FastQCFastQC Report
Thu 26 May 2016
SRR1926738_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926738_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639
Sequences flagged as poor quality0
Sequence length25
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTT40.24405125076266015No Hit
GGTATCAACGCAGAGTACTTTTTTT40.24405125076266015No Hit
GTATCAACGCAGAGTACTTTTTTTT30.18303843807199513No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA20.12202562538133008No Hit
CCCATGTACTCTGCGTTGATACCAC20.12202562538133008No Hit
GTACATGGGAAGCAGTGGTATCAAC20.12202562538133008No Hit
CTGTAGGACGTGGAATATGGCAAGA20.12202562538133008No Hit
GTCCTAAAGTGTGTATTTCTCATTT20.12202562538133008No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)