##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926716_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 713453 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.78597468929278 27.0 10.0 31.0 2.0 31.0 2 16.86528615059436 22.0 2.0 31.0 2.0 31.0 3 20.52889258297323 25.0 15.0 31.0 2.0 31.0 4 22.58914322316957 28.0 7.0 35.0 2.0 37.0 5 23.14751216968742 30.0 7.0 35.0 2.0 37.0 6 24.150143036752247 31.0 10.0 35.0 2.0 37.0 7 23.754024441694128 31.0 10.0 35.0 2.0 37.0 8 24.01142331730331 31.0 12.0 35.0 2.0 37.0 9 26.829674834922553 32.0 17.0 37.0 2.0 39.0 10 27.61715067425605 32.0 25.0 38.0 2.0 39.0 11 24.64857951399742 30.0 10.0 37.0 2.0 39.0 12 25.870532466749736 31.0 17.0 37.0 2.0 39.0 13 22.463961886767592 29.0 2.0 37.0 2.0 39.0 14 25.843544003599398 31.0 17.0 38.0 2.0 40.0 15 26.973881951579152 31.0 18.0 38.0 2.0 40.0 16 27.326558301668086 32.0 20.0 39.0 2.0 40.0 17 26.650698784643136 31.0 17.0 38.0 2.0 40.0 18 26.719189631272137 31.0 17.0 38.0 2.0 40.0 19 27.136833120051357 31.0 20.0 39.0 2.0 40.0 20 27.41769534923814 32.0 24.0 39.0 2.0 40.0 21 25.17595693058968 30.0 10.0 38.0 2.0 40.0 22 26.14186218293286 31.0 16.0 38.0 2.0 40.0 23 26.151366663256024 31.0 10.0 38.0 2.0 40.0 24 25.204195651290274 31.0 2.0 39.0 2.0 40.0 25 24.98614624929743 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.6695566331839125 1101 2 10.42643959187976 1101 3 7.127358027819259 1101 4 8.495215077574379 1101 5 7.954309963444828 1101 6 7.821656431246787 1101 7 7.225347738139398 1101 8 5.257658385915992 1101 9 5.458938318054571 1101 10 5.148286818621251 1101 11 8.025250986658605 1101 12 5.666814295682396 1101 13 9.972090401316965 1101 14 7.366981528669982 1101 15 6.618694652105987 1101 16 6.019458718536594 1101 17 1.6884087826000496 1101 18 4.20596233291041 1101 19 4.940012275639475 1101 20 2.942505810316142 1101 21 0.6227839356180347 1101 22 4.1358164099190375 1101 23 5.460358396156327 1101 24 6.741619345227313 1101 25 7.323356989647227 1102 1 5.838334126890839 1102 2 10.673323843681473 1102 3 6.988685065617105 1102 4 8.296162486700442 1102 5 7.953389401477153 1102 6 6.763560658319562 1102 7 6.903811760484118 1102 8 6.8524688794603605 1102 9 5.82625301108564 1102 10 5.305184444741322 1102 11 7.980795540626321 1102 12 5.122309588809031 1102 13 9.854483222041985 1102 14 7.210017400414458 1102 15 5.177061359659156 1102 16 5.657204345405422 1102 17 6.632413368784356 1102 18 6.4704918179868365 1102 19 5.342423470658218 1102 20 5.628596642381005 1102 21 4.863194081380861 1102 22 5.97722544238686 1102 23 6.158084023123681 1102 24 7.526025579679359 1102 25 7.207096478615263 1103 1 4.809982842128388 1103 2 9.037669978264073 1103 3 6.154301544168984 1103 4 7.516674798058663 1103 5 6.666816421202647 1103 6 6.383107563911402 1103 7 6.155239063520092 1103 8 5.773764847851005 1103 9 4.821620125821326 1103 10 4.024167638358744 1103 11 4.630934919265723 1103 12 3.6054500855130165 1103 13 8.001322160032405 1103 14 6.077519790846658 1103 15 5.096540473099708 1103 16 4.7851949152136335 1103 17 2.308351075891906 1103 18 1.3339804324468005 1103 19 3.6619616492753586 1103 20 4.030989224705468 1103 21 6.267192300572113 1103 22 1.7694924574579645 1103 23 4.075382657668335 1103 24 5.533888710361381 1103 25 1.324306072722404 1104 1 5.376042620285965 1104 2 10.27011806410825 1104 3 6.910818203984846 1104 4 7.3571547045992745 1104 5 7.152075242514105 1104 6 6.836006072084462 1104 7 6.685358391131718 1104 8 6.552193160111678 1104 9 5.593269541854681 1104 10 4.294495827923061 1104 11 7.415876955283437 1104 12 4.509843356684321 1104 13 8.762715768077822 1104 14 6.399999184349621 1104 15 5.630524890353843 1104 16 5.260074305007894 1104 17 5.653207564427369 1104 18 3.380176072863044 1104 19 4.087081950422277 1104 20 3.2578458183209307 1104 21 2.6225660608599064 1104 22 4.63804541314202 1104 23 4.999715785992652 1104 24 5.910484921918325 1104 25 4.902152118160231 1105 1 3.083102097773459 1105 2 9.217920897514198 1105 3 6.04941472156969 1105 4 5.304753220291175 1105 5 2.150038074718182 1105 6 4.690297495247481 1105 7 5.491542006653855 1105 8 2.863936921660887 1105 9 3.494340707517285 1105 10 3.1651056261593205 1105 11 6.5605879809283145 1105 12 3.8154065073836563 1105 13 4.88097966844289 1105 14 3.2890997357069836 1105 15 3.7702000852819566 1105 16 4.095676666680294 1105 17 4.70660059058304 1105 18 1.8664131715515033 1105 19 1.7863473080533936 1105 20 1.1691058276354163 1105 21 1.9846140454759862 1105 22 3.4343947363395912 1105 23 0.9414251440444197 1105 24 3.8254046220520337 1105 25 4.802748545604906 1106 1 6.853661491577782 1106 2 11.427745676605745 1106 3 8.216789540114554 1106 4 9.370070443018694 1106 5 9.006514743051326 1106 6 8.03429607372344 1106 7 7.771003995944238 1106 8 7.676364414899613 1106 9 6.631595479561451 1106 10 6.0720060411720596 1106 11 8.46328706958684 1106 12 5.819428938551173 1106 13 4.800823782670779 1106 14 6.143668791056069 1106 15 6.3783690298003535 1106 16 6.3081776454839265 1106 17 6.933283078731989 1106 18 6.788597981723836 1106 19 6.678295217630005 1106 20 6.134035745295442 1106 21 6.4826630639618195 1106 22 6.916920832750247 1106 23 7.248269541372441 1106 24 8.084629770980772 1106 25 7.001620361481404 1107 1 3.9587920955654674 1107 2 -14.08941500982171 1107 3 -5.780654561668063 1107 4 1.4632799519245552 1107 5 4.451013172138413 1107 6 4.881892203225497 1107 7 4.7932463384972905 1107 8 5.35855896697845 1107 9 3.545045773369182 1107 10 3.109562072006799 1107 11 5.828288624826797 1107 12 2.756623767920857 1107 13 4.6417239410882445 1107 14 3.986434031804805 1107 15 3.6365460956774243 1107 16 3.4895909607491475 1107 17 4.153848655605447 1107 18 -0.9558290751111471 1107 19 1.7742487002824525 1107 20 1.9674080600952983 1107 21 -1.5296233316671106 1107 22 1.6584821489896022 1107 23 -1.0929718053447992 1107 24 2.5528642963432375 1107 25 3.4844126561716458 1108 1 5.5407068486807525 1108 2 10.972940648376905 1108 3 0.18370156532535376 1108 4 6.355919992474114 1108 5 7.565090342426341 1108 6 7.573519813573725 1108 7 7.640409118569465 1108 8 7.283364399286977 1108 9 5.738028605246754 1108 10 4.895277632543795 1108 11 7.434630680837067 1108 12 2.7717263376064913 1108 13 3.944403567432609 1108 14 5.466619122464245 1108 15 5.258878337932494 1108 16 5.190441573454439 1108 17 0.5311148441966012 1108 18 4.1350243099086015 1108 19 5.1170804826664735 1108 20 5.35231417620173 1108 21 5.045193463561176 1108 22 2.096537578621003 1108 23 4.843687353465985 1108 24 5.6226861662157965 1108 25 5.969574229751608 1109 1 3.482533512130246 1109 2 -14.08941500982171 1109 3 -5.812740397301848 1109 4 2.041809252869598 1109 5 1.6468026431245448 1109 6 2.563594459589222 1109 7 3.8630851916298745 1109 8 3.9084165825584556 1109 9 3.463245860004527 1109 10 2.4497416481087733 1109 11 1.0838154728125247 1109 12 1.4275350538405611 1109 13 2.380875939980953 1109 14 3.012318263403124 1109 15 3.174515701537871 1109 16 3.0538305561774663 1109 17 3.5049178844227207 1109 18 3.7616448637981215 1109 19 2.2114316674661083 1109 20 1.1726505237174258 1109 21 0.07859267229043354 1109 22 2.507571324441553 1109 23 2.9875618991363986 1109 24 4.224758560211942 1109 25 4.526238560139699 1110 1 3.035561306600787 1110 2 9.399492138904666 1110 3 6.456519983892701 1110 4 7.342074565582941 1110 5 8.283167518283125 1110 6 4.18745202740612 1110 7 6.407418441840068 1110 8 7.21002753628067 1110 9 5.44977514183422 1110 10 5.0910530314822395 1110 11 2.786193067174324 1110 12 5.790706869372599 1110 13 10.058827595017192 1110 14 7.695299549795045 1110 15 6.626433052607773 1110 16 6.460966337146626 1110 17 6.716988182071702 1110 18 6.819942801662894 1110 19 5.485495476059409 1110 20 5.717372817480054 1110 21 3.5622582937858063 1110 22 5.781634451697631 1110 23 6.432178721897422 1110 24 7.034081569923725 1110 25 7.507532446773361 1111 1 4.758426773098133 1111 2 9.386474318241532 1111 3 7.247604780629313 1111 4 7.83045102424985 1111 5 8.180752131519196 1111 6 7.597575411221047 1111 7 7.458468617058724 1111 8 7.490203956623574 1111 9 5.784752293581047 1111 10 5.118306596751594 1111 11 7.017614855346494 1111 12 6.27948085841453 1111 13 8.349827624247368 1111 14 5.9203387669460135 1111 15 5.472612831172963 1111 16 5.344493185062031 1111 17 6.674323819175832 1111 18 6.447880353018686 1111 19 5.320997147051056 1111 20 4.696451807155306 1111 21 7.676554772199321 1111 22 7.000460632840269 1111 23 7.148310855277959 1111 24 6.742388460775004 1111 25 7.194211606633186 1112 1 -2.4756126502616773 1112 2 7.991491139045603 1112 3 6.078161082354885 1112 4 -0.05780191459405515 1112 5 4.053957805822382 1112 6 3.2020204526015057 1112 7 6.167851811604436 1112 8 6.337573387530362 1112 9 5.035707178009968 1112 10 3.623416155829073 1112 11 -0.3497086541255996 1112 12 4.106808589077001 1112 13 8.343586658234347 1112 14 5.914248740467638 1112 15 4.056650335809877 1112 16 4.27834713187503 1112 17 5.254012218577191 1112 18 5.184449932306709 1112 19 4.8359587086356655 1112 20 4.518767833608102 1112 21 -1.596265238801358 1112 22 2.907839401531831 1112 23 4.1622427022406185 1112 24 5.961032691175248 1112 25 6.144438015935155 1113 1 -19.059755045083683 1113 2 -14.08941500982171 1113 3 -7.675892839762717 1113 4 -6.526264310105237 1113 5 -1.2842313322220313 1113 6 -0.4950115049683532 1113 7 -20.913863398751552 1113 8 -9.631293179780396 1113 9 -5.392193741750084 1113 10 -4.572005575548154 1113 11 -5.493557202409413 1113 12 -3.4364988140868817 1113 13 -2.2594649465358856 1113 14 -2.740272671305174 1113 15 -2.9665418030127277 1113 16 -3.2221439045564253 1113 17 -2.898411520019131 1113 18 -3.8105047401490175 1113 19 -3.2723399506338673 1113 20 -4.096627163563415 1113 21 -1.389912921597567 1113 22 -2.4406622194627055 1113 23 -2.898523056152225 1113 24 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24 3.1745845031906796 1203 25 1.687359337432195 1204 1 3.9354140370419195 1204 2 -14.08941500982171 1204 3 -5.965731480533034 1204 4 1.0874265768775757 1204 5 4.127070386533106 1204 6 4.386713165576193 1204 7 4.526555280797563 1204 8 5.023493372521685 1204 9 3.560729456514732 1204 10 3.052152300162515 1204 11 5.846776032333757 1204 12 3.433411468866879 1204 13 2.4658135706680966 1204 14 3.776684836335562 1204 15 3.757109976539674 1204 16 3.693446486189245 1204 17 4.1415174950651625 1204 18 4.2528723014888925 1204 19 2.6110278892557126 1204 20 3.306918795883167 1204 21 4.886040938207227 1204 22 1.30827793630975 1204 23 2.932559135178412 1204 24 4.515935525626418 1204 25 2.1214989730509863 1205 1 2.7344996133014092 1205 2 2.4820135616068626 1205 3 2.6318933181787223 1205 4 -19.828837513299558 1205 5 -6.874626185815641 1205 6 -0.21695028047855658 1205 7 2.8501117565279515 1205 8 3.2803084173802084 1205 9 2.1777929485782224 1205 10 1.7892410970339192 1205 11 4.950985832195734 1205 12 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1.1263046333051854 2204 6 3.8803996731949617 2204 7 5.071140547578363 2204 8 5.060579341507726 2204 9 3.6191599229442346 2204 10 2.9453313135673262 2204 11 4.6910771125224215 2204 12 4.615078002082775 2204 13 2.275049507816597 2204 14 3.719893907937621 2204 15 3.960767290213802 2204 16 3.912805871654804 2204 17 -1.5935636620673996 2204 18 2.396420210950808 2204 19 3.08125120958713 2204 20 3.4667273639309855 2204 21 5.245980542201597 2204 22 4.452654511505447 2204 23 3.302695648961336 2204 24 5.049018606991986 2204 25 5.1832174122616195 2205 1 6.167482904418993 2205 2 10.499720414814863 2205 3 7.276894316149512 2205 4 7.8903667713217125 2205 5 7.844344644923758 2205 6 7.547158531603543 2205 7 7.409013418386934 2205 8 7.299674397031108 2205 9 5.818481287051899 2205 10 5.323085969602552 2205 11 7.706069542936273 2205 12 6.7861627264948865 2205 13 9.847439449898847 2205 14 7.236148741210464 2205 15 6.33973271770671 2205 16 5.798277157651096 2205 17 6.6148883077460425 2205 18 6.377337124250513 2205 19 4.8245012341950755 2205 20 5.2647163359467655 2205 21 -1.2486851188101724 2205 22 3.8386569064778158 2205 23 5.236846590192723 2205 24 7.214850817619119 2205 25 7.451654200631452 2206 1 4.029368821985802 2206 2 8.671159008305178 2206 3 6.014807412491081 2206 4 2.301071852259355 2206 5 5.678443281824297 2206 6 5.957077697979344 2206 7 6.115592794601923 2206 8 5.906847138740286 2206 9 4.69014259485996 2206 10 3.9908816179473945 2206 11 3.7647161056002467 2206 12 4.747340858653356 2206 13 7.995262466562547 2206 14 0.6288529114118191 2206 15 2.547767769496165 2206 16 3.507287090224253 2206 17 4.293133152493244 2206 18 -1.2148084687335796 2206 19 0.9079578482439921 2206 20 2.360881072884979 2206 21 3.489351065620859 2206 22 -2.2703667796001703 2206 23 -2.962064340260774 2206 24 1.1149425847017795 2206 25 2.8804468740585243 2207 1 1.7380505975495453 2207 2 -14.08941500982171 2207 3 -6.166167878495669 2207 4 -4.19169017787636 2207 5 1.4923372480518395 2207 6 2.2400996036702807 2207 7 3.434882682753152 2207 8 3.6145463308909846 2207 9 0.9297911377953021 2207 10 0.7665211258506801 2207 11 -1.311913562262884 2207 12 -1.8054653048674645 2207 13 0.793721586357222 2207 14 -5.352231765969346 2207 15 -1.2368665200605093 2207 16 0.04377763374677812 2207 17 0.9299868967279679 2207 18 1.0383192244920387 2207 19 0.4317576758775523 2207 20 0.3783994696876789 2207 21 -3.871216796561754 2207 22 -4.0503912139636 2207 23 -1.0269787726353563 2207 24 0.6869434560046628 2207 25 1.2105806948163682 2208 1 4.2603349099388055 2208 2 6.2846479586940305 2208 3 5.739277126274672 2208 4 7.767864135875854 2208 5 3.2002560159406244 2208 6 5.751752867947232 2208 7 6.190334502297922 2208 8 6.275034268740242 2208 9 4.34000648466732 2208 10 4.0459609513924555 2208 11 6.39733408663994 2208 12 5.575173920974159 2208 13 8.084337641965782 2208 14 2.714382654093349 2208 15 4.156243742989783 2208 16 4.621704579364877 2208 17 5.3604080497878 2208 18 0.5360362750576648 2208 19 3.1158588071301097 2208 20 4.012343861128322 2208 21 6.539714101625517 2208 22 5.650440188516978 2208 23 1.0035514168153732 2208 24 2.6732457593984194 2208 25 4.017245544209853 2209 1 3.314644954916318 2209 2 5.239384990178291 2209 3 5.596667231222199 2209 4 1.205562486700444 2209 5 5.421862012942121 2209 6 5.877145371695356 2209 7 6.563736601248447 2209 8 4.699810280734244 2209 9 4.663585495162074 2209 10 3.6384646995183942 2209 11 -0.43124522370488805 2209 12 3.1906713722485236 2209 13 2.5162908653540867 2209 14 3.8529205851278334 2209 15 4.271262633544506 2209 16 3.7393801415837693 2209 17 1.3444044515869003 2209 18 3.496680353018686 2209 19 4.295545763651848 2209 20 4.1788170245466105 2209 21 6.623780068642009 2209 22 5.094082767227622 2209 23 0.11507765369693246 2209 24 -3.471413910766497 2209 25 -0.16501052775416625 2210 1 -2.4342267272476725 2210 2 -14.08941500982171 2210 3 -8.050569286106626 2210 4 -2.836445290138105 2210 5 -1.1917041747163744 2210 6 -1.8198987534103068 2210 7 -1.799746746173362 2210 8 -21.176989719265755 2210 9 -13.625199627407664 2210 10 -7.927722958976958 2210 11 -10.322489855995675 2210 12 -5.2668123132967 2210 13 -4.837409219176767 2210 14 -4.538233430087722 2210 15 -6.398189944646536 2210 16 -5.84750946112122 2210 17 -5.369673654921975 2210 18 -5.720166984259421 2210 19 -10.842077904987205 2210 20 -7.706341386273337 2210 21 -5.589684005745141 2210 22 -10.243931095832393 2210 23 -8.20826300564373 2210 24 -10.719586353708172 2210 25 -8.37711940349381 2211 1 0.16743944717104853 2211 2 5.027624577097395 2211 3 0.7287746322549005 2211 4 1.5188389066660193 2211 5 1.1787955757648412 2211 6 0.18671094828744117 2211 7 -20.913863398751552 2211 8 -13.242394194308785 2211 9 -9.126620356817273 2211 10 -7.143979362443741 2211 11 -9.518744018885611 2211 12 -4.956217440143906 2211 13 -6.578047172511662 2211 14 -4.819654974252547 2211 15 -5.198921187453774 2211 16 -11.961605744818986 2211 17 -8.09607885306026 2211 18 -6.170191936137943 2211 19 -9.132064563370527 2211 20 -6.938974369601407 2211 21 -4.4880891224079065 2211 22 -6.080328248262909 2211 23 -8.20296709673336 2211 24 -22.5074139107665 2211 25 -22.270610527754165 2212 1 -0.9684468800266153 2212 2 -14.08941500982171 2212 3 -7.80054646500256 2212 4 -19.828837513299558 2212 5 -20.311737987057878 2212 6 -21.233254628304643 2212 7 -9.89367024686393 2212 8 -9.466717550696835 2212 9 -5.163668587366459 2212 10 -5.635810980903031 2212 11 -5.570120728533684 2212 12 -2.914285256373077 2212 13 -6.623952857209565 2212 14 -3.8999341997712023 2212 15 -3.7881220898971115 2212 16 -3.9507543623670642 2212 17 -4.091219077824864 2212 18 -3.193383913882105 2212 19 -3.8925581871822175 2212 20 -3.9033420623717383 2212 21 -1.7994192289874924 2212 22 -3.2002132819383107 2212 23 -3.2943130398939395 2212 24 -2.2326114524697473 2212 25 -2.6823752336365168 2213 1 -19.059755045083683 2213 2 -14.08941500982171 2213 3 -8.78338559896648 2213 4 -5.987328079337294 2213 5 -20.311737987057878 2213 6 -11.080424439625398 2213 7 -20.913863398751552 2213 8 -10.843027455114813 2213 9 -8.770844693517173 2213 10 -7.420969262745757 2213 11 -6.635554657667154 2213 12 -5.474445608883553 2213 13 -19.522109134645913 2213 14 -12.708649226192922 2213 15 -7.540027932493231 2213 16 -6.616212311246418 2213 17 -5.328621963507437 2213 18 -5.286081911132261 2213 19 -6.225035368423622 2213 20 -6.251907503755273 2213 21 -4.281842572867426 2213 22 -5.086604025225206 2213 23 -4.899865742529485 2213 24 -3.967791269257063 2213 25 -4.77155392398058 2214 1 -19.059755045083683 2214 2 -14.08941500982171 2214 3 -12.538582341427373 2214 4 -19.828837513299558 2214 5 -20.311737987057878 2214 6 -21.233254628304643 2214 7 -16.630743740631893 2214 8 -15.194083736359772 2214 9 -14.217373479196901 2214 10 -14.124996838943146 2214 11 -14.924173428833093 2214 12 -12.515027773050623 2214 13 -19.522109134645913 2214 14 -17.07750505589781 2214 15 -24.281537366455495 2214 16 -18.621941225937597 2214 17 -24.0323955484131 2214 18 -18.234384604246273 2214 19 -15.36574654404046 2214 20 -13.994239385709799 2214 21 -11.478972068110128 2214 22 -12.574247147302291 2214 23 -12.539306961687684 2214 24 -11.34929425264684 2214 25 -11.062277194420831 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 117014.0 3 5.0 4 522.0 5 2153.0 6 3076.0 7 2804.0 8 2483.0 9 2281.0 10 2167.0 11 1964.0 12 1990.0 13 2214.0 14 3299.0 15 6944.0 16 10607.0 17 11110.0 18 11327.0 19 13132.0 20 15688.0 21 18546.0 22 19449.0 23 21034.0 24 22542.0 25 23202.0 26 19020.0 27 15212.0 28 16409.0 29 18909.0 30 18256.0 31 21501.0 32 27758.0 33 34989.0 34 42693.0 35 48941.0 36 48766.0 37 49665.0 38 35781.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.463893229500215 18.5932576786126 12.598464277480339 25.344384814406844 2 13.444385579420015 20.08797908651132 41.46220720681599 25.00542812725267 3 19.532269604931212 24.180242264612687 29.366695948578396 26.920792181877708 4 11.1000504174338 17.433962021014242 38.914527147291395 32.55146041426056 5 11.984218815286214 39.19037058464308 36.00970655353068 12.81570404654003 6 31.582830860244766 37.765443937005315 17.7864096604853 12.865315542264625 7 27.20246342391882 32.362800202819436 22.163417599980274 18.271318773281468 8 23.86769707830293 37.80527756348611 19.95790992905981 18.369115429151147 9 25.791537754579448 16.221525336925332 20.396663369323484 37.59027353917174 10 14.83013117738699 29.38887555544826 34.52463286095083 21.25636040621392 11 32.82283167668459 22.187783814376324 24.980554316793512 20.008830192145574 12 24.09365547741072 24.892803526131683 31.72453575311162 19.28900524334598 13 28.322031714994434 21.3716190105001 25.148550497260196 25.157798777245265 14 19.980772129827944 22.621902136967286 28.69361412850247 28.7037116047023 15 22.597357691365673 31.80308029218113 23.46021409327321 22.13934792317999 16 20.886463731970505 28.62777605582026 28.834362903748982 21.651397308460258 17 21.207163953855083 28.614918039633018 28.91891230247933 21.259005704032568 18 20.71189547498774 28.20797010323195 30.723049925044936 20.35708449673537 19 22.339642899749474 27.69557856944026 27.082753409949493 22.882025120860767 20 22.693292773783988 27.649420423907266 27.961348473815757 21.69593832849299 21 21.90828742049046 26.597759619027006 26.69736135832786 24.796591602154674 22 22.06476667354211 28.16916434916819 27.56075876441176 22.20531021287794 23 21.437601817383285 28.58800599994668 28.51139302316771 21.462999159502324 24 23.281027104136946 27.212430782445967 28.313009065390453 21.193533048026627 25 21.83599987651861 27.750197149263744 28.36886697406049 22.044936000157158 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 661.0 1 661.0 2 1399.5 3 2138.0 4 2138.0 5 2138.0 6 3068.5 7 3999.0 8 3999.0 9 3999.0 10 4682.5 11 5366.0 12 5366.0 13 5366.0 14 6757.5 15 8149.0 16 8149.0 17 8149.0 18 11609.0 19 15069.0 20 15069.0 21 15069.0 22 21312.5 23 27556.0 24 27556.0 25 27556.0 26 36215.0 27 44874.0 28 44874.0 29 44874.0 30 54498.5 31 64123.0 32 64123.0 33 64123.0 34 70788.5 35 77454.0 36 77454.0 37 77454.0 38 81747.0 39 86040.0 40 86040.0 41 86040.0 42 88306.0 43 90572.0 44 90572.0 45 90572.0 46 91440.5 47 92309.0 48 92309.0 49 92309.0 50 83252.5 51 74196.0 52 74196.0 53 74196.0 54 62706.0 55 51216.0 56 51216.0 57 51216.0 58 42369.0 59 33522.0 60 33522.0 61 33522.0 62 26539.5 63 19557.0 64 19557.0 65 19557.0 66 14726.0 67 9895.0 68 9895.0 69 9895.0 70 7149.5 71 4404.0 72 4404.0 73 4404.0 74 3024.5 75 1645.0 76 1645.0 77 1645.0 78 1071.5 79 498.0 80 498.0 81 498.0 82 331.5 83 165.0 84 165.0 85 165.0 86 99.0 87 33.0 88 33.0 89 33.0 90 22.0 91 11.0 92 11.0 93 11.0 94 6.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 12.45506010907516 2 35.44578269346404 3 5.383115636208692 4 12.984176953492382 5 12.781921163692633 6 13.758860079080193 7 9.054555801152985 8 7.157303984985697 9 0.10624385909092819 10 1.3023983359800855 11 5.395730342433209 12 5.236224390394321 13 15.128536848257697 14 1.4446641895121333 15 2.7139839625034865 16 2.77495504258865 17 2.6675898762777646 18 1.3593046773929047 19 1.3087056890923439 20 0.29027840656637505 21 4.167057956165297 22 1.4672304973137684 23 0.10932745394581003 24 8.623273011677012 25 0.11142990498322945 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 713453.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.65994634785125 #Duplication Level Percentage of deduplicated Percentage of total 1 96.88133871295128 88.80138308537119 2 2.2277781542962534 4.083960521954193 3 0.407812547502673 1.1214022867222664 4 0.15588168625926183 0.5715242799654608 5 0.08587901803958375 0.39358330929571983 6 0.05586299451984595 0.30722394483116333 7 0.030467255982675596 0.19548389341168065 8 0.025017606109368404 0.18344899469891068 9 0.016752215167296483 0.138195642909785 >10 0.09586245252547027 1.6156478569779915 >50 0.00772765185641533 0.5060795752813644 >100 0.008515804248607234 1.3688677323991432 >500 9.462004640317914E-4 0.5335259529761948 >1k 1.577000773386319E-4 0.17967292320493364 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGA 1243 0.1742231093008229 No Hit GCTTACTCTGCGTTGATACCACTGC 802 0.11241104880069185 No Hit GTCCTACAGTGGACATTTCTAAATT 718 0.10063732299114307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 1.4016340249462824E-4 0.0 0.0 0.0 13 0.0 1.4016340249462824E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCTTAG 30 8.16121E-5 26.478813 1 GCCTAAC 30 0.0025333592 22.065678 1 ATTGGAC 45 2.4269825E-4 16.904667 3 GTTCTAG 105 3.4615368E-9 16.391647 1 TAAGACG 40 0.0021539796 16.30093 3 GTATAAT 60 4.8233944E-4 15.445973 1 GTTCTAA 70 9.03433E-5 15.130751 1 GTGTAGA 105 6.144546E-8 15.130751 1 TTGGACT 50 6.285712E-4 14.917714 4 TCGTCAA 55 0.0041262354 14.784866 2 CGTCAAG 45 0.004719919 14.489715 3 CAGTAGG 55 0.0048202933 14.442989 1 TGTAGAA 235 0.0 13.264438 2 GTATAAG 70 0.0015486736 13.239407 1 CTTTAGA 60 0.008577147 13.239407 1 CTAGAGT 65 3.045322E-4 13.114474 4 TATACTG 135 8.54925E-11 13.04808 5 TAGGACC 200 0.0 12.7866125 4 GTAGGAC 520 0.0 12.539177 3 GTCTTGT 75 0.0026013085 12.356779 1 >>END_MODULE