FastQCFastQC Report
Thu 26 May 2016
SRR1926718_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926718_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4112939
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64520.15687079239444104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTT707.404342E-613.547297515
ACGATAC909.2034265E-510.5911393
GGTATCA24400.010.4754911
GTATCAA45950.09.6100331
GTCTTAT4000.09.5373891
TCTTATA5000.09.1508562
CTTATAC3700.09.016783
GTATAGG5100.08.9763651
AGCGTCG2252.4556357E-108.8965573
GTATTAG3550.08.8657411
TATACTG4600.08.6900885
GTACTAG2659.094947E-128.6376341
TAGGACG3200.08.629014
CCGTCTA1107.269254E-48.6218479
TAGCGTC2252.3010216E-98.4730152
GTCCTAG5150.08.333641
CGCACTG1606.296157E-68.3280015
GTTCTAG4300.08.206591
GCATTAG3150.08.1749031
TTAACGT1050.0044234238.1614284
Produced by FastQC (version 0.11.5)