FastQCFastQC Report
Thu 26 May 2016
SRR1926709_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926709_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831611
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA29380.3532901801443223No Hit
GTCCTACAGTGGACATTTCTAAATT27740.3335694212799013No Hit
CTTTAGGACGTGAAATATGGCGAGG27250.3276772433265072No Hit
GTCCTAAAGTGTGTATTTCTCATTT24900.2994188388561479No Hit
GTATCAACGCAGAGTACTTTTTTTT18960.22799121223745236No Hit
GTCCTACAGTGTGCATTTCTCATTT18160.21837132986456406No Hit
CTGTAGGACCTGGAATATGGCGAGA15650.1881889489196271No Hit
CTGAAGGACCTGGAATATGGCGAGA14970.18001204890267206No Hit
GGTATCAACGCAGAGTACTTTTTTT11580.13924779734755793No Hit
GTACATGGGAAGCAGTGGTATCAAC9640.11591958259330383No Hit
TATCAACGCAGAGTACTTTTTTTTT9600.11543858847465943No Hit
CCCATGTACTCTGCGTTGATACCAC9450.11363486052974286No Hit
GAATATGGCAAGAAAACTGAAAATC9030.10858442228397652No Hit
GTCCTTCAGTGTGCATTTCTCATTT8430.10136951050431031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1101.9463187E-1013.77781718
CCAACGA1205.4569682E-1113.41821319
TAGGACC5600.013.2877754
ACGAAAT1403.6379788E-1212.86447812
GTTAAAG603.9381778E-412.7230023
TATGTCG1001.4951547E-712.31392316
AGGACCT11100.011.9464135
GGCGAGG6950.011.85663519
ATGCCGG802.9514038E-511.83959919
GTATTAA1052.5800364E-711.8077791
GCATTAG657.7588606E-411.7379111
CTATTAC657.7588606E-411.7379111
TAGGACA4500.011.6599274
GACGAAA1552.5465852E-1111.61952911
GTAGGAC16600.011.6116563
GTATAAA1156.683695E-811.6103251
TGTAGGA17100.011.609062
GTCGAGG907.700455E-611.57649719
CTGTAGG16450.011.5372251
TGTCGAG1157.34708E-811.53143318
Produced by FastQC (version 0.11.5)