##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926736_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17722 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 9.544238799232593 2.0 2.0 18.0 2.0 28.0 2 9.541022457961855 2.0 2.0 16.0 2.0 28.0 3 9.64411465974495 2.0 2.0 19.0 2.0 28.0 4 10.918575781514502 2.0 2.0 25.0 2.0 32.0 5 10.666741902719783 2.0 2.0 23.0 2.0 32.0 6 10.112120528157092 2.0 2.0 19.0 2.0 32.0 7 9.891772937591695 2.0 2.0 17.0 2.0 32.0 8 9.741733438663807 2.0 2.0 17.0 2.0 32.0 9 10.055693488319603 2.0 2.0 18.0 2.0 32.0 10 10.019523755783771 2.0 2.0 18.0 2.0 32.0 11 9.890475115675432 2.0 2.0 18.0 2.0 32.0 12 9.733664371967047 2.0 2.0 17.0 2.0 32.0 13 9.5742015573863 2.0 2.0 17.0 2.0 32.0 14 9.56116691118384 2.0 2.0 17.0 2.0 33.0 15 9.333314524320054 2.0 2.0 15.0 2.0 32.0 16 9.244046947297145 2.0 2.0 11.0 2.0 32.0 17 9.241789865703645 2.0 2.0 11.0 2.0 32.0 18 9.086897641349735 2.0 2.0 10.0 2.0 32.0 19 8.73716284843697 2.0 2.0 8.0 2.0 32.0 20 8.72367678591581 2.0 2.0 2.0 2.0 32.0 21 8.58091637512696 2.0 2.0 2.0 2.0 32.0 22 8.437760975059248 2.0 2.0 2.0 2.0 32.0 23 8.246021893691458 2.0 2.0 2.0 2.0 32.0 24 7.72909378174021 2.0 2.0 2.0 2.0 32.0 25 7.7458526125719445 2.0 2.0 2.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.8408227766773173 1101 2 -1.8028169155447191 1101 3 -1.6233543464807116 1101 4 -1.245704214418085 1101 5 -0.5965859982900028 1101 6 0.4876704091182731 1101 7 -0.05224228920428686 1101 8 -0.34795253661753733 1101 9 -0.8157484066629532 1101 10 -2.360656626203843 1101 11 -1.299703240801163 1101 12 -0.16084324816928408 1101 13 0.027381135976938253 1101 14 1.1644334944028554 1101 15 0.5670735553053543 1101 16 0.7841767171839304 1101 17 1.6204495737988793 1101 18 1.7399402792781125 1101 19 0.06608693094639584 1101 20 -0.6925776587277968 1101 21 -0.1996478035021081 1101 22 0.8345254897096943 1101 23 0.9254344362328553 1101 24 -0.2394759606096768 1101 25 0.6217553667302163 1102 1 3.5767596409051006 1102 2 0.8317984690706659 1102 3 1.58818411505775 1102 4 2.932867214153344 1102 5 4.963853562149557 1102 6 6.320087991535853 1102 7 2.4257796888176912 1102 8 4.234465045800045 1102 9 3.1485373076227603 1102 10 3.3508818353346186 1102 11 2.8816154405175176 1102 12 3.6990468617208254 1102 13 3.4971613557571573 1102 14 3.9474005273698882 1102 15 3.484655972887772 1102 16 2.234726167733381 1102 17 3.895174848524155 1102 18 3.26741280675064 1102 19 2.958944073803538 1102 20 1.966763000612863 1102 21 2.391011537157233 1102 22 2.2054046105888148 1102 23 3.238621249419669 1102 24 1.0901943690606517 1102 25 2.6080191029939535 1103 1 2.0507856149310744 1103 2 1.2181621054343026 1103 3 2.095759872633508 1103 4 1.2845988158849462 1103 5 1.8556284539244494 1103 6 3.202122623570487 1103 7 2.261277524315526 1103 8 3.760439071774071 1103 9 2.305463714549168 1103 10 2.464518198970982 1103 11 3.329667388569467 1103 12 2.226103138777102 1103 13 2.2406678492636516 1103 14 3.474456804426165 1103 15 3.0041364923682927 1103 16 4.303990236997452 1103 17 3.512057965407271 1103 18 3.0336465729844058 1103 19 1.9903293551888197 1103 20 3.3563733902232524 1103 21 4.1756435717892675 1103 22 5.01168166686587 1103 23 4.234292245090664 1103 24 3.7395450184113015 1103 25 4.065811310786161 1104 1 0.23610030024575934 1104 2 0.3894907767629743 1104 3 -1.0079697310960967 1104 4 -0.514934983648855 1104 5 0.8264909247869205 1104 6 0.7953627168105797 1104 7 -1.2445499815119803 1104 8 0.9212782326132309 1104 9 0.9534823625678168 1104 10 1.4854972199500036 1104 11 0.2772198361219136 1104 12 0.6468490595230243 1104 13 -1.4726188640230617 1104 14 0.7028950328643937 1104 15 1.2209197091515094 1104 16 1.2841767171839304 1104 17 0.966603419952726 1104 18 0.04763258697042083 1104 19 0.7583946232540875 1104 20 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2.6903172396399153 2216 1 -1.9492143850689247 2216 2 -0.6909288036566075 2216 3 -1.3890886122149766 2216 4 -0.6093405780544483 2216 5 0.06774966604566224 2216 6 -3.4039379824901195 2216 7 -2.496298233260232 2216 8 -2.23956092822593 2216 9 -2.0884756793902257 2216 10 -3.626390891938109 2216 11 -3.170332611430534 2216 12 -3.486018073344109 2216 13 -4.668423059827258 2216 14 -5.01039168042232 2216 15 -4.3140453258135265 2216 16 -4.544494611487398 2216 17 -4.351578398229092 2216 18 -3.9208988815610484 2216 19 -4.161185796326332 2216 20 -3.7345357006858384 2216 21 -3.339507943362247 2216 22 -3.427712272528068 2216 23 -3.099041088242669 2216 24 -2.7150004361341527 2216 25 -2.5250977801229295 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 10914.0 3 0.0 4 70.0 5 294.0 6 327.0 7 223.0 8 169.0 9 186.0 10 163.0 11 146.0 12 128.0 13 116.0 14 123.0 15 111.0 16 140.0 17 161.0 18 154.0 19 197.0 20 224.0 21 299.0 22 286.0 23 274.0 24 302.0 25 288.0 26 259.0 27 293.0 28 246.0 29 277.0 30 247.0 31 249.0 32 255.0 33 197.0 34 136.0 35 133.0 36 79.0 37 44.0 38 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.56859971711457 19.683168316831683 10.817538896746818 30.93069306930693 2 14.450474898236093 20.2962460425147 39.31478968792402 25.93848937132519 3 18.136589778380824 24.57033016734509 29.01402080506558 28.279059249208505 4 12.301699316885903 19.2739795630328 33.76051487607972 34.663806244001584 5 12.721526131617564 37.43650524889943 34.738683824359406 15.103284795123603 6 31.39180494412462 35.44982503668586 17.665650750649057 15.492719268540467 7 26.490553230003393 32.93358977259871 21.676660255685032 18.899196741712863 8 24.28870411222354 35.901351886418915 20.906159850670285 18.903784150687255 9 24.76437722219087 16.25373892431853 18.80467294994074 40.177210903549856 10 14.88316965797494 30.031606276103396 31.341009143244158 23.744214922677504 11 33.805519498843054 23.923471979231334 21.46283650318867 20.808172018736947 12 24.030701506857046 25.588351487104237 27.732942039618493 22.648004966420228 13 28.201365765562393 22.281167108753316 24.25080422145719 25.2666629042271 14 21.554351506942094 21.9776498476126 25.990518117168982 30.47748052827633 15 24.77566454088831 29.335741294655453 22.179581240476328 23.70901292397991 16 22.485607856417204 27.71193136922903 25.747827068517893 24.054633705835872 17 21.667231064454224 27.95462241788012 25.601083643752116 24.777062873913533 18 21.17945823927765 27.39841986455982 26.681715575620768 24.740406320541762 19 24.562591714640476 25.59543966587651 27.362004740941416 22.479963878541597 20 23.398611659800213 26.897680456007677 26.49133698289971 23.2123709012924 21 24.030701506857046 26.508267960945876 27.03877193972572 22.42225859247136 22 22.427902251820083 26.604210169874147 27.01055364298211 23.957333935323664 23 21.27095208533213 28.13364185337773 27.01055364298211 23.58485241830803 24 25.332957110609485 25.423250564334086 26.51241534988713 22.7313769751693 25 21.564422371465657 27.24758733562842 26.367176477227833 24.820813815678086 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 19.0 1 19.0 2 21.0 3 23.0 4 23.0 5 23.0 6 38.0 7 53.0 8 53.0 9 53.0 10 55.5 11 58.0 12 58.0 13 58.0 14 71.0 15 84.0 16 84.0 17 84.0 18 120.5 19 157.0 20 157.0 21 157.0 22 258.5 23 360.0 24 360.0 25 360.0 26 482.5 27 605.0 28 605.0 29 605.0 30 789.0 31 973.0 32 973.0 33 973.0 34 1159.5 35 1346.0 36 1346.0 37 1346.0 38 1597.0 39 1848.0 40 1848.0 41 1848.0 42 2017.0 43 2186.0 44 2186.0 45 2186.0 46 2232.0 47 2278.0 48 2278.0 49 2278.0 50 2277.0 51 2276.0 52 2276.0 53 2276.0 54 2111.5 55 1947.0 56 1947.0 57 1947.0 58 1717.0 59 1487.0 60 1487.0 61 1487.0 62 1248.5 63 1010.0 64 1010.0 65 1010.0 66 791.0 67 572.0 68 572.0 69 572.0 70 422.5 71 273.0 72 273.0 73 273.0 74 193.0 75 113.0 76 113.0 77 113.0 78 76.0 79 39.0 80 39.0 81 39.0 82 25.0 83 11.0 84 11.0 85 11.0 86 6.5 87 2.0 88 2.0 89 2.0 90 1.5 91 1.0 92 1.0 93 1.0 94 1.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26520708723620356 2 0.19185193544746643 3 0.19185193544746643 4 0.05078433585374111 5 0.02257081593499605 6 0.02257081593499605 7 0.24827897528495657 8 0.24263627130120752 9 0.016928111951247035 10 0.02257081593499605 11 0.016928111951247035 12 0.016928111951247035 13 0.016928111951247035 14 0.02257081593499605 15 0.016928111951247035 16 0.02257081593499605 17 0.02257081593499605 18 0.011285407967498025 19 0.02257081593499605 20 0.016928111951247035 21 0.016928111951247035 22 0.016928111951247035 23 0.016928111951247035 24 0.011285407967498025 25 0.016928111951247035 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 17722.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.80938945942896 #Duplication Level Percentage of deduplicated Percentage of total 1 99.70304380103934 98.515968852274 2 0.17132088401576154 0.33856223902494076 3 0.03997487293701102 0.11849678365872926 4 0.022842784535434868 0.0902832637399842 5 0.0 0.0 6 0.011421392267717434 0.06771244780498814 7 0.005710696133858717 0.03949892788624309 8 0.005710696133858717 0.0451416318699921 9 0.0 0.0 >10 0.03997487293701102 0.7843358537411128 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 42 0.23699356731745852 No Hit GGTATCAACGCAGAGTACTTTTTTT 23 0.12978219162622728 No Hit TATCAACGCAGAGTACTTTTTTTTT 19 0.10721137569123124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE