FastQCFastQC Report
Thu 26 May 2016
SRR1926713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926713_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674040
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGGACGTGAAATATGGCGAGG17700.2625956916503472No Hit
GTCCTACAGTGGACATTTCTAAATT17350.2574031214764702No Hit
GTCCTAAAGTGTGTATTTCTCATTT16950.25146875556346804No Hit
CTGTAGGACGTGGAATATGGCAAGA16870.2502818823808676No Hit
GTACATGGGAAGCAGTGGTATCAAC16550.24553438965046584No Hit
CCCATGTACTCTGCGTTGATACCAC16380.24301228413743992No Hit
GTATCAACGCAGAGTACTTTTTTTT14430.2140822503115542No Hit
GTCCTACAGTGTGCATTTCTCATTT12730.18886119518129488No Hit
CTGTAGGACCTGGAATATGGCGAGA11780.17476707613791465No Hit
GGTATCAACGCAGAGTACTTTTTTT8940.13263307815559908No Hit
CTGAAGGACCTGGAATATGGCGAGA8740.12966589519909796No Hit
TATCAACGCAGAGTACTTTTTTTTT7860.11661029019049314No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAA402.6803752E-416.6874711
TTGGACC500.001449697813.3579594
CGAAATC500.001523118513.2706913
TAGGACC4150.013.1050554
CTAGGAC1259.458745E-1112.9772723
GGCGAGG4950.012.82168219
ACGAAAT604.1634357E-412.63875312
CCAACGT604.186962E-412.63031519
TGCACCT959.837731E-712.0522945
TGGCGAG11700.011.90164318
GTTATAG657.764741E-411.7362441
TAGAGTG907.090992E-611.661715
TAAGACA1156.633309E-811.6156164
CTAAGAC751.9871106E-411.4505343
AGTCACC752.0714568E-411.399428
ATAGGAC1354.3892214E-911.3091693
GGCGAGA6800.011.28369919
TGTAGGA11500.011.2016112
TTATACT600.005682773411.1316324
GTCCTAT1201.2060264E-711.1249821
Produced by FastQC (version 0.11.5)