FastQCFastQC Report
Thu 26 May 2016
SRR1926738_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926738_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1639
Sequences flagged as poor quality0
Sequence length25
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA60.36607687614399026No Hit
GTATCAACGCAGAGTACTTTTTTTT40.24405125076266015No Hit
GTACATGGGAAGCAGTGGTATCAAC30.18303843807199513No Hit
CGTCCTAAAGTGTGTATTTCTCATT30.18303843807199513No Hit
CTTATACACATCTCCGAGCCCACGA30.18303843807199513No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA30.18303843807199513No Hit
GTCCACTGTAGGACGTGGAATATGG20.12202562538133008No Hit
CTCTCTCTCTCTCTCTCTCTCTCTC20.12202562538133008No Hit
CCACTTGACGACTTGAAAAATGACG20.12202562538133008No Hit
CTTTAGGACGTGAAATATGGCGAGG20.12202562538133008No Hit
CTGTAGGACGTGGAATATGGCAAGA20.12202562538133008No Hit
ATACACATCTCCGAGCCCACGAGAC20.12202562538133008No Hit
GTCCTACAGTGTGCATTTCTCATTT20.12202562538133008No Hit
CCATATTTCACGTCCTAAAGTGTGT20.12202562538133008No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20.12202562538133008No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)