FastQCFastQC Report
Thu 26 May 2016
SRR1926718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4112939
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT116680.2836900814721541No Hit
GGTATCAACGCAGAGTACTTTTTTT72830.17707532253699848No Hit
TATCAACGCAGAGTACTTTTTTTTT68220.1658667925782512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCG550.003099134712.0756279
GGTATCA31400.011.3230841
TGGACCG2500.010.2854255
ATAGGAC2800.010.2046663
TAGGACC3450.09.9376094
GGCGTAG1502.5251575E-79.5320341
GTCTTAA3350.09.3897641
GTCCTAG4300.09.3103581
GTATCAA58800.09.2240261
TAGGACT4900.09.1348264
AAACCGT1151.0819066E-49.1094756
GGACCGT1901.0155418E-89.0223036
GTCCTAA4650.09.0195591
GTAGGAC4750.08.6220483
TAGACTG5050.08.4863235
GTGTTAA3650.08.3568521
GCCTTAG3350.08.2516111
TTAGAAC3700.08.237283
GTATAGA3850.08.1703141
GACCGCG1050.0045628258.1333577
Produced by FastQC (version 0.11.5)