##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926737_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11880 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.060606060606062 26.0 10.0 30.0 2.0 31.0 2 20.90084175084175 26.0 10.0 30.0 2.0 31.0 3 21.451094276094278 27.0 15.0 30.0 2.0 31.0 4 24.542676767676767 31.0 17.0 35.0 2.0 35.0 5 23.092171717171716 30.0 7.0 35.0 2.0 35.0 6 22.352441077441078 30.0 2.0 35.0 2.0 35.0 7 21.881481481481483 29.0 2.0 35.0 2.0 35.0 8 21.522979797979797 29.0 2.0 35.0 2.0 35.0 9 22.032239057239057 29.0 2.0 35.0 2.0 39.0 10 21.725757575757576 28.0 2.0 35.0 2.0 39.0 11 21.64671717171717 28.0 2.0 35.0 2.0 39.0 12 21.291329966329965 27.0 2.0 35.0 2.0 39.0 13 21.114730639730638 27.0 2.0 35.0 2.0 38.0 14 21.321127946127945 27.0 2.0 36.0 2.0 39.0 15 21.171969696969697 27.0 2.0 36.0 2.0 39.0 16 21.10412457912458 27.0 2.0 36.0 2.0 39.0 17 20.933585858585857 27.0 2.0 36.0 2.0 39.0 18 20.796212121212122 27.0 2.0 36.0 2.0 39.0 19 20.713804713804713 27.0 2.0 36.0 2.0 39.0 20 20.523821548821548 27.0 2.0 36.0 2.0 39.0 21 20.313299663299663 27.0 2.0 36.0 2.0 39.0 22 20.106060606060606 27.0 2.0 36.0 2.0 39.0 23 19.862962962962964 26.0 2.0 36.0 2.0 39.0 24 19.636868686868688 25.0 2.0 36.0 2.0 39.0 25 19.492845117845118 25.0 2.0 36.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.672648337038051 1101 2 5.632099705225791 1101 3 6.358316865557555 1101 4 6.227381420498084 1101 5 6.661445192931549 1101 6 3.1968790588769593 1101 7 2.9971873103683535 1101 8 4.984192624797149 1101 9 2.9115735641620972 1101 10 1.7694981360614221 1101 11 2.300795680862471 1101 12 0.9697582444250585 1101 13 1.1396794011277684 1101 14 1.7600140283126713 1101 15 4.2985364011973 1101 16 4.608379813263486 1101 17 4.907213529676142 1101 18 5.161888999967676 1101 19 3.8568414503830226 1101 20 2.194998891306856 1101 21 3.955656667890011 1101 22 2.760510573111116 1101 23 3.6475397769703015 1101 24 1.498994125173276 1101 25 1.589613840468548 1102 1 1.8430744022009584 1102 2 0.9153077252759161 1102 3 0.8019258881139457 1102 4 -0.4493103088252255 1102 5 3.633876270625784 1102 6 4.146753745593749 1102 7 4.027262498338278 1102 8 2.4954708202858704 1102 9 2.302551007771118 1102 10 2.2481948779160597 1102 11 2.4210964327421713 1102 12 4.3632419536982425 1102 13 3.2424362933583453 1102 14 3.0407157826986335 1102 15 4.268461213227376 1102 16 2.2625151516093496 1102 17 3.2756345823077204 1102 18 0.25462082954161147 1102 19 1.5410519766988138 1102 20 0.4305878637379337 1102 21 2.622323334556679 1102 22 -0.12420120633750642 1102 23 0.9132039373713035 1102 24 2.4238061552484638 1102 25 3.2287115848294476 1103 1 -0.8106052514786946 1103 2 -1.6214888115206207 1103 3 -0.16560657941852241 1103 4 0.07666371714880071 1103 5 0.2810624178119312 1103 6 0.17056326940327438 1103 7 -1.459750488674711 1103 8 -1.6019317771167252 1103 9 -2.5286178233977132 1103 10 -0.5678224381012562 1103 11 -1.2087736971279597 1103 12 0.2831553736116632 1103 13 -1.417736866814817 1103 14 -0.019890277907425258 1103 15 -1.8354348906687257 1103 16 -3.159562770468572 1103 17 -1.406183599510463 1103 18 -1.273517698596919 1103 19 -1.4589480233011862 1103 20 -2.1040441708941025 1103 21 -1.3625251502918054 1103 22 0.38012779799149854 1103 23 0.3915589157262822 1103 24 0.8913386227809319 1103 25 -1.6544052982874327 1104 1 -0.2521636930371365 1104 2 3.2962601062282957 1104 3 2.420973507161566 1104 4 2.6935468340319204 1104 5 2.6814953182448313 1104 6 -0.5199129210729154 1104 7 -0.9251184540426749 1104 8 -2.1711958463807974 1104 9 -0.9831632779431665 1104 10 -1.9422813125601301 1104 11 -3.0074749958292593 1104 12 -2.2081866177303304 1104 13 -2.7099446590226073 1104 14 -2.006903264920414 1104 15 -2.8267768820107193 1104 16 1.1672770563712547 1104 17 2.6565869632601 1104 18 1.8260494009701809 1104 19 -1.9351384994916607 1104 20 -0.902745469595402 1104 21 0.7651804774138213 1104 22 1.2567511746148732 1104 23 -0.4201293959620287 1104 24 -0.9095271780848719 1104 25 -2.390336034218169 1105 1 -0.3878779787514226 1105 2 -0.044216084247892695 1105 3 -1.4337883976003383 1105 4 -2.6278817373966525 1105 5 -0.5280284912789774 1105 6 0.820563269403273 1105 7 -3.287023215947439 1105 8 -4.897386322571272 1105 9 -4.333163277943168 1105 10 -5.304186074464894 1105 11 -4.836046424400685 1105 12 -4.448662808206521 1105 13 -6.03137323045118 1105 14 -4.592617550634699 1105 15 -6.1672530724869095 1105 16 -4.523199134104935 1105 17 -6.12436541769228 1105 18 -4.614426789506009 1105 19 -5.008948023301187 1105 20 -5.88131689816683 1105 21 -5.84434333210999 1105 22 -4.878963111099411 1105 23 -4.112986538819172 1105 24 -3.5404795590372515 1105 25 -4.149859843741979 1106 1 3.1843442434708003 1106 2 4.272450582418774 1106 3 4.682878269066329 1106 4 2.733229373714458 1106 5 4.483082619832132 1106 6 6.448341047181053 1106 7 3.8129767840525624 1106 8 6.963724788539839 1106 9 6.850170055390166 1106 10 8.906925036646218 1106 11 8.563953575599314 1106 12 5.7124483029045905 1106 13 7.663071213993263 1106 14 7.7629380049208585 1106 15 5.649413594179759 1106 16 7.365689754783954 1106 17 5.66452347119661 1106 18 7.580017654938434 1106 19 6.041051976698814 1106 20 6.835349768499839 1106 21 9.288990001223343 1106 22 8.0821480000117 1106 23 3.2147912389586075 1106 24 3.93174266318497 1106 25 4.9668068229246884 1107 1 -1.982322423195864 1107 2 1.3280061379743273 1107 3 1.7384338246218825 1107 4 2.5110071514922367 1107 5 0.316415953165464 1107 6 -2.3294367305967256 1107 7 0.9240878951636731 1107 8 0.5748358996509495 1107 9 1.1279478331679442 1107 10 1.462480592201775 1107 11 3.1750646867104244 1107 12 3.823559414015701 1107 13 1.3852934362154876 1107 14 2.0407157826986335 1107 15 2.538302483068648 1107 16 2.976800865895065 1107 17 -0.5021431954700581 1107 18 1.9133509882717696 1107 19 2.763274198921035 1107 20 0.7242386573887281 1107 21 3.8445455567789004 1107 22 0.4710368889005885 1107 23 0.8814579056252718 1107 24 0.2650759965183056 1107 25 0.18902904514690988 1108 1 5.462122021248579 1108 2 5.988136856928577 1108 3 5.2220939553408385 1108 4 4.180941792015112 1108 5 5.283736214603373 1108 6 5.082327975285626 1108 7 2.1953297252290334 1108 8 1.7643783833110795 1108 9 1.193307310292127 1108 10 1.0016962784762846 1108 11 0.9168947520699007 1108 12 2.248396015322893 1108 13 -0.16372617162765124 1108 14 -0.17497049181116964 1108 15 2.335688103983678 1108 16 2.447389101189181 1108 17 1.9226934058371334 1108 18 1.870867328141049 1108 19 1.5410519766988138 1108 20 2.6098595724214064 1108 21 2.308597844360598 1108 22 3.029860418312353 1108 23 2.084072284710242 1108 24 3.4742263233156905 1108 25 2.3295519209639046 1109 1 -1.5378779787514212 1109 2 -0.6589219666008361 1109 3 -0.8955531034826905 1109 4 -0.5249405609260656 1109 5 -0.24567555010250786 1109 6 1.8470338576385679 1109 7 2.607094431111385 1109 8 2.823201912722844 1109 9 3.4874249573509495 1109 10 2.7075786314174586 1109 11 1.3286594579522557 1109 12 4.483690132969951 1109 13 4.542156181313526 1109 14 2.29561774348295 1109 15 3.2768645745719134 1109 16 4.329742042365652 1109 17 3.6285757587783074 1109 18 2.8120437987292846 1109 19 3.5410519766988138 1109 20 4.2569183959508194 1109 21 5.661539020831189 1109 22 4.5004486536064725 1109 23 4.6134840494161224 1109 24 4.180108676256864 1109 25 3.741316626846256 1110 1 1.5097410688676263 1110 2 1.1057839157521059 1110 3 1.944783030971088 1110 4 2.598308738793822 1110 5 -0.03279039604088396 1110 6 2.527706126546132 1110 7 1.9796434507192302 1110 8 -0.5045291797141296 1110 9 3.3501700553901657 1110 10 1.5815282112493918 1110 11 1.8496678613135984 1110 12 0.6489562394125272 1110 13 -0.3289922780702277 1110 14 0.564525306508159 1110 15 1.5541754989416638 1110 16 0.5006103897045904 1110 17 0.6089679156410526 1110 18 0.3022398771606589 1110 19 2.0172424528892883 1110 20 -0.4741740410239714 1110 21 -1.377676665443321 1110 22 -1.3622964444327437 1110 23 -0.6106055864382185 1110 24 -1.9571462257039194 1110 25 -1.152240796122932 1111 1 0.6287886879152467 1111 2 2.161339471307663 1111 3 -0.26156617537811755 1111 4 1.899896040381126 1111 5 0.9275270642765783 1111 6 -0.44054784170783634 1111 7 0.8129767840525624 1111 8 0.9081692329842852 1111 9 0.18350338872350136 1111 10 1.3513694810906642 1111 11 2.5639535755993137 1111 12 0.6568927473490369 1111 13 1.2186267695488198 1111 14 3.818493560476412 1111 15 4.649413594179759 1111 16 3.1434675325617327 1111 17 3.4423012489743883 1111 18 1.5244620993828804 1111 19 1.3188297544765923 1111 20 1.8909053240553924 1111 21 -0.32212110988776743 1111 22 -0.25118533332163295 1111 23 1.1036801278474968 1111 24 0.09840932985163775 1111 25 -0.5331931770753116 1112 1 -0.8156557565291997 1112 2 -2.7831049731367834 1112 3 -1.1504550642670068 1112 4 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5.623388674310725 1203 14 5.945477687460539 1203 15 6.316080260846423 1203 16 5.119658008752207 1203 17 7.085158391831531 1203 18 5.207001781922564 1203 19 6.779147214794051 1203 20 6.668683101833171 1203 21 5.908037620270964 1203 22 5.685322603186304 1203 23 6.484632508799876 1203 24 6.185710917153223 1203 25 4.8953782514961155 1204 1 0.21212202124857882 1204 2 -3.394216084247894 1204 3 -2.233788397600339 1204 4 0.6221182626033475 1204 5 1.4344715087210211 1204 6 -0.3294367305967256 1204 7 -2.0620232159474376 1204 8 -0.022386322571271933 1204 9 0.01683672205683351 1204 10 1.420813925535107 1204 11 1.6264535755993137 1204 12 0.9763371917934798 1204 13 2.1561267695488198 1204 14 0.7698824493653014 1204 15 1.670246927513091 1204 16 2.476800865895065 1204 17 3.0256345823077204 1204 18 2.260573210493991 1204 19 3.4160519766988138 1204 20 2.356183101833171 1204 21 1.0181566678900111 1204 22 3.7210368889005885 1204 23 2.999513461180829 1204 24 2.6470204409627485 1204 25 4.550140156258021 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2213 9 -5.2331632779431665 2213 10 -2.904186074464892 2213 11 -3.1860464244006863 2213 12 -4.3486628082065195 2213 13 -5.081373230451181 2213 14 -6.942617550634699 2213 15 -7.867253072486909 2213 16 -8.723199134104934 2213 17 -7.22436541769228 2213 18 -7.864426789506009 2213 19 -6.258948023301187 2213 20 -6.181316898166829 2213 21 -8.044343332109989 2213 22 -7.578963111099412 2213 23 -6.562986538819171 2213 24 -7.240479559037251 2213 25 -7.149859843741979 2214 1 -1.4664494073228482 2214 2 -3.465644655676467 2214 3 -1.412359826171766 2214 4 -2.9493103088252255 2214 5 0.3005429372924482 2214 6 -1.2580081591681527 2214 7 1.3129767840525624 2214 8 1.9954708202858704 2214 9 -1.4831632779431665 2214 10 2.36724249696368 2214 11 0.3496678613135984 2214 12 0.7441943346506221 2214 13 1.0043410552631045 2214 14 1.564525306508159 2214 15 3.197032641798806 2214 16 3.405372294466492 2214 17 2.061348868022005 2214 18 0.9927160676368487 2214 19 2.8267662624130985 2214 20 3.097254530404598 2214 21 1.527085239318584 2214 22 0.2567511746148732 2214 23 0.865584889752256 2214 24 0.7809490123913214 2214 25 -0.27128841517055235 2215 1 1.846737405863962 2215 2 2.7980916080597993 2215 3 0.8239039100919676 2215 4 2.429810570295654 2215 5 2.4104330471825612 2215 6 -0.17559057675057232 2215 7 -1.610100139024361 2215 8 0.5064598312748814 2215 9 1.2476059528260635 2215 10 1.8342754639966472 2215 11 3.102415114060854 2215 12 1.5244141148704031 2215 13 1.9493960003180497 2215 14 2.784305526288378 2215 15 2.6750546198207843 2215 16 2.746031635125835 2215 17 2.583326890000027 2215 18 2.404803979724761 2215 19 4.3872058228526605 2215 20 3.5917600249100943 2215 21 2.571041283274628 2215 22 3.7402676581313585 2215 23 2.1293211534885224 2215 24 1.7864435178858251 2215 25 2.4155247716426373 2216 1 -6.371211312084755 2216 2 -6.31088275091456 2216 3 -7.233788397600339 2216 4 -10.794548404063319 2216 5 -7.544695157945645 2216 6 -9.329436730596726 2216 7 -9.020356549280772 2216 8 -7.064052989237938 2216 9 -7.649829944609833 2216 10 -8.704186074464893 2216 11 -8.602713091067352 2216 12 -7.231996141539854 2216 13 -7.78137323045118 2216 14 -6.042617550634699 2216 15 -7.600586405820243 2216 16 -8.939865800771601 2216 17 -7.391032084358946 2216 18 -6.947760122839343 2216 19 -8.54228135663452 2216 20 -8.497983564833495 2216 21 -8.711009998776655 2216 22 -9.278963111099412 2216 23 -8.729653205485837 2216 24 -6.8738128923705855 2216 25 -5.6165265104086455 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 2360.0 3 0.0 4 29.0 5 306.0 6 425.0 7 287.0 8 195.0 9 146.0 10 132.0 11 106.0 12 127.0 13 95.0 14 79.0 15 88.0 16 83.0 17 95.0 18 102.0 19 118.0 20 123.0 21 173.0 22 201.0 23 221.0 24 271.0 25 243.0 26 337.0 27 305.0 28 316.0 29 381.0 30 434.0 31 484.0 32 535.0 33 579.0 34 577.0 35 609.0 36 572.0 37 482.0 38 264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.50370869858395 16.335131490222523 7.5944032366824015 34.566756574511125 2 14.012631578947369 19.52 36.22736842105263 30.240000000000002 3 17.49158249158249 23.24915824915825 26.910774410774412 32.34848484848485 4 11.363636363636363 16.54040404040404 34.562289562289564 37.533670033670035 5 14.04882154882155 39.23400673400673 31.885521885521882 14.831649831649832 6 32.56915658573725 37.45875983419339 16.36917350477963 13.60291007528974 7 30.524454920447848 33.02466537587339 18.511659230574963 17.9392204731038 8 27.06228956228956 36.69191919191919 17.55050505050505 18.695286195286194 9 27.264309764309765 16.843434343434343 17.55892255892256 38.333333333333336 10 15.016835016835017 30.042087542087543 31.607744107744107 23.333333333333332 11 35.294612794612796 23.627946127946128 21.405723905723907 19.67171717171717 12 23.03030303030303 25.833333333333336 28.92255892255892 22.213804713804713 13 28.58585858585859 23.173400673400675 23.779461279461277 24.46127946127946 14 21.734006734006734 21.809764309764308 24.924242424242422 31.531986531986533 15 23.1986531986532 30.218855218855218 21.742424242424242 24.84006734006734 16 23.661616161616163 28.383838383838384 24.31818181818182 23.636363636363637 17 23.392255892255893 28.51851851851852 24.671717171717173 23.41750841750842 18 22.28956228956229 28.703703703703702 25.168350168350166 23.83838383838384 19 23.223905723905723 28.14814814814815 25.1010101010101 23.526936026936028 20 23.813131313131315 27.415824915824917 25.27777777777778 23.493265993265993 21 23.90773634144288 27.317114235204983 25.313578584055897 23.461570839296236 22 24.183501683501685 26.776094276094277 25.757575757575758 23.282828282828284 23 23.400673400673398 27.13804713804714 24.36868686868687 25.092592592592595 24 22.39057239057239 27.744107744107744 26.094276094276093 23.77104377104377 25 23.368970452058253 26.534220052192946 25.675561915986194 24.421247579762607 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 18.0 1 18.0 2 21.5 3 25.0 4 25.0 5 25.0 6 26.0 7 27.0 8 27.0 9 27.0 10 32.0 11 37.0 12 37.0 13 37.0 14 43.5 15 50.0 16 50.0 17 50.0 18 70.0 19 90.0 20 90.0 21 90.0 22 143.0 23 196.0 24 196.0 25 196.0 26 286.5 27 377.0 28 377.0 29 377.0 30 464.0 31 551.0 32 551.0 33 551.0 34 666.5 35 782.0 36 782.0 37 782.0 38 933.5 39 1085.0 40 1085.0 41 1085.0 42 1230.5 43 1376.0 44 1376.0 45 1376.0 46 1472.5 47 1569.0 48 1569.0 49 1569.0 50 1576.5 51 1584.0 52 1584.0 53 1584.0 54 1492.0 55 1400.0 56 1400.0 57 1400.0 58 1288.0 59 1176.0 60 1176.0 61 1176.0 62 945.0 63 714.0 64 714.0 65 714.0 66 599.0 67 484.0 68 484.0 69 484.0 70 346.0 71 208.0 72 208.0 73 208.0 74 144.5 75 81.0 76 81.0 77 81.0 78 58.0 79 35.0 80 35.0 81 35.0 82 22.0 83 9.0 84 9.0 85 9.0 86 6.5 87 4.0 88 4.0 89 4.0 90 3.0 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13468013468013468 2 0.04208754208754208 3 0.0 4 0.0 5 0.0 6 0.4966329966329966 7 0.008417508417508417 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.008417508417508417 22 0.0 23 0.0 24 0.0 25 0.008417508417508417 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 11880.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.95622895622895 #Duplication Level Percentage of deduplicated Percentage of total 1 99.455597141885 98.41750841750842 2 0.3572643756379721 0.7070707070707071 3 0.059544062606328683 0.17676767676767677 4 0.03402517863218782 0.13468013468013468 5 0.05103776794828172 0.25252525252525254 6 0.0 0.0 7 0.02551888397414086 0.17676767676767677 8 0.01701258931609391 0.13468013468013468 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE