FastQCFastQC Report
Thu 26 May 2016
SRR1926716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926716_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713453
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGA34280.4804801437515856No Hit
GCTTACTCTGCGTTGATACCACTGC16600.23267124814108286No Hit
GTCCTACAGTGGACATTTCTAAATT14830.20786232589953368No Hit
CTGTAGGACGTGGAATATGGCAAGA12810.17954931859561876No Hit
GTCCTACAGTGTGCATTTCTCATTT12150.1702985340309733No Hit
CTTTAGGACGTGAAATATGGCGAGG11710.16413134432120968No Hit
GCGTTGATACCACTGCTTACTCTGC11260.15782399120895138No Hit
CTGCTTACTCTGCGTTGATACCACT10840.15193712830417702No Hit
GTCCTAAAGTGTGTATTTCTCATTT10660.1494141870592737No Hit
GTATCAACGCAGAGTACTTTTTTTT10300.1443683045694671No Hit
CCCATGTACTCTGCGTTGATACCAC10000.14016340249462825No Hit
CTGTAGGACCTGGAATATGGCGAGA9850.1380609514572088No Hit
GTACATGGGAAGCAGTGGTATCAAC8560.11997987253540177No Hit
GTATCAACGCAGAGTAAGCAGTGGT8390.11759709469299309No Hit
ACGCAGAGTAAGCAGTGGTATCAAC7410.10386108124851952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTC551.898942E-413.8679825
TAGGACC3150.013.3176664
GATATAC2300.013.2659631
TCTGGAC751.4192114E-512.7150083
GTATAAG751.4212723E-512.7132151
GTCCTAC8200.012.3256171
AGGACCT4900.012.25830655
TAGGGCC550.002984115612.1344844
TCCTACA8850.011.74770452
TTAGGAC5850.011.7369313
TTAGTAC657.761921E-411.7369313
ACTGGTA657.994669E-411.6956818
TAGGACG9450.011.400734
GTCCTAA3100.011.3803781
TGTAGGA9400.011.3647532
GTAGGAC9250.011.3404133
ACTGTTC2850.011.3366038
CTACAGT10600.011.3331524
GGCGAGG4350.011.32616219
CTGTAGG9350.011.319521
Produced by FastQC (version 0.11.5)