FastQCFastQC Report
Thu 26 May 2016
SRR1926715_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926715_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680994
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10360.1521305620901212No Hit
GTCCTAAAGTGTGTATTTCTCATTT8990.13201291053959358No Hit
CTGTAGGACGTGGAATATGGCAAGA7600.11160157064526266No Hit
CTTTAGGACGTGAAATATGGCGAGG7320.10748993383201615No Hit
GTCCTACAGTGTGCATTTCTCATTT7220.10602149211299952No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAC350.00136079117.4591837
TAGGACC2550.016.8132274
GGCGTGC350.001816509816.7220758
GTTTAGG808.459592E-716.682911
TTTAGAA2000.015.7292342
CTTAGGC505.1108486E-415.3294863
GTCTAGA801.561698E-515.014621
GTCCTAA3650.014.9917671
GTCTTAT751.5174055E-414.2360841
TGTAGGA6450.014.2077332
TTTGGCG350.005665374514.097198516
GCAGGAC550.00105224713.9358983
TTGGCGA350.006335030313.86158317
TCTTATA700.001211155113.6775952
AGGACCT4650.013.6697275
GGACCTG4600.013.5862876
CTGTAGG6800.013.5425981
GTCCTAC7850.013.4313361
TTTAGGA5100.013.4094072
GTCCTGA700.001457295613.3463291
Produced by FastQC (version 0.11.5)