FastQCFastQC Report
Thu 26 May 2016
SRR1926717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926717_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences701498
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA18870.2689957776073489No Hit
GTCCTACAGTGGACATTTCTAAATT18710.2667149443049018No Hit
GTCCTACAGTGTGCATTTCTCATTT16160.23036416354715195No Hit
CTGTAGGACCTGGAATATGGCGAGA14870.21197494504617262No Hit
CTTTAGGACGTGAAATATGGCGAGG13810.1968644244174609No Hit
GTCCTAAAGTGTGTATTTCTCATTT12660.18047093505612274No Hit
GTATCAACGCAGAGTACTTTTTTTT11820.16849656021827575No Hit
GGTATCAACGCAGAGTACTTTTTTT7990.1138991130409495No Hit
CCCATGTACTCTGCGTTGATACCAC7570.10791192562202599No Hit
CTGAAGGACCTGGAATATGGCGAGA7500.10691406105220543No Hit
GTACATGGGAAGCAGTGGTATCAAC7120.10149708195889369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA456.8792497E-414.74251719
TAGGACC4950.014.2483694
TGTAGGA11450.013.7385732
GTAGGAC10500.013.7074743
ATAATTC500.001460976413.3443613
CTGTAGG10900.013.2861791
TCCAACG701.11381625E-412.185142518
AGGCGTG550.003056492712.0956857
GTCCTAC10350.012.0590741
GGCGAGG3550.012.0135219
TAGTACT802.7672275E-511.9137544
AGGACCT7300.011.8811155
CTGTCCA1459.640644E-1111.7977629
GTGTTAG657.8210974E-411.7262631
GTCCAAT658.132095E-411.67189811
TAGGACA2950.011.6310564
TGGCGAG9500.011.37279818
GACGTGG4350.011.3608077
GTCCTAA3800.011.2826711
GGACCAT1104.748308E-711.2639136
Produced by FastQC (version 0.11.5)