FastQCFastQC Report
Thu 26 May 2016
SRR1926717_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926717_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701498
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9320.1328585398675406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG804.5094566E-818.2393671
TTAATAC607.129358E-616.3113023
GTCCTAC6200.015.8324121
TGTAGGA6500.015.6621152
GATATAC1055.999573E-815.1599921
CTGTAGG6550.014.9864051
TCCTACA7400.014.8578432
CCTACAC1208.54925E-1114.4989353
TAGGACC3350.013.6914584
TGTAGGT700.00128299213.57383252
GACAGCG450.00739465213.5408517
AGGACCT4550.013.2325235
GTAGGAC7100.012.86525253
CTTTAGG3000.012.8228271
CTAAGAT600.002135655112.6865683
TAATACT1101.09823304E-712.6059824
GGACCTG4500.012.4520036
TAGGCCC600.002468348712.4443664
GTAGAAC1751.8189894E-1212.4276583
GTCTTAT750.002563746412.3806611
Produced by FastQC (version 0.11.5)