##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926711_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 828166 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.339415044809858 27.0 7.0 31.0 2.0 31.0 2 16.4821038294255 16.0 2.0 30.0 2.0 31.0 3 20.100262507758107 25.0 15.0 31.0 2.0 31.0 4 22.11846296515433 28.0 2.0 35.0 2.0 37.0 5 22.676168787417016 29.0 2.0 35.0 2.0 37.0 6 23.59974570315613 31.0 2.0 35.0 2.0 37.0 7 23.230867966084094 30.0 7.0 35.0 2.0 37.0 8 23.502831557924377 31.0 10.0 35.0 2.0 37.0 9 26.251482190768517 32.0 17.0 37.0 2.0 39.0 10 27.00102274181746 32.0 23.0 37.0 2.0 39.0 11 24.06963821262887 30.0 7.0 37.0 2.0 39.0 12 25.24043971860714 31.0 11.0 37.0 2.0 39.0 13 21.881747137651146 27.0 2.0 37.0 2.0 39.0 14 25.23866471214708 31.0 17.0 38.0 2.0 40.0 15 26.356696604303966 31.0 17.0 38.0 2.0 40.0 16 26.71906719184318 31.0 17.0 39.0 2.0 40.0 17 26.04979315741047 31.0 12.0 38.0 2.0 40.0 18 26.12811924179452 31.0 17.0 38.0 2.0 40.0 19 26.51204347920586 31.0 18.0 39.0 2.0 40.0 20 26.77579253434698 31.0 18.0 39.0 2.0 40.0 21 24.614995061376582 30.0 2.0 38.0 2.0 40.0 22 25.550027409963704 31.0 2.0 38.0 2.0 40.0 23 25.547268301282593 31.0 2.0 38.0 2.0 40.0 24 24.599682913811964 31.0 2.0 39.0 2.0 40.0 25 24.40344568600981 31.0 2.0 38.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.51624017563196 1101 2 10.14258295000008 1101 3 6.8677399710058715 1101 4 8.307647274316821 1101 5 7.979146981231658 1101 6 7.482893944207039 1101 7 7.099637258439568 1101 8 5.779698739500688 1101 9 5.484021998082355 1101 10 5.121306096807626 1101 11 7.654182699852871 1101 12 5.320560036663256 1101 13 9.976222884237 1101 14 7.5338729580210675 1101 15 6.738902749170126 1101 16 6.087889807255994 1101 17 2.6143073613215897 1101 18 4.667607506370171 1101 19 5.370943320708172 1101 20 2.9794921951015283 1101 21 0.2865810733696321 1101 22 4.262773291344718 1101 23 5.483027249683001 1101 24 6.8133967239336855 1101 25 7.334058748056233 1102 1 5.9843616386555105 1102 2 11.010005999195403 1102 3 7.226991693835327 1102 4 8.535608066570688 1102 5 8.168958565320981 1102 6 7.389139029652359 1102 7 7.153939150005073 1102 8 7.426581823717701 1102 9 6.6272444454356005 1102 10 5.828260369025504 1102 11 8.400878075133676 1102 12 5.707087026976744 1102 13 10.3539790626889 1102 14 7.879289623285008 1102 15 5.787810954546689 1102 16 6.379397985796384 1102 17 7.392604495477933 1102 18 7.081756518313227 1102 19 6.063956729657313 1102 20 6.193670351706476 1102 21 4.9053155121160685 1102 22 6.173877263242723 1102 23 6.6777953500329765 1102 24 8.153548112657589 1102 25 7.812623714922214 1103 1 4.8431914922577555 1103 2 9.103738044695344 1103 3 6.1861961106731655 1103 4 7.6372130909949725 1103 5 6.8215498080867825 1103 6 6.519853954727381 1103 7 6.432237414783554 1103 8 6.0658944422328105 1103 9 5.01439340028335 1103 10 4.175108884211465 1103 11 5.204733191411027 1103 12 4.198147566160401 1103 13 8.215550946034273 1103 14 6.457522542930224 1103 15 5.546090676102445 1103 16 5.1878632628720815 1103 17 2.881868906545673 1103 18 1.7226966005248059 1103 19 3.5724814338164705 1103 20 4.018244560352251 1103 21 6.298802205134493 1103 22 1.7639916542137506 1103 23 4.131935642335321 1103 24 5.577971904204144 1103 25 1.6687352109805467 1104 1 6.086018929038861 1104 2 10.830926167250563 1104 3 7.333840640427368 1104 4 7.701959318704162 1104 5 7.6897093954977755 1104 6 7.407732213826684 1104 7 7.316383856736564 1104 8 7.369095012257098 1104 9 6.163542483930534 1104 10 4.914650925925997 1104 11 8.058728450902947 1104 12 4.95886775500518 1104 13 9.530891676111295 1104 14 7.18987529877467 1104 15 6.571603625251047 1104 16 6.089104616776698 1104 17 6.384346282495105 1104 18 3.9978418937043863 1104 19 4.7494444064737245 1104 20 3.631549004699753 1104 21 3.063424345924677 1104 22 4.933849196614155 1104 23 5.451945142740058 1104 24 6.7465982717050075 1104 25 5.651293728141866 1105 1 3.454269929774327 1105 2 9.335729136052322 1105 3 6.3900184391649155 1105 4 5.547035737652546 1105 5 1.597837295010649 1105 6 4.657831866306207 1105 7 5.726050053679806 1105 8 2.923637200344835 1105 9 3.603600347867939 1105 10 3.1941429134923602 1105 11 6.468294527514651 1105 12 3.9926307636304976 1105 13 5.0370888439223265 1105 14 3.62437882357176 1105 15 4.07218750456768 1105 16 4.371125812344456 1105 17 5.074232444742442 1105 18 2.7253180940723034 1105 19 2.714231056597143 1105 20 1.6200576234696022 1105 21 2.5551995074739757 1105 22 3.99298475580024 1105 23 1.3703040301505318 1105 24 4.686727249148923 1105 25 5.384431873613899 1106 1 6.592072687298394 1106 2 11.143270738530383 1106 3 7.868328046431646 1106 4 9.143807976593898 1106 5 8.599604585538522 1106 6 7.8167236787475645 1106 7 7.667660610784864 1106 8 7.550693913386429 1106 9 6.518143358669988 1106 10 5.930514578821704 1106 11 8.299632582191407 1106 12 5.743348237681165 1106 13 5.008884723243469 1106 14 6.21910651393879 1106 15 6.288962901680161 1106 16 6.319286820315167 1106 17 6.671227775423155 1106 18 6.655096526334127 1106 19 6.377856453434198 1106 20 5.984535359281242 1106 21 5.881592820964691 1106 22 6.381981467262751 1106 23 6.756520816564521 1106 24 7.435899606338079 1106 25 6.47109980110082 1107 1 3.657371909082343 1107 2 -13.913929799827624 1107 3 -5.727507121329998 1107 4 1.4638619917278177 1107 5 4.280945782031125 1107 6 4.6817813388270295 1107 7 4.414363347318517 1107 8 5.035336722173394 1107 9 3.4966843582548286 1107 10 2.9396022843710767 1107 11 5.592641370410657 1107 12 2.815909105581703 1107 13 4.498384769421744 1107 14 3.7536855249704644 1107 15 3.6422903827762063 1107 16 3.3217871722564 1107 17 4.058567126578019 1107 18 -0.8253888925387116 1107 19 1.8536433447215686 1107 20 2.086462921256526 1107 21 -1.4482743021645277 1107 22 1.4097642016085388 1107 23 -1.2657007979177024 1107 24 2.1294139346055516 1107 25 2.9799548219348466 1108 1 5.423014813812525 1108 2 10.950736866839044 1108 3 0.33975559241639175 1108 4 6.437879757661641 1108 5 7.698480314564993 1108 6 7.738160197365779 1108 7 7.933714446585672 1108 8 7.480617646001953 1108 9 6.2754793837933605 1108 10 5.253224314129014 1108 11 7.567378656664264 1108 12 2.9069390855950274 1108 13 3.7148560108163693 1108 14 5.187979551175218 1108 15 5.241266176691372 1108 16 5.3041553712348595 1108 17 0.5169855143195221 1108 18 3.977855611125513 1108 19 5.202931374923654 1108 20 5.608086727607848 1108 21 5.63422269983414 1108 22 2.291440417464635 1108 23 5.116211260709878 1108 24 5.678900054969752 1108 25 6.633192219225542 1109 1 3.3250352368613925 1109 2 -13.913929799827624 1109 3 -5.844043094673323 1109 4 1.5488104651744052 1109 5 1.1082498258706082 1109 6 2.368052246964613 1109 7 3.6882250228074085 1109 8 3.7601945971325144 1109 9 3.0948097995482833 1109 10 2.3366087050845223 1109 11 0.8842320483491655 1109 12 1.2169638850115128 1109 13 2.674090146483767 1109 14 3.0303705066821465 1109 15 3.0918015523295956 1109 16 3.0539525995353713 1109 17 3.4550555362013604 1109 18 3.5150036782298137 1109 19 1.930350776820081 1109 20 1.1263186750914365 1109 21 0.6880607742323903 1109 22 2.7043390316148894 1109 23 2.952279823802513 1109 24 4.149686342351227 1109 25 4.436039775753624 1110 1 2.973126858630451 1110 2 8.808058995690583 1110 3 5.741167357122276 1110 4 7.213823735252678 1110 5 7.83558955826247 1110 6 3.6127932505870675 1110 7 6.052280272916203 1110 8 6.485799718831085 1110 9 4.947703473429215 1110 10 4.735549244101001 1110 11 2.6449752953197283 1110 12 5.148390065987186 1110 13 9.49390362986399 1110 14 6.986315685628998 1110 15 6.2941457285121025 1110 16 5.906043326416931 1110 17 6.241909884067422 1110 18 6.162897627932235 1110 19 5.107172271282199 1110 20 5.442770200997202 1110 21 3.7179874057164923 1110 22 5.52429195808088 1110 23 5.8276958545474145 1110 24 6.973762800067792 1110 25 7.270217429309575 1111 1 5.039168659966371 1111 2 9.489746170220027 1111 3 7.370253436754947 1111 4 7.996777420470806 1111 5 8.348786191578832 1111 6 7.562778758745402 1111 7 7.506843105537339 1111 8 7.363780795536783 1111 9 6.070653878914758 1111 10 5.4602676542697 1111 11 7.452675230297121 1111 12 6.545275368780867 1111 13 8.445028463732186 1111 14 6.14654683050356 1111 15 5.700042668636009 1111 16 5.394403612668942 1111 17 6.511312267212645 1111 18 6.237739886142528 1111 19 5.194572854388145 1111 20 5.068724803396364 1111 21 7.617659051093501 1111 22 7.101022446055509 1111 23 6.86348831993384 1111 24 6.875726921318744 1111 25 7.232776516933733 1112 1 -2.2160760952783853 1112 2 7.3946101450759585 1112 3 5.456934655777275 1112 4 -0.7735307106579192 1112 5 3.7814885790278012 1112 6 2.5019232827652296 1112 7 5.504794336392834 1112 8 5.643565304404156 1112 9 3.960482138614296 1112 10 2.7620672893356257 1112 11 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5.705539508698983 2204 5 1.1174595676770238 2204 6 3.829261165553884 2204 7 4.934740725977097 2204 8 5.037187493858106 2204 9 3.509540241331397 2204 10 3.0286876612936062 2204 11 4.295840620702993 2204 12 4.62432774396294 2204 13 2.219600132531447 2204 14 3.9004747102346933 2204 15 4.049968804630517 2204 16 3.9299313048448177 2204 17 -1.712168012651432 2204 18 2.1626273953578767 2204 19 3.0889562711892147 2204 20 3.318803186805635 2204 21 5.011616890705511 2204 22 4.430710127008204 2204 23 3.155976129312922 2204 24 4.794424259672379 2204 25 4.869363726832734 2205 1 6.2971957596658505 2205 2 10.77763976836977 2205 3 7.485344808774631 2205 4 8.08354619586132 2205 5 8.08812573380191 2205 6 7.700882153043185 2205 7 7.8907987509036985 2205 8 7.630057901884978 2205 9 6.441226242372295 2205 10 5.716290341088122 2205 11 7.99253789815387 2205 12 7.100274246664252 2205 13 10.42072075836035 2205 14 7.761583846354565 2205 15 6.565165651217303 2205 16 6.210886922525699 2205 17 6.87786693995352 2205 18 6.5922970093434685 2205 19 5.345175834599232 2205 20 5.824845238003096 2205 21 -0.8942570808375514 2205 22 3.931081267361826 2205 23 5.314463488262998 2205 24 7.08666244930377 2205 25 7.5433242731437495 2206 1 4.036298395902076 2206 2 8.740751339928142 2206 3 5.896061336423628 2206 4 2.0688277449976873 2206 5 5.690137483266938 2206 6 6.047686203697758 2206 7 6.180116074808197 2206 8 6.13566061298522 2206 9 4.86316911694578 2206 10 4.018769316390433 2206 11 3.8937676209338257 2206 12 4.785864458729357 2206 13 8.256922948853457 2206 14 0.32146575651216835 2206 15 2.480499102969077 2206 16 3.4085660451380697 2206 17 4.449981443763214 2206 18 -0.7660928284945712 2206 19 1.32933209857811 2206 20 2.9404413182907945 2206 21 3.703780366048523 2206 22 -1.5842068009885537 2206 23 -2.6249692006198373 2206 24 1.6566784357929087 2206 25 3.3708059686601857 2207 1 1.7523019968145803 2207 2 -13.913929799827624 2207 3 -6.11643723372023 2207 4 -3.9178246060578026 2207 5 1.587783033322129 2207 6 2.2615401379643636 2207 7 3.430209306078474 2207 8 3.4504828504799043 2207 9 1.1752906700624948 2207 10 1.006214946982567 2207 11 -1.4803887639434556 2207 12 -1.6982178712247098 2207 13 0.8504671600053122 2207 14 -5.314495660156744 2207 15 -1.3882270835325237 2207 16 -0.16402740235435687 2207 17 0.8470238967732584 2207 18 0.8527493739767777 2207 19 0.4061765200001908 2207 20 0.20592817151920073 2207 21 -3.412420206265928 2207 22 -4.023682269307127 2207 23 -1.154350768076963 2207 24 0.7254561436149416 2207 25 1.3198835603267511 2208 1 5.109627191662689 2208 2 7.146656519390618 2208 3 6.722396200451136 2208 4 8.620274980245789 2208 5 4.205205613153481 2208 6 6.583851836888037 2208 7 7.1251737299732945 2208 8 6.934797884872744 2208 9 5.140628135150582 2208 10 4.780824748439546 2208 11 6.927102869476425 2208 12 6.446183385269297 2208 13 9.135496618851114 2208 14 3.7406592471578435 2208 15 4.953843809698974 2208 16 5.362322580789691 2208 17 6.2844057097592625 2208 18 1.2146960020049882 2208 19 3.8505882696033495 2208 20 4.746518866587216 2208 21 7.287004458791795 2208 22 6.311727062415773 2208 23 2.425820381231052 2208 24 3.6924157987265396 2208 25 4.9981259868694075 2209 1 2.540383234865157 2209 2 4.2250175685934295 2209 3 4.838773136276043 2209 4 0.06598502081953583 2209 5 4.635989086494817 2209 6 4.914897039471043 2209 7 5.339293393954094 2209 8 4.170091330212479 2209 9 3.853619734670552 2209 10 2.9107681737781377 2209 11 -1.024866957370822 2209 12 2.6730443487529243 2209 13 2.225206888009353 2209 14 3.245277796789253 2209 15 3.3512310889720744 2209 16 2.848085195796262 2209 17 1.0976521809861914 2209 18 2.8096099970904227 2209 19 3.4079138310640076 2209 20 3.4851393591867925 2209 21 5.559906910360457 2209 22 4.470177259569898 2209 23 0.19182529579759944 2209 24 -3.226082401170597 2209 25 -0.055088482528844906 2210 1 -2.669309390480592 2210 2 -13.913929799827624 2210 3 -8.063472633589035 2210 4 -2.84924138716935 2210 5 -1.067485143120674 2210 6 -1.713301435992225 2210 7 -1.6833440286178813 2210 8 -20.886049020664714 2210 9 -13.453070825927835 2210 10 -7.735485283699756 2210 11 -10.499964545901735 2210 12 -5.227210433279879 2210 13 -5.927204191502899 2210 14 -4.597790495703638 2210 15 -6.511259853409786 2210 16 -5.928512775816706 2210 17 -5.508318704777441 2210 18 -5.473047423663534 2210 19 -10.683879382539953 2210 20 -7.814827656798766 2210 21 -5.8865974334005 2210 22 -10.40581790970043 2210 23 -8.515728043509217 2210 24 -10.966531970690253 2210 25 -8.722181084500095 2211 1 -0.08299674688111836 2211 2 4.716850546993186 2211 3 0.5511101203547355 2211 4 1.4473286979313897 2211 5 1.0846383107114264 2211 6 0.08170162318466367 2211 7 -20.612285553414328 2211 8 -13.148332257658934 2211 9 -9.129379961100668 2211 10 -7.2730666299943 2211 11 -9.613721536013962 2211 12 -4.776026232324046 2211 13 -6.983043796505402 2211 14 -4.878827770597422 2211 15 -5.250591241420381 2211 16 -12.058545977512733 2211 17 -8.09502604637412 2211 18 -6.148069244365818 2211 19 -8.926667854363437 2211 20 -6.782772617286181 2211 21 -4.340453600743892 2211 22 -5.862507559413977 2211 23 -7.960378334665844 2211 24 -22.099766611696914 2211 25 -21.876141114107792 2212 1 -0.8662944954967848 2212 2 -13.913929799827624 2212 3 -7.704160323499524 2212 4 -19.562786909005027 2212 5 -20.020853018768342 2212 6 -20.886506469300887 2212 7 -9.788711979840754 2212 8 -9.396559531175225 2212 9 -5.0614695651555905 2212 10 -5.40935826845357 2212 11 -5.522297019011411 2212 12 -2.832664518008574 2212 13 -6.173942787982428 2212 14 -3.468885313689647 2212 15 -3.6134425320173342 2212 16 -3.7470278119483247 2212 17 -3.9443898610558534 2212 18 -2.9158681125982113 2212 19 -3.4139875439952654 2212 20 -3.587985344464226 2212 21 -1.4715550779436377 2212 22 -2.832037060012844 2212 23 -2.9333382888396713 2212 24 -1.9053221672524714 2212 25 -2.3798948678615446 2213 1 -18.806985186187475 2213 2 -13.913929799827624 2213 3 -8.574775868842057 2213 4 -5.6563906531547925 2213 5 -20.020853018768342 2213 6 -10.654837202530215 2213 7 -20.612285553414328 2213 8 -10.5319148552981 2213 9 -8.092109799773983 2213 10 -7.182209903239681 2213 11 -6.614692066164647 2213 12 -4.918608496308249 2213 13 -19.208477322516963 2213 14 -12.243516028647976 2213 15 -6.789419210724123 2213 16 -6.067484774370964 2213 17 -4.666518385836483 2213 18 -4.869991502759046 2213 19 -5.696966700999383 2213 20 -5.606656902137342 2213 21 -3.793621293925611 2213 22 -4.5850588025041645 2213 23 -4.374038349274521 2213 24 -3.4429803402460557 2213 25 -4.292677775574251 2214 1 -18.806985186187475 2214 2 -13.913929799827624 2214 3 -12.250858562834749 2214 4 -19.562786909005027 2214 5 -20.020853018768342 2214 6 -20.886506469300887 2214 7 -16.400812950674602 2214 8 -14.814987376829098 2214 9 -13.714689565978329 2214 10 -13.539675229249982 2214 11 -14.556096229180485 2214 12 -12.136595160980315 2214 13 -19.208477322516963 2214 14 -16.607730494486884 2214 15 -23.821400489975293 2214 16 -18.15516882967838 2214 17 -23.569014485680476 2214 18 -17.731527950518323 2214 19 -14.893470451560363 2214 20 -13.437431537232335 2214 21 -10.978880039891887 2214 22 -12.000963692124406 2214 23 -12.032761342029847 2214 24 -10.854903597998284 2214 25 -10.581620566162586 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 149038.0 3 1.0 4 649.0 5 2695.0 6 3836.0 7 3629.0 8 2965.0 9 2827.0 10 2646.0 11 2454.0 12 2392.0 13 2609.0 14 3872.0 15 8375.0 16 12409.0 17 12845.0 18 13372.0 19 15586.0 20 18632.0 21 21646.0 22 22296.0 23 24087.0 24 25592.0 25 26277.0 26 21958.0 27 17449.0 28 18309.0 29 21364.0 30 20618.0 31 23920.0 32 30935.0 33 38944.0 34 47371.0 35 54139.0 36 53868.0 37 56025.0 38 42536.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.194034089327175 19.407724389850046 12.869946042865019 25.52829547795776 2 13.014282810668703 20.25693482790913 42.77966784249587 23.94911451892629 3 18.254000847011717 25.355552418475124 30.166065630574558 26.224381103938605 4 11.06646911533226 18.15465020530028 39.04864468207866 31.7302359972888 5 11.678321873465196 39.47180425949904 36.28848171630129 12.561392150734473 6 30.626629414811855 38.30671071217281 18.808931386952228 12.257728486063108 7 26.143384754869935 32.53823282356235 23.106225250329995 18.212157171237717 8 23.10693487099643 39.33950380634208 20.894848985105845 16.65871233755565 9 26.318798813900045 15.406123186610078 20.61966832235518 37.65540967713469 10 14.228596720740647 29.969556565568634 35.53716171066844 20.264685003022272 11 32.547826131602726 22.199231906678715 25.9988807196254 19.254061242093158 12 23.49959859340975 24.789614213640643 33.09590924363713 18.61487794931248 13 28.203053577984292 22.230364456985725 25.616078434195465 23.950503530834517 14 19.597384657887467 23.503207784633332 28.27590017497007 28.623507382509132 15 21.628355519805957 33.36549967190335 23.633044749395168 21.373100058895528 16 20.393530285361162 28.367919544697944 30.14557107117583 21.092979098765063 17 20.309452477474395 29.166972650634353 29.808232068198535 20.715342803692717 18 20.132824251406642 27.741815960012854 32.56770650576275 19.55765328281775 19 22.608265954498748 26.893167455103256 27.657080356770624 22.84148623362737 20 22.64737938384034 28.440345863204808 29.08871827165278 19.823556481302074 21 21.378902635240085 26.640268271162498 27.017635360346958 24.963193733250456 22 21.96312870567315 29.476772460002067 28.242080236573614 20.318018597751177 23 20.921014457714808 28.83395387070258 29.117789275180115 21.127242396402497 24 22.260435471636956 27.72581572889227 29.431729572405697 20.582019227065075 25 21.796362739598848 27.979512001702094 29.29512968801377 20.928995570685284 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 897.0 1 897.0 2 1883.5 3 2870.0 4 2870.0 5 2870.0 6 4106.0 7 5342.0 8 5342.0 9 5342.0 10 6305.5 11 7269.0 12 7269.0 13 7269.0 14 8996.0 15 10723.0 16 10723.0 17 10723.0 18 15672.5 19 20622.0 20 20622.0 21 20622.0 22 29075.5 23 37529.0 24 37529.0 25 37529.0 26 48869.0 27 60209.0 28 60209.0 29 60209.0 30 72685.0 31 85161.0 32 85161.0 33 85161.0 34 92110.0 35 99059.0 36 99059.0 37 99059.0 38 101086.0 39 103113.0 40 103113.0 41 103113.0 42 101878.5 43 100644.0 44 100644.0 45 100644.0 46 98149.5 47 95655.0 48 95655.0 49 95655.0 50 85863.5 51 76072.0 52 76072.0 53 76072.0 54 64263.0 55 52454.0 56 52454.0 57 52454.0 58 43275.0 59 34096.0 60 34096.0 61 34096.0 62 26822.5 63 19549.0 64 19549.0 65 19549.0 66 14839.0 67 10129.0 68 10129.0 69 10129.0 70 7223.0 71 4317.0 72 4317.0 73 4317.0 74 2990.0 75 1663.0 76 1663.0 77 1663.0 78 1115.0 79 567.0 80 567.0 81 567.0 82 365.5 83 164.0 84 164.0 85 164.0 86 108.0 87 52.0 88 52.0 89 52.0 90 30.5 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.260384995278724 2 36.43339620317666 3 5.911375255685454 4 13.77622360734442 5 13.458895921832095 6 14.685944605308599 7 9.437238428044619 8 7.400448702313304 9 0.1107265934607313 10 1.5160004153756614 11 5.928038581637015 12 5.84520494683433 13 16.12852978750637 14 1.6592084195680576 15 3.0246351576857777 16 3.0831982959937982 17 2.988169038574392 18 1.5822914729655648 19 1.5248150733065593 20 0.29052146550329283 21 4.6148960474107845 22 1.675268001825721 23 0.11181333210974612 24 9.101194688021485 25 0.11471130184045229 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 828166.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.42364859444167 #Duplication Level Percentage of deduplicated Percentage of total 1 96.51928822866293 87.27626201374247 2 2.4703150863526195 4.467498065717942 3 0.4336285583319833 1.1763082913737684 4 0.18492252175610052 0.6688547649788651 5 0.09998432382358519 0.45204736811883656 6 0.06247784581774005 0.33896848650966205 7 0.03791598457255757 0.23999511655708522 8 0.031087539388954866 0.22488389898981748 9 0.019631240685307774 0.1597615568281059 >10 0.11923684159002039 2.0128642312131904 >50 0.011520379579731984 0.7013113729095656 >100 0.009158828673113495 1.6227204794030121 >500 5.550805102935001E-4 0.3726786071576909 >1k 2.7754025514675003E-4 0.285845746500074 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1241 0.1498491848252645 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1037 0.12521644211426214 No Hit CTTTAGGACGTGAAATATGGCGAGG 875 0.1056551464319955 No Hit CTGTAGGACGTGGAATATGGCAAGA 867 0.10468915652176014 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.2074873877942345E-4 13 0.0 0.0 0.0 0.0 2.414974775588469E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTAGA 110 7.410017E-8 14.902417 2 GCAGGAC 75 3.4570567E-6 14.576651 3 ATAAAGC 45 0.0045371163 14.576651 3 GTATTAA 65 8.3498313E-4 14.368617 1 TATAGAA 125 2.2922904E-8 14.20697 2 TAGGACC 280 0.0 14.140807 4 GTCCAGG 85 2.7785698E-5 14.127127 1 GTCCTAC 760 0.0 13.6934 1 GTCCTAA 465 0.0 13.485753 1 GTATAGA 90 4.7572124E-5 13.342287 1 GTTCAAG 70 0.0014614512 13.342286 1 AAGACTA 65 3.7077782E-4 12.8131485 5 TAGAAAT 205 0.0 12.528177 4 GGCGAGG 395 0.0 12.502196 19 TCCTACA 875 0.0 12.333524 2 GGCCATT 60 5.361109E-4 12.26517 11 CCCTGTA 90 5.827003E-4 12.142711 2 GTGTATA 400 0.0 12.008059 1 GTATAAA 90 7.1043085E-4 11.859811 1 CTAGAGT 145 2.224624E-9 11.8081665 4 >>END_MODULE