##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926732_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7202 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.256595390169398 26.0 10.0 30.0 2.0 31.0 2 22.075118022771452 27.0 16.0 30.0 2.0 31.0 3 22.494307136906414 27.0 16.0 30.0 2.0 31.0 4 25.949458483754512 31.0 22.0 35.0 2.0 35.0 5 24.014856984171065 31.0 10.0 35.0 2.0 37.0 6 23.447375728964175 31.0 8.0 35.0 2.0 37.0 7 22.957650652596502 30.0 2.0 35.0 2.0 37.0 8 22.64745903915579 30.0 2.0 35.0 2.0 37.0 9 23.174812552068868 30.0 2.0 37.0 2.0 39.0 10 22.929464037767286 30.0 2.0 37.0 2.0 39.0 11 22.688975284643156 30.0 2.0 37.0 2.0 39.0 12 22.45279089141905 30.0 2.0 37.0 2.0 39.0 13 22.337961677311856 30.0 2.0 37.0 2.0 39.0 14 22.537489586226048 30.0 2.0 37.0 2.0 40.0 15 22.510969175229103 30.0 2.0 37.0 2.0 40.0 16 22.4225215217995 30.0 2.0 37.0 2.0 40.0 17 22.242988058872534 30.0 2.0 37.0 2.0 40.0 18 22.08400444321022 30.0 2.0 37.0 2.0 40.0 19 22.023604554290475 30.0 2.0 37.0 2.0 40.0 20 21.791863371285753 29.0 2.0 37.0 2.0 40.0 21 21.61691196889753 29.0 2.0 37.0 2.0 40.0 22 21.553179672313245 29.0 2.0 37.0 2.0 40.0 23 21.184809775062483 28.0 2.0 37.0 2.0 40.0 24 21.105942793668426 29.0 2.0 37.0 2.0 40.0 25 20.968897528464314 29.0 2.0 37.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -3.4197782252469757 1101 2 -2.530804330218384 1101 3 -3.511135283986846 1101 4 -1.249539046023422 1101 5 -1.3296797820235327 1101 6 -1.3796849851537374 1101 7 0.4737068270271365 1101 8 0.20368856404013513 1101 9 0.9668865205974448 1101 10 3.5129351551226584 1101 11 1.7756978915182025 1101 12 2.173470084602897 1101 13 3.150704720626596 1101 14 1.4874434593184631 1101 15 1.8506504129551082 1101 16 -1.0474293068043004 1101 17 -0.22335971320346104 1101 18 0.22150288947163688 1101 19 0.49942407852563875 1101 20 -1.1414717227217217 1101 21 0.4520670518717367 1101 22 -2.467960685148171 1101 23 -1.321286807810246 1101 24 -1.7765328638375522 1101 25 -1.4944348836927013 1102 1 1.3984035929348444 1102 2 0.7419229425088893 1102 3 2.034319261467701 1102 4 0.7504609539765781 1102 5 0.3066838543401005 1102 6 2.802133196664446 1102 7 2.56461591793623 1102 8 3.3855067458583186 1102 9 1.6941592478701715 1102 10 2.785662427849932 1102 11 4.6847888006091125 1102 12 5.809833720966534 1102 13 4.8779774478993225 1102 14 2.0328980047730063 1102 15 3.8506504129551082 1102 16 3.8616616022866097 1102 17 2.867549377705629 1102 18 1.2215028894716369 1102 19 3.4994240785256387 1102 20 5.767619186369188 1102 21 4.72479432459901 1102 22 4.9865847693972825 1102 23 4.951440464917027 1102 24 3.9507398634351745 1102 25 5.596474207216389 1103 1 0.985816180347431 1103 2 2.790873991459936 1103 3 3.2021514292998674 1103 4 2.7644469679625914 1103 5 4.180809728465977 1103 6 4.935000329531579 1103 7 2.7324480857683966 1103 8 4.902989263340835 1103 9 3.1137396674505915 1103 10 4.645802287989792 1103 11 4.356117471937782 1103 12 4.2364071475399605 1103 13 3.7381173080391825 1103 14 0.03989101176601295 1103 15 3.207293769598465 1103 16 6.057465798090806 1103 17 4.769647279803532 1103 18 3.2984259663947135 1103 19 4.072850651952212 1103 20 4.187199605949608 1103 21 4.16535376515845 1103 22 5.853717636530149 1103 23 5.042349555826117 1103 24 2.9507398634351745 1103 25 6.939131549873732 1104 1 2.645156839688088 1104 2 4.774390474976421 1104 3 4.339514066662506 1104 4 4.412798616314241 1104 5 4.274216321872569 1104 6 -1.4056590111277636 1104 7 1.15552500884532 1104 8 1.7491431094946819 1104 9 -1.6499965962856749 1104 10 1.090857233044737 1104 11 2.191282307102618 1104 12 -0.4174390063061928 1104 13 3.4688865388084125 1104 14 2.578352550227553 1104 15 2.7402608025654978 1104 16 3.134388875013883 1104 17 -0.8402428300865807 1104 18 -0.7070685390997902 1104 19 -0.059017479915919324 1104 20 -2.1479652292152274 1104 21 0.445573545378231 1104 22 1.2658055486180615 1104 23 -1.8862218727453097 1104 24 -2.263545850850541 1104 25 1.8237469344891153 1105 1 -1.283414588883339 1105 2 -5.106561905975962 1105 3 -2.7081049809565414 1105 4 -1.8252966217809998 1105 5 -1.761497963841716 1105 6 -6.167563773032523 1105 7 -2.59447499115468 1105 8 -4.417523557171986 1105 9 -3.4952346915237698 1105 10 -4.790095147907646 1105 11 -2.701574835754524 1105 12 -5.917439006306193 1105 13 -1.6144467945249197 1105 14 -4.004980783105779 1105 15 -4.831167768863075 1105 16 -3.865611124986117 1105 17 -3.9354809253246756 1105 18 -3.8618304438616953 1105 19 -4.856636527534967 1105 20 -3.588441419691417 1105 21 -4.661569311764627 1105 22 -3.210384927572413 1105 23 -2.6243171108405505 1105 24 -2.3825934698981577 1105 25 -2.6762530655108847 1106 1 4.671130865662114 1106 2 4.014650215236159 1106 3 2.397955625104064 1106 4 1.3868245903402148 1106 5 4.852138399794647 1106 6 6.165769560300809 1106 7 3.110070463390773 1106 8 5.294597654949229 1106 9 6.239613793324715 1106 10 3.4220260642135685 1106 11 4.502970618790929 1106 12 4.991651902784717 1106 13 4.787068356990229 1106 14 4.032898004773006 1106 15 3.668832231136925 1106 16 2.8616616022866097 1106 17 2.867549377705629 1106 18 5.039684707653453 1106 19 3.863060442162002 1106 20 4.676710095460098 1106 21 3.72479432459901 1106 22 4.168402951215466 1106 23 4.769622283098844 1106 24 5.496194408889721 1106 25 5.323746934489115 1107 1 1.9943631888944395 1107 2 -0.9954507948648512 1107 3 0.12522835237679075 1107 4 -1.4919632884476641 1107 5 -1.2892757416194947 1107 6 2.387991782523031 1107 7 0.9888583421786521 1107 8 3.082476442828014 1107 9 2.3103208640317874 1107 10 3.9876826298701324 1107 11 2.8262029420232544 1107 12 4.082560993693807 1107 13 1.746664316586191 1107 14 5.356130328005332 1107 15 3.1132766755813677 1107 16 3.134388875013883 1107 17 5.675630185786435 1107 18 4.66594733391608 1107 19 1.7822523613539225 1107 20 2.939336358086358 1107 21 2.5328751326798162 1107 22 2.622948405760919 1107 23 3.48679400027056 1107 24 4.617406530101842 1107 25 2.6570802678224474 1108 1 1.9438581383893876 1108 2 2.3782865788725225 1108 3 3.8525010796495174 1108 4 4.386824590340215 1108 5 3.488502036158284 1108 6 4.347587742118993 1108 7 1.4737068270271365 1108 8 3.840052200403772 1108 9 2.421431975142898 1108 10 1.240207882395385 1108 11 2.5938797097000226 1108 12 2.082560993693807 1108 13 3.332522902444776 1108 14 4.21471618659119 1108 15 0.39610495840965143 1108 16 1.7707525113775198 1108 17 4.776640286796539 1108 18 2.585139253108 1108 19 0.49942407852563875 1108 20 2.494891913641915 1108 21 5.997521597326283 1108 22 5.168402951215466 1108 23 5.133258646735207 1108 24 0.8598307725260845 1108 25 2.4146560253982052 1109 1 -0.8834145888833405 1109 2 0.2601047606907052 1109 3 -3.17477164762321 1109 4 -3.4586299551143327 1109 5 -1.2114979638417154 1109 6 -1.6342304396991914 1109 7 0.15552500884531995 1109 8 -2.8508568905053195 1109 9 0.2214319751428988 1109 10 4.576571518759021 1109 11 -0.7515748357545249 1109 12 1.6825609936938086 1109 13 -1.8311134611915882 1109 14 -4.221647449772448 1109 15 -0.9311677688630766 1109 16 -2.7656111249861155 1109 17 -5.168814258658006 1109 18 -4.678497110528362 1109 19 -2.473303194201634 1109 20 -0.2051080863580843 1109 21 -3.311569311764625 1109 22 -9.777051594239081 1109 23 -7.757650444173883 1109 24 -7.249260136564825 1109 25 -6.376253065510884 1110 1 -7.200081255550005 1110 2 -5.189895239309294 1110 3 -6.208104980956541 1110 4 -5.075296621781 1110 5 -3.928164630508384 1110 6 -3.917563773032523 1110 7 -3.677808324488012 1110 8 -2.167523557171986 1110 9 -2.9952346915237698 1110 10 -3.040095147907646 1110 11 -2.451574835754524 1110 12 -0.9174390063061928 1110 13 -0.7811134611915875 1110 14 -2.421647449772447 1110 15 -2.081167768863075 1110 16 -0.19894445831944907 1110 17 -0.5188142586580078 1110 18 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1.6229484057609191 2211 23 3.309016222492783 2211 24 3.550739863435176 2211 25 3.990413601155783 2212 1 -1.711986017454766 2212 2 0.41724761783356357 2212 3 -0.5176287904803516 2212 4 2.412798616314241 2212 5 1.3456448933011416 2212 6 3.3086267031579517 2212 7 2.5126678659881776 2212 8 2.8920002523518242 2212 9 3.2785748322857557 2212 10 3.1622858044733064 2212 11 3.976996592816903 2212 12 5.7254181365509496 2212 13 1.9688865388084125 2212 14 3.9354954073704107 2212 15 3.597403659708352 2212 16 5.277246017871025 2212 17 3.731185741341992 2212 18 4.5786457466144945 2212 19 4.655268234369792 2212 20 5.637749056499057 2212 21 2.0884306882353734 2212 22 3.7658055486180615 2212 23 5.470920984397544 2212 24 5.4507398634351745 2212 25 4.538032648774831 2213 1 -0.11674792221667118 2213 2 1.393438094024038 2213 3 1.0418950190434586 2213 4 0.7580367115523359 2213 5 -0.5114979638417161 2213 6 -6.417563773032523 2213 7 -6.427808324488012 2213 8 -9.500856890505318 2213 9 -8.161901358190436 2213 10 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5.722948405760921 2214 23 6.542349555826117 2214 24 3.050739863435176 2214 25 4.123746934489116 2215 1 -2.158414588883339 2215 2 -2.814895239309294 2215 3 -2.9997716476232092 2215 4 -3.283629955114332 2215 5 -7.511497963841716 2215 6 -5.959230439699191 2215 7 -4.84447499115468 2215 8 -4.875856890505318 2215 9 -6.078568024857102 2215 10 -4.873428481240978 2215 11 -4.076574835754524 2215 12 -3.417439006306193 2215 13 -5.5311134611915875 2215 14 -3.921647449772447 2215 15 -4.831167768863075 2215 16 -2.990611124986117 2215 17 -3.768814258658008 2215 18 -2.653497110528363 2215 19 -5.648303194201635 2215 20 -5.130108086358085 2215 21 -3.6615693117646266 2215 22 -2.377051594239081 2215 23 -1.7076504441738827 2215 24 -2.0492601365648255 2215 25 -2.8012530655108847 2216 1 -5.069128874597624 2216 2 -3.2970380964521517 2216 3 -7.589057361908923 2216 4 -7.587201383685759 2216 5 -9.654355106698858 2216 6 -3.977087582556333 2216 7 -3.2016178482975377 2216 8 -2.250856890505318 2216 9 -1.7214251677142443 2216 10 -5.409142766955263 2216 11 -5.380146264325951 2216 12 -5.34601043487762 2216 13 -5.316827746905872 2216 14 -5.135933164058162 2216 15 -5.902596340291648 2216 16 -4.579896839271832 2216 17 -4.483099972943723 2216 18 -4.349925681956936 2216 19 -4.48758890848735 2216 20 -4.505108086358085 2216 21 -4.625855026050342 2216 22 -4.091337308524796 2216 23 -4.100507587031025 2216 24 -3.4778315651362526 2216 25 -3.9619673512251694 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 1190.0 3 0.0 4 21.0 5 238.0 6 246.0 7 181.0 8 114.0 9 98.0 10 79.0 11 66.0 12 61.0 13 62.0 14 44.0 15 52.0 16 55.0 17 51.0 18 53.0 19 53.0 20 72.0 21 90.0 22 113.0 23 122.0 24 157.0 25 162.0 26 169.0 27 180.0 28 185.0 29 232.0 30 264.0 31 298.0 32 288.0 33 308.0 34 372.0 35 404.0 36 417.0 37 428.0 38 277.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.638463679376564 17.047035903145005 7.417200111327582 32.89730030615085 2 14.386890709623662 18.414109151506736 37.453131509512566 29.745868629357037 3 17.814495973340737 22.702027214662593 27.64509858372674 31.838378228269924 4 11.552346570397113 14.912524298805888 35.892807553457374 37.64232157733963 5 14.60705359622327 37.73951680088864 32.49097472924188 15.162454873646208 6 33.77492678845349 36.03402593780505 16.259935852740202 13.931111421001255 7 30.463760066648156 32.08830880311025 18.63371285753957 18.81421827270203 8 27.297972785337404 35.712302138294916 18.25881699527909 18.730908081088586 9 27.825603998889196 15.592890863648986 17.967231324632046 38.61427381282977 10 15.620660927520133 27.839489030824772 32.32435434601499 24.2154956956401 11 35.51791169119689 21.855040266592614 22.382671480144403 20.244376562066094 12 22.563176895306857 24.673701749514024 29.700083310191616 23.0630380449875 13 28.75590113857262 21.618994723687866 23.743404609830606 25.881699527908914 14 23.076923076923077 21.119133574007222 24.923632324354347 30.880311024715358 15 23.521244098861427 28.742016106637045 22.21605109691752 25.520688697584003 16 23.632324354346014 27.714523743404612 24.70147181338517 23.951680088864205 17 23.312968619827824 26.96473201888364 25.492918633712858 24.229380727575673 18 23.28519855595668 26.450985837267428 26.78422660372119 23.479589003054706 19 24.09053040821994 26.78422660372119 25.40960844209942 23.715634545959453 20 25.034712579838935 26.450985837267428 25.3124132185504 23.201888364343237 21 24.180555555555554 25.333333333333336 25.59722222222222 24.88888888888889 22 24.409886142738127 26.06220494307137 25.60399888919744 23.92391002499306 23 23.465703971119133 26.54818106081644 24.74312690919189 25.242988058872534 24 23.81282976950847 25.812274368231048 26.31213551791169 24.062760344348792 25 23.71892792667685 26.107485071517843 25.982502430218023 24.19108457158728 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 1.0 2 2.0 3 3.0 4 3.0 5 3.0 6 3.0 7 3.0 8 3.0 9 3.0 10 7.5 11 12.0 12 12.0 13 12.0 14 12.5 15 13.0 16 13.0 17 13.0 18 32.0 19 51.0 20 51.0 21 51.0 22 76.0 23 101.0 24 101.0 25 101.0 26 139.5 27 178.0 28 178.0 29 178.0 30 243.5 31 309.0 32 309.0 33 309.0 34 412.5 35 516.0 36 516.0 37 516.0 38 587.0 39 658.0 40 658.0 41 658.0 42 734.0 43 810.0 44 810.0 45 810.0 46 880.5 47 951.0 48 951.0 49 951.0 50 984.0 51 1017.0 52 1017.0 53 1017.0 54 955.5 55 894.0 56 894.0 57 894.0 58 817.5 59 741.0 60 741.0 61 741.0 62 598.5 63 456.0 64 456.0 65 456.0 66 360.0 67 264.0 68 264.0 69 264.0 70 201.0 71 138.0 72 138.0 73 138.0 74 95.5 75 53.0 76 53.0 77 53.0 78 39.5 79 26.0 80 26.0 81 26.0 82 15.0 83 4.0 84 4.0 85 4.0 86 3.5 87 3.0 88 3.0 89 3.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22216051096917525 2 0.013885031935573453 3 0.0 4 0.0 5 0.0 6 0.430435990002777 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.027770063871146906 22 0.0 23 0.0 24 0.0 25 0.013885031935573453 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 7202.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.38905859483476 #Duplication Level Percentage of deduplicated Percentage of total 1 99.59485889913383 98.98639266870313 2 0.2794076557697681 0.5554012774229381 3 0.09779267951941883 0.2915856706470425 4 0.013970382788488405 0.05554012774229381 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.013970382788488405 0.11108025548458762 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8 0.11108025548458762 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE