FastQCFastQC Report
Thu 26 May 2016
SRR1926712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926712_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813724
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT24450.3004704297771726No Hit
CTGTAGGACGTGGAATATGGCAAGA23820.29272824692401844No Hit
CTTTAGGACGTGAAATATGGCGAGG23460.2883041424365018No Hit
GTCCTAAAGTGTGTATTTCTCATTT22340.2745402618086722No Hit
GTCCTACAGTGTGCATTTCTCATTT17680.21727268705359556No Hit
CTGTAGGACCTGGAATATGGCGAGA16170.1987160265642896No Hit
GTATCAACGCAGAGTACTTTTTTTT15770.19380035491149333No Hit
CTGAAGGACCTGGAATATGGCGAGA12680.15582679139364208No Hit
GGTATCAACGCAGAGTACTTTTTTT9820.12067973907614866No Hit
GTACATGGGAAGCAGTGGTATCAAC8490.10433513083060103No Hit
CCCATGTACTCTGCGTTGATACCAC8160.1002797017170441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCGGC250.006023667819.0032639
AGTATGC508.693015E-515.2026118
TAGGACC6050.014.8130724
TCCAACG902.3646862E-914.74035118
CCAACGT500.001528090613.26550119
TGTAGGA15650.012.6752342
CTGTAGG14850.012.6445991
AGGACCT9650.012.349675
GGACCTG9150.012.2943826
GTAGGAC15450.012.2822473
GCATAAG701.0564038E-412.2548981
AGGACCG550.002985425812.1341125
GCCTTAG550.002990962212.1311121
CTTAGAC959.917312E-712.0451143
ATTTAGA3000.011.7556261
CCGGTTT658.142221E-411.67058612
GTGTAGA1406.4028427E-1011.5740711
GGCGAGG5850.011.50000819
TAGGACA4350.011.39694
GTTACAA2250.011.37182615
Produced by FastQC (version 0.11.5)