##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926737_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11880 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 10.026010101010101 2.0 2.0 22.0 2.0 28.0 2 10.108838383838384 2.0 2.0 22.0 2.0 28.0 3 10.202356902356902 2.0 2.0 22.0 2.0 28.0 4 11.618602693602694 2.0 2.0 26.0 2.0 32.0 5 11.386616161616162 2.0 2.0 26.0 2.0 32.0 6 10.760690235690236 2.0 2.0 23.0 2.0 32.0 7 10.454713804713805 2.0 2.0 22.0 2.0 32.0 8 10.327441077441078 2.0 2.0 21.0 2.0 32.0 9 10.643518518518519 2.0 2.0 24.0 2.0 32.0 10 10.57348484848485 2.0 2.0 23.0 2.0 33.0 11 10.393518518518519 2.0 2.0 22.0 2.0 33.0 12 10.273905723905724 2.0 2.0 22.0 2.0 33.0 13 10.075925925925926 2.0 2.0 19.0 2.0 33.0 14 10.01043771043771 2.0 2.0 18.0 2.0 33.0 15 9.907575757575758 2.0 2.0 17.0 2.0 33.0 16 9.756144781144782 2.0 2.0 17.0 2.0 33.0 17 9.72979797979798 2.0 2.0 17.0 2.0 33.0 18 9.579208754208754 2.0 2.0 17.0 2.0 33.0 19 9.268855218855219 2.0 2.0 11.0 2.0 32.0 20 9.151851851851852 2.0 2.0 10.0 2.0 32.0 21 9.04983164983165 2.0 2.0 10.0 2.0 32.0 22 8.913215488215489 2.0 2.0 2.0 2.0 33.0 23 8.722306397306397 2.0 2.0 2.0 2.0 33.0 24 8.167424242424243 2.0 2.0 2.0 2.0 32.0 25 8.179377104377105 2.0 2.0 2.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.075324857744736 1101 2 -1.0133936692001502 1101 3 -0.2981462954743481 1101 4 0.8686951995013512 1101 5 1.3149272794278648 1101 6 0.016197570304351316 1101 7 0.32689757788765306 1101 8 -0.13518242071653042 1101 9 -0.5304505285496628 1101 10 -0.15007060315296528 1101 11 1.1898692535441295 1101 12 1.1106163039059034 1101 13 2.3731889436943536 1101 14 1.66368335582691 1101 15 1.4008052043551231 1101 16 2.8268046714296364 1101 17 3.652555762037089 1101 18 1.6486166826230253 1101 19 0.9068287293069073 1101 20 2.2635933656390943 1101 21 4.357650898111798 1101 22 2.9687580543916248 1101 23 3.6571365197369214 1101 24 3.536881196489867 1101 25 4.232249620276434 1102 1 1.135475233684586 1102 2 0.8938745012259162 1102 3 1.8346857847261528 1102 4 2.3599232696767896 1102 5 3.4101653746659597 1102 6 2.244267745742949 1102 7 0.6627371768851482 1102 8 2.513940386301014 1102 9 3.652506864934047 1102 10 2.601809096095156 1102 11 3.0695685016644294 1102 12 4.529162669820691 1102 13 3.5436150088572607 1102 14 2.485738493671521 1102 15 3.0825094650067513 1102 16 2.267907428321868 1102 17 2.675112153014533 1102 18 0.5007470084375623 1102 19 2.54843273933197 1102 20 2.5768765736591437 1102 21 1.1045180660315985 1102 22 1.62289339273749 1102 23 2.404003687656722 1102 24 0.29126716140214803 1102 25 0.9264852092488649 1103 1 2.8432674414767938 1103 2 2.831103938455353 1103 3 3.505681455721824 1103 4 0.9356808454343639 1103 5 1.8603818248824098 1103 6 2.5018435033187068 1103 7 1.898667912815883 1103 8 5.0896979620585885 1103 9 2.2542384666656474 1103 10 4.790120784406843 1103 11 5.108529540625469 1103 12 5.36898950964753 1103 13 4.617208082450334 1103 14 3.522535030468058 1103 15 2.6950635775608642 1103 16 4.322019982434421 1103 17 3.707579685482065 1103 18 3.9596214673120205 1103 19 3.6891253800246098 1103 20 4.05306704984962 1103 21 3.972483433996967 1103 22 2.609906379750477 1103 23 3.3628781465311803 1103 24 3.7306611007960875 1103 25 2.885359668123323 1104 1 -3.1502390520296997 1104 2 -3.153744546393132 1104 3 -4.117695167654799 1104 4 -3.8781719684184495 1104 5 -3.7326917681911826 1104 6 -3.755732254257052 1104 7 -4.908691394543424 1104 8 -3.724154851794225 1104 9 -4.252255039827859 1104 10 -3.398190903904845 1104 11 -3.406621974526046 1104 12 -2.7089325682745473 1104 13 -3.8373373720951207 1104 14 -2.7523567444237163 1104 15 -2.060347677850391 1104 16 -1.732092571678133 1104 17 -2.0867926088902298 1104 18 -2.0230625153719615 1104 19 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-1.486060825646387 1107 17 -1.6661576882553089 1107 18 0.0404295481201018 1107 19 -2.967440276541046 1107 20 -2.780266283483713 1107 21 -1.3002438387303066 1107 22 -2.3294875596434634 1107 23 -1.7229804393274053 1107 24 -2.708732838597853 1107 25 -1.8671655844019295 1108 1 -1.5283903125339018 1108 2 -1.8988425856088185 1108 3 -2.6387035710161433 1108 4 -1.2675277107153686 1108 5 -2.155660955866253 1108 6 -2.089065587590385 1108 7 -2.5585513385210152 1108 8 -0.8782164764440843 1108 9 -2.3446920146177748 1108 10 0.038783886011122704 1108 11 0.3972995941014048 1108 12 0.9465296165994026 1108 13 0.2803096867284083 1108 14 0.7070270090776845 1108 15 1.1469352353148743 1108 16 0.6348542070333512 1108 17 0.330574337888482 1108 18 1.066573338969775 1108 19 1.1142590698641825 1108 20 -2.8881094207386155 1108 21 -0.9178908975538356 1108 22 -0.6628208929767965 1108 23 -0.20664057004635872 1108 24 0.9579338280688141 1108 25 -0.21357081316009907 1109 1 2.471609687466099 1109 2 3.6893927085088283 1109 3 2.1848258407485623 1109 4 3.9089428775199266 1109 5 3.197280220604336 1109 6 2.028581471233144 1109 7 0.14733101442016228 1109 8 -0.5252752999734955 1109 9 -1.109397896970716 1109 10 -1.6082749375182903 1109 11 -1.0144651117809484 1109 12 -0.9946468539888329 1109 13 -1.8373373720951207 1109 14 0.5893799502541555 1109 15 -2.0883588823321846 1109 16 -1.247498734143118 1109 17 -1.9635433091703414 1109 18 -1.1098972492655186 1109 19 -0.5327997536652287 1109 20 -1.7116388325033212 1109 21 -3.5649497210832477 1109 22 -3.0745855988591497 1109 23 -2.618405275928712 1109 24 -4.159713230754715 1109 25 -3.9194531661012757 1110 1 2.611665709875062 1110 2 1.2748268821782975 1110 3 -0.022457072416703028 1110 4 3.312304222057742 1110 5 1.9815939460945309 1110 6 2.577601079076281 1110 7 2.5674990816470515 1110 8 2.2282261005867277 1110 9 3.3667925792197604 1110 10 2.601809096095156 1110 11 3.2124256445215735 1110 12 4.576781717439738 1110 13 2.4007578660001165 1110 14 1.72383373176676 1110 15 2.70155708405437 1110 16 1.2202883807028204 1110 17 2.5798740577764363 1110 18 3.595985103675657 1110 19 2.0722422631414936 1110 20 2.8149718117543827 1110 21 3.818803780317312 1110 22 2.718131487975585 1110 23 2.165908449561483 1110 24 2.148410018545004 1110 25 2.1169613997250547 1111 1 0.9291260273353785 1111 2 2.512922120273535 1111 3 1.652146102186471 1111 4 2.8599232696767896 1111 5 0.14826061276119873 1111 6 1.4664899679651704 1111 7 0.6309911451391166 1111 8 0.23616260852323556 1111 9 1.5572687696959502 1111 10 0.8002217945078538 1111 11 0.260044692140621 1111 12 0.28313092378894567 1111 13 -0.5595595943173421 1111 14 1.1841511920842205 1111 15 -0.33018894769166174 1111 16 1.6250502854647237 1111 17 1.5560645339669126 1111 18 0.8182073258978786 1111 19 2.4214486123478434 1111 20 1.219733716516286 1111 21 1.0886450501585827 1111 22 2.3371791070232035 1111 23 1.499241782894817 1111 24 2.62460049473548 1111 25 1.6328344155980705 1112 1 0.7624593606687124 1112 2 1.2906998980513134 1112 3 2.4299238799642495 1112 4 2.6932566030101217 1112 5 -0.2961838316832459 1112 6 2.910934412409615 1112 7 2.5754355895835594 1112 8 2.625051497412125 1112 9 3.05726876969595 1112 10 2.855777350063409 1112 11 1.1489335810295085 1112 12 2.505353146011167 1112 13 4.27377373901599 1112 14 1.5174845254175526 1112 15 -0.10796672546943853 1112 16 -0.4305052700908316 1112 17 -2.721713243810864 1112 18 -0.40401489632434284 1112 19 -0.4674402765410459 1112 20 -0.6135996168170461 1112 21 -0.7446882831747512 1112 22 0.2260679959120928 1112 23 0.499241782894817 1112 24 -0.31984394970896357 1112 25 -0.2560544732908179 1113 1 1.6402371384464907 1113 2 -0.5537445463931316 1113 3 -0.6700761200357519 1113 4 0.8265899363434563 1113 5 1.514927279427864 1113 6 2.377601079076282 1113 7 0.9865467006946709 1113 8 0.8472737196343463 1113 9 -1.0427312303040495 1113 10 -1.1220004277143687 1113 11 -1.6732886411927126 1113 12 0.07201981267783353 1113 13 -0.9040040387617871 1113 14 0.5619289698619987 1113 15 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8.649609032106317 1116 16 8.458383618798058 1116 17 6.480306958209336 1116 18 9.414166921857474 1116 19 6.18912538002461 1116 20 8.598521595304167 1116 21 11.01793797945151 1116 22 6.791724561568659 1116 23 9.499241782894817 1116 24 6.957933828068814 1116 25 9.748996031759686 1201 1 0.84023713844649 1201 2 1.1795887869402009 1201 3 1.1299238799642488 1201 4 2.4932566030101224 1201 5 -0.08507272057213555 1201 6 -2.3557322542570516 1201 7 0.11988003402800551 1201 8 -1.4860596136989859 1201 9 -1.976064563637383 1201 10 -0.45533376104770085 1201 11 1.8600446921406206 1201 12 1.9386864793445007 1201 13 1.8293292945715454 1201 14 3.0285956365286637 1201 15 3.625366607863894 1201 16 2.7917169521313916 1201 17 3.32273120063358 1201 18 2.7959851036756564 1201 19 1.177004167903398 1201 20 3.9864003831829535 1201 21 3.7330894946030266 1201 22 3.40384577368987 1201 23 2.4325751162281506 1201 24 0.024600494735480538 1201 25 0.35505663782029373 1202 1 -0.9749143767050246 1202 2 -0.07798697063555693 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2216 1 -3.126429528220176 2216 2 -3.3204112130597982 2216 3 -4.403409453369084 2216 4 -6.806743396989877 2216 5 -5.018406053905468 2216 6 -7.422398920923719 2216 7 -5.813453299305329 2216 8 -7.06939294703232 2216 9 -6.026064563637383 2216 10 -5.755333761047702 2216 11 -5.573288641192713 2216 12 -5.494646853988833 2216 13 -5.337337372095121 2216 14 -5.204737696804669 2216 15 -4.7746333921361055 2216 16 -5.458283047868609 2216 17 -5.5272687993664205 2216 18 -4.987348229657676 2216 19 -4.856329165429935 2216 20 -4.363599616817047 2216 21 -4.2169105053969735 2216 22 -4.329487559643463 2216 23 -3.91742488377185 2216 24 -3.542066171931186 2216 25 -3.47827669551304 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 6961.0 3 1.0 4 48.0 5 232.0 6 226.0 7 176.0 8 136.0 9 112.0 10 134.0 11 107.0 12 105.0 13 75.0 14 88.0 15 82.0 16 91.0 17 102.0 18 118.0 19 132.0 20 171.0 21 205.0 22 214.0 23 204.0 24 216.0 25 232.0 26 178.0 27 198.0 28 154.0 29 191.0 30 193.0 31 195.0 32 150.0 33 151.0 34 110.0 35 92.0 36 62.0 37 27.0 38 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.052755772796225 18.97016686330693 11.461318051575931 30.515759312320917 2 13.814016172506738 19.213274932614553 39.43733153638814 27.535377358490564 3 17.611387180998904 24.24829444959151 29.428114208708834 28.71220416070075 4 12.13653603034134 19.511167298777917 32.069110830172775 36.283185840707965 5 12.58423180592992 36.89353099730458 35.099393530997304 15.422843665768193 6 31.893212059962945 35.70827017011959 17.56779518275223 14.830722587165235 7 26.929240111326642 33.50763262207978 21.202665092350507 18.360462174243064 8 25.021075703928513 36.86562131175181 19.895464508514586 18.21783847580509 9 24.943157894736842 15.898947368421052 18.80421052631579 40.35368421052631 10 14.93894736842105 29.810526315789478 30.021052631578947 25.229473684210525 11 33.17615358706635 24.36847423374874 22.473896934994947 19.981475244189966 12 23.90535533849781 25.370495116200743 28.182889861906368 22.541259683395083 13 29.092286965308183 22.398113843044793 23.84641293364769 24.663186257999325 14 21.311889525092624 21.463455708992925 26.557763556753116 30.666891209161335 15 24.49477938699899 28.469181542606936 21.6992253283934 25.336813742000675 16 21.54286676772781 28.431867946774464 25.16422435573522 24.861040929762506 17 21.96025597844392 27.95554058605591 25.0 25.084203435500168 18 21.387672617042774 27.585045469855167 26.953519703603906 24.07376220949815 19 24.389422267138286 27.067542529897253 27.13491662455786 21.4081185784066 20 23.231727854496466 27.37453688110475 25.732569888851465 23.66116537554732 21 23.627484001347256 26.524082182553048 26.734590771303466 23.11384304479623 22 22.785449646345572 26.70932974065342 26.717750084203434 23.787470528797574 23 21.362411586392724 27.02930279555406 26.355675311552712 25.252610306500507 24 24.882115190299764 25.13472549680027 26.15358706635231 23.82957224654766 25 21.379252273492757 26.675648366453352 26.717750084203434 25.227349275850454 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 5.0 2 6.5 3 8.0 4 8.0 5 8.0 6 17.0 7 26.0 8 26.0 9 26.0 10 34.5 11 43.0 12 43.0 13 43.0 14 45.0 15 47.0 16 47.0 17 47.0 18 73.5 19 100.0 20 100.0 21 100.0 22 159.5 23 219.0 24 219.0 25 219.0 26 301.0 27 383.0 28 383.0 29 383.0 30 506.5 31 630.0 32 630.0 33 630.0 34 768.0 35 906.0 36 906.0 37 906.0 38 1076.0 39 1246.0 40 1246.0 41 1246.0 42 1350.0 43 1454.0 44 1454.0 45 1454.0 46 1518.0 47 1582.0 48 1582.0 49 1582.0 50 1573.5 51 1565.0 52 1565.0 53 1565.0 54 1444.0 55 1323.0 56 1323.0 57 1323.0 58 1153.5 59 984.0 60 984.0 61 984.0 62 823.0 63 662.0 64 662.0 65 662.0 66 534.5 67 407.0 68 407.0 69 407.0 70 289.5 71 172.0 72 172.0 73 172.0 74 122.0 75 72.0 76 72.0 77 72.0 78 49.5 79 27.0 80 27.0 81 27.0 82 20.5 83 14.0 84 14.0 85 14.0 86 9.5 87 5.0 88 5.0 89 5.0 90 2.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11784511784511784 2 0.06734006734006734 3 0.05892255892255892 4 0.12626262626262627 5 0.06734006734006734 6 0.050505050505050504 7 0.1936026936026936 8 0.15151515151515152 9 0.04208754208754208 10 0.04208754208754208 11 0.03367003367003367 12 0.03367003367003367 13 0.03367003367003367 14 0.03367003367003367 15 0.03367003367003367 16 0.050505050505050504 17 0.03367003367003367 18 0.03367003367003367 19 0.050505050505050504 20 0.03367003367003367 21 0.03367003367003367 22 0.03367003367003367 23 0.03367003367003367 24 0.03367003367003367 25 0.03367003367003367 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 11880.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.4023569023569 #Duplication Level Percentage of deduplicated Percentage of total 1 99.80523329663816 99.20875420875421 2 0.09314929291218561 0.1851851851851852 3 0.0423405876873571 0.12626262626262627 4 0.0 0.0 5 0.01693623507494284 0.08417508417508417 6 0.0 0.0 7 0.0 0.0 8 0.01693623507494284 0.13468013468013468 9 0.00846811753747142 0.07575757575757576 >10 0.01693623507494284 0.1851851851851852 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 12 0.10101010101010101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE