FastQCFastQC Report
Thu 26 May 2016
SRR1926715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926715_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences680994
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT21280.3124843978067354No Hit
CTGTAGGACGTGGAATATGGCAAGA20660.3033800591488325No Hit
GTCCTAAAGTGTGTATTTCTCATTT18980.2787102382693533No Hit
CTTTAGGACGTGAAATATGGCGAGG18250.26799061372053207No Hit
GTCCTACAGTGTGCATTTCTCATTT15140.22232207625911535No Hit
GTATCAACGCAGAGTACTTTTTTTT14340.21057454250698243No Hit
CTGTAGGACCTGGAATATGGCGAGA12970.19045689095645485No Hit
CTGAAGGACCTGGAATATGGCGAGA10530.15462691301244946No Hit
CCCATGTACTCTGCGTTGATACCAC8910.1308381571643803No Hit
GTACATGGGAAGCAGTGGTATCAAC8640.12687336452303544No Hit
GGTATCAACGCAGAGTACTTTTTTT8180.12011853261555902No Hit
TATCAACGCAGAGTACTTTTTTTTT6850.10058825775263805No Hit
GTCCTTCAGTGTGCATTTCTCATTT6820.10014772523693306No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGCG402.817923E-416.57841916
CTGTGCG350.002167609316.2867249
GTCGAGG651.9705294E-716.03187819
TATGTCG901.4006218E-1015.78897216
TGTCGAG901.4006218E-1015.78897218
ATTGGAC456.5512303E-414.8376963
TAGGACC4150.014.7078294
ATGTCGA1006.85759E-1014.21007317
TTGGACA603.9544943E-412.7161454
GGACCTG7650.012.5905456
AGGACCT7850.012.1491825
CCTGGTA550.00306396512.0916599
ATAGGAC959.874202E-712.04865553
TATACTG959.891864E-712.0468735
GGCGAGG5800.011.92339619
CTGTAGG12050.011.5536271
GTAGACA751.9968793E-411.44453054
TGTAGGA12300.011.4013792
TCCAACG752.125212E-411.36805918
ACTGTTC2600.011.3276258
Produced by FastQC (version 0.11.5)