FastQCFastQC Report
Thu 26 May 2016
SRR1926739_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926739_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836357
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT29070.3475788449190956No Hit
CTGTAGGACGTGGAATATGGCAAGA23160.27691524074049717No Hit
GTCCTAAAGTGTGTATTTCTCATTT20150.24092582473752236No Hit
GTCCTACAGTGTGCATTTCTCATTT16900.2020668207475994No Hit
CTTTAGGACGTGAAATATGGCGAGG16410.19620807860758027No Hit
CTGTAGGACCTGGAATATGGCGAGA12370.14790334749395292No Hit
GTATCAACGCAGAGTACTTTTTTTT9300.11119653449424109No Hit
CTGAAGGACCTGGAATATGGCGAGA8600.1028269028656423No Hit
GTCCTTCAGTGTGCATTTCTCATTT8420.10067471187543119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3700.015.1818174
TAGTACG400.005200373514.2812014
TAGCGAG400.005340738714.2239918
GCCTCGA655.5407665E-513.12983816
TCGAACT655.5407665E-513.12983819
AGGACCT7100.012.6004665
GGACCTG6950.012.3246566
CTGTAGG11400.012.300451
GGCGAGG4150.012.11038519
TGTAGGA11450.011.9895932
GTAGGAC11250.011.7783273
GTATTAG657.744824E-411.7404611
ATCCCGT752.1035699E-411.38123410
GTAGTAC1104.740832E-711.2660583
CTAGAGT1451.1696102E-911.1623184
GTCCTAC14000.010.9699951
GTCTTAT1053.2930493E-610.9018571
CCACCTT2350.010.89497213
GTAAGAC700.001447877310.89464953
AGTACCT700.001467604110.8769985
Produced by FastQC (version 0.11.5)