FastQCFastQC Report
Thu 26 May 2016
SRR1926711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926711_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828166
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA24980.3016303494709998No Hit
GTCCTACAGTGGACATTTCTAAATT24020.2900384705481751No Hit
CTTTAGGACGTGAAATATGGCGAGG23770.28701975207868957No Hit
GTCCTAAAGTGTGTATTTCTCATTT22180.2678207026127612No Hit
GTCCTACAGTGTGCATTTCTCATTT16220.19585445430022483No Hit
GTATCAACGCAGAGTACTTTTTTTT14470.17472342501382573No Hit
CTGTAGGACCTGGAATATGGCGAGA13910.16796149564217802No Hit
CCCATGTACTCTGCGTTGATACCAC13180.1591468377112801No Hit
CTGAAGGACCTGGAATATGGCGAGA13180.1591468377112801No Hit
GTACATGGGAAGCAGTGGTATCAAC11810.14260426049849909No Hit
GGTATCAACGCAGAGTACTTTTTTT9050.10927760859537822No Hit
GATATACACTGTTCTACAAATCCCG8390.10130819183593628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAAC402.6876546E-416.6823061
GACAGTC508.69376E-515.202527
TGCGAGA400.005375161314.21019719
TAGGACC6000.013.6660944
ATTAAGC706.9599155E-613.6256583
CGAACTC500.00152001813.27487410
GTAGGAC14700.013.0417023
TGTAGGA15100.012.8872892
TAGGACT2100.012.7126464
CTGTAGG14200.012.687951
CTAAGAC1001.3663521E-712.399353
TAGGAGT853.7806512E-612.3387434
CTCTTAG1251.3315002E-912.2019151
TCGTCAA550.002973386812.14072
CCTTAGA1103.6257916E-812.13258651
ATAGGAC1900.012.0479513
GGACTTG1900.012.0435586
CGTGGAA7150.011.96147259
GACGTGG7550.011.9556247
ACGTGGA7500.011.783388
Produced by FastQC (version 0.11.5)