FastQCFastQC Report
Thu 26 May 2016
SRR1926711_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926711_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828166
Sequences flagged as poor quality0
Sequence length25
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT12410.1498491848252645No Hit
GTCCTAAAGTGTGTATTTCTCATTT10370.12521644211426214No Hit
CTTTAGGACGTGAAATATGGCGAGG8750.1056551464319955No Hit
CTGTAGGACGTGGAATATGGCAAGA8670.10468915652176014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGA1107.410017E-814.9024172
GCAGGAC753.4570567E-614.5766513
ATAAAGC450.004537116314.5766513
GTATTAA658.3498313E-414.3686171
TATAGAA1252.2922904E-814.206972
TAGGACC2800.014.1408074
GTCCAGG852.7785698E-514.1271271
GTCCTAC7600.013.69341
GTCCTAA4650.013.4857531
GTATAGA904.7572124E-513.3422871
GTTCAAG700.001461451213.3422861
AAGACTA653.7077782E-412.81314855
TAGAAAT2050.012.5281774
GGCGAGG3950.012.50219619
TCCTACA8750.012.3335242
GGCCATT605.361109E-412.2651711
CCCTGTA905.827003E-412.1427112
GTGTATA4000.012.0080591
GTATAAA907.1043085E-411.8598111
CTAGAGT1452.224624E-911.80816654
Produced by FastQC (version 0.11.5)