FastQCFastQC Report
Thu 26 May 2016
SRR1926713_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926713_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674040
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9300.13797400747730104No Hit
GTCCTAAAGTGTGTATTTCTCATTT8140.12076434632959468No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAT552.3372451E-416.9865281
GTCTAGG500.002421825716.015871
AATACTA400.00285856115.6239395
TAAGGGA601.478949E-414.2804514
CGTGCAA400.006544518313.7932079
ACCATCC500.001238462413.6388958
TGTAGGA5950.013.5556322
CTGTAGG5750.013.4626141
CAATACT652.9152908E-413.1819544
ATTGAAC500.00902561513.1381373
GTAGGAC6450.013.0702413
AATGTGC450.00973208112.9894248
GTCCTAC6700.012.748951
GTCCTAA3150.012.7110061
TAGGACC2950.012.707184
CACTGTT2350.012.5648767
TCCTACA7350.012.4615542
GTCTATA750.00244807712.4567861
TAGGACA2150.012.4538824
CTATAGG1102.288934E-512.1332341
Produced by FastQC (version 0.11.5)