FastQCFastQC Report
Thu 26 May 2016
SRR1926714_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926714_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715971
Sequences flagged as poor quality0
Sequence length25
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG704.5136658E-617.1126921
TGTTAGG550.003974972814.8680892
GTCCTAA1800.014.7887451
CTTATAC601.2385985E-414.5816293
TGTAGGA3700.014.3657882
GTATAAC751.553424E-414.1971971
TGTAGAA2400.014.1969592
TTACACA1302.5465852E-1114.0035074
TATAAGG700.001244293813.6290822
TCCTAGA1006.9203743E-613.6290812
GTGTTAG700.001487899513.3098711
ATTACAC500.00909355613.1234663
CTGTAGG3350.013.1112171
GTAGGAC4300.012.9708673
TAGGACC2000.012.8502764
TCCAACG607.039292E-512.79775218
AGGACCT2950.012.6984985
CTCCTAG958.073736E-512.6093521
CTAATAC704.570274E-412.4985393
GTGTAGG750.002499664712.4225471
Produced by FastQC (version 0.11.5)