FastQCFastQC Report
Thu 26 May 2016
SRR1926716_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926716_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713453
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA12430.1742231093008229No Hit
GCTTACTCTGCGTTGATACCACTGC8020.11241104880069185No Hit
GTCCTACAGTGGACATTTCTAAATT7180.10063732299114307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAG308.16121E-526.4788131
GCCTAAC300.002533359222.0656781
ATTGGAC452.4269825E-416.9046673
GTTCTAG1053.4615368E-916.3916471
TAAGACG400.002153979616.300933
GTATAAT604.8233944E-415.4459731
GTTCTAA709.03433E-515.1307511
GTGTAGA1056.144546E-815.1307511
TTGGACT506.285712E-414.9177144
TCGTCAA550.004126235414.7848662
CGTCAAG450.00471991914.4897153
CAGTAGG550.004820293314.4429891
TGTAGAA2350.013.2644382
GTATAAG700.001548673613.2394071
CTTTAGA600.00857714713.2394071
CTAGAGT653.045322E-413.1144744
TATACTG1358.54925E-1113.048085
TAGGACC2000.012.78661254
GTAGGAC5200.012.5391773
GTCTTGT750.002601308512.3567791
Produced by FastQC (version 0.11.5)