FastQCFastQC Report
Thu 26 May 2016
SRR1926739_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926739_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836357
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT28670.34279619827418195No Hit
CTGTAGGACGTGGAATATGGCAAGA25570.3057306867761016No Hit
GTCCTAAAGTGTGTATTTCTCATTT21000.2510889488579638No Hit
CTTTAGGACGTGAAATATGGCGAGG19290.23064313445095813No Hit
GTCCTACAGTGTGCATTTCTCATTT17710.21175168020354942No Hit
GTATCAACGCAGAGTACTTTTTTTT15650.1871210499822444No Hit
CTGTAGGACCTGGAATATGGCGAGA15370.1837731973308049No Hit
CTGAAGGACCTGGAATATGGCGAGA11400.13630542938003748No Hit
GGTATCAACGCAGAGTACTTTTTTT8720.10426169685911638No Hit
TATCAACGCAGAGTACTTTTTTTTT8600.1028269028656423No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC400.005191814614.2847484
ATCCCGT759.810756E-713.91386810
TAGGACC5000.012.5705784
GTCCTAA4550.012.3620731
GACAGCC1001.4589205E-712.33717257
CTTACCC853.823652E-612.3263163
CCTTATA1251.3278623E-912.2035672
TGTAGGA15400.012.1961372
GTAGGAC15000.011.937853
GACGTGG7400.011.9267877
GTGTAGC2100.011.8033361
ATTATAC1052.6232738E-711.7927083
CTGTAGG15550.011.6486041
CGTGGAA7850.011.4821279
GTCTTAA1001.8460778E-611.4401561
ACGTGGA7850.011.2403988
AGGACGT12700.011.1728485
CTTATAC1451.1605152E-911.1671013
GGACGTG12400.011.135966
GTCCTAC11050.011.1295641