##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926717_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 701498 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.698200137420205 27.0 10.0 31.0 2.0 31.0 2 16.795213956418976 22.0 2.0 31.0 2.0 31.0 3 20.456041214657777 25.0 15.0 31.0 2.0 31.0 4 22.518320793501907 28.0 7.0 35.0 2.0 37.0 5 23.06270438404671 30.0 7.0 35.0 2.0 37.0 6 24.034052841205533 31.0 8.0 35.0 2.0 37.0 7 23.642026064222563 30.0 8.0 35.0 2.0 37.0 8 23.901812977371282 31.0 12.0 35.0 2.0 37.0 9 26.727952182329815 32.0 17.0 37.0 2.0 39.0 10 27.50213685570023 32.0 25.0 37.0 2.0 39.0 11 24.518664344018088 30.0 8.0 37.0 2.0 39.0 12 25.731862385922696 31.0 17.0 37.0 2.0 39.0 13 22.319531915985504 28.0 2.0 37.0 2.0 39.0 14 25.73543759212428 31.0 17.0 38.0 2.0 40.0 15 26.872177825168425 31.0 17.0 38.0 2.0 40.0 16 27.235400528583117 32.0 20.0 39.0 2.0 40.0 17 26.541545378604074 31.0 17.0 38.0 2.0 40.0 18 26.61036097038053 31.0 17.0 38.0 2.0 40.0 19 26.99528152610556 31.0 19.0 39.0 2.0 40.0 20 27.282707862317498 31.0 23.0 39.0 2.0 40.0 21 25.061636668957004 30.0 10.0 38.0 2.0 40.0 22 26.03930731092605 31.0 11.0 38.0 2.0 40.0 23 26.045666559277432 31.0 8.0 38.0 2.0 40.0 24 25.0869268337187 31.0 2.0 39.0 2.0 40.0 25 24.878642562060048 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.744279424574849 1101 2 10.601012717568736 1101 3 7.269129214610828 1101 4 8.654449040672361 1101 5 8.165076164576696 1101 6 7.774033900865309 1101 7 7.212723771669797 1101 8 5.626877952365351 1101 9 5.7441219226965465 1101 10 5.1897512599337965 1101 11 8.049287885083487 1101 12 5.865846498237865 1101 13 10.290349463366098 1101 14 7.493134069441108 1101 15 6.55671861421223 1101 16 6.103024962765911 1101 17 2.435628999503688 1101 18 4.761812759944764 1101 19 5.477195170541428 1101 20 3.2902527287669763 1101 21 0.6338949947579238 1101 22 4.602215630926413 1101 23 5.775930747472412 1101 24 7.18648839155194 1101 25 7.747577738956831 1102 1 5.875917231212654 1102 2 11.08297519953122 1102 3 7.0709690414506525 1102 4 8.288864625087946 1102 5 8.188236337736871 1102 6 7.236930726262134 1102 7 6.870389716835742 1102 8 7.2221340851214855 1102 9 6.061203452278072 1102 10 5.466717349399882 1102 11 8.035164508460113 1102 12 5.365648085539451 1102 13 10.119263604780237 1102 14 7.564833203640237 1102 15 5.410326117819736 1102 16 5.905748627989571 1102 17 6.873688162562853 1102 18 6.770993856625861 1102 19 5.382209600555857 1102 20 5.630151718665964 1102 21 4.859526307889237 1102 22 5.824780565991347 1102 23 6.2510209350625985 1102 24 7.761903254466802 1102 25 7.455333871712966 1103 1 4.55456962950937 1103 2 8.937999570091158 1103 3 5.8947298064419975 1103 4 7.309372530918829 1103 5 6.683670176179703 1103 6 6.341540292901545 1103 7 6.256294745984842 1103 8 5.740712654957207 1103 9 4.863437553009721 1103 10 4.148816270511478 1103 11 4.952138019413589 1103 12 3.669635645403954 1103 13 7.825135768185106 1103 14 5.858842904078802 1103 15 5.076425553951875 1103 16 4.8572112597976655 1103 17 2.367864979204917 1103 18 1.4250387589843392 1103 19 3.3809791262060074 1103 20 4.086653720343762 1103 21 6.214907077977692 1103 22 1.6356638262306973 1103 23 3.93208981507874 1103 24 5.54026455996425 1103 25 1.8481020806095465 1104 1 5.841627967421783 1104 2 10.479872514434966 1104 3 7.024120793235845 1104 4 7.304586844990233 1104 5 7.610888444472579 1104 6 7.398305324332874 1104 7 7.3034423956681636 1104 8 7.271735809763403 1104 9 5.907256715734874 1104 10 5.110386180568714 1104 11 8.055305340297085 1104 12 4.8744618200364656 1104 13 9.319390447391005 1104 14 7.140048783879937 1104 15 6.6140885932863895 1104 16 6.086468029167175 1104 17 6.434911906664407 1104 18 3.894362650211697 1104 19 4.627391669290983 1104 20 3.9505676371526235 1104 21 3.3226767025106945 1104 22 5.136988253801928 1104 23 5.693735575727398 1104 24 6.816902314891909 1104 25 5.814277580737063 1105 1 3.8560186858401764 1105 2 9.933368092578139 1105 3 6.725820706373408 1105 4 6.059730015621586 1105 5 2.295035541692471 1105 6 5.141452843165769 1105 7 6.088951861641963 1105 8 3.4573864906937963 1105 9 4.139183233160701 1105 10 3.827605769826217 1105 11 7.269621895464891 1105 12 4.767490220497699 1105 13 5.50239715764128 1105 14 3.9399857959136853 1105 15 4.812928649569184 1105 16 4.956677260967968 1105 17 5.477263694455917 1105 18 2.8730216668882704 1105 19 2.8566843753860844 1105 20 1.9710095180545686 1105 21 2.982183567691049 1105 22 4.128699494614068 1105 23 1.855092712416365 1105 24 4.7908816812532855 1105 25 5.41899126487273 1106 1 6.585747833190567 1106 2 11.24150684715094 1106 3 7.809132183842166 1106 4 9.15860225300952 1106 5 8.64639042059867 1106 6 7.603016073105469 1106 7 7.1280276848692665 1106 8 7.143992478092503 1106 9 6.081923330607069 1106 10 5.302903717591317 1106 11 7.4901407967859726 1106 12 4.963931962613273 1106 13 4.396793313964274 1106 14 5.427977999218783 1106 15 5.224076864345228 1106 16 5.321737306108027 1106 17 5.613508844608784 1106 18 5.818734161916829 1106 19 5.908731500324059 1106 20 5.053603753770378 1106 21 5.3407625794119475 1106 22 5.683425963851477 1106 23 6.0059954519720335 1106 24 6.944311697543601 1106 25 6.055478368681314 1107 1 3.5905947950281494 1107 2 -14.10533648878047 1107 3 -5.89639549056905 1107 4 1.383468297277826 1107 5 4.1340491456186435 1107 6 4.539242638487838 1107 7 4.509860160185493 1107 8 5.036593332770387 1107 9 3.2457365915525926 1107 10 2.641734142951158 1107 11 5.375709739091548 1107 12 2.7344064823150873 1107 13 4.47755402126031 1107 14 3.6383049904740936 1107 15 3.3926369104753533 1107 16 2.970263854142729 1107 17 3.664384535155776 1107 18 -1.6080086064283243 1107 19 0.9961258567134941 1107 20 1.4662466582091795 1107 21 -2.237456450731454 1107 22 1.0973119139537282 1107 23 -1.855534147785587 1107 24 1.7051130619007502 1107 25 2.4153092411710944 1108 1 4.927040288483255 1108 2 10.305280216918202 1108 3 0.3019941638692174 1108 4 5.7893436542555 1108 5 6.901493026403394 1108 6 6.938384668494766 1108 7 7.01566418280693 1108 8 6.627645581821504 1108 9 5.036094340078797 1108 10 4.001877203207279 1108 11 6.399248853077243 1108 12 1.8796508177798863 1108 13 2.8837010116356794 1108 14 4.322564202149927 1108 15 4.214616090142492 1108 16 4.270107970832523 1108 17 -0.4301139096818467 1108 18 2.916197355312967 1108 19 4.13958132718988 1108 20 4.32493917165014 1108 21 4.575555156090218 1108 22 1.7227074342051019 1108 23 4.336248207789872 1108 24 5.135408506045824 1108 25 5.848306336406743 1109 1 3.8214051545043155 1109 2 -14.10533648878047 1109 3 -5.8111920217851765 1109 4 2.1840278730549336 1109 5 1.139747443453583 1109 6 2.8521070219290827 1109 7 3.9787538993821663 1109 8 4.083380899055214 1109 9 3.4859440154532173 1109 10 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4.751446368539607 2204 9 3.1357161759696304 2204 10 2.704060146505455 2204 11 4.385668374018721 2204 12 4.412347483060017 2204 13 2.1771818118772757 2204 14 3.8392700907596087 2204 15 3.9953815733131464 2204 16 3.8428812945930417 2204 17 -1.3313562860947386 2204 18 2.363145166386662 2204 19 3.0165706774351833 2204 20 3.470892283859083 2204 21 4.885963121278607 2204 22 4.384147040960013 2204 23 2.997353525829496 2204 24 4.7057677334590196 2204 25 4.938135032433834 2205 1 5.77849776418887 2205 2 10.141665909301064 2205 3 7.043903680752198 2205 4 7.720293507955677 2205 5 7.903451178850993 2205 6 7.373371573584276 2205 7 7.314276814949459 2205 8 7.109583216725291 2205 9 5.867446120481173 2205 10 5.0048705930387385 2205 11 7.331130982034498 2205 12 6.4192266512583025 2205 13 9.59955923840321 2205 14 7.136562184092238 2205 15 6.098650782763102 2205 16 5.566185862617456 2205 17 6.385617580067805 2205 18 6.249274219348674 2205 19 4.684509319222485 2205 20 5.225698657738082 2205 21 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0.17898150755613074 2207 10 -0.25980612712359274 2207 11 -1.9874795141839101 2207 12 -3.1453409254495597 2207 13 0.12616295542958866 2207 14 -6.124997798690764 2207 15 -2.199470335726719 2207 16 -1.1080708858299388 2207 17 -0.013424724550038292 2207 18 -0.020704028822024156 2207 19 -0.5491402995440424 2207 20 -0.9482698597556123 2207 21 -4.749010988148058 2207 22 -5.098150669439889 2207 23 -2.174740803199139 2207 24 -0.3394120968485481 2207 25 0.18826760464670045 2208 1 4.78976648448425 2208 2 6.678137114892063 2208 3 6.2167264019172706 2208 4 8.184802084720893 2208 5 3.734043629144775 2208 6 6.133019096575829 2208 7 6.544440656332359 2208 8 6.649183365221646 2208 9 4.83975289633058 2208 10 4.3835307865365785 2208 11 6.473454460665518 2208 12 5.9743143332167925 2208 13 8.748939351757056 2208 14 3.2110085183790567 2208 15 4.4355772630020205 2208 16 5.0827519517507405 2208 17 5.845007680841054 2208 18 0.7175832752409121 2208 19 3.1423783721056573 2208 20 4.107951080742296 2208 21 6.831786689053597 2208 22 5.6430015091644705 2208 23 1.4882866819400533 2208 24 2.7679229138433854 2208 25 4.165385392495168 2209 1 2.494520004057602 2209 2 4.352653626540782 2209 3 4.7710669254447495 2209 4 0.05094233321178265 2209 5 4.653500891963532 2209 6 4.941111121649286 2209 7 5.559783210492004 2209 8 3.9127792101776464 2209 9 3.7484065909845086 2209 10 3.084850760469866 2209 11 -0.8429926515133594 2209 12 2.664291884080285 2209 13 1.6971576012286 2209 14 3.361495252464561 2209 15 3.6667340426790602 2209 16 3.235466583016425 2209 17 0.7787594092733059 2209 18 2.7304615923497764 2209 19 3.210867145646681 2209 20 3.53097865120624 2209 21 5.597797612367298 2209 22 4.563182917346747 2209 23 0.5738335219814452 2209 24 -3.0718102931058873 2209 25 0.13878612785879696 2210 1 -2.281472101132074 2210 2 -14.10533648878047 2210 3 -7.904648449889407 2210 4 -2.369177823097232 2210 5 -0.8712710594154878 2210 6 -1.2728261872079827 2210 7 -1.109215256692071 2210 8 -21.13582046033306 2210 9 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-4.970743347604969 2211 22 -6.600393436652716 2211 23 -8.579919456880788 2211 24 -22.4531941481306 2211 25 -22.250834959455865 2212 1 -0.9289574917655159 2212 2 -14.10533648878047 2212 3 -7.943236580513588 2212 4 -19.831265245041923 2212 5 -20.246828597328065 2212 6 -21.200569273737866 2212 7 -9.8589405793049 2212 8 -9.513362458564714 2212 9 -5.023936264327158 2212 10 -5.513840167795564 2212 11 -5.858998081823167 2212 12 -2.7657542764663603 2212 13 -6.470239564464311 2212 14 -4.035033165622682 2212 15 -3.7070134391749967 2212 16 -3.8528211068732645 2212 17 -4.0442602683166164 2212 18 -3.2107736574692396 2212 19 -3.7945260201452804 2212 20 -3.8507897536548192 2212 21 -1.6455627122859902 2212 22 -2.982987097379766 2212 23 -3.069082094092156 2212 24 -2.2241932639573 2212 25 -2.613346011622088 2213 1 -19.012069781774358 2213 2 -14.10533648878047 2213 3 -8.586745891781684 2213 4 -5.610192808541452 2213 5 -20.246828597328065 2213 6 -10.689750835355929 2213 7 -20.90314924420322 2213 8 -10.564795060521208 2213 9 -8.201014633023266 2213 10 -6.883969416797232 2213 11 -6.885761167463212 2213 12 -4.935462316288259 2213 13 -19.46758704457041 2213 14 -12.401615740172298 2213 15 -6.776974587119728 2213 16 -6.0847440049654296 2213 17 -4.661074699222588 2213 18 -4.645481659861414 2213 19 -5.432708665561844 2213 20 -5.395245038856853 2213 21 -3.5711640624177825 2213 22 -4.573251376196286 2213 23 -4.340273765063241 2213 24 -3.1907294256470635 2213 25 -4.227316615147306 2214 1 -19.012069781774358 2214 2 -14.10533648878047 2214 3 -12.758796531958987 2214 4 -19.831265245041923 2214 5 -20.246828597328065 2214 6 -21.200569273737866 2214 7 -16.77814924420322 2214 8 -15.362515375587298 2214 9 -14.588972642813275 2214 10 -14.681986700787219 2214 11 -15.026620105231281 2214 12 -13.106960873298322 2214 13 -19.46758704457041 2214 14 -17.326730740889808 2214 15 -24.216297258803642 2214 16 -18.89357175501725 2214 17 -23.946117032242345 2214 18 -18.438009004919373 2214 19 -15.79909955638889 2214 20 -14.441180550759526 2214 21 -11.94642515590121 2214 22 -13.065338848492475 2214 23 -13.029369225624173 2214 24 -11.819719571859412 2214 25 -11.558038349286374 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 116263.0 3 3.0 4 565.0 5 2134.0 6 3064.0 7 2893.0 8 2441.0 9 2389.0 10 2147.0 11 2084.0 12 1951.0 13 2183.0 14 3300.0 15 7099.0 16 10532.0 17 10995.0 18 11239.0 19 13279.0 20 15632.0 21 18511.0 22 19248.0 23 20809.0 24 21928.0 25 22549.0 26 18920.0 27 15016.0 28 16088.0 29 18360.0 30 17876.0 31 21009.0 32 26674.0 33 34266.0 34 41638.0 35 47199.0 36 47196.0 37 48478.0 38 35540.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60371067631754 18.993418227476898 12.916177510290652 25.48669358591491 2 13.189432474899466 19.641890152423056 42.991158219925964 24.17751915275152 3 18.989177293418948 24.11172409634922 29.327876759820327 27.571221850411504 4 11.034335539171416 17.563342583622063 38.914760505098606 32.48756137210791 5 12.00592035063162 39.111029521438326 36.02710379790821 12.855946330021851 6 31.502803422148446 37.99238159044664 18.21005517293989 12.294759814465028 7 27.492746432877812 32.068145850080384 21.92649718649518 18.512610530546624 8 23.662011233295317 39.321759821447785 19.966613687158567 17.04961525809833 9 26.051757305217972 15.931054953962429 20.31375761346706 37.70343012735254 10 13.936081481727758 30.012272563664826 35.10097761305141 20.950668341556 11 32.969972197596086 22.415612768600774 25.067696742716194 19.546718291086947 12 23.58360292942663 24.744549535832412 32.29650645673849 19.375341078002467 13 28.227470415697383 21.738646707798118 25.369171639526648 24.664711236977848 14 19.537499457158987 23.230410677320826 27.986276979198333 29.245812886321854 15 21.7159270277724 32.84527728390281 23.45646956654403 21.982326121780766 16 20.922487841733478 28.686173685330125 29.22311794435438 21.16822052858201 17 20.644529302385603 29.13022565564491 29.43700471020231 20.788240331767177 18 20.29629501054477 27.897931257937508 31.859524574086773 19.946249157430955 19 22.523817096265883 27.177077761554365 27.594292570800743 22.70481257137901 20 22.68087369507596 28.06426616876303 29.073607580640086 20.181252555520928 21 21.29585651687752 26.74734564859082 26.945370462737834 25.011427371793822 22 21.826873242862145 29.293207133074873 28.220032022977787 20.65988760108519 23 21.148137576709004 28.69373483659198 28.77465391751106 21.383473669187953 24 22.03318029691254 27.615648794701407 29.37079990058104 20.98037100780501 25 21.69596844285048 28.01167979882888 29.163390167219212 21.12896159110143 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 709.0 1 709.0 2 1454.5 3 2200.0 4 2200.0 5 2200.0 6 3253.0 7 4306.0 8 4306.0 9 4306.0 10 4959.0 11 5612.0 12 5612.0 13 5612.0 14 7112.0 15 8612.0 16 8612.0 17 8612.0 18 12357.5 19 16103.0 20 16103.0 21 16103.0 22 22670.0 23 29237.0 24 29237.0 25 29237.0 26 38255.0 27 47273.0 28 47273.0 29 47273.0 30 57324.0 31 67375.0 32 67375.0 33 67375.0 34 73930.0 35 80485.0 36 80485.0 37 80485.0 38 83654.0 39 86823.0 40 86823.0 41 86823.0 42 87279.0 43 87735.0 44 87735.0 45 87735.0 46 85606.5 47 83478.0 48 83478.0 49 83478.0 50 76189.5 51 68901.0 52 68901.0 53 68901.0 54 58669.0 55 48437.0 56 48437.0 57 48437.0 58 39816.0 59 31195.0 60 31195.0 61 31195.0 62 24670.0 63 18145.0 64 18145.0 65 18145.0 66 13525.0 67 8905.0 68 8905.0 69 8905.0 70 6417.0 71 3929.0 72 3929.0 73 3929.0 74 2667.5 75 1406.0 76 1406.0 77 1406.0 78 938.5 79 471.0 80 471.0 81 471.0 82 303.0 83 135.0 84 135.0 85 135.0 86 78.0 87 21.0 88 21.0 89 21.0 90 12.5 91 4.0 92 4.0 93 4.0 94 2.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.694120296850453 2 35.76774274481181 3 5.4280981556611705 4 13.187207946423227 5 13.029117688147366 6 14.039241736968602 7 9.204587896187872 8 7.260462610014569 9 0.10919489435465247 10 1.3843232625039559 11 5.452046905336864 12 5.4397874263362125 13 15.435254270147599 14 1.5235966460346286 15 2.824526940917864 16 2.8315119929066084 17 2.7903144413811587 18 1.4483291470538762 19 1.3924487311439235 20 0.28966582941077523 21 4.25731791109882 22 1.5315795625931934 23 0.11375656095954657 24 8.807010141155072 25 0.1141842172037554 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 701498.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 92.95398897591036 #Duplication Level Percentage of deduplicated Percentage of total 1 97.28668589336047 90.43185528034283 2 1.955960318471666 3.6362862776106675 3 0.3352470800486219 0.9348766014913721 4 0.14440417602240774 0.5369177674424923 5 0.07316384577565119 0.3400435656832543 6 0.040340505358561034 0.22498865342294008 7 0.023216413215492643 0.15106407526651577 8 0.020622358008878024 0.15335443512116198 9 0.01831640674402751 0.1532324763506361 >10 0.08898653839198284 1.5860791575266406 >50 0.006449730567326913 0.42902375109336616 >100 0.005820218082499392 0.9170892740052009 >500 7.86515952441602E-4 0.5051886846429031 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 932 0.1328585398675406 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 80 4.5094566E-8 18.239367 1 TTAATAC 60 7.129358E-6 16.311302 3 GTCCTAC 620 0.0 15.832412 1 TGTAGGA 650 0.0 15.662115 2 GATATAC 105 5.999573E-8 15.159992 1 CTGTAGG 655 0.0 14.986405 1 TCCTACA 740 0.0 14.857843 2 CCTACAC 120 8.54925E-11 14.498935 3 TAGGACC 335 0.0 13.691458 4 TGTAGGT 70 0.001282992 13.5738325 2 GACAGCG 45 0.007394652 13.540851 7 AGGACCT 455 0.0 13.232523 5 GTAGGAC 710 0.0 12.8652525 3 CTTTAGG 300 0.0 12.822827 1 CTAAGAT 60 0.0021356551 12.686568 3 TAATACT 110 1.09823304E-7 12.605982 4 GGACCTG 450 0.0 12.452003 6 TAGGCCC 60 0.0024683487 12.444366 4 GTAGAAC 175 1.8189894E-12 12.427658 3 GTCTTAT 75 0.0025637464 12.380661 1 >>END_MODULE