FastQCFastQC Report
Thu 26 May 2016
SRR1926719_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926719_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481705
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56480.16221937240518655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18050.010.6762681
GTATCAA36900.010.6257791
ATACCGA856.4831093E-410.0779476
GTATTCG1053.923101E-59.9942161
CCGATCA1356.6064604E-79.8345629
TAGGACA4600.09.52382854
TGGACCG2201.6370905E-119.5180615
TAGGACC3050.09.36774
GTATTAT3200.09.241811
GTATTAA3000.09.2219351
GGACCGT1451.6643644E-69.1898526
TCTATAC3150.08.7781013
GTCTTAC2502.3646862E-118.7767391
GTCCTAA3600.08.7449381
GTGTAAA6350.08.5634041
CTAGGAC3800.08.5311673
GTCTTAT2803.6379788E-128.5177971
CTAGTAC1701.5305741E-68.4130893
GTCTATG4450.08.3608411
GTATTAG2752.0008883E-118.325761
Produced by FastQC (version 0.11.5)