FastQCFastQC Report
Thu 26 May 2016
SRR1926709_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926709_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831611
Sequences flagged as poor quality0
Sequence length25
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14290.171835148885717No Hit
GTCCTAAAGTGTGTATTTCTCATTT11800.14189326500010221No Hit
CTGTAGGACGTGGAATATGGCAAGA10530.12662170173314205No Hit
CTTTAGGACGTGAAATATGGCGAGG9220.11086914434753749No Hit
GTCCTACAGTGTGCATTTCTCATTT8530.10257199580092134No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGTGG300.00766736718.2240493
GTATAAC500.002447715.9915171
GTATAAT953.0365118E-715.4304111
TCCTACA10250.014.5215572
TAGGACC3400.014.48070054
CCCCTAA658.4262545E-414.3513611
GTCCTAC9650.014.2238881
CATTAAC700.001226693413.6555922
GTCCTAT1008.735418E-613.3262641
CTATAGA904.811312E-513.3262641
GTATAAA1008.735418E-613.3262641
GTTATAT1008.735418E-613.3262641
TGTAGGA9550.013.1551272
ATTTAGA2600.012.8137161
GTATCAA4900.012.2384061
CTGTAGG9500.012.2040521
CCTACAG11150.012.1602623
GGACCTG5650.012.1587966
AGGACCT5950.012.07753855
TAGGACA3000.011.7734384
Produced by FastQC (version 0.11.5)