FastQCFastQC Report
Thu 26 May 2016
SRR1926719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481705
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT98770.2836828507871862No Hit
GGTATCAACGCAGAGTACTTTTTTT61670.17712586218533735No Hit
TATCAACGCAGAGTACTTTTTTTTT57620.1654936302759711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22750.012.31907751
GTATCAA47350.010.26744651
TGTGCGA1502.4978362E-810.12022710
ATACCGT1356.2422805E-79.875426
GTCTAGA3000.09.8503731
GTAGGAC4900.09.7180483
TAGGACG3100.09.5227264
ACAACGC1106.939854E-59.4875778
ACTAGAC2054.0381565E-109.2914023
ACACGAC1054.3844944E-49.0701773
TATACCG1054.38892E-49.0692625
TTACGGT850.0072942468.9625654
GTCCTAC5150.08.8847711
GTATAGG3800.08.7800441
CCCTAGG3050.08.7512571
ATTAGAC1651.0139538E-68.6578973
TTAGGAC3650.08.6104563
TTAGACT2553.8198777E-118.5891264
TAGACAG5250.08.5251065
TAGTACT3250.08.4972014
Produced by FastQC (version 0.11.5)