FastQCFastQC Report
Thu 26 May 2016
SRR1926712_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926712_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813724
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11980.14722436600124858No Hit
GTCCTAAAGTGTGTATTTCTCATTT10370.12743878759874355No Hit
CTTTAGGACGTGAAATATGGCGAGG8480.10421223903928113No Hit
CTGTAGGACGTGGAATATGGCAAGA8410.10335199650004177No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAC552.4169128E-416.9134221
TGTAGGA8100.015.4544552
GTCTTAT953.1338823E-715.3873981
TGTAGAA2850.015.2776312
ACTGATC652.5851223E-614.9910248
TAGACTG1053.5106495E-1014.8562185
GACGTGC551.0935387E-414.7975787
TAATACC651.8025887E-514.7911944
GTAGACC450.004594617514.5489923
GTCTTAA550.00470300814.4972191
CGGTTTC501.3216348E-414.47231713
GATATAC1800.014.0274011
TCATATA700.001260340813.606642
TAGGACC3300.013.5959474
CTGTAGG9100.013.4351511
ATTTAGA2100.013.2891181
CCGGTTT552.9660005E-413.156651512
TCCAACG651.01355945E-513.13773518
TGGACAG1058.3433406E-812.8753895
CGTGCAA500.001914655312.8734059
Produced by FastQC (version 0.11.5)