##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926718_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4112939 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.758815289990928 31.0 28.0 31.0 16.0 34.0 2 27.891564888270892 31.0 28.0 31.0 16.0 34.0 3 27.359548731454563 31.0 27.0 31.0 10.0 34.0 4 31.163993922594038 35.0 32.0 37.0 17.0 37.0 5 31.03713402994793 35.0 32.0 37.0 16.0 37.0 6 30.793019298365476 35.0 31.0 37.0 11.0 37.0 7 30.883513954376664 35.0 32.0 37.0 11.0 37.0 8 30.482399568775516 35.0 30.0 37.0 10.0 37.0 9 32.69665997963986 37.0 32.0 39.0 17.0 39.0 10 32.89132199626593 37.0 33.0 39.0 17.0 39.0 11 33.025536483765016 37.0 33.0 39.0 17.0 39.0 12 32.91030525859975 37.0 33.0 39.0 13.0 39.0 13 33.00210579344843 38.0 33.0 39.0 15.0 39.0 14 34.0065751035938 39.0 33.0 40.0 16.0 41.0 15 33.53454427600312 39.0 33.0 40.0 10.0 41.0 16 33.618441946257896 39.0 33.0 40.0 10.0 41.0 17 33.83659154682333 39.0 33.0 40.0 10.0 41.0 18 33.88492389505412 39.0 33.0 40.0 10.0 41.0 19 33.22482657778294 39.0 32.0 40.0 8.0 41.0 20 33.50778457934825 39.0 33.0 40.0 7.0 41.0 21 33.49562587726198 39.0 33.0 40.0 2.0 41.0 22 33.519951061759 39.0 33.0 40.0 2.0 41.0 23 33.51290865242592 39.0 34.0 40.0 2.0 41.0 24 32.15720145618498 38.0 31.0 40.0 2.0 41.0 25 32.732047083606155 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.34104000077994456 1101 2 -0.35740311436254757 1101 3 -1.4023078353754634 1101 4 -0.6901213948783571 1101 5 -0.48587041645507867 1101 6 -2.260778370343516 1101 7 -0.5619155707397283 1101 8 -0.21315354965577882 1101 9 -0.20371534645337164 1101 10 -9.111701717102072 1101 11 -3.9982528869296807 1101 12 -1.5911719396490547 1101 13 -0.8373167851778263 1101 14 -0.5710466481585286 1101 15 -2.163791326809605 1101 16 -0.537911008302018 1101 17 -0.391795732059812 1101 18 -0.29658564899335005 1101 19 -0.8793649734689382 1101 20 -0.12740023825525526 1101 21 -0.23296309132319948 1101 22 -0.031109536052511544 1101 23 -0.17075376386459595 1101 24 -0.3781100120644183 1101 25 -0.19722260226635768 1102 1 -0.18814051634509354 1102 2 -0.3394742122207042 1102 3 -0.6157102178554261 1102 4 -0.44831679355651843 1102 5 -0.28128300052464184 1102 6 -1.0602700297676328 1102 7 -0.012836754623052116 1102 8 0.4242092776247155 1102 9 0.3103890181085305 1102 10 0.36298909013644476 1102 11 0.10925014423614243 1102 12 0.16479260193543155 1102 13 -0.009836249521811169 1102 14 -0.20506944772360214 1102 15 0.32000140613093464 1102 16 0.16314482083137705 1102 17 0.06391462858493924 1102 18 -0.21733041238160666 1102 19 0.28154894243355244 1102 20 0.17430471612806286 1102 21 0.05719348808748492 1102 22 0.05486295735756386 1102 23 -0.3284868696437968 1102 24 -3.7443314846138414 1102 25 -2.000961448879636 1103 1 -0.08606590661267433 1103 2 -0.2366129182460348 1103 3 -0.03424893531452611 1103 4 -0.20533737953799047 1103 5 -0.4282954463411386 1103 6 0.4700136647702031 1103 7 0.2587911176098814 1103 8 0.31209977173830694 1103 9 0.09333827401278683 1103 10 0.18357496928086192 1103 11 -0.0849217518449521 1103 12 -0.17170122874370009 1103 13 -0.09698981756193348 1103 14 -0.02879955950229629 1103 15 0.29872188551122747 1103 16 0.23709806748726692 1103 17 -0.07799134409449238 1103 18 -0.17258527542936974 1103 19 -2.0149000913523736 1103 20 -0.5213742643524242 1103 21 -0.4221971162572231 1103 22 -0.22626781493537607 1103 23 -0.21112825125456425 1103 24 1.2669515127225992 1103 25 0.5002395681172302 1104 1 -0.43321691348399227 1104 2 -0.3322753555083757 1104 3 -1.61928081920221 1104 4 -0.6399726090267741 1104 5 -0.5499817805857425 1104 6 -1.8281391854557931 1104 7 -0.3280388263947813 1104 8 0.25772171995016535 1104 9 0.2009058911559194 1104 10 0.3061107775805496 1104 11 0.12130447085495888 1104 12 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5 -0.5382542856138919 2212 6 0.2383598486459917 2212 7 0.2579853698216148 2212 8 0.26431090390113354 2212 9 0.14392199655318194 2212 10 0.3019019526221598 2212 11 0.10994993198264069 2212 12 -0.0983392134182921 2212 13 -0.01975869982790357 2212 14 -0.18705688317548663 2212 15 -1.0621755376981667 2212 16 -0.1675014687929135 2212 17 -0.1696197622348592 2212 18 -0.12931281183336552 2212 19 0.6624303998752268 2212 20 -0.6927839417492123 2212 21 -0.812477756587235 2212 22 -0.11739656775189644 2212 23 0.017159506047107698 2212 24 1.2219703478987682 2212 25 0.7628156453674251 2213 1 -0.8190200689490652 2213 2 -0.7867587477162594 2213 3 -0.09881987158294336 2213 4 -7.106880264055594 2213 5 -2.0158578130761633 2213 6 -3.8286515734122126 2213 7 -1.6699785930617814 2213 8 -3.4356422701937817 2213 9 -1.5699637807229294 2213 10 -0.9079165213620186 2213 11 -0.7915559220140622 2213 12 -0.7556786720476438 2213 13 -0.7601511342894867 2213 14 -0.9466237538164037 2213 15 -3.4391856960727125 2213 16 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0.07825052631059748 2215 3 0.573678648120012 2215 4 0.8196485689928217 2215 5 1.000123255493648 2215 6 1.2284787684477187 2215 7 0.7357223923931038 2215 8 -0.4665212153218299 2215 9 0.004175907473303653 2215 10 -0.1727281041065325 2215 11 -0.17827533819036745 2215 12 -0.4547785388891299 2215 13 0.015411958426227557 2215 14 0.10077679000516326 2215 15 0.1951731749995531 2215 16 0.33805646347828144 2215 17 -0.15918186590097605 2215 18 -0.8817217829048261 2215 19 0.3653969380784048 2215 20 -3.6990350779545302 2215 21 -2.1529503006012156 2215 22 -1.0908043998989143 2215 23 -0.9793819842433749 2215 24 -1.0273967868197467 2215 25 -0.6502368309602318 2216 1 -0.584712807327076 2216 2 -1.1642825006853812 2216 3 -0.2086232309314333 2216 4 -0.5587777869602419 2216 5 -0.45002001192244734 2216 6 -2.0446829612642645 2216 7 -9.730169295471011 2216 8 -11.290197920007149 2216 9 -6.203461575090081 2216 10 -3.7734372203779714 2216 11 -2.7400710221158597 2216 12 -2.30383425399539 2216 13 -2.101500981591826 2216 14 -2.2472016296223067 2216 15 -3.274961230514517 2216 16 -2.3147512004258743 2216 17 -2.6156812504911144 2216 18 -2.256198766575949 2216 19 -1.6702411188675228 2216 20 -2.6104246734251113 2216 21 -5.256268344418398 2216 22 -3.7595463380297076 2216 23 -3.719284995648973 2216 24 -3.9282412112063874 2216 25 -3.3433466200797852 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 267649.0 3 63.0 4 1993.0 5 11318.0 6 14215.0 7 12040.0 8 9966.0 9 9057.0 10 8654.0 11 8632.0 12 8280.0 13 8033.0 14 8502.0 15 8323.0 16 9224.0 17 9798.0 18 11296.0 19 14013.0 20 18147.0 21 23117.0 22 29081.0 23 34801.0 24 40316.0 25 46443.0 26 52688.0 27 57124.0 28 63991.0 29 75511.0 30 89336.0 31 108682.0 32 139904.0 33 182560.0 34 245226.0 35 339542.0 36 484981.0 37 753984.0 38 906449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94985858223575 17.71837397835168 12.704646416604454 25.627121022808126 2 15.774902920621923 19.396107963515536 39.32073283458151 25.50825628128103 3 18.717905708483958 24.15654990733083 28.8437862968437 28.28175808734151 4 13.301384441179861 16.236661536223153 35.99002069533 34.47193332726698 5 14.3789632140777 36.56882784314822 34.17135311348274 14.880855829291336 6 34.029221363501506 34.88475362713579 17.371487770120087 13.714537239242619 7 29.137741910782424 30.889103059029182 21.75788705115518 18.21526797903322 8 26.691663500606953 33.883455513162595 20.053350747881016 19.37153023834943 9 26.878228057235727 15.28792343344308 19.13993697271063 38.69391153661057 10 15.859086553195215 28.052759846867048 32.91115564787205 23.17699795206569 11 34.93985454787744 22.47095954775464 22.87229398963738 19.716891914730535 12 23.737146281072526 24.36801991326369 29.790182173485263 22.104651632178516 13 28.657595033054445 21.384765627849678 25.079724125587934 24.877915213507944 14 22.479501349878483 20.648179986391785 26.382330525929774 30.489988137799955 15 24.460511603906504 28.256153093070047 22.933199297136753 24.3501360058867 16 23.995960276866455 27.26358366630895 25.151726719004085 23.58872933782051 17 23.241125347108316 27.262900610842543 25.98059087264715 23.51538316940199 18 23.20222705429369 27.064579808371782 26.57140536599861 23.161787771335916 19 24.55702137625234 26.42635821607673 26.30639205854679 22.710228349124133 20 24.565558420449424 26.100150453537047 26.29136043604533 23.0429306899682 21 24.55811386044114 25.816699353040807 26.071592278315254 23.553594508202803 22 24.51495425486979 25.80928501029001 26.004579984490206 23.67118075034999 23 24.20710952601782 25.821281595914208 25.989366764902787 23.98224211316519 24 24.364283647310867 26.08682753405037 25.840517084155056 23.70837173448371 25 24.097683439745698 25.77669355183858 26.139871954991655 23.985751053424064 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1204.0 1 1204.0 2 1504.5 3 1805.0 4 1805.0 5 1805.0 6 2780.0 7 3755.0 8 3755.0 9 3755.0 10 5270.0 11 6785.0 12 6785.0 13 6785.0 14 10880.0 15 14975.0 16 14975.0 17 14975.0 18 25489.5 19 36004.0 20 36004.0 21 36004.0 22 54902.0 23 73800.0 24 73800.0 25 73800.0 26 103335.0 27 132870.0 28 132870.0 29 132870.0 30 171621.0 31 210372.0 32 210372.0 33 210372.0 34 252902.5 35 295433.0 36 295433.0 37 295433.0 38 342681.5 39 389930.0 40 389930.0 41 389930.0 42 433230.0 43 476530.0 44 476530.0 45 476530.0 46 511395.5 47 546261.0 48 546261.0 49 546261.0 50 552320.0 51 558379.0 52 558379.0 53 558379.0 54 523940.0 55 489501.0 56 489501.0 57 489501.0 58 431493.0 59 373485.0 60 373485.0 61 373485.0 62 312018.5 63 250552.0 64 250552.0 65 250552.0 66 196405.0 67 142258.0 68 142258.0 69 142258.0 70 104665.0 71 67072.0 72 67072.0 73 67072.0 74 47297.5 75 27523.0 76 27523.0 77 27523.0 78 18794.0 79 10065.0 80 10065.0 81 10065.0 82 6616.0 83 3167.0 84 3167.0 85 3167.0 86 2039.5 87 912.0 88 912.0 89 912.0 90 569.5 91 227.0 92 227.0 93 227.0 94 138.0 95 49.0 96 49.0 97 49.0 98 37.0 99 25.0 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2136428476084863 2 0.12686791610573364 3 0.1292506404787428 4 0.06888018519117352 5 0.021979416665309164 6 0.02137157881505172 7 0.30044209262524924 8 0.2954821357671485 9 0.018283762535743906 10 0.035959687221230364 11 0.015998292218775915 12 0.01714102737725991 13 0.015390454368518472 14 0.017432789545383482 15 0.015001438144353708 16 0.018867286871991053 17 0.018235135507723307 18 0.014612421920188945 19 0.023000584253741668 20 0.017165340891270208 21 0.015171632742425794 22 0.016970832779187827 23 0.017943373339599736 24 0.015779470592683236 25 0.01543908139653907 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4112939.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.41753368760035 #Duplication Level Percentage of deduplicated Percentage of total 1 88.61215008558737 68.60134114381658 2 7.31821798456406 11.331167747063816 3 1.863269068069516 4.327490875390064 4 0.7823333529575748 2.422652748301055 5 0.389540129469067 1.507861804792185 6 0.24543027214511884 1.1400363817050996 7 0.15637015172835445 0.847405404241554 8 0.11690746665540615 0.7240550190501536 9 0.08362010118907749 0.5826295800689361 >10 0.40484945218288065 5.436048417694447 >50 0.018912332469628597 0.9962414164877637 >100 0.007397238498789114 0.9742056248337781 >500 4.698583832714519E-4 0.24252609458613592 >1k 5.011822116362525E-4 0.7098755695072382 >5k 3.132388822724828E-5 0.1564621724612868 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6452 0.15687079239444104 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4313514010297747E-5 2 0.0 0.0 0.0 0.0 2.4313514010297747E-5 3 0.0 0.0 0.0 0.0 2.4313514010297747E-5 4 0.0 0.0 0.0 2.4313514010297747E-5 2.4313514010297747E-5 5 0.0 0.0 0.0 2.4313514010297747E-5 2.4313514010297747E-5 6 0.0 0.0 0.0 2.4313514010297747E-5 4.8627028020595495E-5 7 0.0 2.4313514010297747E-5 0.0 2.4313514010297747E-5 4.8627028020595495E-5 8 0.0 2.4313514010297747E-5 0.0 2.4313514010297747E-5 4.8627028020595495E-5 9 0.0 2.4313514010297747E-5 0.0 2.4313514010297747E-5 7.294054203089324E-5 10 0.0 4.8627028020595495E-5 0.0 2.4313514010297747E-5 7.294054203089324E-5 11 0.0 4.8627028020595495E-5 0.0 2.4313514010297747E-5 7.294054203089324E-5 12 0.0 4.8627028020595495E-5 0.0 2.4313514010297747E-5 1.4588108406178647E-4 13 0.0 4.8627028020595495E-5 0.0 2.4313514010297747E-5 1.4588108406178647E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATTT 70 7.404342E-6 13.5472975 15 ACGATAC 90 9.2034265E-5 10.591139 3 GGTATCA 2440 0.0 10.475491 1 GTATCAA 4595 0.0 9.610033 1 GTCTTAT 400 0.0 9.537389 1 TCTTATA 500 0.0 9.150856 2 CTTATAC 370 0.0 9.01678 3 GTATAGG 510 0.0 8.976365 1 AGCGTCG 225 2.4556357E-10 8.896557 3 GTATTAG 355 0.0 8.865741 1 TATACTG 460 0.0 8.690088 5 GTACTAG 265 9.094947E-12 8.637634 1 TAGGACG 320 0.0 8.62901 4 CCGTCTA 110 7.269254E-4 8.621847 9 TAGCGTC 225 2.3010216E-9 8.473015 2 GTCCTAG 515 0.0 8.33364 1 CGCACTG 160 6.296157E-6 8.328001 5 GTTCTAG 430 0.0 8.20659 1 GCATTAG 315 0.0 8.174903 1 TTAACGT 105 0.004423423 8.161428 4 >>END_MODULE