##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926709_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 831611 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 21.74631167697397 27.0 10.0 31.0 2.0 31.0 2 16.758757399793893 22.0 2.0 31.0 2.0 31.0 3 20.475111560573392 25.0 15.0 31.0 2.0 31.0 4 22.507851627744223 28.0 7.0 35.0 2.0 37.0 5 23.089992797113073 30.0 7.0 35.0 2.0 37.0 6 24.03274367462672 31.0 8.0 35.0 2.0 37.0 7 23.676692588241377 31.0 8.0 35.0 2.0 37.0 8 23.96464212233845 31.0 12.0 35.0 2.0 37.0 9 26.81657048788436 32.0 17.0 37.0 2.0 39.0 10 27.607081916905862 32.0 25.0 38.0 2.0 39.0 11 24.605248126828528 30.0 8.0 37.0 2.0 39.0 12 25.82998300888276 31.0 17.0 37.0 2.0 39.0 13 22.354428933720214 28.0 2.0 37.0 2.0 39.0 14 25.830647983251783 31.0 17.0 38.0 2.0 40.0 15 26.978072680616297 31.0 18.0 38.0 2.0 40.0 16 27.343124369446773 32.0 20.0 39.0 2.0 40.0 17 26.661675951857298 31.0 17.0 38.0 2.0 40.0 18 26.769057888844664 31.0 17.0 38.0 2.0 40.0 19 27.164079118722576 31.0 21.0 39.0 2.0 40.0 20 27.46037630574872 31.0 24.0 39.0 2.0 40.0 21 25.238493718818052 30.0 10.0 38.0 2.0 40.0 22 26.213503669383883 31.0 16.0 38.0 2.0 40.0 23 26.21831962299681 31.0 10.0 39.0 2.0 40.0 24 25.21577155665329 31.0 2.0 39.0 2.0 40.0 25 25.034512530498034 31.0 2.0 39.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.568845132392056 1101 2 10.293043223510107 1101 3 6.8852054426455105 1101 4 8.28882550200499 1101 5 7.784425053044064 1101 6 7.606880600019366 1101 7 7.236216189540606 1101 8 5.888414718332033 1101 9 5.576056480318137 1101 10 5.139856188999204 1101 11 8.056444469407044 1101 12 5.51042408437484 1101 13 10.121879958844652 1101 14 7.303060249904391 1101 15 6.325278004772013 1101 16 6.135980153104068 1101 17 2.2304863279067426 1101 18 4.610884321091007 1101 19 5.2624160098601855 1101 20 3.177354376420624 1101 21 0.34477738894765153 1101 22 4.119077681780702 1101 23 5.470979059962186 1101 24 6.838291738254306 1101 25 7.32036415338537 1102 1 5.793243465070642 1102 2 10.928179094314768 1102 3 7.1485089225094995 1102 4 8.253236753040444 1102 5 8.168232672520475 1102 6 7.04629895026574 1102 7 6.9294964369609815 1102 8 7.145849026721756 1102 9 6.1868637599152265 1102 10 5.322522932175495 1102 11 8.191614916679402 1102 12 5.635226005355104 1102 13 10.099168369254084 1102 14 7.830650064118409 1102 15 5.428348247969641 1102 16 5.903864647103216 1102 17 6.874384022759855 1102 18 6.975595431025045 1102 19 5.774416986829319 1102 20 5.9274636233451545 1102 21 4.836175205186233 1102 22 5.820353185603977 1102 23 6.3547256188679775 1102 24 7.920245323392603 1102 25 7.699779825794675 1103 1 4.9391101865280795 1103 2 9.477785285671523 1103 3 6.232357060764869 1103 4 7.58727780045729 1103 5 6.955114849540312 1103 6 6.469416088361303 1103 7 6.505297181202245 1103 8 5.9948000118141 1103 9 5.059004937460141 1103 10 4.3405536606644155 1103 11 5.204253320441705 1103 12 4.086017047064576 1103 13 8.326362853650128 1103 14 6.423472473542418 1103 15 5.4341104103786115 1103 16 4.991201130905694 1103 17 2.6079228214722683 1103 18 1.6441021543088397 1103 19 3.799858166657181 1103 20 4.224269778174737 1103 21 6.311768201099756 1103 22 1.9979620251811738 1103 23 4.19956273048134 1103 24 5.661432000104245 1103 25 0.8954056961688472 1104 1 5.753336631267118 1104 2 10.549376585322921 1104 3 7.095940308174896 1104 4 7.511174299696254 1104 5 7.4011501026458255 1104 6 7.151143883323744 1104 7 7.031803294031821 1104 8 7.07513093929483 1104 9 5.893334981158283 1104 10 4.918216055715234 1104 11 7.930327361783089 1104 12 4.910564108487467 1104 13 9.44273738107194 1104 14 7.056548036953821 1104 15 6.181171833268582 1104 16 5.800355645561755 1104 17 6.1220829825170995 1104 18 3.6135846505036646 1104 19 4.52867684392924 1104 20 3.9928709467317134 1104 21 3.2424059975351653 1104 22 5.090827935722736 1104 23 5.449484172028942 1104 24 6.520515321162822 1104 25 5.602397398432313 1105 1 4.102836816383739 1105 2 10.249765330232215 1105 3 6.941719649159715 1105 4 6.351316839496327 1105 5 2.5365906802096916 1105 6 5.384150177288941 1105 7 6.302536355860774 1105 8 3.579925459842773 1105 9 4.393520097781753 1105 10 3.958775107918118 1105 11 7.438565051527629 1105 12 4.4293256782764345 1105 13 5.7141183510830444 1105 14 4.172273694117834 1105 15 4.624498378992385 1105 16 4.940692557816476 1105 17 5.634661657082074 1105 18 3.402135187341873 1105 19 3.0546024270466035 1105 20 2.1312178552841026 1105 21 3.231697000867264 1105 22 4.603415866118887 1105 23 1.870615234598361 1105 24 5.139313914276482 1105 25 5.825111208132423 1106 1 6.80914924294073 1106 2 11.579094795567116 1106 3 8.04814958452987 1106 4 9.459032027510428 1106 5 8.8108452336762 1106 6 7.941163984574487 1106 7 7.737381391113416 1106 8 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1.5567806767235162 2207 6 2.1249402545352325 2207 7 3.4462603242283265 2207 8 3.779959404174072 2207 9 1.3424714931689934 2207 10 0.9354379214444783 2207 11 -1.482588688770715 2207 12 -2.329592965418179 2207 13 0.5204485101626943 2207 14 -5.504464806093171 2207 15 -1.4260039053693703 2207 16 -0.19125731466292706 2207 17 1.0011397950978882 2207 18 0.9616400588121223 2207 19 0.32252641009278804 2207 20 0.3423887512309669 2207 21 -4.176841833282566 2207 22 -4.73150092439872 2207 23 -1.5451335590017585 2207 24 0.2939601592995089 2207 25 0.8790102191597455 2208 1 4.334635119541268 2208 2 5.985263449710917 2208 3 5.7771390491422245 2208 4 7.851786969820825 2208 5 3.3692220980352836 2208 6 5.7646452354539015 2208 7 6.117991616947101 2208 8 6.137368825538566 2208 9 4.580820019062255 2208 10 3.8669969219651747 2208 11 6.237812843008427 2208 12 5.671088557660671 2208 13 8.271207479045959 2208 14 2.674291974534171 2208 15 4.08232003560045 2208 16 4.621371294805893 2208 17 5.244328848545383 2208 18 0.5032175804145567 2208 19 2.739079379727439 2208 20 3.730056742715224 2208 21 6.5397460693532246 2208 22 5.306477179859812 2208 23 1.2177135465024982 2208 24 2.820996324842387 2208 25 4.18030669488131 2209 1 3.5649804668835543 2209 2 5.300061516829771 2209 3 5.6747074314625685 2209 4 0.9865167654496787 2209 5 5.554574760180071 2209 6 6.111417855926483 2209 7 6.647556718689355 2209 8 5.121021350253734 2209 9 4.900869227733622 2209 10 3.9230105762631773 2209 11 0.0892314713721305 2209 12 3.837961368761441 2209 13 2.9776688879212543 2209 14 4.486000091748341 2209 15 4.717473203131593 2209 16 4.441451535972714 2209 17 1.8885213386814819 2209 18 3.9868723217365414 2209 19 4.612923419271706 2209 20 4.779857248101578 2209 21 7.034186819452973 2209 22 5.51891012750356 2209 23 0.8357855418919549 2209 24 -2.6377038569193694 2209 25 0.6715754806240959 2210 1 -2.238058193247493 2210 2 -14.221856291389408 2210 3 -7.8760504721739615 2210 4 -2.4003130232834238 2210 5 -0.5364602362468105 2210 6 -1.2608723617820914 2210 7 -1.3168671034398187 2210 8 -21.370074540157226 2210 9 -13.525883361518842 2210 10 -7.649885058493702 2210 11 -10.714712546423005 2210 12 -5.140168632345723 2210 13 -5.457583696298649 2210 14 -4.4560942586990535 2210 15 -6.301752688386529 2210 16 -5.752295507397893 2210 17 -5.127293483216196 2210 18 -5.230810083424853 2210 19 -10.821162114129399 2210 20 -7.848994925461373 2210 21 -5.841940725764584 2210 22 -10.516827595564692 2210 23 -8.517897286004533 2210 24 -10.978616562122031 2210 25 -8.486655775603701 2211 1 0.19492175846868065 2211 2 5.031715137182022 2211 3 0.8371829696230364 2211 4 1.8324365306160182 2211 5 1.2173242709432799 2211 6 0.19789535103411637 2211 7 -21.083950130625713 2211 8 -13.442217397300084 2211 9 -9.197966388704735 2211 10 -7.231128366985356 2211 11 -9.805797882835307 2211 12 -4.962508298557545 2211 13 -7.096499409534715 2211 14 -4.842610475766339 2211 15 -5.325520926026918 2211 16 -11.743284276160358 2211 17 -7.774952242531828 2211 18 -5.96153276632608 2211 19 -8.714258576814398 2211 20 -6.8408863918201455 2211 21 -4.557916898746637 2211 22 -6.050522357819336 2211 23 -8.316102912119785 2211 24 -22.68838878842622 2211 25 -22.516780683759468 2212 1 -0.9391441755972529 2212 2 -14.221856291389408 2212 3 -7.9673042098641425 2212 4 -19.968277755098267 2212 5 -20.426247157628147 2212 6 -21.3563903632516 2212 7 -9.922777969453552 2212 8 -9.57447014455283 2212 9 -5.013112908851255 2212 10 -5.578033128890116 2212 11 -5.764662351699776 2212 12 -2.931116357165603 2212 13 -6.258221021256327 2212 14 -3.9141855673414305 2212 15 -3.878048398554391 2212 16 -3.9511597340358158 2212 17 -3.9619302645098493 2212 18 -3.143932033725349 2212 19 -3.777005829561652 2212 20 -3.945465146398899 2212 21 -1.7751513309810676 2212 22 -3.1571523944493727 2212 23 -3.3890150000318755 2212 24 -2.3074364074738405 2212 25 -2.7387587057374887 2213 1 -19.233649670102746 2213 2 -14.221856291389408 2213 3 -8.813433560037039 2213 4 -5.758324411396867 2213 5 -20.426247157628147 2213 6 -10.943482120638846 2213 7 -21.083950130625713 2213 8 -10.844413575927055 2213 9 -8.546465611099759 2213 10 -7.229169913308397 2213 11 -6.67338064222211 2213 12 -5.190659820441567 2213 13 -19.675070838106144 2213 14 -12.691106365568604 2213 15 -7.1298488531542255 2213 16 -6.405148306375867 2213 17 -5.00269385550672 2213 18 -5.158714503717771 2213 19 -5.960450534157211 2213 20 -5.9899088312780435 2213 21 -3.961023986060564 2213 22 -4.82665432849252 2213 23 -4.646070697056466 2213 24 -3.578746486715488 2213 25 -4.449129050789015 2214 1 -19.233649670102746 2214 2 -14.221856291389408 2214 3 -12.494759960220655 2214 4 -19.968277755098267 2214 5 -20.426247157628147 2214 6 -21.3563903632516 2214 7 -16.679369977953957 2214 8 -15.09950626195536 2214 9 -13.884993530350195 2214 10 -13.808107041406778 2214 11 -14.801432197629927 2214 12 -12.425761660971212 2214 13 -19.675070838106144 2214 14 -16.988828820610635 2214 15 -24.462663783169777 2214 16 -18.55767298329716 2214 17 -24.197095099674684 2214 18 -18.113997930508802 2214 19 -15.20151533193375 2214 20 -13.795539245327959 2214 21 -11.160534734685083 2214 22 -12.226350541510117 2214 23 -12.217690819547387 2214 24 -11.005606769766338 2214 25 -10.752573728712818 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 136467.0 3 1.0 4 631.0 5 2456.0 6 3611.0 7 3400.0 8 2833.0 9 2721.0 10 2395.0 11 2289.0 12 2200.0 13 2351.0 14 3834.0 15 8551.0 16 13005.0 17 13543.0 18 13405.0 19 15734.0 20 18806.0 21 21975.0 22 22979.0 23 24677.0 24 25949.0 25 26874.0 26 22269.0 27 17379.0 28 18215.0 29 21260.0 30 20708.0 31 24051.0 32 31332.0 33 40080.0 34 49085.0 35 56304.0 36 56124.0 37 58906.0 38 45211.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.6163071870518 19.681560878271174 13.28631113636206 25.415820798314957 2 12.68592899224689 20.277659006890396 43.46945107669488 23.566960924167837 3 18.05420194740748 25.392936656247965 30.40630384717079 26.146557549173764 4 10.89253619048946 18.428657015869476 39.397127053348754 31.28167974029231 5 11.51129227044748 39.6432279036026 36.51549621089467 12.329983615055248 6 30.265273877887832 38.678333948240756 18.911501209630856 12.14489096424055 7 26.090526340962445 32.762223872605745 23.145743184085816 18.001506602345994 8 22.92357078299172 39.72316874266302 20.882333080543116 16.470927393802135 9 26.191917890836514 15.560028361656869 20.97618993161181 37.271863815894804 10 14.179496129802352 30.04571983723445 35.69039883418076 20.08438519878243 11 32.225953941022944 22.27295315791558 26.423038942025784 19.078053959035692 12 23.5594439851811 24.79192819162508 33.1808834185522 18.46774440464162 13 28.139038505615403 22.5191955076782 25.68874627549851 23.653019711207886 14 19.209323359734036 24.026718490270852 28.40923535738731 28.354722792607802 15 21.201217206993363 34.08080419948808 23.778299752716094 20.939678840802465 16 20.03375918858885 28.543009479113827 30.68811941971899 20.735111912578336 17 19.994473310177412 29.636812215223674 30.190596448791997 20.178118025806917 18 19.899072058934884 27.83261398970691 33.09652394596007 19.171790005398133 19 22.461900123765094 26.953206053016377 28.010091615464045 22.574802207754484 20 22.565426652564906 28.678104849067367 29.413743908365902 19.34272459000182 21 20.988354221153894 26.93294228511245 27.187551967587343 24.89115152614631 22 21.765969982862018 30.070189935775353 28.43328044436512 19.730559636997505 23 20.64327478340217 29.317059253364985 29.47066705028488 20.568998912947958 24 22.05928241254932 27.84512211891389 29.98035202403241 20.115243444504387 25 21.537002125963966 28.353088185370574 29.745678851835 20.364230836830462 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 913.0 1 913.0 2 1921.5 3 2930.0 4 2930.0 5 2930.0 6 4240.5 7 5551.0 8 5551.0 9 5551.0 10 6563.0 11 7575.0 12 7575.0 13 7575.0 14 9650.5 15 11726.0 16 11726.0 17 11726.0 18 16958.0 19 22190.0 20 22190.0 21 22190.0 22 31112.0 23 40034.0 24 40034.0 25 40034.0 26 51548.0 27 63062.0 28 63062.0 29 63062.0 30 75565.0 31 88068.0 32 88068.0 33 88068.0 34 94584.5 35 101101.0 36 101101.0 37 101101.0 38 102776.5 39 104452.0 40 104452.0 41 104452.0 42 103161.5 43 101871.0 44 101871.0 45 101871.0 46 97681.5 47 93492.0 48 93492.0 49 93492.0 50 84112.5 51 74733.0 52 74733.0 53 74733.0 54 62194.0 55 49655.0 56 49655.0 57 49655.0 58 40681.0 59 31707.0 60 31707.0 61 31707.0 62 24821.5 63 17936.0 64 17936.0 65 17936.0 66 13411.5 67 8887.0 68 8887.0 69 8887.0 70 6305.0 71 3723.0 72 3723.0 73 3723.0 74 2535.0 75 1347.0 76 1347.0 77 1347.0 78 920.0 79 493.0 80 493.0 81 493.0 82 315.0 83 137.0 84 137.0 85 137.0 86 78.5 87 20.0 88 20.0 89 20.0 90 14.0 91 8.0 92 8.0 93 8.0 94 4.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.223249812712915 2 36.441196665267775 3 5.85898935920761 4 13.694022806336136 5 13.473607251467332 6 14.607430637641878 7 9.490494954972938 8 7.399974266814653 9 0.10966665905092646 10 1.4758102045307242 11 5.87197620041101 12 5.741506545728712 13 16.056906414176822 14 1.6174629724715042 15 2.9876949679597793 16 3.0437307827818536 17 2.9602783031970477 18 1.5402634164290756 19 1.4811011398358127 20 0.28859647118664855 21 4.551046102083787 22 1.628766334259648 23 0.1111096414068597 24 9.054594034951439 25 0.1117108840551652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 831611.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.4680420142263 #Duplication Level Percentage of deduplicated Percentage of total 1 96.10584480466616 85.98401760796584 2 2.7593590095951415 4.93748895605584 3 0.5010170605195285 1.3447504626121602 4 0.20281861303363694 0.7258313676864213 5 0.10583996037928424 0.47346470109989247 6 0.07019827921620042 0.3768301556544845 7 0.04371917764906642 0.27380284559138685 8 0.030684564494625248 0.21962303243146944 9 0.025391237185509855 0.2044533847775743 >10 0.13230169692524188 2.2046216546263593 >50 0.013024076055825996 0.8214044979864993 >100 0.008821368293611678 1.6204280824035169 >500 5.600868492352915E-4 0.3545273568449157 >1k 4.2006513692646855E-4 0.45875589426363295 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1429 0.171835148885717 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1180 0.14189326500010221 No Hit CTGTAGGACGTGGAATATGGCAAGA 1053 0.12662170173314205 No Hit CTTTAGGACGTGAAATATGGCGAGG 922 0.11086914434753749 No Hit GTCCTACAGTGTGCATTTCTCATTT 853 0.10257199580092134 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.2024852966110357E-4 0.0 5 0.0 0.0 0.0 1.2024852966110357E-4 0.0 6 0.0 0.0 0.0 1.2024852966110357E-4 0.0 7 0.0 0.0 0.0 1.2024852966110357E-4 0.0 8 0.0 0.0 0.0 1.2024852966110357E-4 0.0 9 0.0 0.0 0.0 2.4049705932220714E-4 0.0 10 0.0 0.0 0.0 2.4049705932220714E-4 0.0 11 0.0 0.0 0.0 2.4049705932220714E-4 0.0 12 0.0 0.0 0.0 2.4049705932220714E-4 0.0 13 0.0 0.0 0.0 2.4049705932220714E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGTGG 30 0.007667367 18.224049 3 GTATAAC 50 0.0024477 15.991517 1 GTATAAT 95 3.0365118E-7 15.430411 1 TCCTACA 1025 0.0 14.521557 2 TAGGACC 340 0.0 14.4807005 4 CCCCTAA 65 8.4262545E-4 14.351361 1 GTCCTAC 965 0.0 14.223888 1 CATTAAC 70 0.0012266934 13.655592 2 GTCCTAT 100 8.735418E-6 13.326264 1 CTATAGA 90 4.811312E-5 13.326264 1 GTATAAA 100 8.735418E-6 13.326264 1 GTTATAT 100 8.735418E-6 13.326264 1 TGTAGGA 955 0.0 13.155127 2 ATTTAGA 260 0.0 12.813716 1 GTATCAA 490 0.0 12.238406 1 CTGTAGG 950 0.0 12.204052 1 CCTACAG 1115 0.0 12.160262 3 GGACCTG 565 0.0 12.158796 6 AGGACCT 595 0.0 12.0775385 5 TAGGACA 300 0.0 11.773438 4 >>END_MODULE