##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926719_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3481705 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.959584456466015 31.0 28.0 31.0 16.0 34.0 2 27.930121018294198 31.0 28.0 31.0 16.0 34.0 3 27.876764975780546 31.0 28.0 31.0 16.0 34.0 4 31.51112429111599 35.0 32.0 37.0 17.0 37.0 5 31.256459981532036 35.0 32.0 37.0 17.0 37.0 6 30.690937629695796 35.0 31.0 37.0 11.0 37.0 7 30.816614273753807 35.0 32.0 37.0 11.0 37.0 8 30.560342992872744 35.0 31.0 37.0 10.0 37.0 9 32.82550101171696 37.0 32.0 39.0 17.0 39.0 10 33.01457877677747 37.0 33.0 39.0 17.0 39.0 11 33.18993625249698 38.0 33.0 39.0 17.0 39.0 12 33.05709099421117 38.0 33.0 39.0 16.0 39.0 13 33.16545313287599 38.0 33.0 39.0 17.0 39.0 14 34.1887678594252 39.0 34.0 41.0 17.0 41.0 15 33.70990994354777 39.0 33.0 41.0 10.0 41.0 16 33.79824023000226 39.0 33.0 40.0 11.0 41.0 17 34.03335061413876 39.0 34.0 41.0 15.0 41.0 18 34.08011132476761 39.0 34.0 41.0 11.0 41.0 19 33.43178098087 39.0 33.0 40.0 10.0 41.0 20 33.69856176786948 39.0 33.0 41.0 8.0 41.0 21 33.69927607307339 39.0 34.0 41.0 2.0 41.0 22 33.72261004306798 39.0 34.0 41.0 2.0 41.0 23 33.714211284413814 39.0 34.0 41.0 2.0 41.0 24 32.37661605449055 38.0 32.0 40.0 2.0 41.0 25 32.96067731183429 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.2320990195194348 1101 2 -0.39452844610132587 1101 3 -1.1456598765384882 1101 4 -0.5822443910677677 1101 5 -0.4912749629057167 1101 6 -2.457489685362596 1101 7 -0.7498692162454965 1101 8 -0.4173349856258106 1101 9 -0.3490764916970406 1101 10 -9.196549815650666 1101 11 -3.976172721775086 1101 12 -1.5967210467764446 1101 13 -0.8772539276257305 1101 14 -0.7129639121569724 1101 15 -2.1369741823671298 1101 16 -0.48587477645524757 1101 17 -0.3559039967332822 1101 18 -0.27277852560327176 1101 19 -1.123664981137047 1101 20 -0.3131557312890294 1101 21 -0.26686633197221 1101 22 -0.14581015337155634 1101 23 -0.2106585750594192 1101 24 -0.36579982545475076 1101 25 -0.29263661072702263 1102 1 0.09195266578172934 1102 2 -0.13740272644496798 1102 3 -0.20886036504173688 1102 4 -0.1809539953578927 1102 5 -0.19353433613033388 1102 6 -0.9993880104442603 1102 7 -0.06578656985715625 1102 8 0.4072977723379232 1102 9 0.37486455517528583 1102 10 0.5392948414136285 1102 11 0.26601063971046557 1102 12 0.38495200676344865 1102 13 0.2184394768643756 1102 14 0.06897181243103745 1102 15 0.5669465955206618 1102 16 0.4916465174811364 1102 17 0.32308801740259696 1102 18 0.09231320663466391 1102 19 0.6062962009542687 1102 20 0.445339942583864 1102 21 0.3815537034253822 1102 22 0.2591629845868866 1102 23 -0.03721211791916801 1102 24 -3.6967677075545886 1102 25 -1.7687321894100378 1103 1 -0.20483806864525533 1103 2 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1110 8 1.1496161618260246 1110 9 0.7147702380604386 1110 10 0.7013025463917657 1110 11 0.39950382313190147 1110 12 0.5114124118323957 1110 13 0.42390849719245693 1110 14 0.21600726096019685 1110 15 0.873685396589849 1110 16 0.5655032245416223 1110 17 0.17579690375485058 1110 18 0.3287685241862164 1110 19 -2.145223948243281 1110 20 -0.2130373463602666 1110 21 -0.13966818460296082 1110 22 0.20154149602499416 1110 23 0.1959196247156214 1110 24 -0.7255635001354364 1110 25 -0.36474695789361533 1111 1 0.4210599841896183 1111 2 0.0486898537623901 1111 3 0.76328838851488 1111 4 0.6282205328822812 1111 5 0.6870750728077191 1111 6 0.866460284554261 1111 7 0.8613878329250397 1111 8 1.2691791129957224 1111 9 0.47079768706394276 1111 10 0.43246656120556537 1111 11 0.18081502038617003 1111 12 0.3123580541915132 1111 13 0.2182948536615683 1111 14 0.30265317024560545 1111 15 -0.7341701219102816 1111 16 -0.3911915385482274 1111 17 -0.050052434074977725 1111 18 0.18778142266255315 1111 19 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0.14726888773247637 2215 4 0.4229531154114312 2215 5 0.783287995730003 2215 6 1.2649867851351715 2215 7 0.7214498499243334 2215 8 -0.5439684619362133 2215 9 -0.05787332916892751 2215 10 -0.2540285577699919 2215 11 -0.2170243102261722 2215 12 -0.5207486574426383 2215 13 -0.1902595629733952 2215 14 -0.2055093528622649 2215 15 -0.050988552669153364 2215 16 0.10915019386052194 2215 17 -0.30599487132997893 2215 18 -1.0699234013266548 2215 19 0.12040815021654083 2215 20 -4.001927093892718 2215 21 -2.2900622455583104 2215 22 -1.3037011229344202 2215 23 -1.133076128110595 2215 24 -1.1271455222585232 2215 25 -0.8827206337938023 2216 1 -0.5098551414200045 2216 2 -1.0226707181598584 2216 3 -0.3773719692401798 2216 4 -0.5016586177605511 2216 5 -0.3176792065170204 2216 6 -1.711219648331685 2216 7 -9.447729068301435 2216 8 -10.28650152723139 2216 9 -5.634945679082161 2216 10 -3.3147669191332625 2216 11 -2.3345219282464527 2216 12 -2.0431404516395872 2216 13 -1.7426910834672356 2216 14 -1.915118746762083 2216 15 -2.8025272922836386 2216 16 -1.8972784236213656 2216 17 -2.1074662983190606 2216 18 -1.8640851125386888 2216 19 -1.1727177235663646 2216 20 -2.2028356903459496 2216 21 -4.930264414881936 2216 22 -3.296050463061288 2216 23 -3.163457762867825 2216 24 -3.425098149379643 2216 25 -2.9205835309534436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 217852.0 3 13.0 4 1865.0 5 10583.0 6 12614.0 7 10365.0 8 8385.0 9 7661.0 10 7227.0 11 7012.0 12 6877.0 13 6631.0 14 6642.0 15 6928.0 16 7287.0 17 8059.0 18 9311.0 19 11435.0 20 14689.0 21 18783.0 22 23589.0 23 28193.0 24 33052.0 25 37454.0 26 42816.0 27 46665.0 28 52191.0 29 61668.0 30 73077.0 31 89368.0 32 114842.0 33 149935.0 34 204017.0 35 283126.0 36 410117.0 37 650689.0 38 800687.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.36581001933127 17.38706059932692 12.42555043457937 25.821578946762447 2 16.433546724644682 19.338400658004478 38.3971781731173 25.830874444233547 3 18.926362265305677 23.23984290248473 29.223980148837704 28.609814683371894 4 13.005317062052319 15.843746955098863 36.11440291770152 35.0365330651473 5 14.948250288934808 36.46752309539316 34.086033768959595 14.498192846712435 6 33.74992494858439 34.554389940438625 17.27147813511668 14.424206975860304 7 29.244242310572243 30.603460073230316 21.76096747678673 18.391330139410716 8 26.79446582016508 33.592521148135475 20.750956145778957 18.862056885920488 9 26.884614754830665 15.127219054748823 19.06201675762121 38.926149432799306 10 16.04740659525049 27.753355606353676 32.79707991279274 23.4021578856031 11 35.1783743970713 22.267434194556774 22.83339935589936 19.72079205247257 12 23.807957530726593 24.22500901294273 29.622002209068672 22.345031247262003 13 28.860962685128715 21.151748598670732 25.033903938314793 24.953384777885756 14 22.62992883279931 20.40201108960449 26.19983694799744 30.768223129598766 15 24.58937656017948 28.11827632663731 22.740141963765055 24.552205149418153 16 24.23451557886155 27.065544661911943 24.86705806411951 23.832881695106995 17 23.321751595421503 27.113504383010323 25.901256946529138 23.663487075039033 18 23.35384453636073 26.84305669301879 26.44643910252451 23.35665966809597 19 24.735439551284337 26.188353019993865 26.16255485700693 22.91365257171487 20 24.730320994931752 25.898003581069457 26.131350502128214 23.240324921870577 21 24.684336815024174 25.55114483291934 25.994358781820903 23.77015957023558 22 24.757763211492463 25.510629117594508 25.890681618271017 23.84092605264201 23 24.322247450779415 25.62541223028361 25.838077093670208 24.21426322526676 24 24.540351723860574 25.78778790855869 25.719534405395933 23.9523259621848 25 24.189298309668267 25.608685173380874 26.014010883182024 24.18800563376883 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1024.0 1 1024.0 2 1300.5 3 1577.0 4 1577.0 5 1577.0 6 2456.5 7 3336.0 8 3336.0 9 3336.0 10 4362.0 11 5388.0 12 5388.0 13 5388.0 14 8376.5 15 11365.0 16 11365.0 17 11365.0 18 19618.0 19 27871.0 20 27871.0 21 27871.0 22 42977.5 23 58084.0 24 58084.0 25 58084.0 26 82095.0 27 106106.0 28 106106.0 29 106106.0 30 137670.0 31 169234.0 32 169234.0 33 169234.0 34 205801.0 35 242368.0 36 242368.0 37 242368.0 38 283605.0 39 324842.0 40 324842.0 41 324842.0 42 362687.5 43 400533.0 44 400533.0 45 400533.0 46 431256.0 47 461979.0 48 461979.0 49 461979.0 50 469121.0 51 476263.0 52 476263.0 53 476263.0 54 449225.0 55 422187.0 56 422187.0 57 422187.0 58 373958.5 59 325730.0 60 325730.0 61 325730.0 62 272810.0 63 219890.0 64 219890.0 65 219890.0 66 172840.0 67 125790.0 68 125790.0 69 125790.0 70 93154.0 71 60518.0 72 60518.0 73 60518.0 74 42563.0 75 24608.0 76 24608.0 77 24608.0 78 16832.5 79 9057.0 80 9057.0 81 9057.0 82 5966.5 83 2876.0 84 2876.0 85 2876.0 86 1847.5 87 819.0 88 819.0 89 819.0 90 510.5 91 202.0 92 202.0 93 202.0 94 120.0 95 38.0 96 38.0 97 38.0 98 29.0 99 20.0 100 20.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21658928599637248 2 0.1299650602219315 3 0.13295210249001568 4 0.0726081043626614 5 0.02343679318035273 6 0.021770942684690403 7 0.3084121141796907 8 0.30091578694921023 9 0.019013672898766553 10 0.036217887500520574 11 0.016428732474462943 12 0.017232936162024065 13 0.0156819719074419 14 0.017433987083914346 15 0.015366034744471459 16 0.019358331622007032 17 0.01866901417552607 18 0.014877768219880776 19 0.024039945946023572 20 0.017864810487964948 21 0.015567085666361738 22 0.017433987083914346 23 0.01841052013309571 24 0.016227681552572662 25 0.01591174438960222 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3481705.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.08331006103765 #Duplication Level Percentage of deduplicated Percentage of total 1 87.64211961623475 66.68102561168537 2 7.841234995689829 11.931742268770568 3 2.095448467887913 4.782859664977271 4 0.8524355487928268 2.594244726634217 5 0.4308121552837478 1.6388807394258642 6 0.28031674125341427 1.279645532404991 7 0.17092153256652065 0.9102993170856434 8 0.11678196239765654 0.7108126603709874 9 0.08900516004473406 0.6094626469844202 >10 0.45293209131137796 5.965039685146233 >50 0.020112287444312104 1.043985447898 >100 0.007051121287032848 0.9171091764816354 >500 3.3885810627313443E-4 0.1825326395040626 >1k 4.5181079969252105E-4 0.590566986761982 >5k 3.765089997437671E-5 0.1617928958688238 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5648 0.16221937240518655 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.744312054008022E-5 2 0.0 0.0 0.0 0.0 5.744312054008022E-5 3 0.0 0.0 0.0 0.0 5.744312054008022E-5 4 0.0 0.0 0.0 0.0 5.744312054008022E-5 5 0.0 2.872156027004011E-5 0.0 0.0 5.744312054008022E-5 6 5.744312054008022E-5 2.872156027004011E-5 0.0 0.0 5.744312054008022E-5 7 5.744312054008022E-5 2.872156027004011E-5 0.0 0.0 5.744312054008022E-5 8 5.744312054008022E-5 2.872156027004011E-5 0.0 0.0 5.744312054008022E-5 9 5.744312054008022E-5 2.872156027004011E-5 0.0 2.872156027004011E-5 8.616468081012033E-5 10 5.744312054008022E-5 2.872156027004011E-5 0.0 2.872156027004011E-5 8.616468081012033E-5 11 5.744312054008022E-5 2.872156027004011E-5 0.0 2.872156027004011E-5 8.616468081012033E-5 12 5.744312054008022E-5 2.872156027004011E-5 0.0 2.872156027004011E-5 8.616468081012033E-5 13 5.744312054008022E-5 2.872156027004011E-5 0.0 2.872156027004011E-5 8.616468081012033E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1805 0.0 10.676268 1 GTATCAA 3690 0.0 10.625779 1 ATACCGA 85 6.4831093E-4 10.077947 6 GTATTCG 105 3.923101E-5 9.994216 1 CCGATCA 135 6.6064604E-7 9.834562 9 TAGGACA 460 0.0 9.5238285 4 TGGACCG 220 1.6370905E-11 9.518061 5 TAGGACC 305 0.0 9.3677 4 GTATTAT 320 0.0 9.24181 1 GTATTAA 300 0.0 9.221935 1 GGACCGT 145 1.6643644E-6 9.189852 6 TCTATAC 315 0.0 8.778101 3 GTCTTAC 250 2.3646862E-11 8.776739 1 GTCCTAA 360 0.0 8.744938 1 GTGTAAA 635 0.0 8.563404 1 CTAGGAC 380 0.0 8.531167 3 GTCTTAT 280 3.6379788E-12 8.517797 1 CTAGTAC 170 1.5305741E-6 8.413089 3 GTCTATG 445 0.0 8.360841 1 GTATTAG 275 2.0008883E-11 8.32576 1 >>END_MODULE