FastQCFastQC Report
Thu 26 May 2016
SRR1926714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715971
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10350.14455892766606468No Hit
CTGTAGGACGTGGAATATGGCAAGA10200.1424638707433681No Hit
CTTTAGGACGTGAAATATGGCGAGG10110.14120683658975014No Hit
GTCCTAAAGTGTGTATTTCTCATTT9490.13254726797593758No Hit
GTATCAACGCAGAGTACTTTTTTTT8100.11313307382561585No Hit
GTCCTACAGTGTGCATTTCTCATTT7490.10461317567331638No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG508.91474E-515.15731618
TTCCTCG456.854237E-414.74966913
GTCCTAG653.2460957E-614.6665261
TAGGACC2800.013.9613524
AATCCCG1301.8189894E-1213.84562519
TCCAACG852.7858914E-713.37410318
GTGTAAT957.035851E-813.0454881
GTCCTAT905.167567E-712.710991
GTCTAAG751.4238494E-512.710991
TATGTCG604.1828785E-412.63197716
CCAACGA604.1853436E-412.63109519
GTAGGAC6550.012.2301353
TTAGGAC4400.011.9207423
TAGGACA2800.011.9182284
TGTAGGA7300.011.89226152
AGGACCT4350.011.8360335
GATATAC2500.011.821221
TGTACTG1052.5867848E-711.8047215
CTGTAGG7200.011.7841461
ATGTCGA658.200628E-411.66028717
Produced by FastQC (version 0.11.5)