FastQCFastQC Report
Thu 26 May 2016
SRR1926710_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926710_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27942
Sequences flagged as poor quality0
Sequence length25
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC3511.2561735022546703No Hit
GTACATGGGAAGCAGTGGTATCAAC3191.1416505618781763No Hit
GAGTACATGGGAAGCAGTGGTATCA1510.5404051249015819No Hit
CATGTACTCTGCGTTGATACCACTG1360.4867224966001002No Hit
GCGTTGATACCACTGCTTCCCATGT1170.4187245007515568No Hit
ACGCAGAGTACATGGGAAGCAGTGG1170.4187245007515568No Hit
GGTATCAACGCAGAGTACATGGGAA1000.3578841886765443No Hit
ACTCTGCGTTGATACCACTGCTTCC960.3435688211294825No Hit
GTATCAACGCAGAGTACTTTTTTTT950.339989979242717No Hit
GTATCAACGCAGAGTACATGGGAAG800.28630735094123544No Hit
CNCATGTACTCTGCGTTGATACCAC780.2791496671677045No Hit
TATCAACGCAGAGTACATGGGAAGC760.27199198339417363No Hit
GNACATGGGAAGCAGTGGTATCAAC680.2433612483000501No Hit
GGTATCAACGCAGAGTACTTTTTTT640.22904588075298832No Hit
GCTTCCCATGTACTCTGCGTTGATA570.20399398754563022No Hit
GTGGTATCAACGCAGAGTACATGGG550.19683630377209932No Hit
GTACTCTGCGTTGATACCACTGCTT510.18252093622503757No Hit
TATCAACGCAGAGTACTTTTTTTTT440.15746904301767947No Hit
CATGGGAAGCAGTGGTATCAACGCA410.14673251735738316No Hit
CAGTGGTATCAACGCAGAGTACATG410.14673251735738316No Hit
GTACTGGTTCACTATCGGTCAGTCA400.14315367547061772No Hit
CTGCTTCCCATGTACTCTGCGTTGA400.14315367547061772No Hit
GATACCACTGCTTCCCATGTACTCT390.13957483358385225No Hit
GGGAAGCAGTGGTATCAACGCAGAG390.13957483358385225No Hit
ACGCAGAGTACTTTTTTTTTTTTTT380.13599599169708682No Hit
CCATGTACTCTGCGTTGATACCACT370.13241714981032138No Hit
GTTGATACCACTGCTTCCCATGTAC350.1252594660367905No Hit
ATACCACTGCTTCCCATGTACTCTG350.1252594660367905No Hit
ACATGGGAAGCAGTGGTATCAACGC330.11810178226325961No Hit
GCAGAGTACATGGGAAGCAGTGGTA330.11810178226325961No Hit
GNGTACATGGGAAGCAGTGGTATCA320.11452294037649416No Hit
CCACTGCTTCCCATGTACTCTGCGT310.11094409848972872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA300.1073652566029633No Hit
TACCACTGCTTCCCATGTACTCTGC300.1073652566029633No Hit
CTTCCCATGTACTCTGCGTTGATAC300.1073652566029633No Hit
AAGCAGTGGTATCAACGCAGAGTAC280.1002075728294324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTCT1901.6794493E-68.2236846
TGTACTC1901.6794493E-68.2236845
ATACCAC2001.3420504E-88.1074319
CCATGTA1550.00538706137.88415962
CATGGGA2353.1975105E-77.76380874
AGTGGTA1753.483201E-77.76047314
CAGTGGT1556.042865E-57.60024813
TACTCTG1756.410298E-57.55297337
ATGTACT1609.2044444E-47.37847234
TTGATAC1907.8889207E-77.36944116
GATACCA2102.707784E-77.31497418
GTGGTAT1802.9294642E-67.30711315
CATGTAC1650.00105412567.2836333
TGATACC1951.5723363E-67.057387417
TGGGAAG1951.8070247E-46.94444476
ATGGGAA2157.8930425E-56.7829455
TGGTATC1959.395764E-66.758116
TACATGG3453.1300497E-66.69074442
ACATGGG3601.3576937E-86.67666343
GTTGATA2103.9498555E-66.654692615
Produced by FastQC (version 0.11.5)