FastQCFastQC Report
Thu 26 May 2016
SRR1926710_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926710_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27942
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC9783.500107365256603No Hit
GTACATGGGAAGCAGTGGTATCAAC8463.0277002362035645No Hit
GTATCAACGCAGAGTACTTTTTTTT3811.3635387588576338No Hit
GAGTACATGGGAAGCAGTGGTATCA3441.2311216090473123No Hit
CATGTACTCTGCGTTGATACCACTG3401.2168062415002505No Hit
GGTATCAACGCAGAGTACTTTTTTT3061.0951256173502255No Hit
GCGTTGATACCACTGCTTCCCATGT2911.0414429890487438No Hit
ACGCAGAGTACATGGGAAGCAGTGG2720.9734449932002004No Hit
GTATCAACGCAGAGTACATGGGAAG2220.7945028988619284No Hit
GGTATCAACGCAGAGTACATGGGAA2160.7730298475413356No Hit
ACTCTGCGTTGATACCACTGCTTCC2110.7551356381075084No Hit
TATCAACGCAGAGTACTTTTTTTTT2080.744399112447212No Hit
TATCAACGCAGAGTACATGGGAAGC1920.687137642258965No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1740.622718488297187No Hit
GCTTCCCATGTACTCTGCGTTGATA1220.436618710185384No Hit
CATGGGAAGCAGTGGTATCAACGCA1160.4151456588647914No Hit
GCAGAGTACATGGGAAGCAGTGGTA1140.40798797509126045No Hit
GTGGTATCAACGCAGAGTACATGGG1130.404409133204495No Hit
CAGTGGTATCAACGCAGAGTACATG1090.39009376565743326No Hit
GGGAAGCAGTGGTATCAACGCAGAG1000.3578841886765443No Hit
GTACTCTGCGTTGATACCACTGCTT990.3543053467897788No Hit
GATACCACTGCTTCCCATGTACTCT990.3543053467897788No Hit
ATACCACTGCTTCCCATGTACTCTG980.3507265049030134No Hit
CTGCTTCCCATGTACTCTGCGTTGA920.3292534535824207No Hit
ACATGGGAAGCAGTGGTATCAACGC910.3256746116956553No Hit
CCATGTACTCTGCGTTGATACCACT870.3113592441485935No Hit
AAGCAGTGGTATCAACGCAGAGTAC820.2934650347147663No Hit
TACCACTGCTTCCCATGTACTCTGC810.2898861928280008No Hit
GCAGTGGTATCAACGCAGAGTACAT720.2576766158471119No Hit
GTTGATACCACTGCTTCCCATGTAC720.2576766158471119No Hit
CCACTGCTTCCCATGTACTCTGCGT700.250518932073581No Hit
GTACTGGTTCACTATCGGTCAGTCA680.2433612483000501No Hit
CTTCCCATGTACTCTGCGTTGATAC680.2433612483000501No Hit
GCAGAGTACTTTTTTTTTTTTTTTT620.22188819697945744No Hit
GAGTACTTTTTTTTTTTTTTTTTTT550.19683630377209932No Hit
ATCAACGCAGAGTACTTTTTTTTTT550.19683630377209932No Hit
ATGGGAAGCAGTGGTATCAACGCAG530.18967861999856847No Hit
GGATACCACGTGTCCCGCCCTACTC480.17178441056474125No Hit
ATCAACGCAGAGTACATGGGAAGCA480.17178441056474125No Hit
AAAAAGTACTCTGCGTTGATACCAC440.15746904301767947No Hit
GTACTTTTTTTTTTTTTTTTTTTTT410.14673251735738316No Hit
GTACATGGTAAGCAGTGGTATCAAC410.14673251735738316No Hit
GTGGTATCAACGCAGAGTACTTTTT380.13599599169708682No Hit
CCCATATTCAGACAGGATACCACGT370.13241714981032138No Hit
ACTTAGATGTTTCAGTTCCCCCGGT350.1252594660367905No Hit
CTATCGGTCAGTCAGGAGTATTTAG330.11810178226325961No Hit
GGAAGCAGTGGTATCAACGCAGAGT320.11452294037649416No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA320.11452294037649416No Hit
ACCATGTACTCTGCGTTGATACCAC290.10378641471619784No Hit
GTTCACTATCGGTCAGTCAGGAGTA280.1002075728294324No Hit
CAACGCAGAGTACTTTTTTTTTTTT280.1002075728294324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGTA2300.010.384072
ATGTACT2600.09.1859094
TACTCTG2700.09.1498797
GTACTCT2750.09.0322546
CCCATGT2650.09.012591
TGTACTC2650.09.012595
ACTCTGC2900.08.8465028
CATGTAC2700.08.845693
TGCGTTG2900.08.81479512
CTGCGTT2900.08.81479511
TCTGCGT2950.08.66539110
CTCTGCG2900.08.5188549
CAGTGGT2501.7644197E-108.33161313
AGTGGTA2501.6043487E-97.952903314
GATACCA3103.6379788E-127.940686718
TGATACC3103.6379788E-127.940686717
ATACCAC3155.456968E-127.81464419
TGGTATC2552.3628672E-97.79696416
TTGATAC3207.2759576E-127.6925416
CGTTGAT3207.2759576E-127.6925414
Produced by FastQC (version 0.11.5)