##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926739_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 836357 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.72858121591617 31.0 30.0 33.0 23.0 34.0 2 28.789164196628953 31.0 30.0 31.0 23.0 34.0 3 28.52288317070342 31.0 28.0 33.0 16.0 34.0 4 32.36110177830759 35.0 33.0 37.0 23.0 37.0 5 32.29097622187654 35.0 33.0 37.0 22.0 37.0 6 31.843253538859603 35.0 32.0 37.0 17.0 37.0 7 31.85097990451446 35.0 32.0 37.0 17.0 37.0 8 31.705237117642348 35.0 32.0 37.0 17.0 37.0 9 33.87171626470514 38.0 34.0 39.0 23.0 39.0 10 33.9986584676161 38.0 35.0 39.0 24.0 39.0 11 34.14623539947654 38.0 35.0 39.0 25.0 39.0 12 34.01098334802004 38.0 35.0 39.0 24.0 39.0 13 34.12299412810558 39.0 35.0 39.0 25.0 39.0 14 35.20079702806338 39.0 36.0 41.0 25.0 41.0 15 34.80443160038118 39.0 35.0 41.0 19.0 41.0 16 34.8876735652359 39.0 35.0 41.0 22.0 41.0 17 35.12310532464008 39.0 36.0 41.0 25.0 41.0 18 35.173293222870136 40.0 36.0 41.0 25.0 41.0 19 34.55825323396588 39.0 34.0 41.0 18.0 41.0 20 34.838674154697095 40.0 35.0 41.0 19.0 41.0 21 34.823673383495326 39.0 35.0 41.0 19.0 41.0 22 34.86310989206762 40.0 36.0 41.0 19.0 41.0 23 34.873441604482295 40.0 36.0 41.0 19.0 41.0 24 33.62284646389042 39.0 33.0 41.0 2.0 41.0 25 34.19897125270668 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.4180639747365973 1101 2 -0.501804946565727 1101 3 -1.2816314218146765 1101 4 -0.5504685274243144 1101 5 -0.5122687855685548 1101 6 -2.1592629168099187 1101 7 -0.4943003880643069 1101 8 0.06138982849737218 1101 9 -0.18273341751618943 1101 10 -9.826524719359423 1101 11 -4.16173430761523 1101 12 -1.660237390362795 1101 13 -0.8311774253009574 1101 14 -0.7339388831146394 1101 15 -1.6876316328447842 1101 16 -0.352117280409729 1101 17 -0.25590036858839227 1101 18 -0.0367660892601549 1101 19 -0.7297356551673815 1101 20 -0.15870761948242063 1101 21 -0.4100445658042702 1101 22 0.051479096103257405 1101 23 -0.25588247402831854 1101 24 -0.2464587393275579 1101 25 -0.20588323249109663 1102 1 0.014812202175711775 1102 2 -0.16598504995464225 1102 3 -0.33246319128576474 1102 4 -0.17745124150389557 1102 5 -0.12023618406028902 1102 6 -0.8954424794282119 1102 7 -0.00599522209858705 1102 8 0.4404686980190604 1102 9 0.239408997908086 1102 10 0.40694095107568273 1102 11 0.1747060028192351 1102 12 0.11798307271890707 1102 13 -0.07585672422664658 1102 14 -0.3689173727583466 1102 15 0.32873805615856355 1102 16 0.2034984351342075 1102 17 -0.19505965766241928 1102 18 -0.2448168788655778 1102 19 0.17533567569054043 1102 20 0.12961079804068731 1102 21 0.03751540753199123 1102 22 -0.022382273866838887 1102 23 -0.20336882760128105 1102 24 -3.425877464447428 1102 25 -1.6716232717371255 1103 1 0.08533003653255022 1103 2 -0.26218937554058996 1103 3 0.1788216455213174 1103 4 0.11236616919954656 1103 5 -0.13224309018207947 1103 6 0.43331019590591424 1103 7 0.4146556319396488 1103 8 0.4302756667924328 1103 9 0.36230526094490045 1103 10 0.3520433246774317 1103 11 0.017014470567787043 1103 12 -0.08553868163042466 1103 13 0.10587226237419145 1103 14 0.24634281846688566 1103 15 0.45700239066283643 1103 16 0.4381410814213851 1103 17 0.24220699967874992 1103 18 -0.007809365273011792 1103 19 -1.4467459197838366 1103 20 -0.22383080300271985 1103 21 -0.07502167281241867 1103 22 0.010085561376577346 1103 23 0.2017905366099555 1103 24 1.5928956810099706 1103 25 0.8539282624713636 1104 1 -0.09402831546405821 1104 2 -0.027116374815125965 1104 3 -1.0234948388574914 1104 4 -0.24646705682818748 1104 5 -0.16184692843106063 1104 6 -1.2428842866111722 1104 7 -0.07739980358285337 1104 8 0.6436466031687686 1104 9 0.3043398278318392 1104 10 0.3998397415919541 1104 11 0.2570702694810407 1104 12 0.2847749272215623 1104 13 0.17956356193062106 1104 14 0.04429361061922066 1104 15 0.46646832475481403 1104 16 -0.04415217754851142 1104 17 -0.031975272382425146 1104 18 0.008784820738291899 1104 19 -0.6927627753215262 1104 20 0.10624254663053279 1104 21 0.19726333086757109 1104 22 0.28274778045643245 1104 23 0.20064638239534816 1104 24 1.3945185630409611 1104 25 0.5227890982964212 1105 1 -0.18082996573705756 1105 2 -0.21164608790892814 1105 3 0.2578414323844065 1105 4 -0.06691423582248035 1105 5 -0.16036689734785625 1105 6 -0.28783224268569185 1105 7 -0.05025311946596389 1105 8 0.28216498725089423 1105 9 -0.622099559910744 1105 10 -0.1866244922682938 1105 11 -0.41106407337493067 1105 12 -0.2576053410756032 1105 13 -0.39675261769760795 1105 14 -0.4619473149242239 1105 15 -0.07704561156550227 1105 16 -0.5932833302856722 1105 17 -0.6343172490291167 1105 18 -0.5214663499944123 1105 19 -1.1605727383079696 1105 20 -0.47049795969858366 1105 21 -0.38433648304216916 1105 22 -0.3025176026645653 1105 23 -0.582042110682508 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11 -2.8719202618418826 2216 12 -2.5093722187127376 2216 13 -2.1320725895682173 2216 14 -2.2727331799031276 2216 15 -3.3260083401168394 2216 16 -2.406987620018441 2216 17 -2.590382649873 2216 18 -2.313757137617479 2216 19 -1.845620206662403 2216 20 -2.8812574533694644 2216 21 -5.694849844504898 2216 22 -3.9963291357166035 2216 23 -3.9818399306543775 2216 24 -4.200277030209804 2216 25 -3.5713501944696873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40752.0 3 4.0 4 322.0 5 1915.0 6 2431.0 7 1874.0 8 1448.0 9 1394.0 10 1335.0 11 1371.0 12 1294.0 13 1347.0 14 1267.0 15 1376.0 16 1417.0 17 1591.0 18 1841.0 19 2244.0 20 2920.0 21 3778.0 22 4724.0 23 5537.0 24 6650.0 25 7585.0 26 8872.0 27 9832.0 28 10785.0 29 12826.0 30 15369.0 31 18940.0 32 24404.0 33 33125.0 34 45040.0 35 63930.0 36 95124.0 37 157131.0 38 244562.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94021207647025 18.7208778355161 12.19211999769899 26.146790090314664 2 13.977132595031428 19.958216102963185 43.143250523795274 22.921400778210117 3 19.080744092268016 24.164081516344815 29.219641794547297 27.53553259683987 4 11.286237151208633 16.824184383670804 39.37727557165231 32.512302893468245 5 12.0757185621223 38.8649630628034 36.705300131914285 12.354018243160011 6 31.08914561806353 37.96725507079985 18.900210486031384 12.043388825105243 7 27.479394352277414 31.066072405223615 23.352627639873727 18.10190560262524 8 23.672773324506743 39.134418428387086 20.939048551047918 16.253759696058257 9 25.868619668182685 15.422820124302932 20.496470270263806 38.212089937250575 10 13.6454107019827 29.99083767128531 35.89826705197887 20.46548457475312 11 34.17009287229641 22.468614043018313 25.415629237911414 17.945663846773865 12 23.141731294733884 24.656928108874126 33.095510018596144 19.10583057779585 13 29.030811453992094 22.651590799017 25.841559885914506 22.4760378610764 14 19.36457937196993 23.305134405323116 28.142841083852943 29.187445138854006 15 21.982373059721123 33.39468082562006 22.997297361938244 21.625648752720576 16 20.540581266578485 28.75322600320029 29.95692284141817 20.74926988880305 17 20.4800618045789 29.805880045540217 29.04384680883633 20.670211341044556 18 20.236063691753913 28.30145357354269 31.622489431545713 19.83999330315768 19 23.178709734517508 27.34979154158763 27.625822531687355 21.845676192207506 20 22.70674677586009 28.33093356549692 29.061866174275764 19.900453484367226 21 21.613589887707633 27.711220865572045 27.465827961875604 23.209361284844714 22 21.82284796525221 29.887574339539608 27.93815332989714 20.351424365311043 23 20.795786548095563 29.334986886779024 28.668024430175837 21.201202134949572 24 22.756457401301823 27.429560242570357 29.396169148695723 20.417813207432093 25 21.552959184893748 28.436636730008015 29.048325181473555 20.962078903624686 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 209.0 1 209.0 2 301.0 3 393.0 4 393.0 5 393.0 6 603.5 7 814.0 8 814.0 9 814.0 10 1264.5 11 1715.0 12 1715.0 13 1715.0 14 2906.0 15 4097.0 16 4097.0 17 4097.0 18 7338.5 19 10580.0 20 10580.0 21 10580.0 22 16726.5 23 22873.0 24 22873.0 25 22873.0 26 33313.0 27 43753.0 28 43753.0 29 43753.0 30 58323.5 31 72894.0 32 72894.0 33 72894.0 34 82337.0 35 91780.0 36 91780.0 37 91780.0 38 97055.0 39 102330.0 40 102330.0 41 102330.0 42 105761.5 43 109193.0 44 109193.0 45 109193.0 46 110371.5 47 111550.0 48 111550.0 49 111550.0 50 104160.5 51 96771.0 52 96771.0 53 96771.0 54 82933.0 55 69095.0 56 69095.0 57 69095.0 58 57993.5 59 46892.0 60 46892.0 61 46892.0 62 37238.5 63 27585.0 64 27585.0 65 27585.0 66 20986.5 67 14388.0 68 14388.0 69 14388.0 70 10234.0 71 6080.0 72 6080.0 73 6080.0 74 4157.5 75 2235.0 76 2235.0 77 2235.0 78 1521.0 79 807.0 80 807.0 81 807.0 82 523.0 83 239.0 84 239.0 85 239.0 86 150.0 87 61.0 88 61.0 89 61.0 90 37.5 91 14.0 92 14.0 93 14.0 94 9.0 95 4.0 96 4.0 97 4.0 98 4.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2320779284444322 2 0.13235974589798374 3 0.1360662970477918 4 0.06910924401900145 5 0.024869762553550695 6 0.02355453472619946 7 0.3114698627499979 8 0.3004697754666966 9 0.02080451290537414 10 0.03885900398992296 11 0.018532755749040182 12 0.019369718911900063 13 0.016978395589443262 14 0.018532755749040182 15 0.016380564758829066 16 0.0218806084004797 17 0.0206849467392513 18 0.016978395589443262 19 0.026184990380901937 20 0.01972841741026858 21 0.01757622642005746 22 0.01913058657965438 23 0.0212827775698655 24 0.017456660253934622 25 0.01757622642005746 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 836357.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.63794250829427 #Duplication Level Percentage of deduplicated Percentage of total 1 93.36002455957326 78.0844036668653 2 5.042095066957047 8.434209146630153 3 0.7541449720873729 1.8922540145508864 4 0.27121718095455155 0.907361879517537 5 0.14691556430006744 0.6143857760251326 6 0.08237346571525392 0.4133728313820815 7 0.0626600461946285 0.36685301388353814 8 0.04226912716479011 0.28282422621475956 9 0.029427500176087647 0.22151300111013947 >10 0.17298411268911168 2.68591812681684 >50 0.018446509331089326 1.0525508529480652 >100 0.014582567945144408 2.591768895446241 >500 0.0020015288381124647 1.0408926268706578 >1k 8.577980734767706E-4 1.4116919417386542 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2907 0.3475788449190956 No Hit CTGTAGGACGTGGAATATGGCAAGA 2316 0.27691524074049717 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2015 0.24092582473752236 No Hit GTCCTACAGTGTGCATTTCTCATTT 1690 0.2020668207475994 No Hit CTTTAGGACGTGAAATATGGCGAGG 1641 0.19620807860758027 No Hit CTGTAGGACCTGGAATATGGCGAGA 1237 0.14790334749395292 No Hit GTATCAACGCAGAGTACTTTTTTTT 930 0.11119653449424109 No Hit CTGAAGGACCTGGAATATGGCGAGA 860 0.1028269028656423 No Hit GTCCTTCAGTGTGCATTTCTCATTT 842 0.10067471187543119 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 370 0.0 15.181817 4 TAGTACG 40 0.0052003735 14.281201 4 TAGCGAG 40 0.0053407387 14.22399 18 GCCTCGA 65 5.5407665E-5 13.129838 16 TCGAACT 65 5.5407665E-5 13.129838 19 AGGACCT 710 0.0 12.600466 5 GGACCTG 695 0.0 12.324656 6 CTGTAGG 1140 0.0 12.30045 1 GGCGAGG 415 0.0 12.110385 19 TGTAGGA 1145 0.0 11.989593 2 GTAGGAC 1125 0.0 11.778327 3 GTATTAG 65 7.744824E-4 11.740461 1 ATCCCGT 75 2.1035699E-4 11.381234 10 GTAGTAC 110 4.740832E-7 11.266058 3 CTAGAGT 145 1.1696102E-9 11.162318 4 GTCCTAC 1400 0.0 10.969995 1 GTCTTAT 105 3.2930493E-6 10.901857 1 CCACCTT 235 0.0 10.894972 13 GTAAGAC 70 0.0014478773 10.8946495 3 AGTACCT 70 0.0014676041 10.876998 5 >>END_MODULE