##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926736_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17722 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.973140729037354 26.0 10.0 30.0 2.0 31.0 2 20.797088364744386 26.0 10.0 30.0 2.0 31.0 3 21.207087236203588 27.0 10.0 30.0 2.0 31.0 4 24.231858706692247 31.0 16.0 35.0 2.0 35.0 5 23.02922920663582 30.0 2.0 35.0 2.0 35.0 6 22.33861866606478 30.0 2.0 35.0 2.0 35.0 7 21.7918406500395 29.0 2.0 35.0 2.0 36.0 8 21.67689876989053 30.0 2.0 35.0 2.0 36.0 9 22.016589549712222 29.0 2.0 35.0 2.0 39.0 10 21.784730843019975 29.0 2.0 35.0 2.0 39.0 11 21.70686152804424 29.0 2.0 35.0 2.0 39.0 12 21.420889290147837 28.0 2.0 35.0 2.0 39.0 13 21.299289019298048 28.0 2.0 35.0 2.0 39.0 14 21.617932513260353 28.0 2.0 36.0 2.0 40.0 15 21.393747883986006 27.0 2.0 36.0 2.0 39.0 16 21.353966820900574 27.0 2.0 36.0 2.0 39.0 17 21.311985103261485 28.0 2.0 36.0 2.0 39.0 18 21.11556257758718 27.0 2.0 36.0 2.0 39.0 19 21.099932287552196 27.0 2.0 37.0 2.0 40.0 20 20.890418688635595 27.0 2.0 37.0 2.0 40.0 21 20.724184629274347 27.0 2.0 37.0 2.0 40.0 22 20.510721137569124 27.0 2.0 37.0 2.0 39.0 23 20.345164202685925 27.0 2.0 36.0 2.0 40.0 24 20.120133167814018 27.0 2.0 36.0 2.0 39.0 25 19.91327163976978 26.0 2.0 36.0 2.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.20969829220642922 1101 2 0.3687147688741206 1101 3 -0.6431459782046609 1101 4 0.690735114821166 1101 5 -0.6096998857476805 1101 6 -2.1515412289055185 1101 7 -1.2402035328778176 1101 8 -1.443125786154834 1101 9 0.11438366544988199 1101 10 0.3586207587940464 1101 11 0.877362020773699 1101 12 0.8610979976805098 1101 13 -0.9828052268663292 1101 14 -2.0443512695057073 1101 15 -2.420753289960654 1101 16 -2.0908688300173175 1101 17 -1.851312681188766 1101 18 -1.7718065397465885 1101 19 -3.2810740424412153 1101 20 -1.913254724724478 1101 21 -1.991377443257484 1101 22 -1.3797803532620776 1101 23 -1.629886361614826 1101 24 -1.8246996217916163 1101 25 -2.7906606219906536 1102 1 -0.27931269680455983 1102 2 0.34398949414884683 1102 3 0.41179907674039384 1102 4 0.3967790708651222 1102 5 -0.5657438417916367 1102 6 -0.7916511190154054 1102 7 0.14990635723206935 1102 8 -0.10521369824274629 1102 9 1.03471333577955 1102 10 -0.47104957087628563 1102 11 0.5889004823121589 1102 12 0.23472437130688206 1102 13 1.4567552126941088 1102 14 0.8704838953294569 1102 15 2.1534225342151707 1102 16 2.4613289721804854 1102 17 -0.3458181756942622 1102 18 0.10456708662703917 1102 19 0.2738710125038395 1102 20 1.5922397807700257 1102 21 0.3822489303688883 1102 22 0.2136262401445137 1102 23 1.4992345175060535 1102 24 -0.030743577835572466 1102 25 -0.7714298527598835 1103 1 -0.3940313115231753 1103 2 0.9468033469626995 1103 3 1.1866908516321715 1103 4 2.2571686812547327 1103 5 -0.19128496733275924 1103 6 1.6293445519802638 1103 7 1.9031531104788257 1103 8 0.8179464749174272 1103 9 2.256574807641023 1103 10 3.1674785676518553 1103 11 0.47526411867579554 1103 12 2.0009581375406498 1103 13 2.716495472434371 1103 14 2.702734977580537 1103 15 3.117708248500886 1103 16 2.685354946206459 1103 17 3.3165194866434007 1103 18 3.224696956756908 1103 19 4.306338544971371 1103 20 4.3303350188652665 1103 21 3.618179666299625 1103 22 5.04912407564235 1103 23 4.541442309713844 1103 24 3.0482607511687583 1103 25 1.7512974199673899 1104 1 4.248159830667966 1104 2 2.407176307335657 1104 3 3.626084791026109 1104 4 2.421504345590396 1104 5 2.121069345021553 1104 6 3.9638433864790983 1104 7 1.9521041594298723 1104 8 3.403028059999013 1104 9 3.2682298192960353 1104 10 1.897082297255583 1104 11 2.9542850976967756 1104 12 2.8995595361420463 1104 13 2.632579388518284 1104 14 2.0325718074173693 1104 15 4.194631325423963 1104 16 1.4860542469057592 1104 17 4.033302703426617 1104 18 2.3820396140995648 1104 19 1.642002880635708 1104 20 3.5098221983524454 1104 21 3.5470840952040525 1104 22 4.312527339045612 1104 23 3.485498253769787 1104 24 5.636838839746847 1104 25 5.13241630108627 1105 1 1.3278301603382978 1105 2 2.236846637005989 1105 3 1.1617990767403938 1105 4 3.0753504994365493 1105 5 2.9699704439226515 1105 6 1.5654917381274487 1105 7 2.007049214374927 1105 8 1.0019291589001114 1105 9 2.03471333577955 1105 10 2.707521857695145 1105 11 1.8389004823121589 1105 12 3.234724371306882 1105 13 2.5281837841226817 1105 14 3.6919124667580263 1105 15 2.5819939627866013 1105 16 4.0684718293233395 1105 17 2.0113246814485954 1105 18 3.0688528009127545 1105 19 3.0595852982181277 1105 20 2.556525495055741 1105 21 4.417963216083173 1105 22 3.3921976687159443 1105 23 4.320663088934623 1105 24 3.7192564221644275 1105 25 3.085713004382974 1106 1 2.1135444460525825 1106 2 1.0225609227202739 1106 3 1.0844181243594413 1106 4 0.033683832769881406 1106 5 -0.4526486036963959 1106 6 2.0535869762226895 1106 7 2.7213349286606423 1106 8 1.3709767779477282 1106 9 2.4573323833985974 1106 10 2.6599028100760975 1106 11 2.422233815645491 1106 12 1.9252005617830719 1106 13 3.087707593646492 1106 14 2.8883410381865993 1106 15 3.409374915167554 1106 16 4.723233734085245 1106 17 4.308943729067643 1106 18 0.4974242294841815 1106 19 0.3631567267895548 1106 20 0.28866835219859865 1106 21 0.6560584541784138 1106 22 -0.072088045569771 1106 23 0.9694726127441484 1106 24 1.0823516602596683 1106 25 2.5202368139067843 1107 1 -1.3164555539474172 1107 2 -1.0374390772797248 1107 3 -2.2339152089738903 1107 4 -1.4446495005634503 1107 5 0.10568472963693765 1107 6 -4.253079690443979 1107 7 -3.9586650713393574 1107 8 -4.8523565553856045 1107 9 -4.221000949934734 1107 10 -4.376763856590571 1107 11 -4.761099517687843 1107 12 -3.868132771550261 1107 13 -5.4689590730201765 1107 14 -4.876658961813401 1107 15 -3.602291751499113 1107 16 -3.8600995992480875 1107 17 -3.8543896042656876 1107 18 -4.182575770515818 1107 19 -4.031843273210445 1107 20 -4.976331647801402 1107 21 -3.420608212488254 1107 22 -5.27208804556977 1107 23 -5.14219405392252 1107 24 -4.869315006407 1107 25 -4.041429852759883 1108 1 1.4070227069221488 1108 2 1.7399522270680983 1108 3 0.8217369649391522 1108 4 2.1188287603061156 1108 5 0.7230760339847606 1108 6 1.3434420486864553 1108 7 1.28655231996499 1108 8 0.7441651837448298 1108 9 0.23812948484787455 1108 10 1.5258448390616053 1108 11 3.0128135257904205 1108 12 2.0049107067106107 1108 13 1.3884322313276485 1108 14 1.9807323425344237 1108 15 2.509012596326972 1108 16 2.661639531186694 1108 17 1.573436482690834 1108 18 2.9756850990494 1108 19 3.009895857224336 1108 20 3.881059656546423 1108 21 1.7828700483813122 1108 22 1.7539989109519674 1108 23 1.8299798591209573 1108 24 3.6385110805495238 1108 25 3.522048408109683 1109 1 -2.2660851835770472 1109 2 -0.7737353735760237 1109 3 -0.429470764529448 1109 4 0.37164679573284687 1109 5 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3.3546426537988268 1110 18 2.5296822940003096 1110 19 2.004285759047619 1110 20 2.083023190908275 1110 21 2.868424045576262 1110 22 2.121460341527005 1110 23 2.6294188493032884 1110 24 3.0971366064962247 1110 25 4.575344340788504 1111 1 1.4404675229756592 1111 2 1.7148686150279673 1111 3 0.2030078679491858 1111 4 1.4984274225134726 1111 5 3.3903001142523195 1111 6 4.656151078786792 1111 7 3.029027236352949 1111 8 2.0953357523067027 1111 9 2.8836144346806485 1111 10 4.47400537417866 1111 11 2.9158235592352355 1111 12 3.8226364592189697 1111 13 1.7095024654413642 1111 14 3.2633410381865993 1111 15 2.8100159408085794 1111 16 2.486054246905759 1111 17 2.917918088042004 1111 18 2.7666549987149516 1111 19 2.6035413421741715 1111 20 3.1636683521985987 1111 21 2.239391787511746 1111 22 2.658681185199459 1111 23 1.6393444076159405 1111 24 2.444531147439154 1111 25 2.63241630108627 1112 1 3.2153962979044337 1112 2 1.078116478275831 1112 3 1.570529235470552 1112 4 2.8160912401772897 1112 5 1.7649439888961957 1112 6 1.4980314206671324 1112 7 1.3880015953273102 1112 8 3.343199000169953 1112 9 0.7582583093245248 1112 10 2.669162069335357 1112 11 1.0611227045343803 1112 12 2.0548301914127016 1112 13 2.2451150010538967 1112 14 2.059637334482897 1112 15 3.302893433686073 1112 16 4.1769374377889505 1112 17 2.3737585438824595 1112 18 -0.20627947421952086 1112 19 -0.7340654954326666 1112 20 -0.18818349965325254 1112 21 0.0727251208450781 1112 22 -0.2202361937179198 1112 23 -0.8592310909595575 1112 24 -0.3389446360366293 1112 25 0.25634792501789505 1113 1 -0.021070938562800734 1113 2 -0.5159006157412662 1113 3 -1.2200690551277376 1113 4 -1.3861879621019106 1113 5 -1.4943152703630638 1113 6 -2.728464305828595 1113 7 -1.8940496867239744 1113 8 -1.981587324616374 1113 9 -1.6163855653193515 1113 10 -1.564456164282877 1113 11 -1.2380225946109178 1113 12 0.5149441515266631 1113 13 -1.4058821499432526 1113 14 -1.7366589618134007 1113 15 -1.8053686745760373 1113 16 -1.3600995992480875 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20 0.16366835219859865 2214 21 1.6322489303688883 2214 22 0.3921976687159443 2214 23 0.21352023179176527 2214 24 -0.7807435778355725 2214 25 -1.5571441384741682 2215 1 1.4939792286612779 2215 2 1.0008217922854925 2215 3 2.126084791026109 2215 4 1.727524412480026 2215 5 0.15785864268041294 2215 6 0.7347463965125449 2215 7 0.4604653634432516 2215 8 0.5702521402665717 2215 9 0.28160774571744085 2215 10 -0.34372037832969937 2215 11 1.4041178736165065 2215 12 1.6570846197540874 2215 13 -0.22026342084626194 2215 14 1.9807323425344237 2215 15 1.6829256398052337 2215 16 2.0529438790127834 2215 17 1.5299582218212677 2215 18 2.4104677077450525 2215 19 1.835982813746078 2215 20 2.7506248739377277 2215 21 3.5654787440334843 2215 22 2.6670423892128383 2215 23 1.8734581199905236 2215 24 2.3776415153321295 2215 25 1.956831016805335 2216 1 -0.5455464630383275 2216 2 -0.2501663500069995 2216 3 -3.4193697544284376 2216 4 -1.1064676823816342 2216 5 -0.6306789067267005 2216 6 -0.749443326807615 2216 7 -4.142301434975721 2216 8 -3.303265646294694 2216 9 -3.3191827681165513 2216 10 -2.1204002202269336 2216 11 -4.161099517687841 2216 12 -3.9081327715502603 2216 13 -3.4653227093838126 2216 14 -3.3730225981770374 2216 15 -3.382291751499114 2216 16 -2.087372326520814 2216 17 -2.410753240629326 2216 18 -2.684393952334002 2216 19 -1.3754796368468085 2216 20 -1.972695284165038 2216 21 -1.851517303397344 2216 22 -1.8902698637515876 2216 23 -1.0949213266497928 2216 24 -3.3002240973160895 2216 25 -2.8396116709417 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 3826.0 3 0.0 4 77.0 5 430.0 6 550.0 7 365.0 8 281.0 9 201.0 10 162.0 11 162.0 12 144.0 13 110.0 14 121.0 15 98.0 16 112.0 17 127.0 18 139.0 19 138.0 20 174.0 21 239.0 22 262.0 23 320.0 24 374.0 25 354.0 26 386.0 27 418.0 28 470.0 29 523.0 30 634.0 31 681.0 32 789.0 33 868.0 34 927.0 35 936.0 36 972.0 37 910.0 38 442.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.76227886734867 18.052337082461992 7.364494432826542 32.820889617362795 2 13.53001017293998 20.718887758562225 36.58302249350062 29.168079574997176 3 17.243325619461533 24.462380764237736 27.007958457978216 31.286335158322515 4 11.736824286197946 16.8660422074258 33.83929579054283 37.55783771583342 5 14.09062693978895 39.42215450595339 32.24423000959314 14.242988544664522 6 32.668555240793204 37.903682719546744 16.039660056657222 13.388101983002834 7 29.154436667419283 33.72093023255814 19.016708060510272 18.107925039512303 8 27.040298002031832 36.860819505587536 17.40602776837115 18.692854724009482 9 26.707303307371035 17.236708432102947 17.71644655152952 38.3395417089965 10 15.170156329363959 30.582990010722956 31.412607934985044 22.834245724928042 11 34.89277652370203 24.881489841986458 21.478555304740407 18.747178329571106 12 23.321295564834667 26.515066019636606 28.535154045818757 21.628484369709966 13 28.192539924383496 23.170249985892443 23.790982450200325 24.84622763952373 14 22.023473648572395 23.118158221419705 24.46112176955197 30.39724636045593 15 23.339540657976414 31.16641273065854 21.561988601094747 23.932058010270303 16 23.881270808645112 28.914846791941763 23.644263867727556 23.55961853168557 17 22.418462927434827 29.116352556144903 24.91253808825189 23.552646428168377 18 22.60594774561255 29.15185373285932 25.331527566164436 22.910670955363692 19 24.077417898657036 27.891885791671367 25.20595869540684 22.824737614264755 20 24.05484708272204 27.931384719557613 25.589662566301776 22.424105631418577 21 24.298854466452234 26.91157383894814 25.0155183116077 23.77405338299193 22 23.77271188353459 27.293759169393972 25.211601399390588 23.72192754768085 23 23.9927773389008 26.949554226385285 25.014106759959372 24.043561674754542 24 23.575217244103374 27.508181920776437 25.301884663130576 23.614716171989617 25 23.896851371177068 26.35142760410789 25.284956551179327 24.46676447353572 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 38.0 1 38.0 2 40.5 3 43.0 4 43.0 5 43.0 6 45.5 7 48.0 8 48.0 9 48.0 10 55.5 11 63.0 12 63.0 13 63.0 14 78.0 15 93.0 16 93.0 17 93.0 18 147.5 19 202.0 20 202.0 21 202.0 22 267.5 23 333.0 24 333.0 25 333.0 26 424.5 27 516.0 28 516.0 29 516.0 30 681.0 31 846.0 32 846.0 33 846.0 34 1022.5 35 1199.0 36 1199.0 37 1199.0 38 1404.5 39 1610.0 40 1610.0 41 1610.0 42 1802.0 43 1994.0 44 1994.0 45 1994.0 46 2163.0 47 2332.0 48 2332.0 49 2332.0 50 2365.0 51 2398.0 52 2398.0 53 2398.0 54 2273.0 55 2148.0 56 2148.0 57 2148.0 58 1895.0 59 1642.0 60 1642.0 61 1642.0 62 1378.5 63 1115.0 64 1115.0 65 1115.0 66 873.0 67 631.0 68 631.0 69 631.0 70 463.5 71 296.0 72 296.0 73 296.0 74 211.5 75 127.0 76 127.0 77 127.0 78 80.0 79 33.0 80 33.0 81 33.0 82 23.0 83 13.0 84 13.0 85 13.0 86 7.0 87 1.0 88 1.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16363841552872138 2 0.15799571154497236 3 0.028213519918745066 4 0.0 5 0.005642703983749013 6 0.4062746868299289 7 0.03385622390249407 8 0.02257081593499605 9 0.02257081593499605 10 0.016928111951247035 11 0.011285407967498025 12 0.0 13 0.005642703983749013 14 0.0 15 0.005642703983749013 16 0.005642703983749013 17 0.0 18 0.005642703983749013 19 0.0 20 0.0 21 0.005642703983749013 22 0.0 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 17722.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.47082722040402 #Duplication Level Percentage of deduplicated Percentage of total 1 99.1977537103891 97.68084866267915 2 0.6074150478482608 1.1962532445547907 3 0.08022462896109105 0.23699356731745852 4 0.02292132256031173 0.0902832637399842 5 0.028651653200389662 0.1410675995937253 6 0.017190991920233798 0.10156867170748222 7 0.011460661280155864 0.07899785577248618 8 0.005730330640077932 0.0451416318699921 9 0.0 0.0 >10 0.028651653200389662 0.42884550276492495 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 22 0.12413948764247829 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE