##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926713_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 674040 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.72674767076138 27.0 10.0 31.0 2.0 31.0 2 16.737402824758174 22.0 2.0 31.0 2.0 31.0 3 20.45821612960655 25.0 15.0 31.0 2.0 31.0 4 22.469390540620736 28.0 7.0 35.0 2.0 37.0 5 23.062904278677824 30.0 7.0 35.0 2.0 37.0 6 23.999683995015133 31.0 7.0 35.0 2.0 37.0 7 23.65047623286452 31.0 8.0 35.0 2.0 37.0 8 23.950342709631474 31.0 12.0 35.0 2.0 37.0 9 26.772679662928017 32.0 17.0 37.0 2.0 39.0 10 27.554820188712835 32.0 25.0 38.0 2.0 39.0 11 24.56961456293395 30.0 8.0 37.0 2.0 39.0 12 25.77602664530295 31.0 17.0 37.0 2.0 39.0 13 22.328192688861193 28.0 2.0 37.0 2.0 39.0 14 25.771992760073587 31.0 17.0 38.0 2.0 40.0 15 26.928372203430065 31.0 17.0 38.0 2.0 40.0 16 27.278608094475107 32.0 20.0 39.0 2.0 40.0 17 26.601379740074773 31.0 17.0 38.0 2.0 40.0 18 26.705762269301527 31.0 17.0 38.0 2.0 40.0 19 27.11033766542045 31.0 20.0 39.0 2.0 40.0 20 27.39377781734022 31.0 24.0 39.0 2.0 40.0 21 25.173911043854964 30.0 10.0 38.0 2.0 40.0 22 26.144405376535516 31.0 15.0 38.0 2.0 40.0 23 26.141711174411014 31.0 9.0 39.0 2.0 40.0 24 25.147219749569757 31.0 2.0 39.0 2.0 40.0 25 24.95572221233161 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.951334536494706 1101 2 10.79773333360474 1101 3 7.343104281923534 1101 4 8.626323248639764 1101 5 8.320404166369318 1101 6 8.031967092190925 1101 7 7.364722255987232 1101 8 5.974288440231533 1101 9 5.873134028430091 1101 10 5.446426115670171 1101 11 8.111969608719676 1101 12 5.8141195777154735 1101 13 10.585384535662715 1101 14 7.7229730784901065 1101 15 6.876859035017542 1101 16 6.440451806543347 1101 17 2.36782980354252 1101 18 4.771796455456229 1101 19 5.573525458754638 1101 20 3.7897448506691838 1101 21 0.8482486825074389 1101 22 4.580004472549362 1101 23 5.766555841994414 1101 24 7.205820443355599 1101 25 7.731164148101545 1102 1 5.587046169774318 1102 2 10.660628611748006 1102 3 6.7718856637747535 1102 4 8.104321863396418 1102 5 7.607885496591461 1102 6 6.690738469506854 1102 7 6.548615925484906 1102 8 6.774259001942479 1102 9 5.677987311434414 1102 10 4.987702108596018 1102 11 7.749314544026419 1102 12 5.04571149429005 1102 13 9.567499654316205 1102 14 7.059002182049376 1102 15 4.8162609894509565 1102 16 5.385287142651652 1102 17 6.097791780331104 1102 18 6.2470103873880305 1102 19 4.810759963119203 1102 20 5.210196093316149 1102 21 4.319260560184624 1102 22 5.121916302632158 1102 23 5.650003379451647 1102 24 6.956898788804207 1102 25 6.4951288430752925 1103 1 4.691340648301924 1103 2 9.235562879933806 1103 3 6.176793639234877 1103 4 7.680132555771529 1103 5 6.846272876083134 1103 6 6.70300840815716 1103 7 6.4749962935830645 1103 8 6.092400982661147 1103 9 5.149503525281915 1103 10 4.1997967624084644 1103 11 5.212066515980844 1103 12 3.9992610122567456 1103 13 8.368551363343371 1103 14 6.378897011146659 1103 15 5.441151436427294 1103 16 5.088179342476362 1103 17 2.998755846939339 1103 18 1.834214594224143 1103 19 3.6477450639079834 1103 20 4.2969620880576045 1103 21 6.302608500587777 1103 22 2.0325210353227803 1103 23 4.004078751931928 1103 24 5.440684941302017 1103 25 1.4811060560463716 1104 1 5.754069198575429 1104 2 10.456201158515814 1104 3 7.046178480118556 1104 4 7.331392037774609 1104 5 7.317096261498225 1104 6 7.259676031838747 1104 7 7.136642913305632 1104 8 6.9647777543979785 1104 9 5.738446225272849 1104 10 4.637071234287337 1104 11 7.575001328154073 1104 12 4.954911681663496 1104 13 9.187531991346635 1104 14 6.686047423670459 1104 15 6.018592580009756 1104 16 5.5884513361902854 1104 17 6.011674792812595 1104 18 3.814303143288786 1104 19 4.411399903885016 1104 20 3.591349195555562 1104 21 2.9750676259768802 1104 22 4.8572452639183865 1104 23 5.3506123431556 1104 24 6.366993533281224 1104 25 5.3024704067369335 1105 1 3.316174385477776 1105 2 9.429860967266901 1105 3 6.082624437811976 1105 4 5.362201947945575 1105 5 1.678143783955484 1105 6 4.650685884055498 1105 7 5.51564175422131 1105 8 2.922029275696154 1105 9 3.652710206727498 1105 10 3.18899096769794 1105 11 6.491156065911245 1105 12 3.721073663697691 1105 13 5.026086033653215 1105 14 3.5883366152091405 1105 15 3.9459614585956686 1105 16 4.1069966853644395 1105 17 4.88213987539735 1105 18 2.5090148210633423 1105 19 2.250714595278815 1105 20 1.0895743476853816 1105 21 2.564288141769403 1105 22 3.8010006971261525 1105 23 0.9543648786525551 1105 24 4.470246213663721 1105 25 5.254230766053073 1106 1 6.785073559123941 1106 2 11.531007515736537 1106 3 7.976893093693629 1106 4 9.461116921588943 1106 5 8.964604724404875 1106 6 7.930077374428684 1106 7 7.762110524757809 1106 8 7.524329363784634 1106 9 6.502975171282376 1106 10 5.793392595237478 1106 11 8.177924616925658 1106 12 5.330806741621039 1106 13 4.897541171448765 1106 14 6.266757734072662 1106 15 6.050869245337655 1106 16 6.073390231135495 1106 17 6.6396239006214195 1106 18 6.661216333948033 1106 19 6.439767853853461 1106 20 5.949288549505873 1106 21 6.476932334221761 1106 22 6.60308266135943 1106 23 7.242003128628085 1106 24 7.8926332716483145 1106 25 7.063895330714214 1107 1 4.209200829334275 1107 2 -14.188626427691084 1107 3 -5.597038879390924 1107 4 1.9810334283354472 1107 5 4.997864951378407 1107 6 5.42759103437011 1107 7 5.391470780774835 1107 8 5.685401312928665 1107 9 3.9937016114459922 1107 10 3.4715947759422825 1107 11 6.007866828519713 1107 12 3.4125071702161236 1107 13 5.008522597185642 1107 14 4.457330542306899 1107 15 4.0155352587519495 1107 16 3.836046543480606 1107 17 4.540775597222783 1107 18 -0.9389144974829975 1107 19 2.0710457946427354 1107 20 2.553790833627229 1107 21 -1.0719313052263608 1107 22 2.1503543374403513 1107 23 -0.4581771038827078 1107 24 3.004608147670602 1107 25 3.7384480745099005 1108 1 5.020318087846505 1108 2 10.608547304644745 1108 3 0.20882574127600506 1108 4 5.801565024847157 1108 5 7.19783335554472 1108 6 7.353773403352491 1108 7 7.293372497391395 1108 8 6.918464657829055 1108 9 5.461607431452801 1108 10 4.29888150745499 1108 11 6.7305191428128275 1108 12 2.3194865949549808 1108 13 3.415761704420273 1108 14 4.695222761188731 1108 15 4.795247207808075 1108 16 4.770051932400911 1108 17 0.26988200466800905 1108 18 3.5174174838741052 1108 19 4.490334034076778 1108 20 4.684122291551205 1108 21 4.740166373361294 1108 22 1.5046812716003295 1108 23 4.332630000162506 1108 24 5.234669888370512 1108 25 5.862281367839923 1109 1 3.7806605917776643 1109 2 -14.188626427691084 1109 3 -5.816547263097572 1109 4 2.4606506738997 1109 5 1.737394628429353 1109 6 3.078656212742029 1109 7 4.2292609171953615 1109 8 4.436321760819119 1109 9 3.752803493818522 1109 10 3.180349874734347 1109 11 1.3806522403881978 1109 12 1.8156246443406197 1109 13 3.2971467229244453 1109 14 3.921465569566177 1109 15 3.9090889835537297 1109 16 3.7527615266635372 1109 17 4.070203641637956 1109 18 4.326526463141839 1109 19 2.757006207716092 1109 20 1.9023554314707312 1109 21 0.9836497270480997 1109 22 3.1322089906494703 1109 23 3.5535064869520987 1109 24 4.804043976278166 1109 25 5.226863794197186 1110 1 3.371025753337694 1110 2 9.416659915921251 1110 3 6.4209430251588095 1110 4 7.699480295407909 1110 5 8.397678822640561 1110 6 4.198514707214716 1110 7 6.346915585339659 1110 8 7.1994845749964895 1110 9 5.386042306087067 1110 10 5.049857957665736 1110 11 3.1306370565453854 1110 12 5.647472064467692 1110 13 10.328296080667617 1110 14 7.526044385541944 1110 15 6.731390696370074 1110 16 6.4005886595990695 1110 17 6.757303685414751 1110 18 6.629790003382965 1110 19 5.74489147307839 1110 20 5.859126045016296 1110 21 3.7122391283314293 1110 22 5.93898611155624 1110 23 6.497847646208918 1110 24 7.450788915325887 1110 25 7.768994028186118 1111 1 5.166151160107226 1111 2 9.622093840315618 1111 3 7.49019309341492 1111 4 8.053685978743825 1111 5 8.53446038094604 1111 6 7.481458796235444 1111 7 7.598069298103184 1111 8 7.6230244476342754 1111 9 6.016478628669137 1111 10 5.204761698100654 1111 11 7.233058857178506 1111 12 6.532603300678112 1111 13 8.63175020887104 1111 14 6.221073126899245 1111 15 5.793839504286307 1111 16 5.5384888802323395 1111 17 6.925550204353055 1111 18 6.646911587810244 1111 19 5.27674530907187 1111 20 4.95595527191437 1111 21 7.916325640185853 1111 22 7.257199055866629 1111 23 7.155576820715815 1111 24 6.7471052990063995 1111 25 7.44943367133579 1112 1 -2.616602269488009 1112 2 7.5143271588068075 1112 3 5.444188810396454 1112 4 -0.7680005127855516 1112 5 3.9757787873239216 1112 6 2.9579191571115544 1112 7 5.599377527011438 1112 8 5.847991052812063 1112 9 4.320706519176529 1112 10 3.088115174342626 1112 11 -0.8289198125414003 1112 12 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3.6346016993048096 2204 4 6.367070954371673 2204 5 1.2298244562243923 2204 6 4.288649304717879 2204 7 5.4489833261441945 2204 8 5.2988458846926925 2204 9 3.7410443639197872 2204 10 3.2503602212817277 2204 11 5.094467978911737 2204 12 4.902981721038273 2204 13 3.6109599832330446 2204 14 4.547555596919473 2204 15 4.613682664725182 2204 16 4.272468756545852 2204 17 -0.9462091112561346 2204 18 2.8563208022793596 2204 19 3.6809172645818293 2204 20 3.9369791919760395 2204 21 5.718415675135692 2204 22 5.137110435139004 2204 23 4.1571330447425225 2204 24 5.832406371844641 2204 25 5.889079999864247 2205 1 5.854358473804258 2205 2 10.575146527233791 2205 3 7.139614379030533 2205 4 7.531871284795507 2205 5 7.786561174283904 2205 6 7.263278299986812 2205 7 7.307659262393191 2205 8 6.93286105415044 2205 9 5.45461253694172 2205 10 5.030109919452265 2205 11 7.363997356016604 2205 12 6.5256920051912175 2205 13 9.674104439680065 2205 14 7.093323918978733 2205 15 5.837224207434769 2205 16 5.556415183982583 2205 17 6.063829245636267 2205 18 5.977543601523777 2205 19 4.732485333624236 2205 20 4.816550680789575 2205 21 -1.1755569782485544 2205 22 3.3138568579544234 2205 23 4.81203943428449 2205 24 6.563017078269997 2205 25 6.760194487100758 2206 1 4.644553855589567 2206 2 9.337256891947373 2206 3 6.565409630771658 2206 4 2.372691303918039 2206 5 6.171351354576174 2206 6 6.484387022686793 2206 7 6.650871655549135 2206 8 6.6970256505813595 2206 9 5.33988489583075 2206 10 4.513645200796276 2206 11 4.383814690577218 2206 12 5.242662085426282 2206 13 8.70714636246839 2206 14 0.9427243851075708 2206 15 3.0930223536192507 2206 16 3.942133369454673 2206 17 5.035685928165211 2206 18 -0.3805669442639861 2206 19 2.228976335472016 2206 20 3.3330805587153947 2206 21 4.10721847742051 2206 22 -1.2668048310012878 2206 23 -2.282761130337729 2206 24 2.166298636276153 2206 25 3.6282963495584575 2207 1 1.3996922561453573 2207 2 -14.188626427691084 2207 3 -6.480234869000878 2207 4 -4.524879236459519 2207 5 1.2133260972241402 2207 6 1.7368293543191378 2207 7 2.8968730450208895 2207 8 3.034753335277067 2207 9 0.5873695640326524 2207 10 0.3351806023500856 2207 11 -1.7444229725711011 2207 12 -2.5216644615953463 2207 13 0.5649110057021183 2207 14 -5.487239537998924 2207 15 -1.830682246096508 2207 16 -0.7172612581563165 2207 17 0.4341144849836738 2207 18 0.5819293087468296 2207 19 -0.006370556378413994 2207 20 -0.2946468085882863 2207 21 -4.289428452164305 2207 22 -4.894819261758691 2207 23 -1.9510377974393407 2207 24 -0.006415523992714611 2207 25 0.42530228478932486 2208 1 4.733794094060965 2208 2 6.644571294033895 2208 3 6.224577655225875 2208 4 8.092601740083747 2208 5 3.556548717938842 2208 6 6.148198379696552 2208 7 6.699392976435174 2208 8 6.672342777826053 2208 9 5.263214750286178 2208 10 4.562949436357986 2208 11 6.77664129336268 2208 12 6.210636128227847 2208 13 8.972660074172595 2208 14 3.5913895857871196 2208 15 4.911846136307275 2208 16 5.271330604448753 2208 17 6.229870831083122 2208 18 0.7623306958265914 2208 19 3.3262121987140034 2208 20 4.726452728004148 2208 21 7.0604187665172695 2208 22 6.305950406871723 2208 23 1.9406617244860875 2208 24 3.7548719598124656 2208 25 4.953542907508517 2209 1 2.765644824540974 2209 2 4.434365964532077 2209 3 5.082316148815906 2209 4 0.8342172629473161 2209 5 5.159104452246471 2209 6 5.444515131730899 2209 7 6.061303332462071 2209 8 4.788276603029452 2209 9 4.380018644895667 2209 10 3.2186165457398097 2209 11 -0.5208522877260684 2209 12 3.1632197529026023 2209 13 2.2889471544328863 2209 14 3.829373858508827 2209 15 4.399772805619687 2209 16 3.7017955516565166 2209 17 1.143588833678887 2209 18 3.193600371259752 2209 19 4.1060474976705414 2209 20 4.277188091407723 2209 21 6.407836448666128 2209 22 5.229592015310228 2209 23 0.2644389674596752 2209 24 -2.9881198546571 2209 25 0.21798691940198722 2210 1 -2.0444579392009743 2210 2 -14.188626427691084 2210 3 -7.748042178610378 2210 4 -2.0766758453242 2210 5 -0.39229371839971705 2210 6 -1.0165146284364965 2210 7 -1.2759247378463705 2210 8 -21.330911900774435 2210 9 -13.321608668804776 2210 10 -7.429006545208566 2210 11 -10.093013207179157 2210 12 -4.770737769395726 2210 13 -5.521722686247301 2210 14 -4.279949174443043 2210 15 -6.01311454848878 2210 16 -5.334062967196136 2210 17 -4.9365585456751155 2210 18 -5.081266672700082 2210 19 -10.417009714175908 2210 20 -7.381145778930289 2210 21 -5.380769701995906 2210 22 -10.162130026015124 2210 23 -8.267502702460607 2210 24 -10.781711430380444 2210 25 -8.36649124472433 2211 1 -0.05948095128379549 2211 2 4.898291921738242 2211 3 0.7592079070205564 2211 4 1.5320376434177945 2211 5 0.8222300751883971 2211 6 -0.24068499265339582 2211 7 -21.129736397039196 2211 8 -13.536355272152838 2211 9 -9.250124359934986 2211 10 -7.742603162491449 2211 11 -10.14304686899406 2211 12 -5.537549188428233 2211 13 -8.021173467162038 2211 14 -5.626952485505772 2211 15 -5.894399760518841 2211 16 -12.112117826111794 2211 17 -8.252941022633504 2211 18 -6.418352543793048 2211 19 -9.492067293596136 2211 20 -7.42412187707318 2211 21 -5.321350889962691 2211 22 -6.635949008129085 2211 23 -8.555643900978701 2211 24 -22.637316811546363 2211 25 -22.47770200705617 2212 1 -1.0542620372665041 2212 2 -14.188626427691084 2212 3 -7.97437274062144 2212 4 -19.979814858777114 2212 5 -20.461352014363843 2212 6 -21.363599830230218 2212 7 -10.15984588609029 2212 8 -9.645692922672245 2212 9 -5.39471511376566 2212 10 -5.697424109281755 2212 11 -5.877503076577867 2212 12 -2.9615997706395483 2212 13 -6.208705109081253 2212 14 -3.9786832584337475 2212 15 -4.003500296909451 2212 16 -4.039663438539137 2212 17 -4.252942624755995 2212 18 -3.3716835166659074 2212 19 -3.8778399652315194 2212 20 -4.161595682370436 2212 21 -1.9985039823795248 2212 22 -3.365030094752644 2212 23 -3.455585743932243 2212 24 -2.1975357896485512 2212 25 -2.7733224450123757 2213 1 -19.215758387631467 2213 2 -14.188626427691084 2213 3 -8.806004205134563 2213 4 -5.74673942357402 2213 5 -20.461352014363843 2213 6 -10.899383195800818 2213 7 -21.129736397039196 2213 8 -10.740002809865343 2213 9 -8.451003851429611 2213 10 -7.2881655428753405 2213 11 -6.700764029012603 2213 12 -5.3124747653451045 2213 13 -19.683157663825778 2213 14 -12.67182625071092 2213 15 -7.155865500427783 2213 16 -6.481848136897153 2213 17 -5.045757008694778 2213 18 -5.055105914271408 2213 19 -5.897395867474952 2213 20 -5.9286984933659355 2213 21 -4.044975060610195 2213 22 -4.884040669517219 2213 23 -4.580098655310351 2213 24 -3.6489222274070983 2213 25 -4.5067155467080084 2214 1 -19.215758387631467 2214 2 -14.188626427691084 2214 3 -12.69310595797328 2214 4 -19.979814858777114 2214 5 -20.461352014363843 2214 6 -21.363599830230218 2214 7 -16.77325971310137 2214 8 -15.343347133935056 2214 9 -14.267597190842928 2214 10 -14.312772431962442 2214 11 -14.840498046510927 2214 12 -12.75261467174011 2214 13 -19.683157663825778 2214 14 -17.288778375865007 2214 15 -24.462441902748868 2214 16 -18.880925115480252 2214 17 -24.15987693132534 2214 18 -18.419992014831635 2214 19 -15.636889358219683 2214 20 -14.32136743835546 2214 21 -11.815212881437805 2214 22 -12.870162275835055 2214 23 -12.72247145173452 2214 24 -11.527472251960871 2214 25 -11.317080245398138 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 111978.0 3 3.0 4 460.0 5 1939.0 6 2890.0 7 2716.0 8 2319.0 9 2127.0 10 1953.0 11 1920.0 12 1793.0 13 2017.0 14 3105.0 15 6986.0 16 10419.0 17 10797.0 18 11055.0 19 12727.0 20 15237.0 21 17571.0 22 18326.0 23 20014.0 24 20931.0 25 21785.0 26 17776.0 27 14066.0 28 14550.0 29 17519.0 30 17152.0 31 19446.0 32 25424.0 33 32124.0 34 39743.0 35 45841.0 36 44914.0 37 47325.0 38 37092.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.99470888605966 19.653613426971674 12.960320067901488 25.39135761906718 2 12.817217967468657 20.552873944553735 43.08790751790813 23.542000570069483 3 18.093171013322312 25.954849883433706 30.196033275812574 25.755945827431407 4 10.826275641224505 18.63230716730608 39.61476548469713 30.926651706772283 5 11.43183144023884 39.87749219312995 36.52276860780344 12.167907758827768 6 30.01851780946845 38.751474985293655 19.17010974934822 12.059897455889674 7 25.72018451679132 32.94539765729925 23.45668889767124 17.877728928238188 8 22.977079924037415 39.3444224185692 21.24342292776189 16.43507472963149 9 26.371928803040856 15.451308688993823 20.736270314349756 37.44049219361557 10 13.820474386303205 30.3735283705305 35.911600545340875 19.894396697825414 11 32.15405828379589 22.55199853596355 26.246324132621506 19.047619047619047 12 23.033252741641107 25.24248432559884 33.45085850641634 18.273404426343713 13 27.96329118630803 22.513098275578155 25.680410929070113 23.843199609043698 14 19.39925415914892 23.77835371924764 28.603388869109757 28.21900325249368 15 21.69662639393828 33.229744286536175 23.937026428212942 21.1366028913126 16 20.115115007824393 28.80018864284336 30.25773022235969 20.826966126972557 17 20.374208924902746 29.244193428473963 29.883019554871588 20.49857809175171 18 20.043142433341778 28.032783425587592 32.51419992040809 19.409874220662545 19 22.32524083152791 27.22006442262204 28.038002717923533 22.416692027926512 20 22.392137314276788 28.66666170660754 29.32049670032067 19.620704278795003 21 21.146547540534826 27.0548946654555 27.318386570934383 24.480171223075295 22 21.754540022813277 29.67128967427713 28.390155287187753 20.184015015721847 23 20.798606108228626 28.966776239076797 29.243950370085397 20.99066728260918 24 21.807746317886416 28.13608308023905 29.639463113549525 20.416707488325006 25 21.54861498972086 28.29794060676641 29.342788558662402 20.810655844850327 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 760.0 1 760.0 2 1595.5 3 2431.0 4 2431.0 5 2431.0 6 3486.0 7 4541.0 8 4541.0 9 4541.0 10 5423.0 11 6305.0 12 6305.0 13 6305.0 14 7978.5 15 9652.0 16 9652.0 17 9652.0 18 13842.0 19 18032.0 20 18032.0 21 18032.0 22 25162.5 23 32293.0 24 32293.0 25 32293.0 26 41695.0 27 51097.0 28 51097.0 29 51097.0 30 61322.5 31 71548.0 32 71548.0 33 71548.0 34 76380.0 35 81212.0 36 81212.0 37 81212.0 38 82573.0 39 83934.0 40 83934.0 41 83934.0 42 82957.5 43 81981.0 44 81981.0 45 81981.0 46 79539.5 47 77098.0 48 77098.0 49 77098.0 50 68863.5 51 60629.0 52 60629.0 53 60629.0 54 50668.5 55 40708.0 56 40708.0 57 40708.0 58 33119.0 59 25530.0 60 25530.0 61 25530.0 62 19898.5 63 14267.0 64 14267.0 65 14267.0 66 10748.5 67 7230.0 68 7230.0 69 7230.0 70 5165.5 71 3101.0 72 3101.0 73 3101.0 74 2118.0 75 1135.0 76 1135.0 77 1135.0 78 767.5 79 400.0 80 400.0 81 400.0 82 252.5 83 105.0 84 105.0 85 105.0 86 71.5 87 38.0 88 38.0 89 38.0 90 23.5 91 9.0 92 9.0 93 9.0 94 6.0 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.302474630585722 2 36.49961426621566 3 5.94415761675865 4 13.9193519672423 5 13.533321464601508 6 14.755504124384307 7 9.5292564239511 8 7.347041718592369 9 0.1200225505904694 10 1.5184558779894368 11 5.960477123019405 12 5.869978042846122 13 16.211352441991572 14 1.6562815263189128 15 3.040769093822325 16 3.1087175835262 17 3.0177734259094415 18 1.580915079223785 19 1.5278025043024153 20 0.29746009138923507 21 4.61931042668091 22 1.6717108776927183 23 0.12120942377306985 24 9.141890688979883 25 0.12343481099044568 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 674040.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.0257620190683 #Duplication Level Percentage of deduplicated Percentage of total 1 96.63977812932433 87.96709445575436 2 2.4164954560194403 4.399266805995709 3 0.4192541036832877 1.144887728021782 4 0.16299508588977304 0.5934700759392031 5 0.09269758965951609 0.4218934368044178 6 0.04908636647023017 0.2680874347619967 7 0.03711059096905722 0.2364613875355471 8 0.030338231081301303 0.2209248481988826 9 0.01908057257647331 0.15631412926802582 >10 0.11495598493757359 1.9763276856076177 >50 0.008598301466795721 0.5426458419405165 >100 0.008429463090656432 1.333717906745827 >500 0.0011801248316271886 0.7389082634261386 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 930 0.13797400747730104 No Hit GTCCTAAAGTGTGTATTTCTCATTT 814 0.12076434632959468 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.9671829565010977E-4 0.0 10 0.0 0.0 0.0 2.9671829565010977E-4 0.0 11 0.0 0.0 0.0 2.9671829565010977E-4 0.0 12 0.0 0.0 0.0 2.9671829565010977E-4 1.4835914782505488E-4 13 0.0 0.0 0.0 2.9671829565010977E-4 1.4835914782505488E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTCTAT 55 2.3372451E-4 16.986528 1 GTCTAGG 50 0.0024218257 16.01587 1 AATACTA 40 0.002858561 15.623939 5 TAAGGGA 60 1.478949E-4 14.280451 4 CGTGCAA 40 0.0065445183 13.793207 9 ACCATCC 50 0.0012384624 13.638895 8 TGTAGGA 595 0.0 13.555632 2 CTGTAGG 575 0.0 13.462614 1 CAATACT 65 2.9152908E-4 13.181954 4 ATTGAAC 50 0.009025615 13.138137 3 GTAGGAC 645 0.0 13.070241 3 AATGTGC 45 0.009732081 12.989424 8 GTCCTAC 670 0.0 12.74895 1 GTCCTAA 315 0.0 12.711006 1 TAGGACC 295 0.0 12.70718 4 CACTGTT 235 0.0 12.564876 7 TCCTACA 735 0.0 12.461554 2 GTCTATA 75 0.002448077 12.456786 1 TAGGACA 215 0.0 12.453882 4 CTATAGG 110 2.288934E-5 12.133234 1 >>END_MODULE