##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926715_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 680994 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.458833704849088 31.0 28.0 31.0 23.0 34.0 2 28.902602666102784 31.0 28.0 33.0 23.0 34.0 3 29.283503232040225 31.0 30.0 33.0 25.0 34.0 4 33.017226289805784 35.0 33.0 37.0 27.0 37.0 5 32.757262178521394 35.0 33.0 37.0 26.0 37.0 6 32.84620128811708 35.0 33.0 37.0 26.0 37.0 7 32.66869164779719 35.0 33.0 37.0 25.0 37.0 8 32.631961515079425 35.0 33.0 37.0 25.0 37.0 9 33.83742881728767 38.0 34.0 39.0 23.0 39.0 10 33.74457190518566 37.0 33.0 39.0 23.0 39.0 11 34.00035683133772 37.0 34.0 39.0 25.0 39.0 12 33.79445487038065 37.0 34.0 39.0 25.0 39.0 13 33.945224186997244 38.0 34.0 39.0 25.0 39.0 14 34.59544577485264 39.0 34.0 40.0 22.0 41.0 15 34.73762177052955 39.0 34.0 40.0 25.0 41.0 16 34.725545305832355 39.0 34.0 40.0 25.0 41.0 17 34.64512903197385 39.0 34.0 40.0 24.0 41.0 18 34.63051069466104 39.0 34.0 40.0 24.0 41.0 19 34.66325694499511 39.0 34.0 40.0 23.0 41.0 20 34.664519804873464 39.0 34.0 41.0 23.0 41.0 21 34.576220935867276 39.0 34.0 41.0 22.0 41.0 22 34.52016757856897 39.0 34.0 40.0 19.0 41.0 23 34.17649347865033 39.0 34.0 40.0 18.0 41.0 24 34.25802576821528 39.0 34.0 41.0 18.0 41.0 25 34.06648076194504 39.0 34.0 40.0 17.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7372629107434925 1101 2 0.7427555695011883 1101 3 0.6152190354709184 1101 4 0.4907508482287639 1101 5 0.6367327868059647 1101 6 0.23307622950808593 1101 7 0.632636522037977 1101 8 0.6422927656986559 1101 9 0.9510224094265212 1101 10 0.9503776820843939 1101 11 0.4991143163007479 1101 12 0.5726810132357585 1101 13 0.5655526860687701 1101 14 0.908138571755245 1101 15 0.5361431257449993 1101 16 0.7410150573218743 1101 17 0.7686429643865083 1101 18 0.6279565790262538 1101 19 0.9496078353057413 1101 20 0.8017690304618696 1101 21 0.6270646432707707 1101 22 0.6534267565780496 1101 23 0.8520687953318884 1101 24 0.6936861552978328 1101 25 0.9028975116537268 1102 1 0.41620060672868675 1102 2 0.29310127453383217 1102 3 0.4545026078419312 1102 4 0.11853962116867223 1102 5 0.24583335398497752 1102 6 -0.06313558015280307 1102 7 0.27621977397855346 1102 8 0.3917829899999603 1102 9 0.44545557312020634 1102 10 0.48634257181862495 1102 11 0.4097879056357243 1102 12 0.3905368709440751 1102 13 0.2551164208825938 1102 14 0.6799496891493035 1102 15 0.5301350550104047 1102 16 0.2977907902850845 1102 17 0.45056220563463967 1102 18 0.473828681015668 1102 19 0.25626362104572564 1102 20 0.4538792448246909 1102 21 0.4457729830298476 1102 22 0.30109892368166413 1102 23 0.38118812568367133 1102 24 0.36846425293487073 1102 25 0.4501460080296056 1103 1 -0.17650992576017188 1103 2 -0.31411782000053634 1103 3 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1.0071219096195918 2202 18 0.9703262464501066 2202 19 0.7956495304482445 2202 20 0.7808932545629546 2202 21 0.8739225374252513 2202 22 0.9500029297285266 2202 23 1.1350047686199147 2202 24 0.9943563410362657 2202 25 0.8452992889802289 2203 1 -0.12772958800755063 2203 2 0.06564932140646107 2203 3 -0.07286757305826086 2203 4 -0.33710691041452634 2203 5 -0.1556455248100761 2203 6 -0.02824736081511503 2203 7 0.4194205422430386 2203 8 0.28445959677112853 2203 9 0.493201299421429 2203 10 0.4547267586780279 2203 11 0.4290369968868575 2203 12 0.4097844450852435 2203 13 0.2518379462456366 2203 14 0.6218163109095158 2203 15 0.6188321306292366 2203 16 0.4947336619921998 2203 17 0.5661803976036666 2203 18 0.40923468998482093 2203 19 0.410143326362288 2203 20 0.6415973293823356 2203 21 0.6354542078837468 2203 22 0.3374215704923955 2203 23 0.6027142472830747 2203 24 0.2611992684397393 2203 25 0.2778303824037991 2204 1 0.1665481119159793 2204 2 0.24708248468994043 2204 3 0.12934901199407633 2204 4 -0.36583853961136015 2204 5 -0.18234216431070394 2204 6 -0.32120478064523184 2204 7 -0.0329565303579642 2204 8 -0.1494032381021526 2204 9 0.4308495182938614 2204 10 0.1472129097583874 2204 11 -0.14023261234206785 2204 12 -0.013998180119678238 2204 13 -0.07636306980918306 2204 14 0.39769383863577445 2204 15 0.4229221188588852 2204 16 0.2400666386023218 2204 17 0.14554006933747132 2204 18 0.22741499234563634 2204 19 0.3659647232368144 2204 20 0.36378220345929435 2204 21 0.33282585005601106 2204 22 0.2979056327714176 2204 23 0.5167202059754317 2204 24 0.4070561127884176 2204 25 0.3070266107379851 2205 1 0.508212537128987 2205 2 0.4690191291128478 2205 3 0.5594405629234629 2205 4 0.3873539308121252 2205 5 0.2815732780953226 2205 6 0.32174374846306364 2205 7 0.3949725514714615 2205 8 0.42899284881786315 2205 9 0.8521189922567061 2205 10 0.7000770605890096 2205 11 0.20565499619053895 2205 12 0.44624910558668063 2205 13 0.39018803746892416 2205 14 0.49328934662032964 2205 15 0.4125360053402858 2205 16 0.6269835226544132 2205 17 0.5274474823583972 2205 18 0.6375469321352085 2205 19 0.7320823404933066 2205 20 0.6433298994584078 2205 21 0.7423408564103582 2205 22 0.6925117702273198 2205 23 0.832932391229086 2205 24 0.7919208214919422 2205 25 0.8386488966222316 2206 1 0.1577305520401424 2206 2 0.2964692884060476 2206 3 0.14251114458996383 2206 4 0.12881039614532597 2206 5 0.46625485591908244 2206 6 0.3593500807782348 2206 7 0.4059383280668527 2206 8 0.39435068374412907 2206 9 0.7386061666909711 2206 10 0.8005015687507324 2206 11 0.5642330170136063 2206 12 0.5264745793607801 2206 13 0.40440947143614636 2206 14 0.7695521968190349 2206 15 0.7106072438859528 2206 16 0.5468237367970232 2206 17 0.7883639930162616 2206 18 0.7439367242329524 2206 19 0.6481537104148103 2206 20 0.796099466246595 2206 21 1.0298732898192284 2206 22 0.8800138229747176 2206 23 0.7676427075164085 2206 24 0.5860179410767969 2206 25 0.7151186637585667 2207 1 -1.134078200446357 2207 2 -0.39104847232143314 2207 3 0.09910392663031686 2207 4 -0.017573519739869425 2207 5 -0.06713531174218446 2207 6 0.055570597970621805 2207 7 0.12080760911260313 2207 8 0.03366583009252366 2207 9 0.535458965781622 2207 10 0.34181302086781074 2207 11 -0.0947965368369168 2207 12 -0.4207050171154947 2207 13 -0.4077530747417413 2207 14 -5.139619442851323 2207 15 -2.383275961570142 2207 16 -1.2010804973604081 2207 17 -1.1419073908996253 2207 18 -0.676992517028765 2207 19 -0.662197241560186 2207 20 -0.40278516006770104 2207 21 -0.3352872671571845 2207 22 -0.2843439491196307 2207 23 -0.1052260051860685 2207 24 -0.41266776508972924 2207 25 -0.40036158631247787 2208 1 0.424693795837797 2208 2 0.6782250197885382 2208 3 0.7022831451969864 2208 4 0.5875100355954501 2208 5 0.553864813165923 2208 6 0.6762048718005929 2208 7 0.9057335891122946 2208 8 0.8399319853867482 2208 9 1.0227439344214915 2208 10 1.1898175967365177 2208 11 1.12771190469892 2208 12 1.0133902556926913 2208 13 0.9436277741829215 2208 14 1.3327079501798949 2208 15 1.0114459540697922 2208 16 1.151500288495896 2208 17 0.9214933723367125 2208 18 0.9204975807613138 2208 19 0.8364304756178171 2208 20 0.9060586754440223 2208 21 1.2628021165407404 2208 22 1.232747644018481 2208 23 1.6054089989809484 2208 24 1.2930849018349448 2208 25 1.297254306913615 2209 1 -0.4333327963761455 2209 2 -0.40349300420330536 2209 3 0.2735066197030882 2209 4 0.42253840016744704 2209 5 0.625983362706414 2209 6 0.4928342436741602 2209 7 0.8816924758798237 2209 8 0.8403788385958109 2209 9 0.8669495553284747 2209 10 0.640986737177073 2209 11 0.5527322628657956 2209 12 0.5339783500998436 2209 13 0.6405080134170404 2209 14 0.5844717543856532 2209 15 0.7268339909950541 2209 16 0.600054003262585 2209 17 0.6660035103616622 2209 18 0.8491906206633217 2209 19 0.9445463700427652 2209 20 0.8421401902284984 2209 21 0.7292196950479877 2209 22 0.8562707359963326 2209 23 1.1203953470337495 2209 24 0.9765533860240083 2209 25 1.0900683872377002 2210 1 -0.5129946087112245 2210 2 -0.5343807083583378 2210 3 -0.04155873298602941 2210 4 0.16836067470259763 2210 5 0.8053005251918961 2210 6 0.4288393826495991 2210 7 0.7087428732272514 2210 8 0.676618616419745 2210 9 0.8769913287365867 2210 10 0.8617834322311992 2210 11 0.6918038623334368 2210 12 0.6668231730436744 2210 13 0.5114966507423162 2210 14 0.9643635897230354 2210 15 0.5655317069204955 2210 16 0.7174003532822013 2210 17 0.7797418072682731 2210 18 0.805853209999178 2210 19 0.6228857424415395 2210 20 0.734594050999192 2210 21 0.9030370767861626 2210 22 0.899031165885475 2210 23 0.8907454589680199 2210 24 0.7505792501488813 2210 25 0.9509106253076638 2211 1 -1.1059195345076134 2211 2 -0.14647938501076752 2211 3 0.5308283551470545 2211 4 0.6112902643843228 2211 5 0.88200828454179 2211 6 0.7332595500870696 2211 7 0.8300939808800933 2211 8 0.931978696271841 2211 9 1.1026675721802377 2211 10 1.2067267839622957 2211 11 0.8841767754440539 2211 12 0.9489730886552579 2211 13 0.8486628740076867 2211 14 1.0839456099268219 2211 15 0.7683214278766499 2211 16 0.9941967663158877 2211 17 0.9319789973291748 2211 18 1.0392483829974353 2211 19 1.0626805633762473 2211 20 1.0482798039854728 2211 21 1.2279895011694322 2211 22 1.2883882592564646 2211 23 1.4941206769149744 2211 24 1.4305872863820852 2211 25 1.4654001610637195 2212 1 0.9618808788375297 2212 2 0.1876522949419943 2212 3 0.503386961583427 2212 4 0.4036666052956548 2212 5 0.7152483199713728 2212 6 0.6214154402553618 2212 7 0.8948206810080279 2212 8 0.7764814026983728 2212 9 1.1279068202857374 2212 10 0.9049525491428909 2212 11 0.6454160235495578 2212 12 0.9532262133477047 2212 13 0.7757558766649026 2212 14 0.892369190283091 2212 15 0.7200647602258243 2212 16 0.31913092633950413 2212 17 0.8403966727548209 2212 18 0.6938060052787094 2212 19 0.9140164555128507 2212 20 1.0585504466387547 2212 21 1.0403308061590977 2212 22 1.1185955223211224 2212 23 1.266002184640591 2212 24 1.1786388561094796 2212 25 1.0083931735624887 2213 1 0.37755111577358846 2213 2 0.2906214607972366 2213 3 -0.17143549865675212 2213 4 -0.8533676909285148 2213 5 -1.793674756951706 2213 6 -0.4727072471837843 2213 7 -0.2864131175422244 2213 8 -0.006023844515731014 2213 9 -0.34957105486302 2213 10 -0.3138655834980284 2213 11 -0.052969349164932567 2213 12 -0.3080750957510716 2213 13 -0.41425775527277864 2213 14 -6.838010123792426 2213 15 -2.8766280884163926 2213 16 -1.4049768308821768 2213 17 -0.7162857896491559 2213 18 -5.022463604155462 2213 19 -2.5495897329541037 2213 20 -5.3011466214127445 2213 21 -2.0947552048762788 2213 22 -1.2870052566467436 2213 23 -3.55617070295002 2213 24 -1.347183495940726 2213 25 -0.7861947307975683 2214 1 0.6351924019200439 2214 2 0.576095770041956 2214 3 -1.0887933364899993 2214 4 -1.4492993464794601 2214 5 -0.2565553877712716 2214 6 -0.12029012135860029 2214 7 -0.2764169295578256 2214 8 -0.2561632113813559 2214 9 -5.456885134155069 2214 10 -1.325758222697658 2214 11 -0.6633802929414188 2214 12 -0.6654297905296715 2214 13 -1.029286653324192 2214 14 -2.0684469977697404 2214 15 -1.5558921039447782 2214 16 -0.9401551514503907 2214 17 -1.1221061746945864 2214 18 -0.8636979326832446 2214 19 -3.442332993172478 2214 20 -1.3864125971029182 2214 21 -3.431933824884158 2214 22 -5.632976996762849 2214 23 -5.170617711154879 2214 24 -4.8272373101331 2214 25 -2.555364974989555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 22126.0 3 9.0 4 380.0 5 2505.0 6 3246.0 7 2750.0 8 2003.0 9 1655.0 10 1436.0 11 1198.0 12 1261.0 13 1192.0 14 1262.0 15 1267.0 16 1269.0 17 1349.0 18 1463.0 19 1741.0 20 2341.0 21 3149.0 22 3940.0 23 4779.0 24 5866.0 25 6820.0 26 8022.0 27 8944.0 28 10126.0 29 12230.0 30 15196.0 31 18995.0 32 24888.0 33 32472.0 34 41753.0 35 56571.0 36 77319.0 37 114758.0 38 184713.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.166459437453526 20.36857405725405 9.591332233989707 29.873634271302723 2 12.451268702976318 22.025077338441246 41.63657836521371 23.887075593368724 3 17.692205999001146 26.245336231969212 29.506154704897327 26.55630306413232 4 11.444087654716332 18.744877064230963 38.71705529979641 31.093979981256297 5 11.946794119504661 40.01912380090683 35.908241006893086 12.125841072695414 6 30.222797492072427 39.64551124896621 18.295252008980892 11.836439249980467 7 26.97821662106693 32.17820543576324 22.84289210744367 18.000685835726156 8 23.509292454773973 40.57361849425145 20.133308608398238 15.78378044257634 9 26.230502268050948 15.572723338994876 20.25630005975527 37.94047433319891 10 14.30696813705692 30.66861511121555 34.79721245144187 20.22720430028566 11 33.479300298395245 22.799188223960904 25.754064754117618 17.967446723526233 12 23.584117385321843 25.370096523934205 32.51768430667386 18.5281017840701 13 29.26809927840774 23.326637239094616 25.70624704476104 21.699016437736603 14 19.732806647062887 24.252256859486156 27.88409962952676 28.1308368639242 15 21.566681008721243 33.99184411899891 23.130307600358893 21.31116727192095 16 20.33117270262888 29.12034820712685 30.232872326990456 20.31560676325381 17 20.184767476694805 30.44022885203917 28.927667248195206 20.447336423070823 18 20.03289425378504 28.54266707784484 31.884077125277177 19.540361543092942 19 23.024125631262145 27.46333576611251 27.735444809442907 21.77709379318244 20 22.98254579416196 28.796943237853572 28.7916567545104 19.42885421347407 21 21.575258482833156 27.582221843690025 27.59587837173069 23.246641301746127 22 22.323104168318665 30.236830280443 27.28746508779813 20.15260046344021 23 20.872314399707488 29.736869541977672 28.537004051436654 20.853812006878194 24 22.686169587895304 27.855833842394624 29.428980076124244 20.02901649358583 25 21.475519608102275 29.000402353031014 28.997318625421077 20.52675941344564 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 193.0 1 193.0 2 196.0 3 199.0 4 199.0 5 199.0 6 356.5 7 514.0 8 514.0 9 514.0 10 837.0 11 1160.0 12 1160.0 13 1160.0 14 2083.5 15 3007.0 16 3007.0 17 3007.0 18 5547.5 19 8088.0 20 8088.0 21 8088.0 22 13499.0 23 18910.0 24 18910.0 25 18910.0 26 28184.0 27 37458.0 28 37458.0 29 37458.0 30 50096.5 31 62735.0 32 62735.0 33 62735.0 34 70421.5 35 78108.0 36 78108.0 37 78108.0 38 82188.0 39 86268.0 40 86268.0 41 86268.0 42 88598.0 43 90928.0 44 90928.0 45 90928.0 46 91467.0 47 92006.0 48 92006.0 49 92006.0 50 84836.5 51 77667.0 52 77667.0 53 77667.0 54 65163.5 55 52660.0 56 52660.0 57 52660.0 58 43420.0 59 34180.0 60 34180.0 61 34180.0 62 27092.5 63 20005.0 64 20005.0 65 20005.0 66 15083.5 67 10162.0 68 10162.0 69 10162.0 70 7254.5 71 4347.0 72 4347.0 73 4347.0 74 2987.5 75 1628.0 76 1628.0 77 1628.0 78 1083.0 79 538.0 80 538.0 81 538.0 82 352.0 83 166.0 84 166.0 85 166.0 86 110.0 87 54.0 88 54.0 89 54.0 90 33.5 91 13.0 92 13.0 93 13.0 94 6.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0863443730781769 2 0.032305717818365505 3 0.03142465278695554 4 0.03406784788118544 5 0.024522976707577457 6 0.3913397181179276 7 0.22481842718144357 8 0.22290945294672201 9 0.22848953147898513 10 0.016006014737281093 11 0.0026431950942299052 12 0.0033774159537382125 13 0.0 14 0.0753310601855523 15 0.0019089742347215982 16 0.002936883438033228 17 0.004405325157049842 18 0.0035242601256398735 19 0.0024963509223282438 20 0.0017621300628199367 21 1.468441719016614E-4 22 0.0 23 1.468441719016614E-4 24 2.936883438033228E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 680994.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.27823932511647 #Duplication Level Percentage of deduplicated Percentage of total 1 92.94137972939942 75.54111704852656 2 5.232093312643602 8.505106648727761 3 0.8270677223446451 2.016678248244211 4 0.32636287340324616 1.0610479892520694 5 0.16248083379378542 0.6603078047417882 6 0.10223359403247849 0.49856199137031515 7 0.06942166042198046 0.3949729231087286 8 0.04891274901469395 0.31804336963725205 9 0.03981471640394443 0.2912463043687293 >10 0.20433581062818304 3.1593718638809847 >50 0.023946472713696 1.3404542800617611 >100 0.01886656711545186 3.3459985300743713 >500 0.0016326836919972883 0.9185903511223413 >1k 0.0014512743928864781 1.9485026468831042 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 2128 0.3124843978067354 No Hit CTGTAGGACGTGGAATATGGCAAGA 2066 0.3033800591488325 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1898 0.2787102382693533 No Hit CTTTAGGACGTGAAATATGGCGAGG 1825 0.26799061372053207 No Hit GTCCTACAGTGTGCATTTCTCATTT 1514 0.22232207625911535 No Hit GTATCAACGCAGAGTACTTTTTTTT 1434 0.21057454250698243 No Hit CTGTAGGACCTGGAATATGGCGAGA 1297 0.19045689095645485 No Hit CTGAAGGACCTGGAATATGGCGAGA 1053 0.15462691301244946 No Hit CCCATGTACTCTGCGTTGATACCAC 891 0.1308381571643803 No Hit GTACATGGGAAGCAGTGGTATCAAC 864 0.12687336452303544 No Hit GGTATCAACGCAGAGTACTTTTTTT 818 0.12011853261555902 No Hit TATCAACGCAGAGTACTTTTTTTTT 685 0.10058825775263805 No Hit GTCCTTCAGTGTGCATTTCTCATTT 682 0.10014772523693306 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.468441719016614E-4 0.0 4 0.0 0.0 0.0 1.468441719016614E-4 0.0 5 0.0 0.0 0.0 1.468441719016614E-4 0.0 6 1.468441719016614E-4 0.0 0.0 1.468441719016614E-4 0.0 7 1.468441719016614E-4 0.0 0.0 1.468441719016614E-4 0.0 8 2.936883438033228E-4 0.0 0.0 1.468441719016614E-4 0.0 9 2.936883438033228E-4 0.0 0.0 2.936883438033228E-4 0.0 10 2.936883438033228E-4 0.0 0.0 2.936883438033228E-4 0.0 11 2.936883438033228E-4 0.0 0.0 2.936883438033228E-4 0.0 12 2.936883438033228E-4 0.0 0.0 4.405325157049842E-4 0.0 13 5.873766876066456E-4 0.0 0.0 4.405325157049842E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATGGCG 40 2.817923E-4 16.578419 16 CTGTGCG 35 0.0021676093 16.286724 9 GTCGAGG 65 1.9705294E-7 16.031878 19 TATGTCG 90 1.4006218E-10 15.788972 16 TGTCGAG 90 1.4006218E-10 15.788972 18 ATTGGAC 45 6.5512303E-4 14.837696 3 TAGGACC 415 0.0 14.707829 4 ATGTCGA 100 6.85759E-10 14.210073 17 TTGGACA 60 3.9544943E-4 12.716145 4 GGACCTG 765 0.0 12.590545 6 AGGACCT 785 0.0 12.149182 5 CCTGGTA 55 0.003063965 12.091659 9 ATAGGAC 95 9.874202E-7 12.0486555 3 TATACTG 95 9.891864E-7 12.046873 5 GGCGAGG 580 0.0 11.923396 19 CTGTAGG 1205 0.0 11.553627 1 GTAGACA 75 1.9968793E-4 11.4445305 4 TGTAGGA 1230 0.0 11.401379 2 TCCAACG 75 2.125212E-4 11.368059 18 ACTGTTC 260 0.0 11.327625 8 >>END_MODULE