0.2187934860659091 2213 3 0.39605151846749465 2213 4 0.33849184950438627 2213 5 0.39003462393774413 2213 6 0.38618388161460615 2213 7 0.36359352126406463 2213 8 0.017142169346051617 2213 9 0.1446828015054109 2213 10 0.25724346316949465 2213 11 0.27332567540788943 2213 12 0.25111004992427155 2213 13 0.16423610340425654 2213 14 0.09064440558621811 2213 15 0.14662944403792721 2213 16 0.23990175524266277 2213 17 0.10510898699538984 2213 18 0.053027002508152066 2213 19 -0.03031623825609131 2213 20 0.0994325744880129 2213 21 0.04084159184573366 2213 22 -0.0032497306769059264 2213 23 0.04577207483328749 2213 24 -0.10494468357842379 2213 25 0.013951325003922932 2214 1 0.2805549784981345 2214 2 0.24704293321758541 2214 3 0.42242118816196594 2214 4 0.30083047399415364 2214 5 0.451626367572608 2214 6 0.3670482811632638 2214 7 0.34224776951764824 2214 8 0.2970073122358414 2214 9 0.15944902164989116 2214 10 0.08714192289462375 2214 11 0.32436036031383964 2214 12 0.12253376865442078 2214 13 0.19395422372615911 2214 14 0.44142752199269353 2214 15 0.29090469264113494 2214 16 0.3997076792430221 2214 17 0.27566510179905634 2214 18 0.4211013391056895 2214 19 0.34245661047926745 2214 20 0.38714347502375546 2214 21 0.43110065585690904 2214 22 0.5215745719206879 2214 23 0.38278506063652884 2214 24 0.3828734021535851 2214 25 0.5065176031501295 2215 1 0.45281892338178054 2215 2 0.38199629112096645 2215 3 0.22438835791101397 2215 4 0.43179485566265896 2215 5 0.4848410534824552 2215 6 0.44071145804227996 2215 7 0.4300126634364716 2215 8 0.4419807015491699 2215 9 0.12125831347990612 2215 10 0.2156433249697045 2215 11 -0.011548646033048726 2215 12 0.2390169428263249 2215 13 0.261399690514871 2215 14 0.4829934702027572 2215 15 0.4795732559360033 2215 16 0.36492311424477464 2215 17 0.36827004419870946 2215 18 0.18474491721860886 2215 19 0.2221593717976944 2215 20 0.1785782394399078 2215 21 0.08268082568815771 2215 22 -0.0626812713979632 2215 23 0.1347713594240716 2215 24 -0.05277113519253618 2215 25 -0.06487450258308058 2216 1 -1.2004395100156202 2216 2 -0.8710131619960286 2216 3 -2.568498608355398 2216 4 -2.3629673704289047 2216 5 -1.4608728104866557 2216 6 -1.2259998935305276 2216 7 -0.8796635266380619 2216 8 -0.7844798923885463 2216 9 -1.04510886390009 2216 10 -1.1271918268886836 2216 11 -0.9678769324823762 2216 12 -0.8944721851853856 2216 13 -1.1971879451213852 2216 14 -1.3700162841607977 2216 15 -1.5530969333070814 2216 16 -1.514652820975165 2216 17 -1.5676864437484213 2216 18 -1.4063949696331832 2216 19 -1.5307687447485776 2216 20 -1.4233281994040254 2216 21 -1.4590305258846499 2216 22 -1.5150421406750354 2216 23 -1.6608637285700425 2216 24 -1.796917914565583 2216 25 -1.8696674859482414 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19045.0 3 20.0 4 323.0 5 2001.0 6 2651.0 7 2019.0 8 1468.0 9 1340.0 10 1234.0 11 1069.0 12 1004.0 13 997.0 14 961.0 15 989.0 16 1009.0 17 1090.0 18 1170.0 19 1458.0 20 1861.0 21 2411.0 22 3023.0 23 3739.0 24 4374.0 25 5248.0 26 6192.0 27 6782.0 28 8120.0 29 10003.0 30 12322.0 31 16072.0 32 21184.0 33 27752.0 34 37932.0 35 52642.0 36 78335.0 37 135623.0 38 309932.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.13913859161698 19.898886369851983 10.14752979606025 28.814445242470793 2 13.601367816694163 21.039254447896074 40.976997320336686 24.382380415073072 3 18.41522017781727 24.956975574382465 29.134174926845706 27.493629320954554 4 11.715418147933034 17.66312013735089 38.33187600125096 32.28958571346511 5 12.23471139791535 39.01754654717908 36.126610880263485 12.62113117464208 6 31.408554430590513 38.78785550069483 17.753869602249075 12.049720466465581 7 28.02633242128299 31.71100729427477 22.104857274548433 18.15780300989381 8 24.203977781210543 39.82236713600519 19.700472742917473 16.273182339866796 9 26.005300664625725 15.656365776498854 19.945154972947716 38.39317858592771 10 13.950819483787127 30.42793916965274 34.91030293901009 20.71093840755005 11 34.024512763819864 22.705646412126427 25.171092759658237 18.098748064395473 12 23.373994117887342 25.103779461623297 32.31511171929251 19.207114701196843 13 29.144907574653207 22.801188170883176 25.61537116298544 22.43853309147818 14 19.645134319213167 23.64235155955808 27.692415703444624 29.02009841778413 15 21.7598212861018 33.37686293282272 22.87346417743737 21.989851603638105 16 20.732457651999642 29.120872107124164 29.315666526251295 20.8310037146249 17 20.491961271133974 30.232003012528804 28.593110755110768 20.682924961226455 18 20.4381714390752 28.420892733139986 31.23838384869899 19.90255197908582 19 22.941967898656358 27.59530963984917 27.6443271201975 21.818395341296974 20 22.880429926345755 28.50559746741725 28.646012841624223 19.967959764612772 21 21.574162019731602 27.622709724860595 27.501302163122745 23.301826092285065 22 22.16953133476726 29.865265925873917 27.378780819382303 20.586421919976512 23 20.748308003380163 29.546052178086633 28.467922909800176 21.237716908733027 24 22.58822177828554 27.534768539497957 29.318415358790904 20.558594323425602 25 21.54365217657714 28.73125230576374 28.61393759981005 21.11115791784907 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 255.0 1 255.0 2 245.5 3 236.0 4 236.0 5 236.0 6 398.5 7 561.0 8 561.0 9 561.0 10 975.5 11 1390.0 12 1390.0 13 1390.0 14 2422.0 15 3454.0 16 3454.0 17 3454.0 18 6201.0 19 8948.0 20 8948.0 21 8948.0 22 14533.0 23 20118.0 24 20118.0 25 20118.0 26 29490.0 27 38862.0 28 38862.0 29 38862.0 30 52182.0 31 65502.0 32 65502.0 33 65502.0 34 74850.5 35 84199.0 36 84199.0 37 84199.0 38 90254.0 39 96309.0 40 96309.0 41 96309.0 42 100340.0 43 104371.0 44 104371.0 45 104371.0 46 105967.5 47 107564.0 48 107564.0 49 107564.0 50 100650.5 51 93737.0 52 93737.0 53 93737.0 54 79689.5 55 65642.0 56 65642.0 57 65642.0 58 54963.5 59 44285.0 60 44285.0 61 44285.0 62 34994.0 63 25703.0 64 25703.0 65 25703.0 66 19500.0 67 13297.0 68 13297.0 69 13297.0 70 9529.5 71 5762.0 72 5762.0 73 5762.0 74 3945.5 75 2129.0 76 2129.0 77 2129.0 78 1455.5 79 782.0 80 782.0 81 782.0 82 491.5 83 201.0 84 201.0 85 201.0 86 136.0 87 71.0 88 71.0 89 71.0 90 43.5 91 16.0 92 16.0 93 16.0 94 8.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11603341864576619 2 0.10645970423604952 3 0.0151902935300838 4 0.0 5 0.006382476273144455 6 0.33571825196739835 7 0.022721615532394258 8 0.011999055393511576 9 0.012382003969900243 10 0.004595382916664007 11 0.006127177222218677 12 3.829485763886673E-4 13 7.658971527773346E-4 14 0.0 15 0.002552990509257782 16 6.382476273144455E-4 17 0.0 18 3.829485763886673E-4 19 1.276495254628891E-4 20 6.382476273144455E-4 21 0.010594910613419795 22 0.0 23 1.276495254628891E-4 24 0.0 25 0.004084784814812451 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 783395.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.96959363264745 #Duplication Level Percentage of deduplicated Percentage of total 1 91.91457332280756 75.34200224188713 2 6.250973477097734 10.247795114523168 3 0.948327682256618 2.3320210423549614 4 0.3067002435710385 1.0056037733020806 5 0.14500943094882301 0.5943182063888237 6 0.08596858098080405 0.4228085788903111 7 0.06859638300183779 0.39359723475310626 8 0.03779576688457299 0.24784829220501772 9 0.02692344099651301 0.19862131659090587 >10 0.17718538787517152 2.708281653169687 >50 0.019052199722412035 1.077935603252075 >100 0.015927244276489318 2.705815818315453 >500 0.0017176439676373908 0.9517713475017628 >1k 0.0012491956128271933 1.7715797768655055 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2452 0.3129966364350041 No Hit CTGTAGGACGTGGAATATGGCAAGA 2278 0.29078561900446137 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1963 0.2505760184836513 No Hit CTTTAGGACGTGAAATATGGCGAGG 1812 0.23130094013875505 No Hit GTCCTACAGTGTGCATTTCTCATTT 1717 0.21917423521978058 No Hit CTGTAGGACCTGGAATATGGCGAGA 1321 0.16862502313647648 No Hit GTATCAACGCAGAGTACTTTTTTTT 1291 0.1647955373725898 No Hit CTGAAGGACCTGGAATATGGCGAGA 1007 0.1285430721411293 No Hit GTACATGGGAAGCAGTGGTATCAAC 943 0.12037350251150442 No Hit CCCATGTACTCTGCGTTGATACCAC 817 0.10428966230318039 No Hit GGTATCAACGCAGAGTACTTTTTTT 793 0.10122607369207105 No Hit TATCAACGCAGAGTACTTTTTTTTT 786 0.10033252701383082 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGCGA 45 6.8212237E-4 14.759359 10 TAGGACC 505 0.0 14.141875 4 CCCCTAT 55 1.9093929E-4 13.8593445 1 TCTAGGC 65 5.3277672E-5 13.184579 3 GGCGAGG 380 0.0 12.486014 19 TTAGCTG 70 1.06607986E-4 12.242823 5 TAGGACT 110 3.680907E-8 12.119159 4 ATTTAGA 260 0.0 12.093611 1 TAATACT 115 6.8277586E-8 11.592238 4 AGGACCT 855 0.0 11.582555 5 TAGTACA 75 2.0268813E-4 11.426636 4 TGTAGGA 1350 0.0 11.362651 2 GGACCTG 840 0.0 11.335221 6 GTCCTAC 1240 0.0 11.218804 1 GTCCAAT 85 5.156133E-5 11.2097645 1 TATGAAC 85 5.189912E-5 11.202583 3 CTAGCAC 85 5.189912E-5 11.202583 3 TTTAGAA 335 0.0 11.091936 2 CCTACAG 1350 0.0 11.073961 3 CTGTAGG 1360 0.0 11.069642 1 >>END_MODULE